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The map label for this gene is asnC [H]

Identifier: 218883641

GI number: 218883641

Start: 304057

End: 305355

Strand: Reverse

Name: asnC [H]

Synonym: DKAM_0330

Alternate gene names: 218883641

Gene position: 305355-304057 (Counterclockwise)

Preceding gene: 218883642

Following gene: 218883640

Centisome position: 22.37

GC content: 47.42

Gene sequence:

>1299_bases
ATGGAGTATAAGAGTATCGCTGAGATAATGAGCAATGATAAAATTGGTTCACACGTCTGTATAAGGGGCTGGATATATAG
GAGGAGTGTTGTAGGTGGTAAAGCATTTATCAGGGTAAGGGATTCATCCGGGGTAATACAGGTTGTAGTTGATTCAGCCA
GGCTTGGAGAAGCCCTTGTAGACGAGTTAAAGGATATAGGTTTGGAGGCGAGCGTTGTTGCTTGTGGAACTGTTAGAAGG
GAGGAAAGAGCACCCGGGGGATACGAGTTAGATGTCGACTTCTTTAAAATCATAGGCTATAGCCGGGACTTCCCGATAAA
GGGTGGCGAAGGCATAGAATATCTCCTGGATAACAGGCATTTATGGGTAAGGAGCCCAAGGTTCACGAAGATATTTAGGA
TAAAGCACACGATTCTTCAAGCCGGCAGAGAATACTTCACGAGGAATGGCTGGTGGGAAGTCAACCCGCCGATATTGACT
GCATCAGCCTGTGAGGGTGGTGCAACGTTATTCCCCGTTGAATACTTTGAGTCTAAGGCCTACTTGAGTCAGAGCGCGCA
ATTATATCTAGAGGCACTTATCTATGTTCTCGAGAAAGTATGGAGTCTAACCCCGAGTTTTAGAGCCGAGAAGTCTAGGA
CTAGGAGGCATCTCGCCGAGTACTGGCACCTAGAGCCTGAGGCAGCATGGTACAGCATGGATGACATGATGAAGGTCGCG
GAGGAGCTGGCATCGCATATTGTGACCAGGGTACTTGAGGAAAGGAGGAGTGAGCTAGTAGACCTGGGTAGGGATATCGA
GAAATTAAAGCCTGCAATGGAGACGCCCTACCCACGGATAAGGTATGATGAGGCGATAGAGATACTCCAGAAGAAAGGAG
TTAATGTTAAATGGGGCGATGATCTAGGCGCGGATGAGGAGAGGATACTCACCATGGAGTTCGAGAAACCGTTCTTCGTG
ACACACTTCCCAAGGCAGATAAAAAGCTTCTATATGAAGGTTGATCCATCAAACCCCAATGTAGTACTCGGCTTCGACCT
CTTAGCGCCTGAAGGATATGGTGAAATAGTTGGTGGAGGCGAGCGTGAGGACAGGTATGATGTATTATACCAGAAGATCA
TAGAGCAAGGATTGAACCCAGAGGACTATAAATGGTACCTGGATTTAAGGAAGTATGGAAGTGTGCCTCACAGCGGATTT
GGATTAGGGGTAGAAAGAGTTACGATGTGGATTACTGGGCTGGATCACATAAGGGATACACTACCGTTCCCCAGGTTCAG
GGGAAGAATCTACCCGTAG

Upstream 100 bases:

>100_bases
GGATTTGCGGTCTTCTAGAACTTAAAGCTGGTTAATATAGGAGTAGTGTTTTAACCTGGTTCACCATTTTAAACTAAAAC
TAGGGAAATAGGGGTTGGAA

Downstream 100 bases:

>100_bases
GCTAAAGGGTGTATATATCGTTGAATACTAGGATCGGGGATGAGGTGCTGAATAGGTTAAGGAAGGAGTATAAGCTGGAT
GCGGATGAATTCGTGGTATC

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MEYKSIAEIMSNDKIGSHVCIRGWIYRRSVVGGKAFIRVRDSSGVIQVVVDSARLGEALVDELKDIGLEASVVACGTVRR
EERAPGGYELDVDFFKIIGYSRDFPIKGGEGIEYLLDNRHLWVRSPRFTKIFRIKHTILQAGREYFTRNGWWEVNPPILT
ASACEGGATLFPVEYFESKAYLSQSAQLYLEALIYVLEKVWSLTPSFRAEKSRTRRHLAEYWHLEPEAAWYSMDDMMKVA
EELASHIVTRVLEERRSELVDLGRDIEKLKPAMETPYPRIRYDEAIEILQKKGVNVKWGDDLGADEERILTMEFEKPFFV
THFPRQIKSFYMKVDPSNPNVVLGFDLLAPEGYGEIVGGGEREDRYDVLYQKIIEQGLNPEDYKWYLDLRKYGSVPHSGF
GLGVERVTMWITGLDHIRDTLPFPRFRGRIYP

Sequences:

>Translated_432_residues
MEYKSIAEIMSNDKIGSHVCIRGWIYRRSVVGGKAFIRVRDSSGVIQVVVDSARLGEALVDELKDIGLEASVVACGTVRR
EERAPGGYELDVDFFKIIGYSRDFPIKGGEGIEYLLDNRHLWVRSPRFTKIFRIKHTILQAGREYFTRNGWWEVNPPILT
ASACEGGATLFPVEYFESKAYLSQSAQLYLEALIYVLEKVWSLTPSFRAEKSRTRRHLAEYWHLEPEAAWYSMDDMMKVA
EELASHIVTRVLEERRSELVDLGRDIEKLKPAMETPYPRIRYDEAIEILQKKGVNVKWGDDLGADEERILTMEFEKPFFV
THFPRQIKSFYMKVDPSNPNVVLGFDLLAPEGYGEIVGGGEREDRYDVLYQKIIEQGLNPEDYKWYLDLRKYGSVPHSGF
GLGVERVTMWITGLDHIRDTLPFPRFRGRIYP
>Mature_432_residues
MEYKSIAEIMSNDKIGSHVCIRGWIYRRSVVGGKAFIRVRDSSGVIQVVVDSARLGEALVDELKDIGLEASVVACGTVRR
EERAPGGYELDVDFFKIIGYSRDFPIKGGEGIEYLLDNRHLWVRSPRFTKIFRIKHTILQAGREYFTRNGWWEVNPPILT
ASACEGGATLFPVEYFESKAYLSQSAQLYLEALIYVLEKVWSLTPSFRAEKSRTRRHLAEYWHLEPEAAWYSMDDMMKVA
EELASHIVTRVLEERRSELVDLGRDIEKLKPAMETPYPRIRYDEAIEILQKKGVNVKWGDDLGADEERILTMEFEKPFFV
THFPRQIKSFYMKVDPSNPNVVLGFDLLAPEGYGEIVGGGEREDRYDVLYQKIIEQGLNPEDYKWYLDLRKYGSVPHSGF
GLGVERVTMWITGLDHIRDTLPFPRFRGRIYP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4758762, Length=440, Percent_Identity=35, Blast_Score=269, Evalue=4e-72,
Organism=Homo sapiens, GI226491502, Length=463, Percent_Identity=34.5572354211663, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI45439306, Length=447, Percent_Identity=29.7539149888143, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1787161, Length=448, Percent_Identity=35.2678571428571, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1790571, Length=455, Percent_Identity=23.956043956044, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789256, Length=450, Percent_Identity=24.4444444444444, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788173, Length=246, Percent_Identity=27.2357723577236, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=433, Percent_Identity=38.1062355658199, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17556372, Length=392, Percent_Identity=30.6122448979592, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17551876, Length=324, Percent_Identity=31.1728395061728, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321807, Length=440, Percent_Identity=37.5, Blast_Score=286, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6319872, Length=453, Percent_Identity=32.6710816777042, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323011, Length=470, Percent_Identity=28.5106382978723, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19921528, Length=436, Percent_Identity=36.4678899082569, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI19922416, Length=456, Percent_Identity=32.6754385964912, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17136276, Length=449, Percent_Identity=26.9487750556793, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 49873; Mature: 49873

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKSIAEIMSNDKIGSHVCIRGWIYRRSVVGGKAFIRVRDSSGVIQVVVDSARLGEALV
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCHHHHHHHH
DELKDIGLEASVVACGTVRREERAPGGYELDVDFFKIIGYSRDFPIKGGEGIEYLLDNRH
HHHHHCCCCEEEEEHHHHHHHHCCCCCEEECHHHHHHHCCCCCCCCCCCCCHHEEECCCE
LWVRSPRFTKIFRIKHTILQAGREYFTRNGWWEVNPPILTASACEGGATLFPVEYFESKA
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCEEEEHHHHHHHH
YLSQSAQLYLEALIYVLEKVWSLTPSFRAEKSRTRRHLAEYWHLEPEAAWYSMDDMMKVA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHH
EELASHIVTRVLEERRSELVDLGRDIEKLKPAMETPYPRIRYDEAIEILQKKGVNVKWGD
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHCCCCCCCHHHHHHHHHHCCCCEEECC
DLGADEERILTMEFEKPFFVTHFPRQIKSFYMKVDPSNPNVVLGFDLLAPEGYGEIVGGG
CCCCCCCEEEEEEECCCEEEEHHHHHHHHHEEEECCCCCCEEEEEEEECCCCCCCCCCCC
EREDRYDVLYQKIIEQGLNPEDYKWYLDLRKYGSVPHSGFGLGVERVTMWITGLDHIRDT
CCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHC
LPFPRFRGRIYP
CCCHHHCCCCCC
>Mature Secondary Structure
MEYKSIAEIMSNDKIGSHVCIRGWIYRRSVVGGKAFIRVRDSSGVIQVVVDSARLGEALV
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCHHHHHHHH
DELKDIGLEASVVACGTVRREERAPGGYELDVDFFKIIGYSRDFPIKGGEGIEYLLDNRH
HHHHHCCCCEEEEEHHHHHHHHCCCCCEEECHHHHHHHCCCCCCCCCCCCCHHEEECCCE
LWVRSPRFTKIFRIKHTILQAGREYFTRNGWWEVNPPILTASACEGGATLFPVEYFESKA
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEECCCCCCCEEEEHHHHHHHH
YLSQSAQLYLEALIYVLEKVWSLTPSFRAEKSRTRRHLAEYWHLEPEAAWYSMDDMMKVA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHH
EELASHIVTRVLEERRSELVDLGRDIEKLKPAMETPYPRIRYDEAIEILQKKGVNVKWGD
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHCCCCCCCHHHHHHHHHHCCCCEEECC
DLGADEERILTMEFEKPFFVTHFPRQIKSFYMKVDPSNPNVVLGFDLLAPEGYGEIVGGG
CCCCCCCEEEEEEECCCEEEEHHHHHHHHHEEEECCCCCCEEEEEEEECCCCCCCCCCCC
EREDRYDVLYQKIIEQGLNPEDYKWYLDLRKYGSVPHSGFGLGVERVTMWITGLDHIRDT
CCCHHHHHHHHHHHHCCCCCCCEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHC
LPFPRFRGRIYP
CCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]