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The map label for this gene is appB [H]

Identifier: 218884177

GI number: 218884177

Start: 831060

End: 832034

Strand: Reverse

Name: appB [H]

Synonym: DKAM_0866

Alternate gene names: 218884177

Gene position: 832034-831060 (Counterclockwise)

Preceding gene: 218884178

Following gene: 218884176

Centisome position: 60.94

GC content: 45.54

Gene sequence:

>975_bases
GTGATACCTGTGAACACCACGTTCCTTACTAGAAGGGCTTTTTCAAGCGTTCTCTCCATCTTCATATTACTATCTATATC
ATTCATTCTTTTCAGGCTTCTCCCAGGCGACCCTATTTCCCTGATGTTCAGGGATCCAAGGTTGACCCAGGATCAATTAG
CTTACCTCAGGGAGAAATTCGGGCTCAATAAGCCGCTGTGGGAGCAGTACTTCATTTACTTATGGAATACCGTGAGAGGA
GATCTAGGCATCTCATTCTACTATAAGCAACCAGTAACTGAGATACTATTAGAGAGGATCGTGAACACACTGATGCTCCT
TATACCTGCAACAATAGTGTCTATACTGCTTGGAGTACTAACCGGCGTGGTATCAGCTTGGAGGCATGGTAGTTTAGTTG
ACAAAGTAGTCAGCGGATCGGCGCTAGTATTATACACTCTTCCAACCTACTGGCTTGGAGGATTACTAATACTGGCCTCA
ATAAACTACCTTCATCTCCCAGTTACCGGTATGCGTGACTATGGCGTACACTACTCCTCAATGCTTGAACACCTATACGA
CCTGGCATCCCACATGATACTACCATTCATAACCCTTGTCCTCGTGAACTACGGTGGCTTCACATTGCTAGTCAGGGCAT
CAATGCTTGACGTGTTGGGCGAGGACTATATCAGGACATACTATGCAGTTGGCTTCAGGGAGGGAAGAATACTCTTCAAG
TACGGGTTGAAGAATGCTATGCTTCCAACTCTCACAGCTATATCTATAGCGATAGCTACCTCGGTTTCAGGTGCTGTACT
CACCGAGACTATTTTCGCATGGCCAGGCATAGGAAGGCTAATATATGAAGCCGTATATAATAGGGACTACCCGCTTCTCC
AAGGAGCCTTCCTCGTAATAGCCGTCTCAGTTGTACTAGCCAACCTCATAGCAGACCTATTATATGGATTGATTGATCCA
AGGGTGAGAACATGA

Upstream 100 bases:

>100_bases
CTACAGCCCAGGGTGGCAACAGCGACTACCTAGTGATAGGTGTAGCAGCAGGAGTTATCGCAGTGGTTGCAATAGTGGTC
TTAATTAATAGATTCAGGAG

Downstream 100 bases:

>100_bases
CCACGGGTGGAAGCAGTAACAGGTTCGCTACTAGTTTAACAGTATTAGCGAAAGACATGCTATGGGAAATAGTATCGATC
ACCAGGAGGAATAGTAGTGT

Product: dipeptide/oligopeptide/nickel ABC transporter permease

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MIPVNTTFLTRRAFSSVLSIFILLSISFILFRLLPGDPISLMFRDPRLTQDQLAYLREKFGLNKPLWEQYFIYLWNTVRG
DLGISFYYKQPVTEILLERIVNTLMLLIPATIVSILLGVLTGVVSAWRHGSLVDKVVSGSALVLYTLPTYWLGGLLILAS
INYLHLPVTGMRDYGVHYSSMLEHLYDLASHMILPFITLVLVNYGGFTLLVRASMLDVLGEDYIRTYYAVGFREGRILFK
YGLKNAMLPTLTAISIAIATSVSGAVLTETIFAWPGIGRLIYEAVYNRDYPLLQGAFLVIAVSVVLANLIADLLYGLIDP
RVRT

Sequences:

>Translated_324_residues
MIPVNTTFLTRRAFSSVLSIFILLSISFILFRLLPGDPISLMFRDPRLTQDQLAYLREKFGLNKPLWEQYFIYLWNTVRG
DLGISFYYKQPVTEILLERIVNTLMLLIPATIVSILLGVLTGVVSAWRHGSLVDKVVSGSALVLYTLPTYWLGGLLILAS
INYLHLPVTGMRDYGVHYSSMLEHLYDLASHMILPFITLVLVNYGGFTLLVRASMLDVLGEDYIRTYYAVGFREGRILFK
YGLKNAMLPTLTAISIAIATSVSGAVLTETIFAWPGIGRLIYEAVYNRDYPLLQGAFLVIAVSVVLANLIADLLYGLIDP
RVRT
>Mature_324_residues
MIPVNTTFLTRRAFSSVLSIFILLSISFILFRLLPGDPISLMFRDPRLTQDQLAYLREKFGLNKPLWEQYFIYLWNTVRG
DLGISFYYKQPVTEILLERIVNTLMLLIPATIVSILLGVLTGVVSAWRHGSLVDKVVSGSALVLYTLPTYWLGGLLILAS
INYLHLPVTGMRDYGVHYSSMLEHLYDLASHMILPFITLVLVNYGGFTLLVRASMLDVLGEDYIRTYYAVGFREGRILFK
YGLKNAMLPTLTAISIAIATSVSGAVLTETIFAWPGIGRLIYEAVYNRDYPLLQGAFLVIAVSVVLANLIADLLYGLIDP
RVRT

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=334, Percent_Identity=38.3233532934132, Blast_Score=230, Evalue=9e-62,
Organism=Escherichia coli, GI1787053, Length=319, Percent_Identity=31.6614420062696, Blast_Score=177, Evalue=6e-46,
Organism=Escherichia coli, GI1789888, Length=301, Percent_Identity=34.8837209302326, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787497, Length=318, Percent_Identity=32.0754716981132, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787761, Length=332, Percent_Identity=34.0361445783133, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1788504, Length=279, Percent_Identity=28.673835125448, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787550, Length=272, Percent_Identity=27.2058823529412, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36387; Mature: 36387

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVNTTFLTRRAFSSVLSIFILLSISFILFRLLPGDPISLMFRDPRLTQDQLAYLREKF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
GLNKPLWEQYFIYLWNTVRGDLGISFYYKQPVTEILLERIVNTLMLLIPATIVSILLGVL
CCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVVSAWRHGSLVDKVVSGSALVLYTLPTYWLGGLLILASINYLHLPVTGMRDYGVHYSS
HHHHHHHHCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCEEECCCCCHHHCCCHHH
MLEHLYDLASHMILPFITLVLVNYGGFTLLVRASMLDVLGEDYIRTYYAVGFREGRILFK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
YGLKNAMLPTLTAISIAIATSVSGAVLTETIFAWPGIGRLIYEAVYNRDYPLLQGAFLVI
ECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
AVSVVLANLIADLLYGLIDPRVRT
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIPVNTTFLTRRAFSSVLSIFILLSISFILFRLLPGDPISLMFRDPRLTQDQLAYLREKF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
GLNKPLWEQYFIYLWNTVRGDLGISFYYKQPVTEILLERIVNTLMLLIPATIVSILLGVL
CCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVVSAWRHGSLVDKVVSGSALVLYTLPTYWLGGLLILASINYLHLPVTGMRDYGVHYSS
HHHHHHHHCCHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCEEECCCCCHHHCCCHHH
MLEHLYDLASHMILPFITLVLVNYGGFTLLVRASMLDVLGEDYIRTYYAVGFREGRILFK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
YGLKNAMLPTLTAISIAIATSVSGAVLTETIFAWPGIGRLIYEAVYNRDYPLLQGAFLVI
ECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
AVSVVLANLIADLLYGLIDPRVRT
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]