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The map label for this gene is pycA [H]

Identifier: 218883941

GI number: 218883941

Start: 601070

End: 602578

Strand: Direct

Name: pycA [H]

Synonym: DKAM_0630

Alternate gene names: 218883941

Gene position: 601070-602578 (Clockwise)

Preceding gene: 218883940

Following gene: 218883942

Centisome position: 44.03

GC content: 44.86

Gene sequence:

>1509_bases
ATGTCTAGGAGGATACTTATTGCTAATAAGGGAGAGGTGGCTGTAAGGATAGCCCGTACCCTTATTGAAAAAGGATACCA
CCCCTTAGGAGTATATGTCAGCAACGATGAGTTGAGCCCCCATAGAAGATACATGGTTGAAGAGGCGAAGATATCGAGCT
ACACAAACGCAGAGGAGATTATTGAGGCTGCATTAGAGCTCGGTGCAGAAGCCATACACCCAGGGTACGGCTTACTCGAG
AGCGAACCGGGGTTTTCCCAGGAGGTTGCACGCAGGGGATTAATATTCGTTGGCCCACCGCCTGGTCAAATAGAATTTGC
TCGTGATAAACTAGCTGTCAAAACACTGGCGGAGAAGCTTGAAATACCGACCCTTCCATGGAGCCTTGTGAAAAACGAGG
AGGATCTCGAGGAATTCGTAAGAACGTATGGCTATCCATTAATACTTAAACCCGTAAAAGGTAACTGGGGTAGGGGGCTT
AGGATACTGTGGAGCGAGAAGGATCGAGACCAATTGAAGACCCTTATCAAGGAAGCCGAGAAAATATACAGGGATTCAAG
GATATATGTCGAGCCATATATAACAAACGTTAAACACATAGAGATACAATTAATTGGTGACGGGGAGAAAGTAATTCATT
TATATGATAGGGAATGCAGTATCCAGGATAACTACATGAAAATCCTTGTGGAGGCTCCCTCCACACTCCAAGTTAATGGT
TTACGGGAAAAAATACTCAATTACGCCTTGTCAATCGGGGAAGCTATGAGGCTGAGAAATCTTGCAACAATAGAGTTTCT
ATATGATGCAAGAAGTCGCAGTGTCTTCCTTAATGAAGTAAACATGGGTCTATCCCCTGAGCACGCTGTCACAGAGGCTG
TCACTAGGATTGACCTAGTTGAGAAGCAGGTTGAGGCGGCCCTATATTCTACAATAGGATTGAAGCAAGAAAACGTCTCA
TTGAATGGGTGGGCTTTCCAGGCCAAGGTATTTAATAAAAACATATTCAGGGATGAGCCTTCAAGCGGCTTAATTAAGAC
TTATAACGAGCCCAACGGCTTAGGAGTAGCATGTGACACTGGGATAGCTCCAGGAGTTAAGGTAAGAGACGAGTACACCC
TCTTGGTTAAGGTGCTTGCCTGGGGGAGCAGTAGGCTTATAGCTTTAAATAGGCTGAAGCGTGCCTTAAACGATATATAT
ATCAATGGAGTGCACACCAATATACCAGTGCTAAGGGAGATCCTTGACTCGAAGGATGTTGAAAACGGGATCTACAACAC
CAGATTCCTCGTGGAGAAGAGGGATGAAATTATCTCTAAGCTCAGAGAAAGAATGCTGCTCCACGCTATCGCATCTATCT
CCCTGATCGAGTACGGTAGTAAGGAGGCAAGGAAAATACTTGGAGAAGGCAAGTTGAGTACTGAGAAGATAATGGAGAAT
GCTTCAAGAACAAAGAGGACAGCATGGTTCTACTACGCCAGGCTAAGGGAGAGGATTAGGAGAAAATAA

Upstream 100 bases:

>100_bases
CACAATCAGTCCTAGGCAAACATACAACAACCTAGGACTGGAAATGGTTGGTTTTATTAAGTATGTGAGAATAATGTAGG
TTAAGATAAGGAGGTCAGTG

Downstream 100 bases:

>100_bases
GGTTGGAGAGGCCTTCCGCCGATGCACGTGTTTTCAACACGTCTTAGATATCAATAGTTTCCTATGCATCATTGACTATA
TACTATTAATAGATGTATAT

Product: carbamoyl-phosphate synthase L chain, ATP-binding

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSRRILIANKGEVAVRIARTLIEKGYHPLGVYVSNDELSPHRRYMVEEAKISSYTNAEEIIEAALELGAEAIHPGYGLLE
SEPGFSQEVARRGLIFVGPPPGQIEFARDKLAVKTLAEKLEIPTLPWSLVKNEEDLEEFVRTYGYPLILKPVKGNWGRGL
RILWSEKDRDQLKTLIKEAEKIYRDSRIYVEPYITNVKHIEIQLIGDGEKVIHLYDRECSIQDNYMKILVEAPSTLQVNG
LREKILNYALSIGEAMRLRNLATIEFLYDARSRSVFLNEVNMGLSPEHAVTEAVTRIDLVEKQVEAALYSTIGLKQENVS
LNGWAFQAKVFNKNIFRDEPSSGLIKTYNEPNGLGVACDTGIAPGVKVRDEYTLLVKVLAWGSSRLIALNRLKRALNDIY
INGVHTNIPVLREILDSKDVENGIYNTRFLVEKRDEIISKLRERMLLHAIASISLIEYGSKEARKILGEGKLSTEKIMEN
ASRTKRTAWFYYARLRERIRRK

Sequences:

>Translated_502_residues
MSRRILIANKGEVAVRIARTLIEKGYHPLGVYVSNDELSPHRRYMVEEAKISSYTNAEEIIEAALELGAEAIHPGYGLLE
SEPGFSQEVARRGLIFVGPPPGQIEFARDKLAVKTLAEKLEIPTLPWSLVKNEEDLEEFVRTYGYPLILKPVKGNWGRGL
RILWSEKDRDQLKTLIKEAEKIYRDSRIYVEPYITNVKHIEIQLIGDGEKVIHLYDRECSIQDNYMKILVEAPSTLQVNG
LREKILNYALSIGEAMRLRNLATIEFLYDARSRSVFLNEVNMGLSPEHAVTEAVTRIDLVEKQVEAALYSTIGLKQENVS
LNGWAFQAKVFNKNIFRDEPSSGLIKTYNEPNGLGVACDTGIAPGVKVRDEYTLLVKVLAWGSSRLIALNRLKRALNDIY
INGVHTNIPVLREILDSKDVENGIYNTRFLVEKRDEIISKLRERMLLHAIASISLIEYGSKEARKILGEGKLSTEKIMEN
ASRTKRTAWFYYARLRERIRRK
>Mature_501_residues
SRRILIANKGEVAVRIARTLIEKGYHPLGVYVSNDELSPHRRYMVEEAKISSYTNAEEIIEAALELGAEAIHPGYGLLES
EPGFSQEVARRGLIFVGPPPGQIEFARDKLAVKTLAEKLEIPTLPWSLVKNEEDLEEFVRTYGYPLILKPVKGNWGRGLR
ILWSEKDRDQLKTLIKEAEKIYRDSRIYVEPYITNVKHIEIQLIGDGEKVIHLYDRECSIQDNYMKILVEAPSTLQVNGL
REKILNYALSIGEAMRLRNLATIEFLYDARSRSVFLNEVNMGLSPEHAVTEAVTRIDLVEKQVEAALYSTIGLKQENVSL
NGWAFQAKVFNKNIFRDEPSSGLIKTYNEPNGLGVACDTGIAPGVKVRDEYTLLVKVLAWGSSRLIALNRLKRALNDIYI
NGVHTNIPVLREILDSKDVENGIYNTRFLVEKRDEIISKLRERMLLHAIASISLIEYGSKEARKILGEGKLSTEKIMENA
SRTKRTAWFYYARLRERIRRK

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI295821183, Length=475, Percent_Identity=34.1052631578947, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI65506442, Length=475, Percent_Identity=34.1052631578947, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI189095269, Length=475, Percent_Identity=34.1052631578947, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI116805327, Length=449, Percent_Identity=31.6258351893096, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI106049528, Length=471, Percent_Identity=30.9978768577495, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI106049295, Length=471, Percent_Identity=30.9978768577495, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI106049292, Length=471, Percent_Identity=30.9978768577495, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI134142062, Length=501, Percent_Identity=23.3532934131737, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI38679960, Length=351, Percent_Identity=25.3561253561254, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI38679977, Length=351, Percent_Identity=25.3561253561254, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI38679967, Length=351, Percent_Identity=25.3561253561254, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI38679974, Length=351, Percent_Identity=25.3561253561254, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI38679971, Length=351, Percent_Identity=25.6410256410256, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1789654, Length=449, Percent_Identity=31.1804008908686, Blast_Score=211, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17567343, Length=444, Percent_Identity=34.009009009009, Blast_Score=241, Evalue=6e-64,
Organism=Caenorhabditis elegans, GI71987519, Length=477, Percent_Identity=31.4465408805031, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17562816, Length=446, Percent_Identity=32.5112107623318, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI71997168, Length=550, Percent_Identity=23.0909090909091, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71997163, Length=543, Percent_Identity=23.3885819521179, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI133931226, Length=540, Percent_Identity=24.0740740740741, Blast_Score=121, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6319695, Length=474, Percent_Identity=31.8565400843882, Blast_Score=227, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6321376, Length=474, Percent_Identity=32.2784810126582, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=428, Percent_Identity=32.2429906542056, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323863, Length=443, Percent_Identity=24.6049661399549, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6324343, Length=515, Percent_Identity=21.747572815534, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24652212, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24652210, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24652214, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI19921944, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24652216, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI281363050, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24652224, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24652222, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24652220, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24652218, Length=447, Percent_Identity=32.2147651006711, Blast_Score=225, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24651757, Length=454, Percent_Identity=32.3788546255507, Blast_Score=217, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24651759, Length=405, Percent_Identity=31.6049382716049, Blast_Score=193, Evalue=2e-49,
Organism=Drosophila melanogaster, GI161076409, Length=346, Percent_Identity=27.1676300578035, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24586458, Length=346, Percent_Identity=27.1676300578035, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161076407, Length=346, Percent_Identity=27.1676300578035, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24586460, Length=346, Percent_Identity=27.1676300578035, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 57215; Mature: 57084

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50975 ATP_GRASP ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRILIANKGEVAVRIARTLIEKGYHPLGVYVSNDELSPHRRYMVEEAKISSYTNAEEI
CCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHH
IEAALELGAEAIHPGYGLLESEPGFSQEVARRGLIFVGPPPGQIEFARDKLAVKTLAEKL
HHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCEEHHHHHHHHHHHHHHH
EIPTLPWSLVKNEEDLEEFVRTYGYPLILKPVKGNWGRGLRILWSEKDRDQLKTLIKEAE
CCCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHH
KIYRDSRIYVEPYITNVKHIEIQLIGDGEKVIHLYDRECSIQDNYMKILVEAPSTLQVNG
HHHCCCEEEEEEEECCEEEEEEEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCEEECH
LREKILNYALSIGEAMRLRNLATIEFLYDARSRSVFLNEVNMGLSPEHAVTEAVTRIDLV
HHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
EKQVEAALYSTIGLKQENVSLNGWAFQAKVFNKNIFRDEPSSGLIKTYNEPNGLGVACDT
HHHHHHHHHHHHCCCCCCCEECCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEECC
GIAPGVKVRDEYTLLVKVLAWGSSRLIALNRLKRALNDIYINGVHTNIPVLREILDSKDV
CCCCCCEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCC
ENGIYNTRFLVEKRDEIISKLRERMLLHAIASISLIEYGSKEARKILGEGKLSTEKIMEN
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHH
ASRTKRTAWFYYARLRERIRRK
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRRILIANKGEVAVRIARTLIEKGYHPLGVYVSNDELSPHRRYMVEEAKISSYTNAEEI
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHH
IEAALELGAEAIHPGYGLLESEPGFSQEVARRGLIFVGPPPGQIEFARDKLAVKTLAEKL
HHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCCEEHHHHHHHHHHHHHHH
EIPTLPWSLVKNEEDLEEFVRTYGYPLILKPVKGNWGRGLRILWSEKDRDQLKTLIKEAE
CCCCCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHH
KIYRDSRIYVEPYITNVKHIEIQLIGDGEKVIHLYDRECSIQDNYMKILVEAPSTLQVNG
HHHCCCEEEEEEEECCEEEEEEEEEECCCEEEEEECCCCCCCCCEEEEEEECCCCEEECH
LREKILNYALSIGEAMRLRNLATIEFLYDARSRSVFLNEVNMGLSPEHAVTEAVTRIDLV
HHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
EKQVEAALYSTIGLKQENVSLNGWAFQAKVFNKNIFRDEPSSGLIKTYNEPNGLGVACDT
HHHHHHHHHHHHCCCCCCCEECCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCEEEECC
GIAPGVKVRDEYTLLVKVLAWGSSRLIALNRLKRALNDIYINGVHTNIPVLREILDSKDV
CCCCCCEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCC
ENGIYNTRFLVEKRDEIISKLRERMLLHAIASISLIEYGSKEARKILGEGKLSTEKIMEN
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHH
ASRTKRTAWFYYARLRERIRRK
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]