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The map label for this gene is purT [H]

Identifier: 218883837

GI number: 218883837

Start: 493968

End: 495224

Strand: Direct

Name: purT [H]

Synonym: DKAM_0526

Alternate gene names: 218883837

Gene position: 493968-495224 (Clockwise)

Preceding gene: 218883836

Following gene: 218883838

Centisome position: 36.18

GC content: 47.57

Gene sequence:

>1257_bases
ATGTCCAAATTCAACCTATTCAAACCCCTTATTGAAATCGGTCCCCCTCTCTCAAGTAGAGCAGTGAAAATACTCTTGCT
CGGCAGCGGGGAACTAGGTAAAGAGATAGCGTTGGAGGCGCAGAGACTAGGGTTGGAAGTTATATCCGTGGATAGATATG
ATAATGCGCCGGCAATGCATGTCGCACATAGGAGATACGTTATCGACATGATGAATGAGCAAGCCGTTAAAGCCATTGTG
AAGAGGGAGCAACCTGACCTGATAATCCCCGAGATCGAAGCTATCTCAACCACTGCTCTCAGGGAGCTTGAAGACGAGGG
CTACAGGGTCACTCCTAATGCGAGGGCTGTCGAGATCTCGATGAATAGGATAATATTAAGAGAATACGTTGTTAAAGAAG
TTGGATTACCAACCACCAGGTACGCTATCGCGGAGAGCCCTGAGGAAGCCTTCAAAGCATGTAATGAAATAGGTTATCCG
TGCATAATTAAGCCTGAGATGAGTAGCAGTGGACATGGACACGTGAAGATAACTAATGGATCCTTCGACCAGGTGAGGAA
AGCCTTCGAGGAGTCGATCAGGCATGCACGTGGGAGGAGCCGTAGAGTATTAGTTGAGGAATATGTTGAATTAGATGCGG
AGTTCACAGTGTTAACCTATAGACACATAAATATGGATGGTAGAGTGGAAACAATTACATGCGATCCCATCGAGCATTGG
AGGTATGGCGAGTTCCATTATATAGAGTCATGGCAACCGTCGATCAGGTCAGCTGAGATCCTTGGCAAGATAGCTGAGAT
AGGGGTTAAGATAGCTGACTCCTTTGGAGGGCTTGGAATATATGGTGTCGAAGTATTCCTTACAAGGGATAATAGGGTGT
TATTCAGTGAGGTGGCCCCGAGGCCGCATGACACAGGCTTGGTAACCATTGCGAGCCAGGAGCTAAGCGAGTTCGCTATA
CACTTAAGGGCTGGACTAGGTCTTCCAGTGCCGAGGCCGAAGGTGGTCAATCCGGCTGCTAGTCTAGCCATATACTCGGA
GATAGATACATGGTCACCTGTAATTCACGGTATTAGGGAAGCCATCTCCATACCTGGTGTCGACATTAGGATATTCGGTA
AACCGTATGTATACACTGGGAGAAGAGTAGCAGTCCTACTAGCCAGGGGAGACACAGTAAGTGAAGCATTGGATAAGGTT
AGGAAAGCTGGAAGACGGCTTAAAGTAGTGGAGGGATGGGGTAAGGATGCCGTGTAA

Upstream 100 bases:

>100_bases
GACTGCTGGGTGCATATATGGCATGGGTTCCAGGGATAGGCTTCATAGAGAACCCTCTGGTAAACCCCTACATGTCTAAA
CGTGCTCACGGGTGAGACCT

Downstream 100 bases:

>100_bases
GGGTAAGGTGGCTGTAATAGTTGGAAGCGAGCGGGATCTACCTCATGTAGAGAAAGCATTGAAGATACTCAAAGAGAACA
ATATAGACGTTGATGTCAGG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MSKFNLFKPLIEIGPPLSSRAVKILLLGSGELGKEIALEAQRLGLEVISVDRYDNAPAMHVAHRRYVIDMMNEQAVKAIV
KREQPDLIIPEIEAISTTALRELEDEGYRVTPNARAVEISMNRIILREYVVKEVGLPTTRYAIAESPEEAFKACNEIGYP
CIIKPEMSSSGHGHVKITNGSFDQVRKAFEESIRHARGRSRRVLVEEYVELDAEFTVLTYRHINMDGRVETITCDPIEHW
RYGEFHYIESWQPSIRSAEILGKIAEIGVKIADSFGGLGIYGVEVFLTRDNRVLFSEVAPRPHDTGLVTIASQELSEFAI
HLRAGLGLPVPRPKVVNPAASLAIYSEIDTWSPVIHGIREAISIPGVDIRIFGKPYVYTGRRVAVLLARGDTVSEALDKV
RKAGRRLKVVEGWGKDAV

Sequences:

>Translated_418_residues
MSKFNLFKPLIEIGPPLSSRAVKILLLGSGELGKEIALEAQRLGLEVISVDRYDNAPAMHVAHRRYVIDMMNEQAVKAIV
KREQPDLIIPEIEAISTTALRELEDEGYRVTPNARAVEISMNRIILREYVVKEVGLPTTRYAIAESPEEAFKACNEIGYP
CIIKPEMSSSGHGHVKITNGSFDQVRKAFEESIRHARGRSRRVLVEEYVELDAEFTVLTYRHINMDGRVETITCDPIEHW
RYGEFHYIESWQPSIRSAEILGKIAEIGVKIADSFGGLGIYGVEVFLTRDNRVLFSEVAPRPHDTGLVTIASQELSEFAI
HLRAGLGLPVPRPKVVNPAASLAIYSEIDTWSPVIHGIREAISIPGVDIRIFGKPYVYTGRRVAVLLARGDTVSEALDKV
RKAGRRLKVVEGWGKDAV
>Mature_417_residues
SKFNLFKPLIEIGPPLSSRAVKILLLGSGELGKEIALEAQRLGLEVISVDRYDNAPAMHVAHRRYVIDMMNEQAVKAIVK
REQPDLIIPEIEAISTTALRELEDEGYRVTPNARAVEISMNRIILREYVVKEVGLPTTRYAIAESPEEAFKACNEIGYPC
IIKPEMSSSGHGHVKITNGSFDQVRKAFEESIRHARGRSRRVLVEEYVELDAEFTVLTYRHINMDGRVETITCDPIEHWR
YGEFHYIESWQPSIRSAEILGKIAEIGVKIADSFGGLGIYGVEVFLTRDNRVLFSEVAPRPHDTGLVTIASQELSEFAIH
LRAGLGLPVPRPKVVNPAASLAIYSEIDTWSPVIHGIREAISIPGVDIRIFGKPYVYTGRRVAVLLARGDTVSEALDKVR
KAGRRLKVVEGWGKDAV

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=397, Percent_Identity=44.5843828715365, Blast_Score=319, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6324702, Length=372, Percent_Identity=25.8064516129032, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 46672; Mature: 46541

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFNLFKPLIEIGPPLSSRAVKILLLGSGELGKEIALEAQRLGLEVISVDRYDNAPAMH
CCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHH
VAHRRYVIDMMNEQAVKAIVKREQPDLIIPEIEAISTTALRELEDEGYRVTPNARAVEIS
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCEEECCCCEEEEEE
MNRIILREYVVKEVGLPTTRYAIAESPEEAFKACNEIGYPCIIKPEMSSSGHGHVKITNG
HHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECC
SFDQVRKAFEESIRHARGRSRRVLVEEYVELDAEFTVLTYRHINMDGRVETITCDPIEHW
CHHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCEEEEEEEEECCCCEEEEEEECCHHHC
RYGEFHYIESWQPSIRSAEILGKIAEIGVKIADSFGGLGIYGVEVFLTRDNRVLFSEVAP
CCCCEEEECCCCCCHHHHHHHHHHHHHCHHEECCCCCCEEEEEEEEEEECCEEEEECCCC
RPHDTGLVTIASQELSEFAIHLRAGLGLPVPRPKVVNPAASLAIYSEIDTWSPVIHGIRE
CCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHH
AISIPGVDIRIFGKPYVYTGRRVAVLLARGDTVSEALDKVRKAGRRLKVVEGWGKDAV
HHCCCCEEEEEECCCEEEECCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCC
>Mature Secondary Structure 
SKFNLFKPLIEIGPPLSSRAVKILLLGSGELGKEIALEAQRLGLEVISVDRYDNAPAMH
CCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCHHH
VAHRRYVIDMMNEQAVKAIVKREQPDLIIPEIEAISTTALRELEDEGYRVTPNARAVEIS
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCEEECCCCEEEEEE
MNRIILREYVVKEVGLPTTRYAIAESPEEAFKACNEIGYPCIIKPEMSSSGHGHVKITNG
HHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECC
SFDQVRKAFEESIRHARGRSRRVLVEEYVELDAEFTVLTYRHINMDGRVETITCDPIEHW
CHHHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCCEEEEEEEEECCCCEEEEEEECCHHHC
RYGEFHYIESWQPSIRSAEILGKIAEIGVKIADSFGGLGIYGVEVFLTRDNRVLFSEVAP
CCCCEEEECCCCCCHHHHHHHHHHHHHCHHEECCCCCCEEEEEEEEEEECCEEEEECCCC
RPHDTGLVTIASQELSEFAIHLRAGLGLPVPRPKVVNPAASLAIYSEIDTWSPVIHGIRE
CCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEECCCCCHHHHHHHHHH
AISIPGVDIRIFGKPYVYTGRRVAVLLARGDTVSEALDKVRKAGRRLKVVEGWGKDAV
HHCCCCEEEEEECCCEEEECCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]