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The map label for this gene is murE [H]

Identifier: 218532977

GI number: 218532977

Start: 5466075

End: 5467553

Strand: Reverse

Name: murE [H]

Synonym: Mchl_5101

Alternate gene names: 218532977

Gene position: 5467553-5466075 (Counterclockwise)

Preceding gene: 218532978

Following gene: 218532976

Centisome position: 94.63

GC content: 72.55

Gene sequence:

>1479_bases
ATGAGCCAGCCGACGCCTGACGACCGGACTCTCGGCGCGCTGTTCCCGGAGGTGGACGCGGCCATCGCCGGCCGCCCCGT
CGCGGGCCTCACCGCCGACAGCCGTAAGGTCGTGCCCGGCGGCGTGTTCGTCGCGGTACCCGGCACCGCCGCCGATGGCC
GGCTCTTCGCCGCCGCCGCAGCAGCGGCCGGCGCGGTCGCCGTGGCCGGTGAGGGGACGCGCCCGGAAGATCTGCCCGAG
GCGACGGCCTGGATCGAGGTGGCGGATGCCCGCCGGGCGCTGGCGCTTGCCGCCGCCCGCCTGTCCGGGCGCCAGCCGGA
AACGGTGGTCGCCGTCACCGGCACCAGCGGCAAAAGCTCGGTCGCCGACTTCGTGCGGCAGATCCTGTCGCGCCTCGGCC
GGGACTCGGCGAGTCTCGGCACCGTCGGCATCGTCACCAACCGGGGCGCGCAATACGGCTCGCTGACGACCCCGGACCCC
GTCACGCTGCACGAGACCCTGGCGCGGCTGGCGGACGAGGGCATCACCGATCTGGCCATGGAGGCCTCCTCCCACGGCAT
CGAGCAGCGCCGCCTCGACGGGGTGGAACTGACGGCCGCCGCCTTCACCAATCTCGGCCGCGACCACCTCGATTATCACC
CCACCATCGAGGACTATCTGGACGCCAAGCTCCGCCTGTTCACGACGCTGCTGCCGAAGGGCTGTCCGGCCATCGTCAAC
GCGGACGGTGCCTATGCCGAGCGCGTCATCGGCGTGGCCGACGCCGCCGGCCACGCTATCCGCACCACGGGGCGCGGCGG
CGACTTCATCCGCCTCGTCGAGGCCGGGACGGAGGGTTTCGCGCAGAGCCTGACGCTCTCCCACGCAGGCAAGGACTATC
GCGTGCGCCTGCCGCTGGTCGGTGGCTTTCAGGTCGAGAACGCTCTGGTCGCGGCGGGTCTCGCCCTGGCGACGCCTGCA
GGTCAGGCCGATCCGGCCGGCGTCTTCGCGGCGCTCGAAGGGCTCACCGGCGTGCCGGGCCGCATGGAGCGGATCGGCGA
AGCGAACGGGGGCTTGTGCCTCGTCGATTACGCCCACAAGCCGGAAGCCCTGGAGCATGTCTTGAGCGCGCTCCGCCCCT
TCGCCAGCGGGCGCCTCGTCGTCGTGTTCGGCTGCGGCGGCAATCGCGACGCCGGCAAGCGGCCGATCATGGGCGCCATC
GCCGAACGCCTCGCCGACCGGGTCATCGTCACCGACGACAACCCCCGCAACGAGGAGCCCGCCGCGATCCGCGCCGCGAT
CCTCGCCGCGGCACCGGGCGCGGAGGAGATCGGCGACCGGGCCGAAGCAATCCGCACGGGCGTACGGGACCTGAGAGCCG
GCGACGTGCTGGTGGTGGCCGGCAAAGGACATGAAACCGGCCAGATCGTCGGGCCACGCGTGCTCCCGTTCTCGGATCAC
GACACCCTGCGCGCCGCGATCGCGGAGTTGAACGGATGA

Upstream 100 bases:

>100_bases
AGCTCGGCGCCTACCATGCGACCATGGTGGACGGGCAGTGACGGCAGCCTGTACCCCGTCGGCCTGACCCGCACGGACGC
TTCCGAGGATTGTTTTGCCG

Downstream 100 bases:

>100_bases
GCGACGCCCCCCTCTGGACCCCCGCCGCCCTGGAAGCGGCCACCGGCGGGCGCCTGATCGGTGCGCCGCGCGCCGTCACC
GGCGCCTCGATCGACACCCG

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSQPTPDDRTLGALFPEVDAAIAGRPVAGLTADSRKVVPGGVFVAVPGTAADGRLFAAAAAAAGAVAVAGEGTRPEDLPE
ATAWIEVADARRALALAAARLSGRQPETVVAVTGTSGKSSVADFVRQILSRLGRDSASLGTVGIVTNRGAQYGSLTTPDP
VTLHETLARLADEGITDLAMEASSHGIEQRRLDGVELTAAAFTNLGRDHLDYHPTIEDYLDAKLRLFTTLLPKGCPAIVN
ADGAYAERVIGVADAAGHAIRTTGRGGDFIRLVEAGTEGFAQSLTLSHAGKDYRVRLPLVGGFQVENALVAAGLALATPA
GQADPAGVFAALEGLTGVPGRMERIGEANGGLCLVDYAHKPEALEHVLSALRPFASGRLVVVFGCGGNRDAGKRPIMGAI
AERLADRVIVTDDNPRNEEPAAIRAAILAAAPGAEEIGDRAEAIRTGVRDLRAGDVLVVAGKGHETGQIVGPRVLPFSDH
DTLRAAIAELNG

Sequences:

>Translated_492_residues
MSQPTPDDRTLGALFPEVDAAIAGRPVAGLTADSRKVVPGGVFVAVPGTAADGRLFAAAAAAAGAVAVAGEGTRPEDLPE
ATAWIEVADARRALALAAARLSGRQPETVVAVTGTSGKSSVADFVRQILSRLGRDSASLGTVGIVTNRGAQYGSLTTPDP
VTLHETLARLADEGITDLAMEASSHGIEQRRLDGVELTAAAFTNLGRDHLDYHPTIEDYLDAKLRLFTTLLPKGCPAIVN
ADGAYAERVIGVADAAGHAIRTTGRGGDFIRLVEAGTEGFAQSLTLSHAGKDYRVRLPLVGGFQVENALVAAGLALATPA
GQADPAGVFAALEGLTGVPGRMERIGEANGGLCLVDYAHKPEALEHVLSALRPFASGRLVVVFGCGGNRDAGKRPIMGAI
AERLADRVIVTDDNPRNEEPAAIRAAILAAAPGAEEIGDRAEAIRTGVRDLRAGDVLVVAGKGHETGQIVGPRVLPFSDH
DTLRAAIAELNG
>Mature_491_residues
SQPTPDDRTLGALFPEVDAAIAGRPVAGLTADSRKVVPGGVFVAVPGTAADGRLFAAAAAAAGAVAVAGEGTRPEDLPEA
TAWIEVADARRALALAAARLSGRQPETVVAVTGTSGKSSVADFVRQILSRLGRDSASLGTVGIVTNRGAQYGSLTTPDPV
TLHETLARLADEGITDLAMEASSHGIEQRRLDGVELTAAAFTNLGRDHLDYHPTIEDYLDAKLRLFTTLLPKGCPAIVNA
DGAYAERVIGVADAAGHAIRTTGRGGDFIRLVEAGTEGFAQSLTLSHAGKDYRVRLPLVGGFQVENALVAAGLALATPAG
QADPAGVFAALEGLTGVPGRMERIGEANGGLCLVDYAHKPEALEHVLSALRPFASGRLVVVFGCGGNRDAGKRPIMGAIA
ERLADRVIVTDDNPRNEEPAAIRAAILAAAPGAEEIGDRAEAIRTGVRDLRAGDVLVVAGKGHETGQIVGPRVLPFSDHD
TLRAAIAELNG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=500, Percent_Identity=37.4, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786274, Length=118, Percent_Identity=38.9830508474576, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50645; Mature: 50514

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPTPDDRTLGALFPEVDAAIAGRPVAGLTADSRKVVPGGVFVAVPGTAADGRLFAAAA
CCCCCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCHHHHHH
AAAGAVAVAGEGTRPEDLPEATAWIEVADARRALALAAARLSGRQPETVVAVTGTSGKSS
HHCCEEEEECCCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHH
VADFVRQILSRLGRDSASLGTVGIVTNRGAQYGSLTTPDPVTLHETLARLADEGITDLAM
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHH
EASSHGIEQRRLDGVELTAAAFTNLGRDHLDYHPTIEDYLDAKLRLFTTLLPKGCPAIVN
HHHHCCCHHHHCCCCEEHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
ADGAYAERVIGVADAAGHAIRTTGRGGDFIRLVEAGTEGFAQSLTLSHAGKDYRVRLPLV
CCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEE
GGFQVENALVAAGLALATPAGQADPAGVFAALEGLTGVPGRMERIGEANGGLCLVDYAHK
CCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCC
PEALEHVLSALRPFASGRLVVVFGCGGNRDAGKRPIMGAIAERLADRVIVTDDNPRNEEP
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCC
AAIRAAILAAAPGAEEIGDRAEAIRTGVRDLRAGDVLVVAGKGHETGQIVGPRVLPFSDH
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCEEECCCCC
DTLRAAIAELNG
HHHHHHHHHCCC
>Mature Secondary Structure 
SQPTPDDRTLGALFPEVDAAIAGRPVAGLTADSRKVVPGGVFVAVPGTAADGRLFAAAA
CCCCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCEECCCEEEEEECCCCCCCCHHHHHH
AAAGAVAVAGEGTRPEDLPEATAWIEVADARRALALAAARLSGRQPETVVAVTGTSGKSS
HHCCEEEEECCCCCCCCCCCHHHHEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHH
VADFVRQILSRLGRDSASLGTVGIVTNRGAQYGSLTTPDPVTLHETLARLADEGITDLAM
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHH
EASSHGIEQRRLDGVELTAAAFTNLGRDHLDYHPTIEDYLDAKLRLFTTLLPKGCPAIVN
HHHHCCCHHHHCCCCEEHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
ADGAYAERVIGVADAAGHAIRTTGRGGDFIRLVEAGTEGFAQSLTLSHAGKDYRVRLPLV
CCCHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEE
GGFQVENALVAAGLALATPAGQADPAGVFAALEGLTGVPGRMERIGEANGGLCLVDYAHK
CCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCEEEEECCCC
PEALEHVLSALRPFASGRLVVVFGCGGNRDAGKRPIMGAIAERLADRVIVTDDNPRNEEP
CHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCC
AAIRAAILAAAPGAEEIGDRAEAIRTGVRDLRAGDVLVVAGKGHETGQIVGPRVLPFSDH
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCEEECCCCC
DTLRAAIAELNG
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]