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The map label for this gene is ligA

Identifier: 218532981

GI number: 218532981

Start: 5471890

End: 5474334

Strand: Reverse

Name: ligA

Synonym: Mchl_5105

Alternate gene names: 218532981

Gene position: 5474334-5471890 (Counterclockwise)

Preceding gene: 218532985

Following gene: 218532980

Centisome position: 94.75

GC content: 70.27

Gene sequence:

>2445_bases
ATGCCGAGCACGCCCCTTTCCGCCACCGTCGAAGCCTTGAGCGAGGATGCCGCCCAGGCGCGTCATGCCGAGCTATCGAG
GGCGATCGAGCGGGCGAACCAGCTCTACTACAACGAGGACGCGCCCGAACTCACCGACGCCGAATATGACGCCCTGCGCC
AGGAGCTGGAGGCGATCGAAGCGCGCTTTCCCGCGCTGACCGGCACCACCGAGGCGAGCGCGGGCGTCGGCGCCAAGCCT
TCGGAAAAGTTCGCCAAGGTCCGGCACGCGGTGCCGATGCTCTCGCTCGGCAACGCCTTCGCCGACGAAGACGTGGACGA
GTTCGTCGCCCGCGTGCGTCGCTTTCTCAACCTGCCGGCTGAGGAGGCGGTCGCCTTCACCGCCGAGCCGAAGATCGACG
GGCTGTCGCTGTCGCTGCGTTACGAGGCGGGCCGGCTCGTCACCGCGGCGACGCGGGGCGACGGCGAGGTTGGCGAGAAC
GTCACCGCCAACGCGCTGACGGTGGACGACATCCCGGAAACGCTCTCCGGCGAGGGCATCCCCGAGGTGTTGGAGGTGCG
CGGCGAGATCTACCTCTCACACGAGGATTTCGCCGCGATCAACGCGCGCCAGGAGGCGGCGGGCAAGCCGCTCTTCGCCA
ATCCGCGCAACGCCGCGGCCGGTTCGCTGCGCCAGCTCGATCCGGCGATCACCGCCTCACGGCCCTTGCGCTTCTTCGCC
TATGCCTGGGGCGAGGTCTCCGAGCCCTTCACCGACACGCAATCGGCGGTGCTGGAGCGGTTCCGCGGCTGGGGCCTGCC
GGTGAACCCGCGGACCAAGCTGTGCCGCTCGGCCGAGGAGATGATCGCGCATTACCGCGCCATCGAGGCGGAGCGGGCGG
GTCTCGGCTACGACATCGACGGCGTCGTCTACAAAGTGGACGACCTTGGCTTCCAGCGCCGGCTCGGCTTCGTCAGCCGC
GCGCCGCGCTGGGCGCTGGCGCACAAATTCGCGGCGCAGGAGGCGATCACCGAACTTCTCGCCATCGACATCAATGTCGG
CCGAACCGGCTCGCTCAACCCGCTGGCGCGCCTCAAGCCCGTCACCGTCGGCGGCGTCGTGGTCTCGAACGCGACTTTGC
ACAACGAGGGCTACGTCCAGGGCGTGGGCGCCGACGGCGAGCCGATCCGCGAGGGTCGCGACATCCGCGTCGGCGACACC
GTGATCGTGGTGCGCGCCGGCGACGTCATCCCGAAGGTGCGGGATGTGGTGATCGAGAAGCGCCCGGCCGACGCCGTTCC
CTACGTCTTCCCGGATACCTGCCCGGCCTGCGGCAGCCGGGCCGTGCGTGAACTCAACCCGCGTACGAAAAAACTCGATG
CGATCCGCCGTTGTACCGGCGGGTTGATCTGCCCGGCGCAGGGCGTGGAGCGGCTGAAGCACTTCGTTTCGCGCAACGGC
CTCGACCTCGAAGGCTTCGGCCAGACCTATATCGAGGTGCTGTTCGAGGCCGGCCTCGTGAAGCAGCCCGCCGACCTGTT
CCGCCTGGAGTTCGAGCCGCTGAAGGCGGCCATCGTCGCCCGGCGCGAGGCCCTCTCCGCCCAGCGCCGTACCGAGGGCG
AGCCGGCGCCGAAGAAGCCGACCAAGAAGAAGGGTGAGGAGGAGGACAAGGCGATCAAGAACCTGCTCGCTTCGCTCGAC
GCCCGCCGAACGATCCCCCTCAACCGCTTCCTGTTCGCCCTCGGCATCCCGCAGATCGGCGAATCGACGGCCAAGGCGCT
GGCTAAGCGCTTCCCCGACATGCCCGCCCTGATGGCGGCCCTCGCAGCGGCGACGCGGGAGCAGGCGGGCCGCGACTGGC
TCGAACTCTCGGCGCTGCCGCGGATCGGGCCCGGCACCCGCGACCGGCTGTTCGAGACCCTCGACCCGCTGCCCGGCGAG
GCGATGCAGGATCTGAGCCTCGGCGCCCGCCTGCGGGGGCGGCTCACGCCGTCGCAGCGCGAGGCGGTGCTCGCCCATTA
CGGCAGCGAGGCGGAGGCCGACGCGGCGCTGGAGCGGGCGGCCTCGCAGCGGCCCGGCGACGCCTACCGTCTGTTCGCCG
ACGACGGCGAGATCGGCCCCGTGGCGACGGATTCGCTGATCCAGTTCTTCTCCGAGCCGCACAACGACGCGGCGGTGCGG
GCGCTGCTCGAAGAGGTGGGAACCGAGCCGCTGGCCGCCACGACCTCGGCGGCGGCGTTTGCCGGCAAGACGGTGGTGTT
CACCGGCTCCCTCGAAAAGATGACCCGCAGCGAGGCCAAGGCCACCGCCGAGCGCCTGGGCGCCAAGGTCTCCGGCTCGG
TCTCGGCCAAGACCGACCTCGTGGTCGCCGGCCCCGGCGCCGGCTCGAAGCTCAAGGACGCCGAGAAGCACGGGGTGAAG
GTGGTCTCGGAGGACGATTGGCTGGCGATGCTGGCGGAGGCGTAA

Upstream 100 bases:

>100_bases
TCGTTTCGAGGGTTCTGCCCGTCGAGTTGTGGCGGGAATGGGGACGTTGCCGGCACCAGCGTTAACGCACCGTCGCGCGA
GATCGGTTAGAACGCGGCCC

Downstream 100 bases:

>100_bases
AGTTTCCCCCGAGCCTTGACCCCGCGCCCCCCGCGGCGCAATCCGTGCCCCGGATCAGCCGGCCGGGCAGCCGCTCCGTC
GTCAGACGGGGAGGAAAGTC

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 814; Mature: 813

Protein sequence:

>814_residues
MPSTPLSATVEALSEDAAQARHAELSRAIERANQLYYNEDAPELTDAEYDALRQELEAIEARFPALTGTTEASAGVGAKP
SEKFAKVRHAVPMLSLGNAFADEDVDEFVARVRRFLNLPAEEAVAFTAEPKIDGLSLSLRYEAGRLVTAATRGDGEVGEN
VTANALTVDDIPETLSGEGIPEVLEVRGEIYLSHEDFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFA
YAWGEVSEPFTDTQSAVLERFRGWGLPVNPRTKLCRSAEEMIAHYRAIEAERAGLGYDIDGVVYKVDDLGFQRRLGFVSR
APRWALAHKFAAQEAITELLAIDINVGRTGSLNPLARLKPVTVGGVVVSNATLHNEGYVQGVGADGEPIREGRDIRVGDT
VIVVRAGDVIPKVRDVVIEKRPADAVPYVFPDTCPACGSRAVRELNPRTKKLDAIRRCTGGLICPAQGVERLKHFVSRNG
LDLEGFGQTYIEVLFEAGLVKQPADLFRLEFEPLKAAIVARREALSAQRRTEGEPAPKKPTKKKGEEEDKAIKNLLASLD
ARRTIPLNRFLFALGIPQIGESTAKALAKRFPDMPALMAALAAATREQAGRDWLELSALPRIGPGTRDRLFETLDPLPGE
AMQDLSLGARLRGRLTPSQREAVLAHYGSEAEADAALERAASQRPGDAYRLFADDGEIGPVATDSLIQFFSEPHNDAAVR
ALLEEVGTEPLAATTSAAAFAGKTVVFTGSLEKMTRSEAKATAERLGAKVSGSVSAKTDLVVAGPGAGSKLKDAEKHGVK
VVSEDDWLAMLAEA

Sequences:

>Translated_814_residues
MPSTPLSATVEALSEDAAQARHAELSRAIERANQLYYNEDAPELTDAEYDALRQELEAIEARFPALTGTTEASAGVGAKP
SEKFAKVRHAVPMLSLGNAFADEDVDEFVARVRRFLNLPAEEAVAFTAEPKIDGLSLSLRYEAGRLVTAATRGDGEVGEN
VTANALTVDDIPETLSGEGIPEVLEVRGEIYLSHEDFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFA
YAWGEVSEPFTDTQSAVLERFRGWGLPVNPRTKLCRSAEEMIAHYRAIEAERAGLGYDIDGVVYKVDDLGFQRRLGFVSR
APRWALAHKFAAQEAITELLAIDINVGRTGSLNPLARLKPVTVGGVVVSNATLHNEGYVQGVGADGEPIREGRDIRVGDT
VIVVRAGDVIPKVRDVVIEKRPADAVPYVFPDTCPACGSRAVRELNPRTKKLDAIRRCTGGLICPAQGVERLKHFVSRNG
LDLEGFGQTYIEVLFEAGLVKQPADLFRLEFEPLKAAIVARREALSAQRRTEGEPAPKKPTKKKGEEEDKAIKNLLASLD
ARRTIPLNRFLFALGIPQIGESTAKALAKRFPDMPALMAALAAATREQAGRDWLELSALPRIGPGTRDRLFETLDPLPGE
AMQDLSLGARLRGRLTPSQREAVLAHYGSEAEADAALERAASQRPGDAYRLFADDGEIGPVATDSLIQFFSEPHNDAAVR
ALLEEVGTEPLAATTSAAAFAGKTVVFTGSLEKMTRSEAKATAERLGAKVSGSVSAKTDLVVAGPGAGSKLKDAEKHGVK
VVSEDDWLAMLAEA
>Mature_813_residues
PSTPLSATVEALSEDAAQARHAELSRAIERANQLYYNEDAPELTDAEYDALRQELEAIEARFPALTGTTEASAGVGAKPS
EKFAKVRHAVPMLSLGNAFADEDVDEFVARVRRFLNLPAEEAVAFTAEPKIDGLSLSLRYEAGRLVTAATRGDGEVGENV
TANALTVDDIPETLSGEGIPEVLEVRGEIYLSHEDFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFAY
AWGEVSEPFTDTQSAVLERFRGWGLPVNPRTKLCRSAEEMIAHYRAIEAERAGLGYDIDGVVYKVDDLGFQRRLGFVSRA
PRWALAHKFAAQEAITELLAIDINVGRTGSLNPLARLKPVTVGGVVVSNATLHNEGYVQGVGADGEPIREGRDIRVGDTV
IVVRAGDVIPKVRDVVIEKRPADAVPYVFPDTCPACGSRAVRELNPRTKKLDAIRRCTGGLICPAQGVERLKHFVSRNGL
DLEGFGQTYIEVLFEAGLVKQPADLFRLEFEPLKAAIVARREALSAQRRTEGEPAPKKPTKKKGEEEDKAIKNLLASLDA
RRTIPLNRFLFALGIPQIGESTAKALAKRFPDMPALMAALAAATREQAGRDWLELSALPRIGPGTRDRLFETLDPLPGEA
MQDLSLGARLRGRLTPSQREAVLAHYGSEAEADAALERAASQRPGDAYRLFADDGEIGPVATDSLIQFFSEPHNDAAVRA
LLEEVGTEPLAATTSAAAFAGKTVVFTGSLEKMTRSEAKATAERLGAKVSGSVSAKTDLVVAGPGAGSKLKDAEKHGVKV
VSEDDWLAMLAEA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=586, Percent_Identity=45.0511945392491, Blast_Score=467, Evalue=1e-132,
Organism=Escherichia coli, GI87082305, Length=575, Percent_Identity=23.1304347826087, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_METC4 (B7KU78)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002423797.1
- ProteinModelPortal:   B7KU78
- GeneID:   7118980
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_5105
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2332283
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 87642; Mature: 87511

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 131-131 BINDING 129-129 BINDING 152-152 BINDING 189-189 BINDING 305-305 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTPLSATVEALSEDAAQARHAELSRAIERANQLYYNEDAPELTDAEYDALRQELEAIE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHH
ARFPALTGTTEASAGVGAKPSEKFAKVRHAVPMLSLGNAFADEDVDEFVARVRRFLNLPA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCH
EEAVAFTAEPKIDGLSLSLRYEAGRLVTAATRGDGEVGENVTANALTVDDIPETLSGEGI
HHHEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCEEECCCCCHHCCCCCC
PEVLEVRGEIYLSHEDFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFA
HHHHHCCCCEEECCCCHHHHCCHHHHCCCCCCCCCCCHHHCCHHHCCCHHHCCCCHHEEH
YAWGEVSEPFTDTQSAVLERFRGWGLPVNPRTKLCRSAEEMIAHYRAIEAERAGLGYDID
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GVVYKVDDLGFQRRLGFVSRAPRWALAHKFAAQEAITELLAIDINVGRTGSLNPLARLKP
CEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHCCC
VTVGGVVVSNATLHNEGYVQGVGADGEPIREGRDIRVGDTVIVVRAGDVIPKVRDVVIEK
EEECCEEEECCEECCCCCEEEECCCCCCCCCCCCEEECCEEEEEECCCCCHHHHHHHHCC
RPADAVPYVFPDTCPACGSRAVRELNPRTKKLDAIRRCTGGLICPAQGVERLKHFVSRNG
CCCCCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
LDLEGFGQTYIEVLFEAGLVKQPADLFRLEFEPLKAAIVARREALSAQRRTEGEPAPKKP
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TKKKGEEEDKAIKNLLASLDARRTIPLNRFLFALGIPQIGESTAKALAKRFPDMPALMAA
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHH
LAAATREQAGRDWLELSALPRIGPGTRDRLFETLDPLPGEAMQDLSLGARLRGRLTPSQR
HHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCHHHHCCCCCHHH
EAVLAHYGSEAEADAALERAASQRPGDAYRLFADDGEIGPVATDSLIQFFSEPHNDAAVR
HHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHH
ALLEEVGTEPLAATTSAAAFAGKTVVFTGSLEKMTRSEAKATAERLGAKVSGSVSAKTDL
HHHHHHCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
VVAGPGAGSKLKDAEKHGVKVVSEDDWLAMLAEA
EEECCCCCCCCCHHHHCCCEEECCCCHHHHCCCC
>Mature Secondary Structure 
PSTPLSATVEALSEDAAQARHAELSRAIERANQLYYNEDAPELTDAEYDALRQELEAIE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHH
ARFPALTGTTEASAGVGAKPSEKFAKVRHAVPMLSLGNAFADEDVDEFVARVRRFLNLPA
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCH
EEAVAFTAEPKIDGLSLSLRYEAGRLVTAATRGDGEVGENVTANALTVDDIPETLSGEGI
HHHEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCEEECCCCCHHCCCCCC
PEVLEVRGEIYLSHEDFAAINARQEAAGKPLFANPRNAAAGSLRQLDPAITASRPLRFFA
HHHHHCCCCEEECCCCHHHHCCHHHHCCCCCCCCCCCHHHCCHHHCCCHHHCCCCHHEEH
YAWGEVSEPFTDTQSAVLERFRGWGLPVNPRTKLCRSAEEMIAHYRAIEAERAGLGYDID
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GVVYKVDDLGFQRRLGFVSRAPRWALAHKFAAQEAITELLAIDINVGRTGSLNPLARLKP
CEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHCCC
VTVGGVVVSNATLHNEGYVQGVGADGEPIREGRDIRVGDTVIVVRAGDVIPKVRDVVIEK
EEECCEEEECCEECCCCCEEEECCCCCCCCCCCCEEECCEEEEEECCCCCHHHHHHHHCC
RPADAVPYVFPDTCPACGSRAVRELNPRTKKLDAIRRCTGGLICPAQGVERLKHFVSRNG
CCCCCCCEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
LDLEGFGQTYIEVLFEAGLVKQPADLFRLEFEPLKAAIVARREALSAQRRTEGEPAPKKP
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TKKKGEEEDKAIKNLLASLDARRTIPLNRFLFALGIPQIGESTAKALAKRFPDMPALMAA
CCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHH
LAAATREQAGRDWLELSALPRIGPGTRDRLFETLDPLPGEAMQDLSLGARLRGRLTPSQR
HHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCHHHHCCCCCHHH
EAVLAHYGSEAEADAALERAASQRPGDAYRLFADDGEIGPVATDSLIQFFSEPHNDAAVR
HHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHH
ALLEEVGTEPLAATTSAAAFAGKTVVFTGSLEKMTRSEAKATAERLGAKVSGSVSAKTDL
HHHHHHCCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
VVAGPGAGSKLKDAEKHGVKVVSEDDWLAMLAEA
EEECCCCCCCCCHHHHCCCEEECCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA