Click here to switch to the map view.

The map label for this gene is cysK [H]

Identifier: 218883689

GI number: 218883689

Start: 350818

End: 351825

Strand: Direct

Name: cysK [H]

Synonym: DKAM_0378

Alternate gene names: 218883689

Gene position: 350818-351825 (Clockwise)

Preceding gene: 218883685

Following gene: 218883690

Centisome position: 25.7

GC content: 46.63

Gene sequence:

>1008_bases
ATGAAGGTGTGTAGGGATATAACCGAGTGTATAGGGGGCACACCAATAATACGGCTCTCAAGGATACTGCCACAGGGCTT
CAAGGCCGAAGTATGGGGTAAGATGGAGTTCACTAATCCAACGGGCAGCGTGAAAGATAGGATGGCATTATACATGCTGA
GAAAAGCCGTTGAGAAAGGAGAATTGAAACCAGGTATGACAATAGTTGTTCCTACAACAGGTAATACAGGCATAGCTTTC
TCAGCCCTTGCAAGCGTGCTCGGCTTCAAGGTTTTAATAGTAATACCAGAGGAGATGAGTGCCGAGAGATTCATGTTAAT
GAAGTTATTCGGAGCCGAATTCTACTTCACACCAGGCGGTGAAGCCGATGCTGGTAATGCATTAGAAATAGCTAGAAAGC
TTGTGGCTGAAAACCCTAATAAATATTACTTATTCGATCAATGGGGAGATGAAGCCAATATACAGGCCCATTACGAGACA
ACAGGTAAAGAGATACTTGATCAAATAGGGTGCCCCAAGGCCTTTGTAGCTGAGGTTGGAACCGGAGGTACTTTAATAGG
TATAGCGAAGAGGCTTAAAGAGGAGTGCCGCGACGTCATAGTGGCGGGTGCTGAACCAGCTGAATGCCCGGTTGCAGCAG
AGTGGTTTAAGACTGGTAAGCCGGGTCCCTGGGGAAGACATGAAATAGAGGGAGTTGGTGACGGCTTCATACCCGATATA
GTTATGAGGAATAAGCACCTGCTCGACGACTTTGTAACCGTGAACAGTGATGAAGCAATACAAATGGCTAGGAAGATAGC
CAGGCTAGAAGGCCTCCCAGTTGGAATAAGCAGTGGAGCAAATGTTGTAGCAGCTATAAAGCTCGCACAAGCCCATAACC
TGAGAAACGACGACAAGGTTGTAACAATACTACCAGACTACGCTGCCAGATATTTCAGCACCAGGCTCTTCAAGAAGAAG
CGTGAAATAGTTAACAGGAAGCGGCTGCTGGAGGAGCTGGATATTTAG

Upstream 100 bases:

>100_bases
AGCCCGTGGGTAATAAATTTTTACAATTATAAACCGTAACAATGATTTAAATACAGGTTCCCACTTTAGTAAGATATATA
CTGAGAAGGAGGGTATATGC

Downstream 100 bases:

>100_bases
TCCCATACTATCCTATTCTTCTTTTCTTCTCTTCTTTCTTCAATTCCCTATCTTATCATATATGGTCTGGCTAGATAGAG
ACGACCTCATCTAGATGAAT

Product: Pyridoxal-5'-phosphate-dependent enzyme subunit beta

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKVCRDITECIGGTPIIRLSRILPQGFKAEVWGKMEFTNPTGSVKDRMALYMLRKAVEKGELKPGMTIVVPTTGNTGIAF
SALASVLGFKVLIVIPEEMSAERFMLMKLFGAEFYFTPGGEADAGNALEIARKLVAENPNKYYLFDQWGDEANIQAHYET
TGKEILDQIGCPKAFVAEVGTGGTLIGIAKRLKEECRDVIVAGAEPAECPVAAEWFKTGKPGPWGRHEIEGVGDGFIPDI
VMRNKHLLDDFVTVNSDEAIQMARKIARLEGLPVGISSGANVVAAIKLAQAHNLRNDDKVVTILPDYAARYFSTRLFKKK
REIVNRKRLLEELDI

Sequences:

>Translated_335_residues
MKVCRDITECIGGTPIIRLSRILPQGFKAEVWGKMEFTNPTGSVKDRMALYMLRKAVEKGELKPGMTIVVPTTGNTGIAF
SALASVLGFKVLIVIPEEMSAERFMLMKLFGAEFYFTPGGEADAGNALEIARKLVAENPNKYYLFDQWGDEANIQAHYET
TGKEILDQIGCPKAFVAEVGTGGTLIGIAKRLKEECRDVIVAGAEPAECPVAAEWFKTGKPGPWGRHEIEGVGDGFIPDI
VMRNKHLLDDFVTVNSDEAIQMARKIARLEGLPVGISSGANVVAAIKLAQAHNLRNDDKVVTILPDYAARYFSTRLFKKK
REIVNRKRLLEELDI
>Mature_335_residues
MKVCRDITECIGGTPIIRLSRILPQGFKAEVWGKMEFTNPTGSVKDRMALYMLRKAVEKGELKPGMTIVVPTTGNTGIAF
SALASVLGFKVLIVIPEEMSAERFMLMKLFGAEFYFTPGGEADAGNALEIARKLVAENPNKYYLFDQWGDEANIQAHYET
TGKEILDQIGCPKAFVAEVGTGGTLIGIAKRLKEECRDVIVAGAEPAECPVAAEWFKTGKPGPWGRHEIEGVGDGFIPDI
VMRNKHLLDDFVTVNSDEAIQMARKIARLEGLPVGISSGANVVAAIKLAQAHNLRNDDKVVTILPDYAARYFSTRLFKKK
REIVNRKRLLEELDI

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=327, Percent_Identity=39.4495412844037, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI295821200, Length=327, Percent_Identity=39.4495412844037, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI4557415, Length=327, Percent_Identity=39.4495412844037, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI11345492, Length=260, Percent_Identity=28.0769230769231, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788754, Length=321, Percent_Identity=40.809968847352, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI2367138, Length=321, Percent_Identity=33.9563862928349, Blast_Score=173, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17535051, Length=327, Percent_Identity=39.4495412844037, Blast_Score=190, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17562970, Length=321, Percent_Identity=37.0716510903427, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI32566674, Length=315, Percent_Identity=36.8253968253968, Blast_Score=180, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI115535073, Length=314, Percent_Identity=37.8980891719745, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17561720, Length=318, Percent_Identity=36.1635220125786, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17534315, Length=323, Percent_Identity=34.984520123839, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25147552, Length=326, Percent_Identity=32.5153374233129, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32566672, Length=228, Percent_Identity=36.4035087719298, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17561716, Length=130, Percent_Identity=36.9230769230769, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=321, Percent_Identity=33.6448598130841, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321449, Length=350, Percent_Identity=32, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24643623, Length=337, Percent_Identity=35.3115727002967, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI20129101, Length=337, Percent_Identity=35.3115727002967, Blast_Score=160, Evalue=9e-40,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 36902; Mature: 36902

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVCRDITECIGGTPIIRLSRILPQGFKAEVWGKMEFTNPTGSVKDRMALYMLRKAVEKG
CCHHHHHHHHHCCCHHHHHHHHCCCCCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHHC
ELKPGMTIVVPTTGNTGIAFSALASVLGFKVLIVIPEEMSAERFMLMKLFGAEFYFTPGG
CCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCEEEECCCC
EADAGNALEIARKLVAENPNKYYLFDQWGDEANIQAHYETTGKEILDQIGCPKAFVAEVG
CCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHCC
TGGTLIGIAKRLKEECRDVIVAGAEPAECPVAAEWFKTGKPGPWGRHEIEGVGDGFIPDI
CCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
VMRNKHLLDDFVTVNSDEAIQMARKIARLEGLPVGISSGANVVAAIKLAQAHNLRNDDKV
HHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCCEEHHHHHHHHHCCCCCCCE
VTILPDYAARYFSTRLFKKKREIVNRKRLLEELDI
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVCRDITECIGGTPIIRLSRILPQGFKAEVWGKMEFTNPTGSVKDRMALYMLRKAVEKG
CCHHHHHHHHHCCCHHHHHHHHCCCCCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHHC
ELKPGMTIVVPTTGNTGIAFSALASVLGFKVLIVIPEEMSAERFMLMKLFGAEFYFTPGG
CCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCEEEECCCC
EADAGNALEIARKLVAENPNKYYLFDQWGDEANIQAHYETTGKEILDQIGCPKAFVAEVG
CCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCHHHHHHCC
TGGTLIGIAKRLKEECRDVIVAGAEPAECPVAAEWFKTGKPGPWGRHEIEGVGDGFIPDI
CCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
VMRNKHLLDDFVTVNSDEAIQMARKIARLEGLPVGISSGANVVAAIKLAQAHNLRNDDKV
HHCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECCCCCCEEHHHHHHHHHCCCCCCCE
VTILPDYAARYFSTRLFKKKREIVNRKRLLEELDI
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA