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The map label for this gene is ppsA [H]

Identifier: 218883942

GI number: 218883942

Start: 602736

End: 605228

Strand: Direct

Name: ppsA [H]

Synonym: DKAM_0631

Alternate gene names: 218883942

Gene position: 602736-605228 (Clockwise)

Preceding gene: 218883941

Following gene: 218883943

Centisome position: 44.15

GC content: 46.13

Gene sequence:

>2493_bases
ATGAATAAGGAGAACAAGTTCGTTCTATGGCTTGAGGAAGTTAGAAAAGAAGATGTCCCATTGGTTGGAGGTAAGAATGC
AAACCTTGGAGAAATGATTAATGCTGGGATCCCAGTACCACCTGGATTTGCTGTTACAGCATACGCATTCAAGTACTTCC
TTGAGAAGACGGGGCTTGGCGAGAAAATATATGGCATGCTGAGGAAGCTCGATGTAAACAATACTAAGGAACTTGAGGAG
ACAACCGCGAAAATAAGAGAAATGATAATGAACCAGCCTATGCCTCCAGAAGTAGAGGAGGAGATAAAGAAGTACTATAT
CGAGCTGGCAAAGAAGCTAGGTATGGATCCAAACAAGCTTAGAGTAGCTGTTAGAAGTAGTGCTACAGCAGAGGACCTGC
CAGAGGCTAGTTTCGCTGGACAACAGGACACCTATCTAAACGTGTATGGAGCCGACAGCGTAGTATACCATGTTAAGAGA
TGCTGGGCAAGCCTCTTCACCGCAAGAGCAACATTCTACCGTGTTGCCCAGGGCATTCCACACGAGAAAACATTCATGAG
TGTAACGGTTCAGAAGATGGTTAACAGCAGAAGTGCAGGCGTAATGTTCACACTTCACCCTGTAACAGGAGATGAGAATG
TCGTAGTGATAGAGGGTAGCTGGGGTCTAGGTGAGTCAGTCGTAGGCGGTAAGGTTACTCCCGACGAGTTCGTTGTGGAT
AAGAAGACACTTAGCATAGTGGAAAAGCACATCAATAGAAAGATATTCATGATAACATTCGATCCAGCTCTAGGTAAAAA
CGTCCACTTGAAATGGGATGATCAGAAAGGTAGGTGGGTTGCTGAGGAAGGCGAAAACATTAGCCCGCCCCTAGCTCAGG
TAGCCAATCCAGATAAGCAGGCATTGACAGAGGAAGAGGTTAGGAAGCTTGCTGAGCTGGCATTATTGATCCAGAGGCAC
TATGGTAAACACATGGATATTGAGTGGGCAATCGACTCTGACCTACAGTTCCCAGCCAACGTATTTATTGTGCAGGCAAG
ACCAGAGACGGTTTGGAGTACCAGGAAGGCTAAGGAAGCCGAAAAGAAACCAGAGGAGGTAAAGGGTAAAACCGTGAAGC
TAGGTGAAGCAAAAGTACTAATTAGGGGGCTGCCAGCTTCACCAGGTATTGGTGCTGGTGTTGCTAAAGTCATATTCGAC
CCGAAGAGCAAGGAGGCCATGGAGTTCAAGGAAGGTGACATACTGGTAACAAAGATGACTGATCCTGATTGGGTACCATT
AATGAAGAAAGCTAGAGCAATAGTAACCGATGAGGGAGGTATGACAAGTCACGCTGCAATAGTTAGCCGTGAGCTGGGTA
TTCCAGCAGTCGTTGGAACAGGCAATGCCACACAGGTCATTAAATCTGGTGTGGAGGTAACTGTTGATGGTGGCAGGGGA
GTAATATATGAGGGTATAGTTGAGGAACTAGTAAAGCCAAAAGCTGAGGCGAAGACCGAGGGAGTAGTTGCAGCAGTAGG
TATCAGCCCTGAGCAACTACTGCCATTATACCCTGTCACGGCTACAAGGATATACATGAACCTCGGTGAGCCAGACGCTA
TTGAGAAATACAAGGATCTACCCTTCGATGGCATAGGCCTAATGAGGATAGAGTTCATATTCACTGACTGGGTACAGTAT
CACCCATTATATCTAATTGAGATAGGTAAGCCGGAAATAGTCGTCAACAAGCTGGCTGAGGGTATAGCTAAGGTTGCACA
GGCAATATATCCAAGGCCAGTTGTAGTGAGATTCAGCGACTTCAAGACAAATGAGTACCGTGGATTGAAAGGCGGCGAGA
AATATGAGCCTGAGGAGAGGAACCCGATGATAGGATGGCGTGGAGTCAGCAGGTACATTCACCCGAAGTATGAGCCAGGC
TTCAGGTTAGAGGTTAGAGCCATTAGGAAAGTAAGAGAAGAAATGGGATTAAGCAACGTATGGGTAATGTTCCCATTTGT
CAGGACAACGTGGGAGCTTGATAAAGCCATTAAGATAATGGAGGAGGAGGGATTAAAGAGGAGTAAGGACTTCAAGGTAT
GGGCAATGGCTGAGGTACCAAGCATAGCACTACTGGCTGATAAGTTCGCTGAGTACGTTGATGGATTTAGTATAGGTAGC
AACGACCTCACGCAGTTGGTGCTTGGTGCAGACAGAGACAGCAATATACTCGCTGAAATGGGCTACTTTGATGAGAGAGA
TCCAGCTGTACTAGAAGCGATTAGAATGATAATTGAGAAGGCTCATAGTAAGGGAGCTACTGTAAGCATTTGTGGGCAAG
CTCCAAGCGTATACCCAGAGATAGTGGAGTTCCTGGTAAGGCTAGGAATAGACAGCATAAGTGTCAACCCAGACGCAGTG
ATATCAACAAGGAGGCTAGTGGCAAGCGTTGAGAGAAAGATCATGTTAGAGAGGTTGAATAAGATAATTGAGAAGCTGAA
GGACTTCGAGTAA

Upstream 100 bases:

>100_bases
TTTCCTATGCATCATTGACTATATACTATTAATAGATGTATATAGGAAAGCTTTTTATCCCTCATAAGTATTCTTTGAAG
ACAAGCGAAAAGGTGGTTAA

Downstream 100 bases:

>100_bases
TTTTTCCCTAAAAATCATTTTTAATCCTTTAACCCGTTCAGCCAGCGATCAGCACTGGTAATATTAATCTAAATATGTTT
TCCACAAACGTTTATATAGT

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 830; Mature: 830

Protein sequence:

>830_residues
MNKENKFVLWLEEVRKEDVPLVGGKNANLGEMINAGIPVPPGFAVTAYAFKYFLEKTGLGEKIYGMLRKLDVNNTKELEE
TTAKIREMIMNQPMPPEVEEEIKKYYIELAKKLGMDPNKLRVAVRSSATAEDLPEASFAGQQDTYLNVYGADSVVYHVKR
CWASLFTARATFYRVAQGIPHEKTFMSVTVQKMVNSRSAGVMFTLHPVTGDENVVVIEGSWGLGESVVGGKVTPDEFVVD
KKTLSIVEKHINRKIFMITFDPALGKNVHLKWDDQKGRWVAEEGENISPPLAQVANPDKQALTEEEVRKLAELALLIQRH
YGKHMDIEWAIDSDLQFPANVFIVQARPETVWSTRKAKEAEKKPEEVKGKTVKLGEAKVLIRGLPASPGIGAGVAKVIFD
PKSKEAMEFKEGDILVTKMTDPDWVPLMKKARAIVTDEGGMTSHAAIVSRELGIPAVVGTGNATQVIKSGVEVTVDGGRG
VIYEGIVEELVKPKAEAKTEGVVAAVGISPEQLLPLYPVTATRIYMNLGEPDAIEKYKDLPFDGIGLMRIEFIFTDWVQY
HPLYLIEIGKPEIVVNKLAEGIAKVAQAIYPRPVVVRFSDFKTNEYRGLKGGEKYEPEERNPMIGWRGVSRYIHPKYEPG
FRLEVRAIRKVREEMGLSNVWVMFPFVRTTWELDKAIKIMEEEGLKRSKDFKVWAMAEVPSIALLADKFAEYVDGFSIGS
NDLTQLVLGADRDSNILAEMGYFDERDPAVLEAIRMIIEKAHSKGATVSICGQAPSVYPEIVEFLVRLGIDSISVNPDAV
ISTRRLVASVERKIMLERLNKIIEKLKDFE

Sequences:

>Translated_830_residues
MNKENKFVLWLEEVRKEDVPLVGGKNANLGEMINAGIPVPPGFAVTAYAFKYFLEKTGLGEKIYGMLRKLDVNNTKELEE
TTAKIREMIMNQPMPPEVEEEIKKYYIELAKKLGMDPNKLRVAVRSSATAEDLPEASFAGQQDTYLNVYGADSVVYHVKR
CWASLFTARATFYRVAQGIPHEKTFMSVTVQKMVNSRSAGVMFTLHPVTGDENVVVIEGSWGLGESVVGGKVTPDEFVVD
KKTLSIVEKHINRKIFMITFDPALGKNVHLKWDDQKGRWVAEEGENISPPLAQVANPDKQALTEEEVRKLAELALLIQRH
YGKHMDIEWAIDSDLQFPANVFIVQARPETVWSTRKAKEAEKKPEEVKGKTVKLGEAKVLIRGLPASPGIGAGVAKVIFD
PKSKEAMEFKEGDILVTKMTDPDWVPLMKKARAIVTDEGGMTSHAAIVSRELGIPAVVGTGNATQVIKSGVEVTVDGGRG
VIYEGIVEELVKPKAEAKTEGVVAAVGISPEQLLPLYPVTATRIYMNLGEPDAIEKYKDLPFDGIGLMRIEFIFTDWVQY
HPLYLIEIGKPEIVVNKLAEGIAKVAQAIYPRPVVVRFSDFKTNEYRGLKGGEKYEPEERNPMIGWRGVSRYIHPKYEPG
FRLEVRAIRKVREEMGLSNVWVMFPFVRTTWELDKAIKIMEEEGLKRSKDFKVWAMAEVPSIALLADKFAEYVDGFSIGS
NDLTQLVLGADRDSNILAEMGYFDERDPAVLEAIRMIIEKAHSKGATVSICGQAPSVYPEIVEFLVRLGIDSISVNPDAV
ISTRRLVASVERKIMLERLNKIIEKLKDFE
>Mature_830_residues
MNKENKFVLWLEEVRKEDVPLVGGKNANLGEMINAGIPVPPGFAVTAYAFKYFLEKTGLGEKIYGMLRKLDVNNTKELEE
TTAKIREMIMNQPMPPEVEEEIKKYYIELAKKLGMDPNKLRVAVRSSATAEDLPEASFAGQQDTYLNVYGADSVVYHVKR
CWASLFTARATFYRVAQGIPHEKTFMSVTVQKMVNSRSAGVMFTLHPVTGDENVVVIEGSWGLGESVVGGKVTPDEFVVD
KKTLSIVEKHINRKIFMITFDPALGKNVHLKWDDQKGRWVAEEGENISPPLAQVANPDKQALTEEEVRKLAELALLIQRH
YGKHMDIEWAIDSDLQFPANVFIVQARPETVWSTRKAKEAEKKPEEVKGKTVKLGEAKVLIRGLPASPGIGAGVAKVIFD
PKSKEAMEFKEGDILVTKMTDPDWVPLMKKARAIVTDEGGMTSHAAIVSRELGIPAVVGTGNATQVIKSGVEVTVDGGRG
VIYEGIVEELVKPKAEAKTEGVVAAVGISPEQLLPLYPVTATRIYMNLGEPDAIEKYKDLPFDGIGLMRIEFIFTDWVQY
HPLYLIEIGKPEIVVNKLAEGIAKVAQAIYPRPVVVRFSDFKTNEYRGLKGGEKYEPEERNPMIGWRGVSRYIHPKYEPG
FRLEVRAIRKVREEMGLSNVWVMFPFVRTTWELDKAIKIMEEEGLKRSKDFKVWAMAEVPSIALLADKFAEYVDGFSIGS
NDLTQLVLGADRDSNILAEMGYFDERDPAVLEAIRMIIEKAHSKGATVSICGQAPSVYPEIVEFLVRLGIDSISVNPDAV
ISTRRLVASVERKIMLERLNKIIEKLKDFE

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=836, Percent_Identity=43.0622009569378, Blast_Score=623, Evalue=1e-179,
Organism=Escherichia coli, GI1788756, Length=394, Percent_Identity=26.1421319796954, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1789193, Length=405, Percent_Identity=27.4074074074074, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI1788726, Length=396, Percent_Identity=24.7474747474747, Blast_Score=106, Evalue=7e-24,
Organism=Escherichia coli, GI48994992, Length=388, Percent_Identity=24.4845360824742, Blast_Score=97, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17564524, Length=236, Percent_Identity=34.7457627118644, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 92730; Mature: 92730

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKENKFVLWLEEVRKEDVPLVGGKNANLGEMINAGIPVPPGFAVTAYAFKYFLEKTGLG
CCCCCCEEEEHHHHHHCCCCEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
EKIYGMLRKLDVNNTKELEETTAKIREMIMNQPMPPEVEEEIKKYYIELAKKLGMDPNKL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHE
RVAVRSSATAEDLPEASFAGQQDTYLNVYGADSVVYHVKRCWASLFTARATFYRVAQGIP
EEEEECCCCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HEKTFMSVTVQKMVNSRSAGVMFTLHPVTGDENVVVIEGSWGLGESVVGGKVTPDEFVVD
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHEEC
KKTLSIVEKHINRKIFMITFDPALGKNVHLKWDDQKGRWVAEEGENISPPLAQVANPDKQ
HHHHHHHHHHHCCEEEEEEECCCCCCEEEEEECCCCCCEECCCCCCCCCCHHHHCCCCHH
ALTEEEVRKLAELALLIQRHYGKHMDIEWAIDSDLQFPANVFIVQARPETVWSTRKAKEA
HCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHH
EKKPEEVKGKTVKLGEAKVLIRGLPASPGIGAGVAKVIFDPKSKEAMEFKEGDILVTKMT
HCCHHHHCCCEEEECCEEEEEEECCCCCCCCCCHHHEEECCCCCHHHHCCCCCEEEEECC
DPDWVPLMKKARAIVTDEGGMTSHAAIVSRELGIPAVVGTGNATQVIKSGVEVTVDGGRG
CCCHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHCCCEEEEECCCC
VIYEGIVEELVKPKAEAKTEGVVAAVGISPEQLLPLYPVTATRIYMNLGEPDAIEKYKDL
EEHHHHHHHHHCCCHHCCCCCEEEEECCCHHHCCCCCCCEEEEEEEECCCCHHHHHHHCC
PFDGIGLMRIEFIFTDWVQYHPLYLIEIGKPEIVVNKLAEGIAKVAQAIYPRPVVVRFSD
CCCCCCCEEEEHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
FKTNEYRGLKGGEKYEPEERNPMIGWRGVSRYIHPKYEPGFRLEVRAIRKVREEMGLSNV
CCCCCCCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCCEEHHHHHHHHHHHCCCCCE
WVMFPFVRTTWELDKAIKIMEEEGLKRSKDFKVWAMAEVPSIALLADKFAEYVDGFSIGS
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC
NDLTQLVLGADRDSNILAEMGYFDERDPAVLEAIRMIIEKAHSKGATVSICGQAPSVYPE
HHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
IVEFLVRLGIDSISVNPDAVISTRRLVASVERKIMLERLNKIIEKLKDFE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKENKFVLWLEEVRKEDVPLVGGKNANLGEMINAGIPVPPGFAVTAYAFKYFLEKTGLG
CCCCCCEEEEHHHHHHCCCCEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
EKIYGMLRKLDVNNTKELEETTAKIREMIMNQPMPPEVEEEIKKYYIELAKKLGMDPNKL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHE
RVAVRSSATAEDLPEASFAGQQDTYLNVYGADSVVYHVKRCWASLFTARATFYRVAQGIP
EEEEECCCCHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HEKTFMSVTVQKMVNSRSAGVMFTLHPVTGDENVVVIEGSWGLGESVVGGKVTPDEFVVD
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHEEC
KKTLSIVEKHINRKIFMITFDPALGKNVHLKWDDQKGRWVAEEGENISPPLAQVANPDKQ
HHHHHHHHHHHCCEEEEEEECCCCCCEEEEEECCCCCCEECCCCCCCCCCHHHHCCCCHH
ALTEEEVRKLAELALLIQRHYGKHMDIEWAIDSDLQFPANVFIVQARPETVWSTRKAKEA
HCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHH
EKKPEEVKGKTVKLGEAKVLIRGLPASPGIGAGVAKVIFDPKSKEAMEFKEGDILVTKMT
HCCHHHHCCCEEEECCEEEEEEECCCCCCCCCCHHHEEECCCCCHHHHCCCCCEEEEECC
DPDWVPLMKKARAIVTDEGGMTSHAAIVSRELGIPAVVGTGNATQVIKSGVEVTVDGGRG
CCCHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHCCCEEEEECCCC
VIYEGIVEELVKPKAEAKTEGVVAAVGISPEQLLPLYPVTATRIYMNLGEPDAIEKYKDL
EEHHHHHHHHHCCCHHCCCCCEEEEECCCHHHCCCCCCCEEEEEEEECCCCHHHHHHHCC
PFDGIGLMRIEFIFTDWVQYHPLYLIEIGKPEIVVNKLAEGIAKVAQAIYPRPVVVRFSD
CCCCCCCEEEEHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
FKTNEYRGLKGGEKYEPEERNPMIGWRGVSRYIHPKYEPGFRLEVRAIRKVREEMGLSNV
CCCCCCCCCCCCCCCCCCCCCCCEEECCHHHCCCCCCCCCCEEHHHHHHHHHHHCCCCCE
WVMFPFVRTTWELDKAIKIMEEEGLKRSKDFKVWAMAEVPSIALLADKFAEYVDGFSIGS
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCC
NDLTQLVLGADRDSNILAEMGYFDERDPAVLEAIRMIIEKAHSKGATVSICGQAPSVYPE
HHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
IVEFLVRLGIDSISVNPDAVISTRRLVASVERKIMLERLNKIIEKLKDFE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7805870 [H]