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The map label for this gene is nth [H]

Identifier: 218883640

GI number: 218883640

Start: 303332

End: 304048

Strand: Reverse

Name: nth [H]

Synonym: DKAM_0329

Alternate gene names: 218883640

Gene position: 304048-303332 (Counterclockwise)

Preceding gene: 218883641

Following gene: 218883639

Centisome position: 22.27

GC content: 47.98

Gene sequence:

>717_bases
GTGTATATATCGTTGAATACTAGGATCGGGGATGAGGTGCTGAATAGGTTAAGGAAGGAGTATAAGCTGGATGCGGATGA
ATTCGTGGTATCTGAGAGATGTATTCAAAGCAACCGGTTGTTTGAGATAATCACGGCTGTAATTCTAAGCCAGAACACTA
GCGATAGAAATGCATGCAGGGCGTTGCAGAAGCTCAGGGAGTTAACCGGTGGAGTCATCACGCCTGAGACAGTGCTTTTG
CTACCGGTTGATAAACTAGAGGACGCCTTGAGACCAGCGGGTATGTATAGGAATAGGTCTAGGGTGATCAGGGAGCTCGC
CTCCGTGTTTAACCAGGGTGGCTTCCAGGAGAGATTAATCAGTGAGGTGAGTAGGAGTAGTGTAGAGGAGGCACGGAGAC
TACTCATGGAGCTACCTGGTGTCGGCTGGAAAACAGCGGATGTCGTGTTATTAAGGTATTTCAGAAAACCCGTGTTCCCA
GTTGACACGCATATAACCAGGATAACAATGCGGATGGGTTTCACCGGGTCAAGGAGCTATAAGCATATATCGCGGTTCTG
GATGGATAATACATCCCCCGGGAACTACCTGGATCTACACTTGTATCTTATAACACATGGTAGGAGAACATGCAGGGCGC
GGAAACCCCTTTGCAATAAATGCGTCTTGAGAGACATGTGCAAGTATGGGGTTGACACTCTCGATGACAAGAGGTAG

Upstream 100 bases:

>100_bases
GGGTAGAAAGAGTTACGATGTGGATTACTGGGCTGGATCACATAAGGGATACACTACCGTTCCCCAGGTTCAGGGGAAGA
ATCTACCCGTAGGCTAAAGG

Downstream 100 bases:

>100_bases
CTTACTAAAGGAATTAGCGAGAGAGATACTTGGAGAGGGGGTTGCCGAGAAGGTCTGGAGAAGGGTTGAGTTCATAGGCG
ATCTCGCATTAATCAGGACA

Product: Endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MYISLNTRIGDEVLNRLRKEYKLDADEFVVSERCIQSNRLFEIITAVILSQNTSDRNACRALQKLRELTGGVITPETVLL
LPVDKLEDALRPAGMYRNRSRVIRELASVFNQGGFQERLISEVSRSSVEEARRLLMELPGVGWKTADVVLLRYFRKPVFP
VDTHITRITMRMGFTGSRSYKHISRFWMDNTSPGNYLDLHLYLITHGRRTCRARKPLCNKCVLRDMCKYGVDTLDDKR

Sequences:

>Translated_238_residues
MYISLNTRIGDEVLNRLRKEYKLDADEFVVSERCIQSNRLFEIITAVILSQNTSDRNACRALQKLRELTGGVITPETVLL
LPVDKLEDALRPAGMYRNRSRVIRELASVFNQGGFQERLISEVSRSSVEEARRLLMELPGVGWKTADVVLLRYFRKPVFP
VDTHITRITMRMGFTGSRSYKHISRFWMDNTSPGNYLDLHLYLITHGRRTCRARKPLCNKCVLRDMCKYGVDTLDDKR
>Mature_238_residues
MYISLNTRIGDEVLNRLRKEYKLDADEFVVSERCIQSNRLFEIITAVILSQNTSDRNACRALQKLRELTGGVITPETVLL
LPVDKLEDALRPAGMYRNRSRVIRELASVFNQGGFQERLISEVSRSSVEEARRLLMELPGVGWKTADVVLLRYFRKPVFP
VDTHITRITMRMGFTGSRSYKHISRFWMDNTSPGNYLDLHLYLITHGRRTCRARKPLCNKCVLRDMCKYGVDTLDDKR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=218, Percent_Identity=32.1100917431193, Blast_Score=108, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45550361, Length=201, Percent_Identity=23.3830845771144, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27575; Mature: 27575

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYISLNTRIGDEVLNRLRKEYKLDADEFVVSERCIQSNRLFEIITAVILSQNTSDRNACR
CEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHH
ALQKLRELTGGVITPETVLLLPVDKLEDALRPAGMYRNRSRVIRELASVFNQGGFQERLI
HHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHH
SEVSRSSVEEARRLLMELPGVGWKTADVVLLRYFRKPVFPVDTHITRITMRMGFTGSRSY
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
KHISRFWMDNTSPGNYLDLHLYLITHGRRTCRARKPLCNKCVLRDMCKYGVDTLDDKR
HHHHHHHCCCCCCCCEEEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MYISLNTRIGDEVLNRLRKEYKLDADEFVVSERCIQSNRLFEIITAVILSQNTSDRNACR
CEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHH
ALQKLRELTGGVITPETVLLLPVDKLEDALRPAGMYRNRSRVIRELASVFNQGGFQERLI
HHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHH
SEVSRSSVEEARRLLMELPGVGWKTADVVLLRYFRKPVFPVDTHITRITMRMGFTGSRSY
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
KHISRFWMDNTSPGNYLDLHLYLITHGRRTCRARKPLCNKCVLRDMCKYGVDTLDDKR
HHHHHHHCCCCCCCCEEEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]