Click here to switch to the map view.

The map label for this gene is nplT [H]

Identifier: 218883584

GI number: 218883584

Start: 251150

End: 253213

Strand: Direct

Name: nplT [H]

Synonym: DKAM_0273

Alternate gene names: 218883584

Gene position: 251150-253213 (Clockwise)

Preceding gene: 218883582

Following gene: 218883591

Centisome position: 18.4

GC content: 46.03

Gene sequence:

>2064_bases
ATGGAGAAGGTGCTAGCCGTAGTGTTATTCATATTGCTTGTCTTAACACCGGTGTTATTTATTGTTGAAAGCGGCTCTGA
AACGCTGGAAATCTATGCTGGCAAGGGCCCGGGTCTAGTCGTACATGATCCCATAGACCCAGCATATCTTTCAATCGCGG
ATGGTTATTTAATCCCTAGGATTAAAATCCTTAAAGCCCTAGATGTCGCGTCGGGAGTGCTTATAGCTAACGGCGTCGAG
TACCCTCTTAAACTCCAGCTGGAGACGAGTAGTTGGAGGATGTATTATGCCACCGTACCTTTGGATAGGAGTGTAAACGG
GTTAAACTATTACTTCAGACTAACGCTCTCTAATGAAACACTCGTCTACGTGTATAACACGGATACAAGCAGGTTCTACT
ATTTCAACGGTTCAATGCCTTTCAAGCAGGTTGAATGGGTTAGAGATAGGGTGGGCTACCAGATATTCCCTGATAGATTC
TATAATGGGAATCCAAGCAATGATCTAAGGGCTAATTTAACCGATGAACTATGGATAAACGAGGTATCCAAGGGTACACC
AGTTTTCACGAGATGGGATGGACCCGTTACCTCCCTTCATTGCTGTCACCAATACTTCGGAGGAGACTTAAAGGGAATCA
CCGAGAAACTCGACTACCTGAAGGAGCTCGGCATTGGATTAATATATTTAAACCCGATCTTCACCTCGGGTAGTGTGCAC
GGCTATGATGTATACGACTACTACACTGTGGATCCCAAGTTTGGAACGCTGGATGACTTGATGATGCTGTTGAATGAGGC
GCATAAACGCGGTATAAGGGTCATATTCGACTTCGTACCTGATCACGTGGGGCTCGGCTTCTGGGCTTTCCAAGACGTTT
ACAGGAATGGACCCAGTAGCCGTTACTGGAGCTGGTTTATAATCTACAAGTGGCCCTTCAAGCTGGGGGATTCAAGTGCG
TATAAGTGCTGGTGGGGCATAGGTAGTCTGCCACAACTGAATGTATTGAATAAAGAGGTAAGGCAGTACTTGATCAATGT
GGCATTATACTGGCTGAGCATAGGCTTTGATGGCTTAAGGATCGACGCGCCGCTAGACGTGATTGACTCGGAAAACTTCT
TCAGAGAGCTCCGTGAAGCCGTTAAGTCAAGGTATCCCGACGCATACATTGTCGGAGAGATATGGGATTATCGCCCCAAG
TGGTTGAGGGGCGAAGCATTCGACTCCCTGATGAATTATTATCTAGGTAGGAACATATTGCTAAGCTATGCAGGGGGCGC
TTTAAACGGTTACACAGCATCAGCCAGGTTATCCGAGTACTATGCCGGTATAGGTGTCAACGTGGCCGGAATGGGTTTCA
ACATCATTGGATCACATGATACATCAAGGATACTAACTGATCTAGGTGGTGGGGGATTAAGGGATACCCCAAGCAATGAA
TCCATAAAACGGTTAAAGCTACTCTCAACACTCCAATATACGCAGCCAGGCATGCCCGTGGTATTCCAGGGTGATGAGAG
AGGCGTGACCGGGAGGCAGAGCAACTATGATGAACAAAGATATCCTATTCAATGGGATAGATTGAACCAGGAGGTATTCG
AACACTATAAGAAGCTCGGTAAGTTAAAGAACACCATTCCAGCTCTTTCAACAAGCATAATACACGTGTTAAGCACAGAG
GGCAGTATACTCGCATATACAAGAGGCTATAATGACGAAGTACTCGTAGTGGCTAACAATGCCTTAGAACCTATAGCATA
CAAGTTACCCGAAGGTAACTGGTCAATACTCTACGTGAGTAGCGGGGAGAACGTAGAGGTGTATAATGGATCGATCACCG
TGCCCCCGTTAACAGCTATAATATTGGCGAAGGAGCAGTCTTCAACAACTAGTAACGTGACCCAGCCTATTTTCACTCAG
ACAACGACCCCCTCAAATAACACTGGTTTCACCCAGCCTAGTGAGGAATTATGGAGAACCCTCTTCCCTGTATTCACGGC
CGTAACCGTATTAGTGTTCATTACAATGGCTGTCGTTTTAATAAAGCACGTGAAACCACGGTGA

Upstream 100 bases:

>100_bases
AGATTTTACGCATGCGATTACATTCTTCAATAAAAGAATTTAATAGTAAACCCTAGTAAACCTATATGAAAGCAGAAACA
GCATACCGAGGCGTCTAGTC

Downstream 100 bases:

>100_bases
ATAAGGATATTTAGTCCTTATATGGCTCTACATGTATCGTTGCCTCCCACTTCAACTCTTTCCTTATCAACTCCTCTAGT
TTACTAGCTATCTCATGCGC

Product: pullulanase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MEKVLAVVLFILLVLTPVLFIVESGSETLEIYAGKGPGLVVHDPIDPAYLSIADGYLIPRIKILKALDVASGVLIANGVE
YPLKLQLETSSWRMYYATVPLDRSVNGLNYYFRLTLSNETLVYVYNTDTSRFYYFNGSMPFKQVEWVRDRVGYQIFPDRF
YNGNPSNDLRANLTDELWINEVSKGTPVFTRWDGPVTSLHCCHQYFGGDLKGITEKLDYLKELGIGLIYLNPIFTSGSVH
GYDVYDYYTVDPKFGTLDDLMMLLNEAHKRGIRVIFDFVPDHVGLGFWAFQDVYRNGPSSRYWSWFIIYKWPFKLGDSSA
YKCWWGIGSLPQLNVLNKEVRQYLINVALYWLSIGFDGLRIDAPLDVIDSENFFRELREAVKSRYPDAYIVGEIWDYRPK
WLRGEAFDSLMNYYLGRNILLSYAGGALNGYTASARLSEYYAGIGVNVAGMGFNIIGSHDTSRILTDLGGGGLRDTPSNE
SIKRLKLLSTLQYTQPGMPVVFQGDERGVTGRQSNYDEQRYPIQWDRLNQEVFEHYKKLGKLKNTIPALSTSIIHVLSTE
GSILAYTRGYNDEVLVVANNALEPIAYKLPEGNWSILYVSSGENVEVYNGSITVPPLTAIILAKEQSSTTSNVTQPIFTQ
TTTPSNNTGFTQPSEELWRTLFPVFTAVTVLVFITMAVVLIKHVKPR

Sequences:

>Translated_687_residues
MEKVLAVVLFILLVLTPVLFIVESGSETLEIYAGKGPGLVVHDPIDPAYLSIADGYLIPRIKILKALDVASGVLIANGVE
YPLKLQLETSSWRMYYATVPLDRSVNGLNYYFRLTLSNETLVYVYNTDTSRFYYFNGSMPFKQVEWVRDRVGYQIFPDRF
YNGNPSNDLRANLTDELWINEVSKGTPVFTRWDGPVTSLHCCHQYFGGDLKGITEKLDYLKELGIGLIYLNPIFTSGSVH
GYDVYDYYTVDPKFGTLDDLMMLLNEAHKRGIRVIFDFVPDHVGLGFWAFQDVYRNGPSSRYWSWFIIYKWPFKLGDSSA
YKCWWGIGSLPQLNVLNKEVRQYLINVALYWLSIGFDGLRIDAPLDVIDSENFFRELREAVKSRYPDAYIVGEIWDYRPK
WLRGEAFDSLMNYYLGRNILLSYAGGALNGYTASARLSEYYAGIGVNVAGMGFNIIGSHDTSRILTDLGGGGLRDTPSNE
SIKRLKLLSTLQYTQPGMPVVFQGDERGVTGRQSNYDEQRYPIQWDRLNQEVFEHYKKLGKLKNTIPALSTSIIHVLSTE
GSILAYTRGYNDEVLVVANNALEPIAYKLPEGNWSILYVSSGENVEVYNGSITVPPLTAIILAKEQSSTTSNVTQPIFTQ
TTTPSNNTGFTQPSEELWRTLFPVFTAVTVLVFITMAVVLIKHVKPR
>Mature_687_residues
MEKVLAVVLFILLVLTPVLFIVESGSETLEIYAGKGPGLVVHDPIDPAYLSIADGYLIPRIKILKALDVASGVLIANGVE
YPLKLQLETSSWRMYYATVPLDRSVNGLNYYFRLTLSNETLVYVYNTDTSRFYYFNGSMPFKQVEWVRDRVGYQIFPDRF
YNGNPSNDLRANLTDELWINEVSKGTPVFTRWDGPVTSLHCCHQYFGGDLKGITEKLDYLKELGIGLIYLNPIFTSGSVH
GYDVYDYYTVDPKFGTLDDLMMLLNEAHKRGIRVIFDFVPDHVGLGFWAFQDVYRNGPSSRYWSWFIIYKWPFKLGDSSA
YKCWWGIGSLPQLNVLNKEVRQYLINVALYWLSIGFDGLRIDAPLDVIDSENFFRELREAVKSRYPDAYIVGEIWDYRPK
WLRGEAFDSLMNYYLGRNILLSYAGGALNGYTASARLSEYYAGIGVNVAGMGFNIIGSHDTSRILTDLGGGGLRDTPSNE
SIKRLKLLSTLQYTQPGMPVVFQGDERGVTGRQSNYDEQRYPIQWDRLNQEVFEHYKKLGKLKNTIPALSTSIIHVLSTE
GSILAYTRGYNDEVLVVANNALEPIAYKLPEGNWSILYVSSGENVEVYNGSITVPPLTAIILAKEQSSTTSNVTQPIFTQ
TTTPSNNTGFTQPSEELWRTLFPVFTAVTVLVFITMAVVLIKHVKPR

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=202, Percent_Identity=28.7128712871287, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI1786604, Length=523, Percent_Identity=29.2543021032505, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1790687, Length=352, Percent_Identity=27.8409090909091, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789995, Length=557, Percent_Identity=22.8007181328546, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322245, Length=180, Percent_Identity=32.7777777777778, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321726, Length=372, Percent_Identity=26.3440860215054, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322241, Length=180, Percent_Identity=30, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322021, Length=180, Percent_Identity=30, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324416, Length=180, Percent_Identity=30, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321731, Length=181, Percent_Identity=29.2817679558011, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319776, Length=181, Percent_Identity=29.2817679558011, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24586587, Length=399, Percent_Identity=26.0651629072682, Blast_Score=109, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24586593, Length=414, Percent_Identity=27.7777777777778, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI221330053, Length=395, Percent_Identity=25.5696202531646, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24586591, Length=403, Percent_Identity=25.0620347394541, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45549022, Length=376, Percent_Identity=26.3297872340426, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24586589, Length=403, Percent_Identity=28.287841191067, Blast_Score=97, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24583747, Length=191, Percent_Identity=34.5549738219895, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24583749, Length=191, Percent_Identity=34.5549738219895, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24586597, Length=399, Percent_Identity=25.8145363408521, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24583745, Length=370, Percent_Identity=25.4054054054054, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24586599, Length=403, Percent_Identity=24.0694789081886, Blast_Score=91, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 77760; Mature: 77760

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVLAVVLFILLVLTPVLFIVESGSETLEIYAGKGPGLVVHDPIDPAYLSIADGYLIPR
CHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCEEEECCCCCCEEEEECCCCCCH
IKILKALDVASGVLIANGVEYPLKLQLETSSWRMYYATVPLDRSVNGLNYYFRLTLSNET
HHHHHHHHHHCCEEEECCCCCCEEEEEECCCEEEEEEEECCCCCCCCEEEEEEEEECCCE
LVYVYNTDTSRFYYFNGSMPFKQVEWVRDRVGYQIFPDRFYNGNPSNDLRANLTDELWIN
EEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHH
EVSKGTPVFTRWDGPVTSLHCCHQYFGGDLKGITEKLDYLKELGIGLIYLNPIFTSGSVH
HHCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
GYDVYDYYTVDPKFGTLDDLMMLLNEAHKRGIRVIFDFVPDHVGLGFWAFQDVYRNGPSS
CEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEECCCHHCCHHHHHHHHHHCCCCC
RYWSWFIIYKWPFKLGDSSAYKCWWGIGSLPQLNVLNKEVRQYLINVALYWLSIGFDGLR
CEEEEEEEEEECEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
IDAPLDVIDSENFFRELREAVKSRYPDAYIVGEIWDYRPKWLRGEAFDSLMNYYLGRNIL
ECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHCCCCCCCCCHHHHHHHHHHHCCCEE
LSYAGGALNGYTASARLSEYYAGIGVNVAGMGFNIIGSHDTSRILTDLGGGGLRDTPSNE
EEECCCCCCCCCHHHHHHHHHCCCCEEEECCCEEEECCCCHHHHHHHCCCCCCCCCCCCH
SIKRLKLLSTLQYTQPGMPVVFQGDERGVTGRQSNYDEQRYPIQWDRLNQEVFEHYKKLG
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHH
KLKNTIPALSTSIIHVLSTEGSILAYTRGYNDEVLVVANNALEPIAYKLPEGNWSILYVS
HHHHHHHHHHHHHEEEEECCCCEEEEECCCCCCEEEEECCCCCCEEEECCCCCEEEEEEE
SGENVEVYNGSITVPPLTAIILAKEQSSTTSNVTQPIFTQTTTPSNNTGFTQPSEELWRT
CCCEEEEEECEEECCCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
LFPVFTAVTVLVFITMAVVLIKHVKPR
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKVLAVVLFILLVLTPVLFIVESGSETLEIYAGKGPGLVVHDPIDPAYLSIADGYLIPR
CHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCEEEECCCCCCEEEEECCCCCCH
IKILKALDVASGVLIANGVEYPLKLQLETSSWRMYYATVPLDRSVNGLNYYFRLTLSNET
HHHHHHHHHHCCEEEECCCCCCEEEEEECCCEEEEEEEECCCCCCCCEEEEEEEEECCCE
LVYVYNTDTSRFYYFNGSMPFKQVEWVRDRVGYQIFPDRFYNGNPSNDLRANLTDELWIN
EEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHH
EVSKGTPVFTRWDGPVTSLHCCHQYFGGDLKGITEKLDYLKELGIGLIYLNPIFTSGSVH
HHCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
GYDVYDYYTVDPKFGTLDDLMMLLNEAHKRGIRVIFDFVPDHVGLGFWAFQDVYRNGPSS
CEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEEECCCHHCCHHHHHHHHHHCCCCC
RYWSWFIIYKWPFKLGDSSAYKCWWGIGSLPQLNVLNKEVRQYLINVALYWLSIGFDGLR
CEEEEEEEEEECEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
IDAPLDVIDSENFFRELREAVKSRYPDAYIVGEIWDYRPKWLRGEAFDSLMNYYLGRNIL
ECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECHHCCCCCCCCCHHHHHHHHHHHCCCEE
LSYAGGALNGYTASARLSEYYAGIGVNVAGMGFNIIGSHDTSRILTDLGGGGLRDTPSNE
EEECCCCCCCCCHHHHHHHHHCCCCEEEECCCEEEECCCCHHHHHHHCCCCCCCCCCCCH
SIKRLKLLSTLQYTQPGMPVVFQGDERGVTGRQSNYDEQRYPIQWDRLNQEVFEHYKKLG
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHH
KLKNTIPALSTSIIHVLSTEGSILAYTRGYNDEVLVVANNALEPIAYKLPEGNWSILYVS
HHHHHHHHHHHHHEEEEECCCCEEEEECCCCCCEEEEECCCCCCEEEECCCCCEEEEEEE
SGENVEVYNGSITVPPLTAIILAKEQSSTTSNVTQPIFTQTTTPSNNTGFTQPSEELWRT
CCCEEEEEECEEECCCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHH
LFPVFTAVTVLVFITMAVVLIKHVKPR
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]