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The map label for this gene is asnS [H]

Identifier: 218883748

GI number: 218883748

Start: 406168

End: 407334

Strand: Direct

Name: asnS [H]

Synonym: DKAM_0437

Alternate gene names: 218883748

Gene position: 406168-407334 (Clockwise)

Preceding gene: 218883747

Following gene: 218883749

Centisome position: 29.75

GC content: 44.22

Gene sequence:

>1167_bases
GTGCATAAACTATTCTTAGATGAAGAAATAGCCATATATGCAAGAATAAAACCCCTCGACTCCTCGAGAATCCTTGTAAG
AGATGCCACAGGCGAGAAAGAGGTACGAATCCTGGGTGAGAAGGCTAGGGATATTACTTCACTACCATGTGAGTCAACAG
TATTAATAACCGGGGTGCTAACTGAGCAGGGATTGAAGGTGAAAGACTTCAAGGTTCTCCACAAACCATTAGAGCCCGCG
AAAATATGCGTGGATAACGTACCCTTGGATCCGGCCGAGTATGTGAGAAACTACCCCATATATATTAGACGCCCTGAAAT
ACTACGGGTTGTTAAAACATACTCTATAGTATTGAGGACCATGAGACGTATACTTGATGAGCACGGCTTCACGGAATTGC
CAGCCCCAATAATAGGTTATGTAAGCGATCCAGGTCTGCGGGGAGCGGAAAAGGCCATTGTCAAAATATATGGTGAGACA
TATGAGGTACAAAGTAGTGTCATCATGTATAAACAATTATATGCATCACTACTCGAGAAGATATATTACGTAGCCAGGAA
CCTTAGAATAGAGCCACCTGAAAACATGTATACTGGGAGACACCTAGTCGAGTTCACTCAAGTGGATGTCGAAGCCTCTA
TAACCACAGCCAGCGATATGATGAGGCTGGCTGAGGAAACACTATATAAAACAGTGAGATATATGCTCAACCATTACAGC
GAGCTCCTAACATACGATCAGGTAGATTTCCTAGAGAAGACTATTACAAAACCACCCTACCCTAGGATAGACTATGACTC
AGCACTAGGGTTGTTGGAGAGAAAAGGGTATAGACTTGAACACGGTAAGGAAATAAGCTTCGAGGCCGAGGCAGTACTAG
GAGACATGTATGGGACCCCAGTGTGGTTACACGGCTTCCCCACGGTAAGCAGGGGCTTCTATTATATGCCGGATCCCGAG
AAACCGGGATATAATATAGACTACAACCTGATTCTACCAGGCCATGCAGGTGAAGTACTTGATGGTGGATGCAGGGAGTA
TAGGTATGAGAAACTAGTTGAAAGAATAAAGTATCATGGAGAACCGCTTGAGAAATACAGGTGGTTCCTCGAGCTGGCTA
AAGCGGGGGCAGAAACGTTTATTAAGGTCATTGAGGAAATTTTATAG

Upstream 100 bases:

>100_bases
ATTCGGGTTTATATCTATAAAGATTTAAAAACCATCCAGAGGATATTATGTGCTCAATAGGTGAAAGGGATGTTATTTAA
CACGTAGTCTAAAAATCCCT

Downstream 100 bases:

>100_bases
GCAGTTGAGGTCGAATGTTGGAGAGGTTGTTGAAGCCTAGGGAGTTCTGCGAGATTGTTGGGATTAGCTACGGGACTTTT
AAGCGGTGGGTCTCTCAAGG

Product: tRNA synthetase, class II (D, K and N)

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MHKLFLDEEIAIYARIKPLDSSRILVRDATGEKEVRILGEKARDITSLPCESTVLITGVLTEQGLKVKDFKVLHKPLEPA
KICVDNVPLDPAEYVRNYPIYIRRPEILRVVKTYSIVLRTMRRILDEHGFTELPAPIIGYVSDPGLRGAEKAIVKIYGET
YEVQSSVIMYKQLYASLLEKIYYVARNLRIEPPENMYTGRHLVEFTQVDVEASITTASDMMRLAEETLYKTVRYMLNHYS
ELLTYDQVDFLEKTITKPPYPRIDYDSALGLLERKGYRLEHGKEISFEAEAVLGDMYGTPVWLHGFPTVSRGFYYMPDPE
KPGYNIDYNLILPGHAGEVLDGGCREYRYEKLVERIKYHGEPLEKYRWFLELAKAGAETFIKVIEEIL

Sequences:

>Translated_388_residues
MHKLFLDEEIAIYARIKPLDSSRILVRDATGEKEVRILGEKARDITSLPCESTVLITGVLTEQGLKVKDFKVLHKPLEPA
KICVDNVPLDPAEYVRNYPIYIRRPEILRVVKTYSIVLRTMRRILDEHGFTELPAPIIGYVSDPGLRGAEKAIVKIYGET
YEVQSSVIMYKQLYASLLEKIYYVARNLRIEPPENMYTGRHLVEFTQVDVEASITTASDMMRLAEETLYKTVRYMLNHYS
ELLTYDQVDFLEKTITKPPYPRIDYDSALGLLERKGYRLEHGKEISFEAEAVLGDMYGTPVWLHGFPTVSRGFYYMPDPE
KPGYNIDYNLILPGHAGEVLDGGCREYRYEKLVERIKYHGEPLEKYRWFLELAKAGAETFIKVIEEIL
>Mature_388_residues
MHKLFLDEEIAIYARIKPLDSSRILVRDATGEKEVRILGEKARDITSLPCESTVLITGVLTEQGLKVKDFKVLHKPLEPA
KICVDNVPLDPAEYVRNYPIYIRRPEILRVVKTYSIVLRTMRRILDEHGFTELPAPIIGYVSDPGLRGAEKAIVKIYGET
YEVQSSVIMYKQLYASLLEKIYYVARNLRIEPPENMYTGRHLVEFTQVDVEASITTASDMMRLAEETLYKTVRYMLNHYS
ELLTYDQVDFLEKTITKPPYPRIDYDSALGLLERKGYRLEHGKEISFEAEAVLGDMYGTPVWLHGFPTVSRGFYYMPDPE
KPGYNIDYNLILPGHAGEVLDGGCREYRYEKLVERIKYHGEPLEKYRWFLELAKAGAETFIKVIEEIL

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=295, Percent_Identity=26.4406779661017, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI226491502, Length=404, Percent_Identity=23.5148514851485, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4758762, Length=376, Percent_Identity=23.6702127659574, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1787161, Length=371, Percent_Identity=24.2587601078167, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=378, Percent_Identity=23.8095238095238, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=290, Percent_Identity=27.5862068965517, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=286, Percent_Identity=24.4755244755245, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6321807, Length=296, Percent_Identity=26.0135135135135, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323011, Length=283, Percent_Identity=28.2685512367491, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136276, Length=306, Percent_Identity=26.797385620915, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921528, Length=367, Percent_Identity=23.7057220708447, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 44823; Mature: 44823

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKLFLDEEIAIYARIKPLDSSRILVRDATGEKEVRILGEKARDITSLPCESTVLITGVL
CCCCEECCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEH
TEQGLKVKDFKVLHKPLEPAKICVDNVPLDPAEYVRNYPIYIRRPEILRVVKTYSIVLRT
HCCCCCCHHHHHHHCCCCHHHHHHCCCCCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
MRRILDEHGFTELPAPIIGYVSDPGLRGAEKAIVKIYGETYEVQSSVIMYKQLYASLLEK
HHHHHHHCCCCCCCCHHEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IYYVARNLRIEPPENMYTGRHLVEFTQVDVEASITTASDMMRLAEETLYKTVRYMLNHYS
HHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLTYDQVDFLEKTITKPPYPRIDYDSALGLLERKGYRLEHGKEISFEAEAVLGDMYGTP
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCC
VWLHGFPTVSRGFYYMPDPEKPGYNIDYNLILPGHAGEVLDGGCREYRYEKLVERIKYHG
EEEECCCHHHCCEEECCCCCCCCCCCEEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCC
EPLEKYRWFLELAKAGAETFIKVIEEIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MHKLFLDEEIAIYARIKPLDSSRILVRDATGEKEVRILGEKARDITSLPCESTVLITGVL
CCCCEECCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEH
TEQGLKVKDFKVLHKPLEPAKICVDNVPLDPAEYVRNYPIYIRRPEILRVVKTYSIVLRT
HCCCCCCHHHHHHHCCCCHHHHHHCCCCCCHHHHHCCCCEEEECHHHHHHHHHHHHHHHH
MRRILDEHGFTELPAPIIGYVSDPGLRGAEKAIVKIYGETYEVQSSVIMYKQLYASLLEK
HHHHHHHCCCCCCCCHHEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IYYVARNLRIEPPENMYTGRHLVEFTQVDVEASITTASDMMRLAEETLYKTVRYMLNHYS
HHHHHHCCCCCCCCCCCCCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLTYDQVDFLEKTITKPPYPRIDYDSALGLLERKGYRLEHGKEISFEAEAVLGDMYGTP
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCC
VWLHGFPTVSRGFYYMPDPEKPGYNIDYNLILPGHAGEVLDGGCREYRYEKLVERIKYHG
EEEECCCHHHCCEEECCCCCCCCCCCEEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCC
EPLEKYRWFLELAKAGAETFIKVIEEIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA