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The map label for this gene is mraY

Identifier: 218532975

GI number: 218532975

Start: 5463427

End: 5464509

Strand: Reverse

Name: mraY

Synonym: Mchl_5099

Alternate gene names: 218532975

Gene position: 5464509-5463427 (Counterclockwise)

Preceding gene: 218532976

Following gene: 218532974

Centisome position: 94.58

GC content: 66.48

Gene sequence:

>1083_bases
ATGCTCTATCTGCTGTCGGAACTGAGCGGCACGCTCACGCCCCTCAACGTGTTCCGCTACATCACCTTCCGCACCGGCGG
CGCGCTGTTCACGGCGGGCTTCTTCGTGTTCTGGTTCGGGCCCTGGATCATCTCGCTGCTGCGCCTGCGCCAGGGCAAGG
GCCAGCCGATCCGCGAGGACGGCCCGGCCAGCCACCTGACCAAGCGCGGCACGCCGACCATGGGCGGCCTGATGATCCTG
GCCGGCGCCGTGGTCTCGATCCTGCTCTGGACCAACCCGCACAACCACTACGTCTGGGTGACGCTCGCGGTGACCCTCGG
CTTCGGCGCGATCGGCTTCTACGACGACTACCTCAAGGTGACGAAGCAGTCGCACAAGGGCTTCTCGGGCCGCTTCCGCC
TGCTGCTGGAATTCGCGATCGCGGGCGCGGCCTGCCTGATGATCTCGCTCTACTCGCCCGCCGGCCTGCAGAACCAGCTC
GCTTTCCCCGTCCTCAAGGACACGCTGCTGAATCTCGGCTGGTTCTGGGTGCCGTTCGCCGCCTTCGTGATCGTGGGGGC
GGGCAACGCGGTCAACATCACCGACGGCCTCGACGGGCTCGCCATCGTCCCCGTCATGATCGCCTGCGCCACCTTCGGCA
TCATCGCCTACCTCGTCGGCAACGTCATCTATGCCGGCTACCTGCAAGTGAACTACGTGCGCGACACCGGCGAACTCGCC
GTGGTCTGCGGCGCGGTGATCGGGGCGGGGCTCGGCTTCCTGTGGTTCAACGCGCCGCCCGCGCAGATCTTCATGGGCGA
TACCGGCTCGCTCGCGCTCGGCGGCCTGCTCGGCTCCATCGCCGTGGCGGCCAAGCACGAGATCGTGCTCGCCATCGTCG
GCGGCCTGTTCGTCTTGGAGATCATGTCTGTGATCATCCAGGTCGTCTCGTTCAAGCTCACGGGCAAGCGCGTCTTCCGC
ATGGCGCCGATCCACCACCACTTCGAGCAGAAGGGCTGGAAGGAGCCGCAGGTCGTGATCCGCTTCTGGATCATCGCCGT
CATCCTGGCGCTGGTCGGGCTCGCCACGCTGAAGCTGCGTTGA

Upstream 100 bases:

>100_bases
ACTCCATCGGCCAGGCCCTCGCGCCCGGCCGTTCCATCCGGCCGCGCGCAACGCCGTGCCGGGCGTGTCGACAAAGAACC
TGCCGCGGGCGGACGAACGC

Downstream 100 bases:

>100_bases
GCCGGGACGAGAGACATAAAAACCATGACGCCAGCCACCACCTTCGCCGGCCAGAAACTCGCCCTGTTCGGCCTTGGCGG
GTCCGGGCTCGTCACTGTCC

Product: phospho-N-acetylmuramoyl-pentapeptide- transferase

Products: NA

Alternate protein names: UDP-MurNAc-pentapeptide phosphotransferase

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MLYLLSELSGTLTPLNVFRYITFRTGGALFTAGFFVFWFGPWIISLLRLRQGKGQPIREDGPASHLTKRGTPTMGGLMIL
AGAVVSILLWTNPHNHYVWVTLAVTLGFGAIGFYDDYLKVTKQSHKGFSGRFRLLLEFAIAGAACLMISLYSPAGLQNQL
AFPVLKDTLLNLGWFWVPFAAFVIVGAGNAVNITDGLDGLAIVPVMIACATFGIIAYLVGNVIYAGYLQVNYVRDTGELA
VVCGAVIGAGLGFLWFNAPPAQIFMGDTGSLALGGLLGSIAVAAKHEIVLAIVGGLFVLEIMSVIIQVVSFKLTGKRVFR
MAPIHHHFEQKGWKEPQVVIRFWIIAVILALVGLATLKLR

Sequences:

>Translated_360_residues
MLYLLSELSGTLTPLNVFRYITFRTGGALFTAGFFVFWFGPWIISLLRLRQGKGQPIREDGPASHLTKRGTPTMGGLMIL
AGAVVSILLWTNPHNHYVWVTLAVTLGFGAIGFYDDYLKVTKQSHKGFSGRFRLLLEFAIAGAACLMISLYSPAGLQNQL
AFPVLKDTLLNLGWFWVPFAAFVIVGAGNAVNITDGLDGLAIVPVMIACATFGIIAYLVGNVIYAGYLQVNYVRDTGELA
VVCGAVIGAGLGFLWFNAPPAQIFMGDTGSLALGGLLGSIAVAAKHEIVLAIVGGLFVLEIMSVIIQVVSFKLTGKRVFR
MAPIHHHFEQKGWKEPQVVIRFWIIAVILALVGLATLKLR
>Mature_360_residues
MLYLLSELSGTLTPLNVFRYITFRTGGALFTAGFFVFWFGPWIISLLRLRQGKGQPIREDGPASHLTKRGTPTMGGLMIL
AGAVVSILLWTNPHNHYVWVTLAVTLGFGAIGFYDDYLKVTKQSHKGFSGRFRLLLEFAIAGAACLMISLYSPAGLQNQL
AFPVLKDTLLNLGWFWVPFAAFVIVGAGNAVNITDGLDGLAIVPVMIACATFGIIAYLVGNVIYAGYLQVNYVRDTGELA
VVCGAVIGAGLGFLWFNAPPAQIFMGDTGSLALGGLLGSIAVAAKHEIVLAIVGGLFVLEIMSVIIQVVSFKLTGKRVFR
MAPIHHHFEQKGWKEPQVVIRFWIIAVILALVGLATLKLR

Specific function: First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family. MraY subfamily

Homologues:

Organism=Escherichia coli, GI1786275, Length=360, Percent_Identity=52.2222222222222, Blast_Score=364, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MRAY_METC4 (B7KU72)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002423791.1
- GeneID:   7118973
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_5099
- HOGENOM:   HBG708263
- OMA:   LRQGKGQ
- ProtClustDB:   PRK00108
- HAMAP:   MF_00038
- InterPro:   IPR000715
- InterPro:   IPR003524
- InterPro:   IPR018480
- PANTHER:   PTHR22926
- PANTHER:   PTHR22926:SF3
- TIGRFAMs:   TIGR00445

Pfam domain/function: PF00953 Glycos_transf_4; PF10555 MraY_sig1

EC number: =2.7.8.13

Molecular weight: Translated: 38971; Mature: 38971

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS01347 MRAY_1; PS01348 MRAY_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x91f9ed4)-; HASH(0x114802b4)-; HASH(0x11a41514)-; HASH(0x11edfbcc)-; HASH(0x119aa0e0)-; HASH(0x121b50c4)-; HASH(0x11720350)-; HASH(0x120290d8)-; HASH(0x118fcc9c)-; HASH(0x121c35c0)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLLSELSGTLTPLNVFRYITFRTGGALFTAGFFVFWFGPWIISLLRLRQGKGQPIRED
CEEEEECCCCCCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPASHLTKRGTPTMGGLMILAGAVVSILLWTNPHNHYVWVTLAVTLGFGAIGFYDDYLKV
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEHHCCCCHHHHHHHHHH
TKQSHKGFSGRFRLLLEFAIAGAACLMISLYSPAGLQNQLAFPVLKDTLLNLGWFWVPFA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
AFVIVGAGNAVNITDGLDGLAIVPVMIACATFGIIAYLVGNVIYAGYLQVNYVRDTGELA
HHHHEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHH
VVCGAVIGAGLGFLWFNAPPAQIFMGDTGSLALGGLLGSIAVAAKHEIVLAIVGGLFVLE
HHHHHHHHCCHHEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMSVIIQVVSFKLTGKRVFRMAPIHHHFEQKGWKEPQVVIRFWIIAVILALVGLATLKLR
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MLYLLSELSGTLTPLNVFRYITFRTGGALFTAGFFVFWFGPWIISLLRLRQGKGQPIRED
CEEEEECCCCCCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GPASHLTKRGTPTMGGLMILAGAVVSILLWTNPHNHYVWVTLAVTLGFGAIGFYDDYLKV
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEHHCCCCHHHHHHHHHH
TKQSHKGFSGRFRLLLEFAIAGAACLMISLYSPAGLQNQLAFPVLKDTLLNLGWFWVPFA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHH
AFVIVGAGNAVNITDGLDGLAIVPVMIACATFGIIAYLVGNVIYAGYLQVNYVRDTGELA
HHHHEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHH
VVCGAVIGAGLGFLWFNAPPAQIFMGDTGSLALGGLLGSIAVAAKHEIVLAIVGGLFVLE
HHHHHHHHCCHHEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMSVIIQVVSFKLTGKRVFRMAPIHHHFEQKGWKEPQVVIRFWIIAVILALVGLATLKLR
HHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA