Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 218883836

GI number: 218883836

Start: 492463

End: 493962

Strand: Direct

Name: purD [H]

Synonym: DKAM_0525

Alternate gene names: 218883836

Gene position: 492463-493962 (Clockwise)

Preceding gene: 218883835

Following gene: 218883837

Centisome position: 36.07

GC content: 48.0

Gene sequence:

>1500_bases
TTGAAGATCCTGCTAATCGGATCCAGTGCAAGAGAACACGCCCTTGGATTAATGATGTTGAAGAGCGGGTTAGAGCCAAG
GGTGTATGTTGTATCGGAGTACAGGAATCCAGGGCTCGAAAGAGTAGCCGGGTCATCTAGCGGCAGAATATTTATTACCA
GGGTAACAGAGCCGGGTAGGATACTTGAGATCGCTGAGGCCGTGTCACCGGACTTGGTCGTAATAGGTCCCGAGGAACCA
TTATTCGCAGGTATCTCCGATACCCTAAGGGAACACGGATTCGCAGTCTTCGGGGCGTCATCGAAATGTGCTGAGATAGA
GAGGAGCAAGGTTTTCGCGAGGAGGCTCATGTGGAGGCATGGGATCAATGGACGATTATTCTACGCATCCTTCAGTAATG
TGGAGGATGCAGTGAGCTTTGCTGAGCACGCTGGCGATATAGTTGTGAAACCGGCTAGACAGGTCGGTGGAAAGGGGGTT
AAAGTATTCAGTAATACTAAGTCTTATCTATTCAATGAAATAAGCCGTGTCACCGGTGAATACGTTAGGGACTTATTCAG
CGAAATGAATGCGTACAGGGATATCGATGAAAAGATTCTTGTAGAGTTAAGGGTTGAGGGCGTAGAATACACTCTACACG
TAATCTCGAGCGGTGGCTCCATCACGCCATTACCGGTCATACAGGATCATCCCCACGCTTTCGAGTATGATGTAGGGCCT
GAAACAGGTGGCATGGGCTCTATCTCGGGTCCGGGTTTAACTCCACCATTCCTAACCCTAGGGGAATATGAGGAGAGTCT
TAGAATACTCAACAATGTAATCGATGCATTAAGGAGTGAGGGATGTATCGATTACAGGGGTGTTTTAGCTGGCCAAATGA
TGCTAACGTGGCTGTGGGGTCCAACCGTTATAGAATTCTACAGTAGGTTTGGCGACCCCGAGACCGGTAACCTCCTATAC
ATGCTGGAAGATGATGTGCTTGAAGTATTCGAGAAAGCTGCCTCAGGTAAGTCACTGGGGAAGCTCGGAATTAGGGACAA
TGTTGTCGTTGTGAACAAGGCTATAGCCCCTCTCGGTTATCCAACTGATAGGAGACTAGCTACCGGGCTCAGGGTTAAAG
TAGATGAGGCCTCGATAAGGGATACTGGATGCACTATTCTATATGGAAGCATTGAGGTAGGGAGCGATGGATACCTGTAC
ACTAAGGGATCCAGGTTAGCCGAGATAGTTTGTTGGGGCTACAACTATGAAGAAGCATATAGGAAAACTGAGAATGCCAT
GAGATATATTGAAGGCGAAGCCCTCTTATTCCATAGAGGAGATATTGGTTCCCCTGAGTTGATGCAAGAGAAGATTAGTA
TAGCTGAGAAGATTAGACGTGTCTATACCTCAAGGGCTAGGCGTGGACTGCTGGGTGCATATATGGCATGGGTTCCAGGG
ATAGGCTTCATAGAGAACCCTCTGGTAAACCCCTACATGTCTAAACGTGCTCACGGGTGA

Upstream 100 bases:

>100_bases
CTATAACATGGATACCAATAGATGTATTCGAAGAAGTCATGAAATCCTTCGGCGTTAATCCGTGTTTCTCATGTTTTATT
GATAGCAGAGGGGATCATGC

Downstream 100 bases:

>100_bases
GACCTATGTCCAAATTCAACCTATTCAAACCCCTTATTGAAATCGGTCCCCCTCTCTCAAGTAGAGCAGTGAAAATACTC
TTGCTCGGCAGCGGGGAACT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKILLIGSSAREHALGLMMLKSGLEPRVYVVSEYRNPGLERVAGSSSGRIFITRVTEPGRILEIAEAVSPDLVVIGPEEP
LFAGISDTLREHGFAVFGASSKCAEIERSKVFARRLMWRHGINGRLFYASFSNVEDAVSFAEHAGDIVVKPARQVGGKGV
KVFSNTKSYLFNEISRVTGEYVRDLFSEMNAYRDIDEKILVELRVEGVEYTLHVISSGGSITPLPVIQDHPHAFEYDVGP
ETGGMGSISGPGLTPPFLTLGEYEESLRILNNVIDALRSEGCIDYRGVLAGQMMLTWLWGPTVIEFYSRFGDPETGNLLY
MLEDDVLEVFEKAASGKSLGKLGIRDNVVVVNKAIAPLGYPTDRRLATGLRVKVDEASIRDTGCTILYGSIEVGSDGYLY
TKGSRLAEIVCWGYNYEEAYRKTENAMRYIEGEALLFHRGDIGSPELMQEKISIAEKIRRVYTSRARRGLLGAYMAWVPG
IGFIENPLVNPYMSKRAHG

Sequences:

>Translated_499_residues
MKILLIGSSAREHALGLMMLKSGLEPRVYVVSEYRNPGLERVAGSSSGRIFITRVTEPGRILEIAEAVSPDLVVIGPEEP
LFAGISDTLREHGFAVFGASSKCAEIERSKVFARRLMWRHGINGRLFYASFSNVEDAVSFAEHAGDIVVKPARQVGGKGV
KVFSNTKSYLFNEISRVTGEYVRDLFSEMNAYRDIDEKILVELRVEGVEYTLHVISSGGSITPLPVIQDHPHAFEYDVGP
ETGGMGSISGPGLTPPFLTLGEYEESLRILNNVIDALRSEGCIDYRGVLAGQMMLTWLWGPTVIEFYSRFGDPETGNLLY
MLEDDVLEVFEKAASGKSLGKLGIRDNVVVVNKAIAPLGYPTDRRLATGLRVKVDEASIRDTGCTILYGSIEVGSDGYLY
TKGSRLAEIVCWGYNYEEAYRKTENAMRYIEGEALLFHRGDIGSPELMQEKISIAEKIRRVYTSRARRGLLGAYMAWVPG
IGFIENPLVNPYMSKRAHG
>Mature_499_residues
MKILLIGSSAREHALGLMMLKSGLEPRVYVVSEYRNPGLERVAGSSSGRIFITRVTEPGRILEIAEAVSPDLVVIGPEEP
LFAGISDTLREHGFAVFGASSKCAEIERSKVFARRLMWRHGINGRLFYASFSNVEDAVSFAEHAGDIVVKPARQVGGKGV
KVFSNTKSYLFNEISRVTGEYVRDLFSEMNAYRDIDEKILVELRVEGVEYTLHVISSGGSITPLPVIQDHPHAFEYDVGP
ETGGMGSISGPGLTPPFLTLGEYEESLRILNNVIDALRSEGCIDYRGVLAGQMMLTWLWGPTVIEFYSRFGDPETGNLLY
MLEDDVLEVFEKAASGKSLGKLGIRDNVVVVNKAIAPLGYPTDRRLATGLRVKVDEASIRDTGCTILYGSIEVGSDGYLY
TKGSRLAEIVCWGYNYEEAYRKTENAMRYIEGEALLFHRGDIGSPELMQEKISIAEKIRRVYTSRARRGLLGAYMAWVPG
IGFIENPLVNPYMSKRAHG

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=447, Percent_Identity=28.8590604026846, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4503915, Length=447, Percent_Identity=28.8590604026846, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI209869995, Length=447, Percent_Identity=28.8590604026846, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI209869993, Length=447, Percent_Identity=28.8590604026846, Blast_Score=154, Evalue=3e-37,
Organism=Escherichia coli, GI1790438, Length=454, Percent_Identity=29.295154185022, Blast_Score=168, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17567511, Length=460, Percent_Identity=27.3913043478261, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321203, Length=457, Percent_Identity=29.5404814004376, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI62471619, Length=368, Percent_Identity=34.7826086956522, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24582400, Length=368, Percent_Identity=34.7826086956522, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 55177; Mature: 55177

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLIGSSAREHALGLMMLKSGLEPRVYVVSEYRNPGLERVAGSSSGRIFITRVTEPGR
CEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCEEEEEEECCCCH
ILEIAEAVSPDLVVIGPEEPLFAGISDTLREHGFAVFGASSKCAEIERSKVFARRLMWRH
HHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHC
GINGRLFYASFSNVEDAVSFAEHAGDIVVKPARQVGGKGVKVFSNTKSYLFNEISRVTGE
CCCCEEEEEECCCHHHHHHHHHHCCCEEEECHHHCCCCCEEEECCCHHHHHHHHHHHHHH
YVRDLFSEMNAYRDIDEKILVELRVEGVEYTLHVISSGGSITPLPVIQDHPHAFEYDVGP
HHHHHHHHHHHHHCCCHHEEEEEEECCEEEEEEEEECCCCCCCCCCCCCCCCCEEECCCC
ETGGMGSISGPGLTPPFLTLGEYEESLRILNNVIDALRSEGCIDYRGVLAGQMMLTWLWG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
PTVIEFYSRFGDPETGNLLYMLEDDVLEVFEKAASGKSLGKLGIRDNVVVVNKAIAPLGY
HHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC
PTDRRLATGLRVKVDEASIRDTGCTILYGSIEVGSDGYLYTKGSRLAEIVCWGYNYEEAY
CCCCHHHCCCEEEECCCCCCCCCCEEEEEEEEECCCCEEEECCCCEEEEEEECCCHHHHH
RKTENAMRYIEGEALLFHRGDIGSPELMQEKISIAEKIRRVYTSRARRGLLGAYMAWVPG
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IGFIENPLVNPYMSKRAHG
CCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MKILLIGSSAREHALGLMMLKSGLEPRVYVVSEYRNPGLERVAGSSSGRIFITRVTEPGR
CEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCEEEEEEECCCCH
ILEIAEAVSPDLVVIGPEEPLFAGISDTLREHGFAVFGASSKCAEIERSKVFARRLMWRH
HHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHC
GINGRLFYASFSNVEDAVSFAEHAGDIVVKPARQVGGKGVKVFSNTKSYLFNEISRVTGE
CCCCEEEEEECCCHHHHHHHHHHCCCEEEECHHHCCCCCEEEECCCHHHHHHHHHHHHHH
YVRDLFSEMNAYRDIDEKILVELRVEGVEYTLHVISSGGSITPLPVIQDHPHAFEYDVGP
HHHHHHHHHHHHHCCCHHEEEEEEECCEEEEEEEEECCCCCCCCCCCCCCCCCEEECCCC
ETGGMGSISGPGLTPPFLTLGEYEESLRILNNVIDALRSEGCIDYRGVLAGQMMLTWLWG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
PTVIEFYSRFGDPETGNLLYMLEDDVLEVFEKAASGKSLGKLGIRDNVVVVNKAIAPLGY
HHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCC
PTDRRLATGLRVKVDEASIRDTGCTILYGSIEVGSDGYLYTKGSRLAEIVCWGYNYEEAY
CCCCHHHCCCEEEECCCCCCCCCCEEEEEEEEECCCCEEEECCCCEEEEEEECCCHHHHH
RKTENAMRYIEGEALLFHRGDIGSPELMQEKISIAEKIRRVYTSRARRGLLGAYMAWVPG
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IGFIENPLVNPYMSKRAHG
CCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]