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The map label for this gene is sapA [H]

Identifier: 218884178

GI number: 218884178

Start: 832031

End: 833773

Strand: Reverse

Name: sapA [H]

Synonym: DKAM_0867

Alternate gene names: 218884178

Gene position: 833773-832031 (Counterclockwise)

Preceding gene: 218884182

Following gene: 218884177

Centisome position: 61.07

GC content: 48.42

Gene sequence:

>1743_bases
ATGAATGCCAGGGGTACAATTGTCAAAGCCGTAGCAGTAATAGTGGCTTTACTACTGGTTGCACCATCAGTCAGCACAGT
ATCTACTGCGCAGTCCCAGAGCGTTTTCAGGGTAGGGTGGGGTGGTACCTCGCTAGATACATTCAACCCGTTTACAACCT
ATGCACAGATATCAACATGGATAACCCTTGACATCTATAGCAGGTTGGTAAGGGTCTCCAGCAATTATTCAACATATATC
CCGGACCTGGCTGCTTCATGGGAGGTTATCGGTGGAGACACTGTTAGATTCCATCTTGTAACCAATGCAACATTCCACGA
TGGAGTACCGGTCACGGCTTCCGATGTAGAGTACAGCTTCCACCTAGCTAGTGAACCATGGTCATCCAGGGCGGCGAACG
TCAAGATCGTTGAATCTATAAGAGTCATAGATAACTACACCATAGACTTCACCGTGTCATCTATACCGTTATTCTTCGCC
CTTGCAGCCAGCAATATACCAATAGTGCCGAAGCATATATGGAGTAATATAAGTGATCCATCAACATATAGCGGGTACCC
ACCCATAGGTTCCGGCCCCCTCAGGCTCACCGAGTACAAGGAGGGACAATACGTTGTGCTGGAGCCGTATAACAGGTTCT
ACTATCCATGGTATCTACCTAGGGTAGACCGGATCATAGTTAAGTTCTACCCCGATGTAACCAGTGCAGCCAACGCGCTG
ATAGCCGGGGACATAGATGCAGTTGGACCCTATATCCCGATGGCGATGCGTAATGTCCTCGTGAACAACCCGGGGTTCAA
GGTGTTTACATCCCCTGGGACAACATACTTCTACATAGCATTCAACGTGGATCCCAATGGAACAGGTAATCCAACCCTGA
AGGACTTGAATGTTAGAAGGGCCCTAGCCTATGCTACAAATGTAACTTACGTCTGTGATACAGCGTGGCACGGGTATTCA
AAGCCGATAGCTTCTGTTCTCCCAACCTCGAATATATATTATAACCCGAACCTCAAACCCTACGAGTTCAATCTTAACAG
GGCAGCAGAAATACTGGATCAAGCCGGCTACAAGCTGGGCCCTAACGGTGTAAGGGCTTCCAGCAATGGTGTTCCGCTCT
CATACACTATACTCGTGCCCAGCAAGTTCCCCGAAGCAGTTAACGCGGCACAGGTAATCGCGAACTGGTGGAAGCAAATA
GGCGTCAACGTGGAGATCAAGACGATGGATACTGGTAGCATGAGCGCCATTATATGGAATAATGTGAATGGAGTTGTCAG
GCTTGGACACGATATAGATATATGGGACTGGATAGTAAGCCCCAACGATGTATCACAATTCGACGTGTTTGCAACCGGGC
GCACCCTCACAGGAGTCTCCGATTCAGGGTACAGTAACCCCGAGTATGATTCCCTCTATGAGAAAATATATAATGCGTCA
AGTATGGATGAGATCAAGAATATAGCGTGGATGCTCCAGGAGATACTGTACAACGACCTACCATACATACCTCTCTGTGA
AATAGAGGCTGTACAAGCACACTCAGTGAAATTCACCGGCTTCGACTACGGGTGGCCTGGCGGGCCATTCGGAGGTGATG
ACTGGCGTACATTTCTCAACACAACGCCCGTAGCCACTACTACAGCCCAGGGTGGCAACAGCGACTACCTAGTGATAGGT
GTAGCAGCAGGAGTTATCGCAGTGGTTGCAATAGTGGTCTTAATTAATAGATTCAGGAGGTGA

Upstream 100 bases:

>100_bases
ATATTCCAAAATGTAACACTTCTTTTTAAAATGTTTCATATATGTAAAAAAGTTTTTATCTCTGTTCCATGAAGCCTTTT
CTAACCATTGGTGGATAACT

Downstream 100 bases:

>100_bases
TACCTGTGAACACCACGTTCCTTACTAGAAGGGCTTTTTCAAGCGTTCTCTCCATCTTCATATTACTATCTATATCATTC
ATTCTTTTCAGGCTTCTCCC

Product: Oligopeptide/dipeptide ABC transporter, periplasmic peptide-binding protein

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MNARGTIVKAVAVIVALLLVAPSVSTVSTAQSQSVFRVGWGGTSLDTFNPFTTYAQISTWITLDIYSRLVRVSSNYSTYI
PDLAASWEVIGGDTVRFHLVTNATFHDGVPVTASDVEYSFHLASEPWSSRAANVKIVESIRVIDNYTIDFTVSSIPLFFA
LAASNIPIVPKHIWSNISDPSTYSGYPPIGSGPLRLTEYKEGQYVVLEPYNRFYYPWYLPRVDRIIVKFYPDVTSAANAL
IAGDIDAVGPYIPMAMRNVLVNNPGFKVFTSPGTTYFYIAFNVDPNGTGNPTLKDLNVRRALAYATNVTYVCDTAWHGYS
KPIASVLPTSNIYYNPNLKPYEFNLNRAAEILDQAGYKLGPNGVRASSNGVPLSYTILVPSKFPEAVNAAQVIANWWKQI
GVNVEIKTMDTGSMSAIIWNNVNGVVRLGHDIDIWDWIVSPNDVSQFDVFATGRTLTGVSDSGYSNPEYDSLYEKIYNAS
SMDEIKNIAWMLQEILYNDLPYIPLCEIEAVQAHSVKFTGFDYGWPGGPFGGDDWRTFLNTTPVATTTAQGGNSDYLVIG
VAAGVIAVVAIVVLINRFRR

Sequences:

>Translated_580_residues
MNARGTIVKAVAVIVALLLVAPSVSTVSTAQSQSVFRVGWGGTSLDTFNPFTTYAQISTWITLDIYSRLVRVSSNYSTYI
PDLAASWEVIGGDTVRFHLVTNATFHDGVPVTASDVEYSFHLASEPWSSRAANVKIVESIRVIDNYTIDFTVSSIPLFFA
LAASNIPIVPKHIWSNISDPSTYSGYPPIGSGPLRLTEYKEGQYVVLEPYNRFYYPWYLPRVDRIIVKFYPDVTSAANAL
IAGDIDAVGPYIPMAMRNVLVNNPGFKVFTSPGTTYFYIAFNVDPNGTGNPTLKDLNVRRALAYATNVTYVCDTAWHGYS
KPIASVLPTSNIYYNPNLKPYEFNLNRAAEILDQAGYKLGPNGVRASSNGVPLSYTILVPSKFPEAVNAAQVIANWWKQI
GVNVEIKTMDTGSMSAIIWNNVNGVVRLGHDIDIWDWIVSPNDVSQFDVFATGRTLTGVSDSGYSNPEYDSLYEKIYNAS
SMDEIKNIAWMLQEILYNDLPYIPLCEIEAVQAHSVKFTGFDYGWPGGPFGGDDWRTFLNTTPVATTTAQGGNSDYLVIG
VAAGVIAVVAIVVLINRFRR
>Mature_580_residues
MNARGTIVKAVAVIVALLLVAPSVSTVSTAQSQSVFRVGWGGTSLDTFNPFTTYAQISTWITLDIYSRLVRVSSNYSTYI
PDLAASWEVIGGDTVRFHLVTNATFHDGVPVTASDVEYSFHLASEPWSSRAANVKIVESIRVIDNYTIDFTVSSIPLFFA
LAASNIPIVPKHIWSNISDPSTYSGYPPIGSGPLRLTEYKEGQYVVLEPYNRFYYPWYLPRVDRIIVKFYPDVTSAANAL
IAGDIDAVGPYIPMAMRNVLVNNPGFKVFTSPGTTYFYIAFNVDPNGTGNPTLKDLNVRRALAYATNVTYVCDTAWHGYS
KPIASVLPTSNIYYNPNLKPYEFNLNRAAEILDQAGYKLGPNGVRASSNGVPLSYTILVPSKFPEAVNAAQVIANWWKQI
GVNVEIKTMDTGSMSAIIWNNVNGVVRLGHDIDIWDWIVSPNDVSQFDVFATGRTLTGVSDSGYSNPEYDSLYEKIYNAS
SMDEIKNIAWMLQEILYNDLPYIPLCEIEAVQAHSVKFTGFDYGWPGGPFGGDDWRTFLNTTPVATTTAQGGNSDYLVIG
VAAGVIAVVAIVVLINRFRR

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=512, Percent_Identity=23.6328125, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1787052, Length=531, Percent_Identity=24.105461393597, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789966, Length=459, Percent_Identity=23.5294117647059, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787762, Length=489, Percent_Identity=24.1308793456033, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789887, Length=374, Percent_Identity=26.7379679144385, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081878, Length=478, Percent_Identity=23.6401673640167, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 63852; Mature: 63852

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARGTIVKAVAVIVALLLVAPSVSTVSTAQSQSVFRVGWGGTSLDTFNPFTTYAQISTW
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCHHEEEHEEE
ITLDIYSRLVRVSSNYSTYIPDLAASWEVIGGDTVRFHLVTNATFHDGVPVTASDVEYSF
EEHHHHHHHHHHCCCCCEECCCCCCCCEEECCCEEEEEEEECCEECCCCCEEECCCEEEE
HLASEPWSSRAANVKIVESIRVIDNYTIDFTVSSIPLFFALAASNIPIVPKHIWSNISDP
EECCCCCCCCCCCEEEEEEEEEEECEEEEEEECCHHEEEHHHHCCCCEECHHHHCCCCCC
STYSGYPPIGSGPLRLTEYKEGQYVVLEPYNRFYYPWYLPRVDRIIVKFYPDVTSAANAL
CCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEECEEECCCHHEEEEEECCCHHHHCCEE
IAGDIDAVGPYIPMAMRNVLVNNPGFKVFTSPGTTYFYIAFNVDPNGTGNPTLKDLNVRR
EECCCHHCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCCCCCEECCHHHH
ALAYATNVTYVCDTAWHGYSKPIASVLPTSNIYYNPNLKPYEFNLNRAAEILDQAGYKLG
HHHHHCCCEEEEECCCCCCCCCHHHHCCCCCEEECCCCCEEEECHHHHHHHHHHCCCEEC
PNGVRASSNGVPLSYTILVPSKFPEAVNAAQVIANWWKQIGVNVEIKTMDTGSMSAIIWN
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEE
NVNGVVRLGHDIDIWDWIVSPNDVSQFDVFATGRTLTGVSDSGYSNPEYDSLYEKIYNAS
CCCCEEEECCCCEEEEEEECCCCCCEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHCCC
SMDEIKNIAWMLQEILYNDLPYIPLCEIEAVQAHSVKFTGFDYGWPGGPFGGDDWRTFLN
CHHHHHHHHHHHHHHHHCCCCCCEEECEEEEEECEEEEECCCCCCCCCCCCCCHHHHHHC
TTPVATTTAQGGNSDYLVIGVAAGVIAVVAIVVLINRFRR
CCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNARGTIVKAVAVIVALLLVAPSVSTVSTAQSQSVFRVGWGGTSLDTFNPFTTYAQISTW
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCHHEEEHEEE
ITLDIYSRLVRVSSNYSTYIPDLAASWEVIGGDTVRFHLVTNATFHDGVPVTASDVEYSF
EEHHHHHHHHHHCCCCCEECCCCCCCCEEECCCEEEEEEEECCEECCCCCEEECCCEEEE
HLASEPWSSRAANVKIVESIRVIDNYTIDFTVSSIPLFFALAASNIPIVPKHIWSNISDP
EECCCCCCCCCCCEEEEEEEEEEECEEEEEEECCHHEEEHHHHCCCCEECHHHHCCCCCC
STYSGYPPIGSGPLRLTEYKEGQYVVLEPYNRFYYPWYLPRVDRIIVKFYPDVTSAANAL
CCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEECEEECCCHHEEEEEECCCHHHHCCEE
IAGDIDAVGPYIPMAMRNVLVNNPGFKVFTSPGTTYFYIAFNVDPNGTGNPTLKDLNVRR
EECCCHHCCCHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCCCCCEECCHHHH
ALAYATNVTYVCDTAWHGYSKPIASVLPTSNIYYNPNLKPYEFNLNRAAEILDQAGYKLG
HHHHHCCCEEEEECCCCCCCCCHHHHCCCCCEEECCCCCEEEECHHHHHHHHHHCCCEEC
PNGVRASSNGVPLSYTILVPSKFPEAVNAAQVIANWWKQIGVNVEIKTMDTGSMSAIIWN
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEEE
NVNGVVRLGHDIDIWDWIVSPNDVSQFDVFATGRTLTGVSDSGYSNPEYDSLYEKIYNAS
CCCCEEEECCCCEEEEEEECCCCCCEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHCCC
SMDEIKNIAWMLQEILYNDLPYIPLCEIEAVQAHSVKFTGFDYGWPGGPFGGDDWRTFLN
CHHHHHHHHHHHHHHHHCCCCCCEEECEEEEEECEEEEECCCCCCCCCCCCCCHHHHHHC
TTPVATTTAQGGNSDYLVIGVAAGVIAVVAIVVLINRFRR
CCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]