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The map label for this gene is guaA [H]

Identifier: 218883790

GI number: 218883790

Start: 445858

End: 447429

Strand: Reverse

Name: guaA [H]

Synonym: DKAM_0479

Alternate gene names: 218883790

Gene position: 447429-445858 (Counterclockwise)

Preceding gene: 218883791

Following gene: 218883789

Centisome position: 32.77

GC content: 47.58

Gene sequence:

>1572_bases
ATGACACGGGAAAACATTATCAATATACCTGGAAGCAGGATCCTGATAGTGGACTACGGTGGGCAGTACACACATTTAAT
AGCTAGGAGAATCAGGGAACTCGAAGTATACTCCGAGATAGTCTTCTACAGGGATTTAAACCGCGTTAACATCGAGGACT
ATGACGCGGTAATATTATCAGGCAGCCATTTAAGCGTGAAAAGCCTTGACAAAGACGCCGTGGAGACAGCTAGGAAGATC
CTCATGGAGGGAAATAAGCCATTACTAGGAATATGCTTCGGCCACCAGTTAATAGGATACCTCCTCGGTGCCAAGCTGTC
TACAGGGTGCAGCGAGTATGGTAGAACCACCGTGGAGATACTGAGTAGTGATGCATTATTTAATGGATGGGGCAACAGCG
AGATAGTCTGGATGAGCCACGGCGAATGCATCGAAGACCTCCCCGACTCCATTACATTACTAGCTGAAAGCGAGAATAAA
GTGGTGGCAGCTATTAAAGCAGTCGTGAACAACAGGGTTGTCTACGGTGTCCAATTTCATCCAGAGGTACATCACACTGT
GAAAGGCCGGAGGCTCCTGGATAACTTCCTCGGGATCATTAATGCGAAAAGGGCTTGGAGGACCGAGAACTACTACAGGT
ACATAATTGATGAACTCGGCAGGGAGGTAACCAGGAATGGTGTAGCCGTTGTCGGTGTCAGCGGCGGCATAGATTCAACT
GTTACAGCCTTGATAGGGAAGAAGCTCTTCAACGATAAATTAATACCCGTGTTAGTTGATCATGGTTTATTGAGGGAGGG
GGAAAGAGAGCTTGTTGTCACATCCCTCGAGAAACTTGGGTTAAACCCCTTAATAGTGGATGCCGGGGAGAGATTCATCT
CGAAGCTCGAGGGCTCCACTGACTGCGAGGAGAGGCGTAGAGTAATAGGTGAAGAGTTTGCGAGAATATTCTCTGAAATC
ATTGAAAAAGAAGGTGCTGAATACTTCCTACAGGGTACAACATACCCTGATGTAATCGAGAGTGGTGGAACCCGTAAGGC
AGCCTCAGTGATCAAATCACACCACAACGTCGGAGGATTACCCGAGTGGTTTAGGGGTAGGGTTAAGATATTGGAGCCAC
TGCGTTTCCTCTACAAGGATGAGGTGAGAGAGATAGCTAGGTTACTCGGTGTTCCCCGTGAAATAATCGAGAGACATCCG
TTTCCAGGACCTGGGCTAGCTGTCAGGATTACAGGTGTCTTCACAAAGAAGAAGCTGGATGTGTGTAGGAGGGCCTCTAA
GATAGTTGAGGATGTGCTCAGAGAGTATGGGTTATACAGTAGAGTATGGCAGGCCTTTGCCGTTATAGGCGATGATAGAT
GGGTCGGGGTAAAGGGTGATGCTAGAAGCCACGGTTACATAGTCACCGTTAGAATAGTCGAAAGCGTTGACGGGATGACT
GCCGACTACTCAAAGATACCCTATGATGTACTCGATGAGATTAGTAGGAGGATCACTAATAGTATCCAGGATGTGACAAT
GGTTACCTATGCTATCACATCCAAGCCTCCCAGCACTATAGAGCCCTGCTGA

Upstream 100 bases:

>100_bases
TAATAGATTTAATGTAAAACCGGGCCTGTGAAACAATGATTTTATACATGAAAACCATTTAAACCCTGTTTTTAAGACAT
AGCCGGCTATACTATTCATC

Downstream 100 bases:

>100_bases
GGCGGTGTGGACTTGAGGATCATCTACATACCATGGAGTAAAGCCATAGAGTTATGCTACAGGCTTGCATCCATGATACT
TGATAGCGGGAAGCGATACG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MTRENIINIPGSRILIVDYGGQYTHLIARRIRELEVYSEIVFYRDLNRVNIEDYDAVILSGSHLSVKSLDKDAVETARKI
LMEGNKPLLGICFGHQLIGYLLGAKLSTGCSEYGRTTVEILSSDALFNGWGNSEIVWMSHGECIEDLPDSITLLAESENK
VVAAIKAVVNNRVVYGVQFHPEVHHTVKGRRLLDNFLGIINAKRAWRTENYYRYIIDELGREVTRNGVAVVGVSGGIDST
VTALIGKKLFNDKLIPVLVDHGLLREGERELVVTSLEKLGLNPLIVDAGERFISKLEGSTDCEERRRVIGEEFARIFSEI
IEKEGAEYFLQGTTYPDVIESGGTRKAASVIKSHHNVGGLPEWFRGRVKILEPLRFLYKDEVREIARLLGVPREIIERHP
FPGPGLAVRITGVFTKKKLDVCRRASKIVEDVLREYGLYSRVWQAFAVIGDDRWVGVKGDARSHGYIVTVRIVESVDGMT
ADYSKIPYDVLDEISRRITNSIQDVTMVTYAITSKPPSTIEPC

Sequences:

>Translated_523_residues
MTRENIINIPGSRILIVDYGGQYTHLIARRIRELEVYSEIVFYRDLNRVNIEDYDAVILSGSHLSVKSLDKDAVETARKI
LMEGNKPLLGICFGHQLIGYLLGAKLSTGCSEYGRTTVEILSSDALFNGWGNSEIVWMSHGECIEDLPDSITLLAESENK
VVAAIKAVVNNRVVYGVQFHPEVHHTVKGRRLLDNFLGIINAKRAWRTENYYRYIIDELGREVTRNGVAVVGVSGGIDST
VTALIGKKLFNDKLIPVLVDHGLLREGERELVVTSLEKLGLNPLIVDAGERFISKLEGSTDCEERRRVIGEEFARIFSEI
IEKEGAEYFLQGTTYPDVIESGGTRKAASVIKSHHNVGGLPEWFRGRVKILEPLRFLYKDEVREIARLLGVPREIIERHP
FPGPGLAVRITGVFTKKKLDVCRRASKIVEDVLREYGLYSRVWQAFAVIGDDRWVGVKGDARSHGYIVTVRIVESVDGMT
ADYSKIPYDVLDEISRRITNSIQDVTMVTYAITSKPPSTIEPC
>Mature_522_residues
TRENIINIPGSRILIVDYGGQYTHLIARRIRELEVYSEIVFYRDLNRVNIEDYDAVILSGSHLSVKSLDKDAVETARKIL
MEGNKPLLGICFGHQLIGYLLGAKLSTGCSEYGRTTVEILSSDALFNGWGNSEIVWMSHGECIEDLPDSITLLAESENKV
VAAIKAVVNNRVVYGVQFHPEVHHTVKGRRLLDNFLGIINAKRAWRTENYYRYIIDELGREVTRNGVAVVGVSGGIDSTV
TALIGKKLFNDKLIPVLVDHGLLREGERELVVTSLEKLGLNPLIVDAGERFISKLEGSTDCEERRRVIGEEFARIFSEII
EKEGAEYFLQGTTYPDVIESGGTRKAASVIKSHHNVGGLPEWFRGRVKILEPLRFLYKDEVREIARLLGVPREIIERHPF
PGPGLAVRITGVFTKKKLDVCRRASKIVEDVLREYGLYSRVWQAFAVIGDDRWVGVKGDARSHGYIVTVRIVESVDGMTA
DYSKIPYDVLDEISRRITNSIQDVTMVTYAITSKPPSTIEPC

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4504035, Length=568, Percent_Identity=31.8661971830986, Blast_Score=230, Evalue=3e-60,
Organism=Escherichia coli, GI1788854, Length=532, Percent_Identity=39.6616541353383, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71992717, Length=576, Percent_Identity=32.4652777777778, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI71992710, Length=576, Percent_Identity=32.4652777777778, Blast_Score=226, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI133901714, Length=576, Percent_Identity=32.4652777777778, Blast_Score=226, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6323873, Length=519, Percent_Identity=40.2697495183044, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281365319, Length=513, Percent_Identity=32.1637426900585, Blast_Score=222, Evalue=6e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 58661; Mature: 58530

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRENIINIPGSRILIVDYGGQYTHLIARRIRELEVYSEIVFYRDLNRVNIEDYDAVILS
CCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
GSHLSVKSLDKDAVETARKILMEGNKPLLGICFGHQLIGYLLGAKLSTGCSEYGRTTVEI
CCCCCHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCHHHCCHHHHCCCEEEH
LSSDALFNGWGNSEIVWMSHGECIEDLPDSITLLAESENKVVAAIKAVVNNRVVYGVQFH
HHCCCEECCCCCCEEEEEECCCHHHHCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEC
PEVHHTVKGRRLLDNFLGIINAKRAWRTENYYRYIIDELGREVTRNGVAVVGVSGGIDST
CCHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHH
VTALIGKKLFNDKLIPVLVDHGLLREGERELVVTSLEKLGLNPLIVDAGERFISKLEGST
HHHHHHHHHHCCCCCHHHHCCHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCC
DCEERRRVIGEEFARIFSEIIEKEGAEYFLQGTTYPDVIESGGTRKAASVIKSHHNVGGL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCCC
PEWFRGRVKILEPLRFLYKDEVREIARLLGVPREIIERHPFPGPGLAVRITGVFTKKKLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEEEEHHHHHHH
VCRRASKIVEDVLREYGLYSRVWQAFAVIGDDRWVGVKGDARSHGYIVTVRIVESVDGMT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCCC
ADYSKIPYDVLDEISRRITNSIQDVTMVTYAITSKPPSTIEPC
CCHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TRENIINIPGSRILIVDYGGQYTHLIARRIRELEVYSEIVFYRDLNRVNIEDYDAVILS
CCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
GSHLSVKSLDKDAVETARKILMEGNKPLLGICFGHQLIGYLLGAKLSTGCSEYGRTTVEI
CCCCCHHHCCHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCHHHCCHHHHCCCEEEH
LSSDALFNGWGNSEIVWMSHGECIEDLPDSITLLAESENKVVAAIKAVVNNRVVYGVQFH
HHCCCEECCCCCCEEEEEECCCHHHHCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEC
PEVHHTVKGRRLLDNFLGIINAKRAWRTENYYRYIIDELGREVTRNGVAVVGVSGGIDST
CCHHHHHHHHHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCHHH
VTALIGKKLFNDKLIPVLVDHGLLREGERELVVTSLEKLGLNPLIVDAGERFISKLEGST
HHHHHHHHHHCCCCCHHHHCCHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCC
DCEERRRVIGEEFARIFSEIIEKEGAEYFLQGTTYPDVIESGGTRKAASVIKSHHNVGGL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCHHHHHHHHHCCCCCCC
PEWFRGRVKILEPLRFLYKDEVREIARLLGVPREIIERHPFPGPGLAVRITGVFTKKKLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEEEEHHHHHHH
VCRRASKIVEDVLREYGLYSRVWQAFAVIGDDRWVGVKGDARSHGYIVTVRIVESVDGMT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCCCCC
ADYSKIPYDVLDEISRRITNSIQDVTMVTYAITSKPPSTIEPC
CCHHCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792869 [H]