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The map label for this gene is nfsA [C]

Identifier: 218883582

GI number: 218883582

Start: 248975

End: 249496

Strand: Direct

Name: nfsA [C]

Synonym: DKAM_0271

Alternate gene names: 218883582

Gene position: 248975-249496 (Clockwise)

Preceding gene: 218883577

Following gene: 218883584

Centisome position: 18.24

GC content: 48.85

Gene sequence:

>522_bases
GTGAGCGAGATATTACTTGAGCTACTTAAATCGAGGCGCAGTATAAGAAGGTTCAAGCCTGAACCTCCGCCAAGAGACCT
GGTCTTAAAAGCAATAGATGCAGCCAGGTATGCCCCGAGCGCTAGGAACAGCCAACCCTGGAGATTCATAATAATCGAGG
ACCCGGTTATCAGGCAAAGGCTCGGCGGGATACATATATATGCGAGGCCAGTGTTAAATGCCCCCCTCGCAGTAGTGGTT
GCATGCATGAATGAGGAATCACCAACATCATACCTCCTCGACTGCGCTAACGCAACAATATACTTCATGCTAGCAGCCCA
CGCCCTTGGATTAGGCACAGTATGGATCCAGACACTAAGGAATGTAAAAGAGATCAGGGAGATACTTGGTCTCCCAGACA
ATGTTACCCCGGTTTCCCTACTAGCTGTGGGATACCCTGCTGAAAACCCTGTCCTAAGACCGAGGAAGCCCCTAGAGGAA
GTAGTCTACTTAAACAAGTATGGAGAGCCCTTAAAAATCTAG

Upstream 100 bases:

>100_bases
GATCCAGCTATTATCGCCCAGTATTTAATAGGGTTATTGAACCGTTCAACAATTCCGCTTAAATAGCTTGGAAAACAATA
TTTATCGCTGGTGATCACTG

Downstream 100 bases:

>100_bases
TAGTAAGGTAATGAAGGGGCTTCCTCAGTAACCATTGCTATTTCATCAGCCCTCCTAAGGGATAGAACTGAGTAAATAGT
GATCCCTAATGCTAGGACTC

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSEILLELLKSRRSIRRFKPEPPPRDLVLKAIDAARYAPSARNSQPWRFIIIEDPVIRQRLGGIHIYARPVLNAPLAVVV
ACMNEESPTSYLLDCANATIYFMLAAHALGLGTVWIQTLRNVKEIREILGLPDNVTPVSLLAVGYPAENPVLRPRKPLEE
VVYLNKYGEPLKI

Sequences:

>Translated_173_residues
MSEILLELLKSRRSIRRFKPEPPPRDLVLKAIDAARYAPSARNSQPWRFIIIEDPVIRQRLGGIHIYARPVLNAPLAVVV
ACMNEESPTSYLLDCANATIYFMLAAHALGLGTVWIQTLRNVKEIREILGLPDNVTPVSLLAVGYPAENPVLRPRKPLEE
VVYLNKYGEPLKI
>Mature_172_residues
SEILLELLKSRRSIRRFKPEPPPRDLVLKAIDAARYAPSARNSQPWRFIIIEDPVIRQRLGGIHIYARPVLNAPLAVVVA
CMNEESPTSYLLDCANATIYFMLAAHALGLGTVWIQTLRNVKEIREILGLPDNVTPVSLLAVGYPAENPVLRPRKPLEEV
VYLNKYGEPLKI

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=184, Percent_Identity=33.1521739130435, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 19475; Mature: 19344

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEILLELLKSRRSIRRFKPEPPPRDLVLKAIDAARYAPSARNSQPWRFIIIEDPVIRQR
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHH
LGGIHIYARPVLNAPLAVVVACMNEESPTSYLLDCANATIYFMLAAHALGLGTVWIQTLR
HCCEEEEECCHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVKEIREILGLPDNVTPVSLLAVGYPAENPVLRPRKPLEEVVYLNKYGEPLKI
HHHHHHHHHCCCCCCCHHEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEILLELLKSRRSIRRFKPEPPPRDLVLKAIDAARYAPSARNSQPWRFIIIEDPVIRQR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHH
LGGIHIYARPVLNAPLAVVVACMNEESPTSYLLDCANATIYFMLAAHALGLGTVWIQTLR
HCCEEEEECCHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVKEIREILGLPDNVTPVSLLAVGYPAENPVLRPRKPLEEVVYLNKYGEPLKI
HHHHHHHHHCCCCCCCHHEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]