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The map label for this gene is murF [H]

Identifier: 218532976

GI number: 218532976

Start: 5464615

End: 5466078

Strand: Reverse

Name: murF [H]

Synonym: Mchl_5100

Alternate gene names: 218532976

Gene position: 5466078-5464615 (Counterclockwise)

Preceding gene: 218532977

Following gene: 218532975

Centisome position: 94.6

GC content: 72.2

Gene sequence:

>1464_bases
ATGAGCGACGCCCCCCTCTGGACCCCCGCCGCCCTGGAAGCGGCCACCGGCGGGCGCCTGATCGGTGCGCCGCGCGCCGT
CACCGGCGCCTCGATCGACACCCGCAGCCTGGAACCCGGCGACCTGTTCTTCGCCATCCGCGGCGAGGCGCGGGACGGGC
ACGACTTCGTGCGCGCTGCCCTGGACAAGGGCGCCGGCGCGGCGGTGGTCGCGGAAGCGCGCGCGAAGGAATTCGAGGGC
GTCGGTTCGGTGCTGGCCGTGCCGGGTGAGGGCGACGATCCGGTTCTGGACGCGATGCGTCGCCTTGGTCTGGCCGCGCG
CGCCCGCACCGAGGCAGCGATCGTCGCGGTGACCGGCTCCGTCGGCAAGACCGGTACCAAGGAAGCCCTGCGCCACGTGC
TCTCGGCGCAAGGCGCCACGCACGCCTCGGTCGCCTCCTACAACAACCATTGGGGCGTGCCGCTGACGCTGACGCGGATG
CCGGAGGCCACCCGCTTCGGCGTGTTCGAGATCGGCATGAACCACGGCGCCGAGATCCTGCCGCTGACCGCGATGGTGCG
GCCGGACGTGGCGCTGATCACCACGGTCGAGCCGGTGCATATCGAGCATTTCCGCTCGCTCTCGGCCATCGCCGACGCAA
AGGGCGAGATTTTTTCCGGGCTCAAGCCCGGCGGCGTTGCGGTGATCAACCGCGACAACCCGAACTACGAGCGCCTGCTC
GCCCACGCGCAGGCCTCGCGGGCCGGGCGCGTCATCACCTTCGGCGAGCACGCGGCGGCGGATGTGCGCGCTCACCGCAT
CCTGACCCGCCCCGACGTCTCGGTGGTCGATGCCACGGTGATGGGCGTTCCCGTGACCTACCAGCTCGGCACGCCGGGCC
GGCACGTCGCGATGAACTCGCTCGGCGTGCTCGCCTGCATCCACGCGCTCGGCGCCGACCTCGCCCGCGCCGCCCTGTCG
CTGGCGAGCCTCAAGCCGCCGGTCGGGCGCGGCGAGCGCACGGCGCTTCGGATCGGAGACGGTGAGGCCTTCCTCGTCGA
CGAGAGCTACAACGCCAACCCCGCCTCGATCCGCGCGGCGCTGGCGACGCTCGCCGGCATCGACACCGGCGTTAAGGGCC
GGCGCATCGCCGTGCTCGGCGATATGAAGGAGCTCGGCGCGGCGGGCGAAGCGCTGCATCGCGAGTTGGCGGATGCGGTC
GCCGCGAACGGCATCGACCTCGTCTTCGCCGTCGGCCCGCTGATGGCGCACCTGTTCGAGGCGCTGCCGATGACGGTGCG
GGGGACGGCTGCGCCGACCGCCGAGGGCGTCACCGACGCCGTCCTCGCGACCCTGCGGCCGGGCGATGCCGTGATGGTCA
AGGGCTCCAACTCCATGCGCATGGTCCGCATTGTCGAAGCGGTGAAAGCCCGCTACGCGGTCGCGCCGGACCCGCGCGAA
GCCTCGCTGAACGCGGTTCGCTAA

Upstream 100 bases:

>100_bases
TGGCCGGCAAAGGACATGAAACCGGCCAGATCGTCGGGCCACGCGTGCTCCCGTTCTCGGATCACGACACCCTGCGCGCC
GCGATCGCGGAGTTGAACGG

Downstream 100 bases:

>100_bases
GCTTCACTCCATCGGCCAGGCCCTCGCGCCCGGCCGTTCCATCCGGCCGCGCGCAACGCCGTGCCGGGCGTGTCGACAAA
GAACCTGCCGCGGGCGGACG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate/D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSDAPLWTPAALEAATGGRLIGAPRAVTGASIDTRSLEPGDLFFAIRGEARDGHDFVRAALDKGAGAAVVAEARAKEFEG
VGSVLAVPGEGDDPVLDAMRRLGLAARARTEAAIVAVTGSVGKTGTKEALRHVLSAQGATHASVASYNNHWGVPLTLTRM
PEATRFGVFEIGMNHGAEILPLTAMVRPDVALITTVEPVHIEHFRSLSAIADAKGEIFSGLKPGGVAVINRDNPNYERLL
AHAQASRAGRVITFGEHAAADVRAHRILTRPDVSVVDATVMGVPVTYQLGTPGRHVAMNSLGVLACIHALGADLARAALS
LASLKPPVGRGERTALRIGDGEAFLVDESYNANPASIRAALATLAGIDTGVKGRRIAVLGDMKELGAAGEALHRELADAV
AANGIDLVFAVGPLMAHLFEALPMTVRGTAAPTAEGVTDAVLATLRPGDAVMVKGSNSMRMVRIVEAVKARYAVAPDPRE
ASLNAVR

Sequences:

>Translated_487_residues
MSDAPLWTPAALEAATGGRLIGAPRAVTGASIDTRSLEPGDLFFAIRGEARDGHDFVRAALDKGAGAAVVAEARAKEFEG
VGSVLAVPGEGDDPVLDAMRRLGLAARARTEAAIVAVTGSVGKTGTKEALRHVLSAQGATHASVASYNNHWGVPLTLTRM
PEATRFGVFEIGMNHGAEILPLTAMVRPDVALITTVEPVHIEHFRSLSAIADAKGEIFSGLKPGGVAVINRDNPNYERLL
AHAQASRAGRVITFGEHAAADVRAHRILTRPDVSVVDATVMGVPVTYQLGTPGRHVAMNSLGVLACIHALGADLARAALS
LASLKPPVGRGERTALRIGDGEAFLVDESYNANPASIRAALATLAGIDTGVKGRRIAVLGDMKELGAAGEALHRELADAV
AANGIDLVFAVGPLMAHLFEALPMTVRGTAAPTAEGVTDAVLATLRPGDAVMVKGSNSMRMVRIVEAVKARYAVAPDPRE
ASLNAVR
>Mature_486_residues
SDAPLWTPAALEAATGGRLIGAPRAVTGASIDTRSLEPGDLFFAIRGEARDGHDFVRAALDKGAGAAVVAEARAKEFEGV
GSVLAVPGEGDDPVLDAMRRLGLAARARTEAAIVAVTGSVGKTGTKEALRHVLSAQGATHASVASYNNHWGVPLTLTRMP
EATRFGVFEIGMNHGAEILPLTAMVRPDVALITTVEPVHIEHFRSLSAIADAKGEIFSGLKPGGVAVINRDNPNYERLLA
HAQASRAGRVITFGEHAAADVRAHRILTRPDVSVVDATVMGVPVTYQLGTPGRHVAMNSLGVLACIHALGADLARAALSL
ASLKPPVGRGERTALRIGDGEAFLVDESYNANPASIRAALATLAGIDTGVKGRRIAVLGDMKELGAAGEALHRELADAVA
ANGIDLVFAVGPLMAHLFEALPMTVRGTAAPTAEGVTDAVLATLRPGDAVMVKGSNSMRMVRIVEAVKARYAVAPDPREA
SLNAVR

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=470, Percent_Identity=35.3191489361702, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50500; Mature: 50369

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAPLWTPAALEAATGGRLIGAPRAVTGASIDTRSLEPGDLFFAIRGEARDGHDFVRAA
CCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
LDKGAGAAVVAEARAKEFEGVGSVLAVPGEGDDPVLDAMRRLGLAARARTEAAIVAVTGS
HHCCCCEEEEEHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCHHHHCCCCEEEEEEECC
VGKTGTKEALRHVLSAQGATHASVASYNNHWGVPLTLTRMPEATRFGVFEIGMNHGAEIL
CCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCCCCHHCEEEEECCCCCCEEE
PLTAMVRPDVALITTVEPVHIEHFRSLSAIADAKGEIFSGLKPGGVAVINRDNPNYERLL
EEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCHHHHH
AHAQASRAGRVITFGEHAAADVRAHRILTRPDVSVVDATVMGVPVTYQLGTPGRHVAMNS
HHHHHHCCCCEEEECCCHHHHHHHHHEECCCCCHHEEHEEECCEEEEEECCCCCEEHHHH
LGVLACIHALGADLARAALSLASLKPPVGRGERTALRIGDGEAFLVDESYNANPASIRAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHH
LATLAGIDTGVKGRRIAVLGDMKELGAAGEALHRELADAVAANGIDLVFAVGPLMAHLFE
HHHHHHCCCCCCCCEEEEECCHHHHCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
ALPMTVRGTAAPTAEGVTDAVLATLRPGDAVMVKGSNSMRMVRIVEAVKARYAVAPDPRE
HCCCEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
ASLNAVR
CCCCCCC
>Mature Secondary Structure 
SDAPLWTPAALEAATGGRLIGAPRAVTGASIDTRSLEPGDLFFAIRGEARDGHDFVRAA
CCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
LDKGAGAAVVAEARAKEFEGVGSVLAVPGEGDDPVLDAMRRLGLAARARTEAAIVAVTGS
HHCCCCEEEEEHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCHHHHCCCCEEEEEEECC
VGKTGTKEALRHVLSAQGATHASVASYNNHWGVPLTLTRMPEATRFGVFEIGMNHGAEIL
CCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEECCCCCHHCEEEEECCCCCCEEE
PLTAMVRPDVALITTVEPVHIEHFRSLSAIADAKGEIFSGLKPGGVAVINRDNPNYERLL
EEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHCCCCCCCEEEEECCCCCHHHHH
AHAQASRAGRVITFGEHAAADVRAHRILTRPDVSVVDATVMGVPVTYQLGTPGRHVAMNS
HHHHHHCCCCEEEECCCHHHHHHHHHEECCCCCHHEEHEEECCEEEEEECCCCCEEHHHH
LGVLACIHALGADLARAALSLASLKPPVGRGERTALRIGDGEAFLVDESYNANPASIRAA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHH
LATLAGIDTGVKGRRIAVLGDMKELGAAGEALHRELADAVAANGIDLVFAVGPLMAHLFE
HHHHHHCCCCCCCCEEEEECCHHHHCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
ALPMTVRGTAAPTAEGVTDAVLATLRPGDAVMVKGSNSMRMVRIVEAVKARYAVAPDPRE
HCCCEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCC
ASLNAVR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893; 9274032 [H]