Click here to switch to the map view.

The map label for this gene is murD [H]

Identifier: 218532974

GI number: 218532974

Start: 5462035

End: 5463402

Strand: Reverse

Name: murD [H]

Synonym: Mchl_5098

Alternate gene names: 218532974

Gene position: 5463402-5462035 (Counterclockwise)

Preceding gene: 218532975

Following gene: 218532972

Centisome position: 94.56

GC content: 71.71

Gene sequence:

>1368_bases
ATGACGCCAGCCACCACCTTCGCCGGCCAGAAACTCGCCCTGTTCGGCCTTGGCGGGTCCGGGCTCGTCACTGTCCGCTC
GCTGCTGGCCGGCGGCGCCGACGTGCTGGCCTGGGACGACAATGCGGGCAGCCGCGACCGCGCCCGCGAGCAGGGCATCA
CCGTCACCGATCTCAACGAGGCCGATTGGTCGGGCTTCTCGGCCCTGGTGCTGGCACCCGGCGTGCCGCTGACGCATCCC
GAGCCGCACTGGACCGTGGGACTGGCGCGGCAGGCCGGCATCCCGATCATCGGCGACATCGAGATTTTCTGCCGCGAGCG
CGCGGCCCTGGCACCCGACGCACCGTTCGTGGCCATCACCGGCACCAACGGGAAATCCACCACCACGGCGCTCACCGCCC
ACCTGCTGCGCCATGCCGGGCGCGACGTGCAGATGGGCGGCAATATCGGCACCGCGATCCTGTCGCTGGAGCCGCCCGCG
GCAACCCGCGTCCACGTGGTCGAGCTGTCCTCGTTCCAGATCGACCTGACGCCCTCGCTCAAGCCCGGCGTCGGCATCCT
GCTCAACCTGACGCCGGATCATCTCGACCGTCACGGCACCATGGAGAACTACGCCGCGATCAAGGAGCGGCTGGTGGCGG
GCGCGGACCTCGCGGTGATCGGCGTCGATGACCCCTACTGCGCAGCGATCGCCGAGCGCCGCACGGGCGAGCGCATCCGC
CTCCATATCGAGGGGCACGGCGACGCGGCGGGCGACTACGTCGGCGCCAACGGTACGGTGCGGGGGCCCGACGGCGCCGT
GATCGCGGAGGTCTCCGGCATCCAAGCGCTGCTTGGCGACCACAACCTGCAGAACGCGGCCGCCGCGGTTGCCGCCGCGA
TCCGGCTCGGCCTCAGCAGCGCGGAGATCGCGGCGGGGCTCAAGAGCTTTCCCGGCCTCGCCCACCGGATGGAGCCGCTG
CGCCGGATCGGCCCGACGCTGTTCGTCAACGATTCCAAGGCGACCAACGCCGATTCGACCGAGAAGGCGCTGCTGGCCTT
CCGCGACATCCACTGGATCGTCGGCGGCAAGGCGAAGGAGGGTGGCATCGAGCCGTTGGCGCCGCTGTTCGACCGCGTCG
CCCACGCCTACCTGATCGGCGCGTCGAGCGAGGCCTTCGCCGCGACGCTGGAAGGACGCGTCGCCTTCACCCGCTGCGGC
ACGCTGGAGGCCGCGACCGAGGCGGCGGCGCGGGCCGCCCGCGCATCCGGCACCGAGGCGCCCGTCGTGCTGCTGTCGCC
GGCCTGCGCCTCCTACGACCAGTTCCCGAACTTCGAGGTCCGTGGCGACCGCTTTCGGACCCTGGTCGCCGCGCTGCCGA
ACGCCTGA

Upstream 100 bases:

>100_bases
GCAGGTCGTGATCCGCTTCTGGATCATCGCCGTCATCCTGGCGCTGGTCGGGCTCGCCACGCTGAAGCTGCGTTGAGCCG
GGACGAGAGACATAAAAACC

Downstream 100 bases:

>100_bases
GGGCGAGCCGAAGCCCGCTCAGGCGCTCTTCCGCTCAGGCACTCTTGCCGACCGGCCGCGGGCTCGGGGCCGGTTCGGCG
GCCTTGGCCTCAGACGGCGT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTPATTFAGQKLALFGLGGSGLVTVRSLLAGGADVLAWDDNAGSRDRAREQGITVTDLNEADWSGFSALVLAPGVPLTHP
EPHWTVGLARQAGIPIIGDIEIFCRERAALAPDAPFVAITGTNGKSTTTALTAHLLRHAGRDVQMGGNIGTAILSLEPPA
ATRVHVVELSSFQIDLTPSLKPGVGILLNLTPDHLDRHGTMENYAAIKERLVAGADLAVIGVDDPYCAAIAERRTGERIR
LHIEGHGDAAGDYVGANGTVRGPDGAVIAEVSGIQALLGDHNLQNAAAAVAAAIRLGLSSAEIAAGLKSFPGLAHRMEPL
RRIGPTLFVNDSKATNADSTEKALLAFRDIHWIVGGKAKEGGIEPLAPLFDRVAHAYLIGASSEAFAATLEGRVAFTRCG
TLEAATEAAARAARASGTEAPVVLLSPACASYDQFPNFEVRGDRFRTLVAALPNA

Sequences:

>Translated_455_residues
MTPATTFAGQKLALFGLGGSGLVTVRSLLAGGADVLAWDDNAGSRDRAREQGITVTDLNEADWSGFSALVLAPGVPLTHP
EPHWTVGLARQAGIPIIGDIEIFCRERAALAPDAPFVAITGTNGKSTTTALTAHLLRHAGRDVQMGGNIGTAILSLEPPA
ATRVHVVELSSFQIDLTPSLKPGVGILLNLTPDHLDRHGTMENYAAIKERLVAGADLAVIGVDDPYCAAIAERRTGERIR
LHIEGHGDAAGDYVGANGTVRGPDGAVIAEVSGIQALLGDHNLQNAAAAVAAAIRLGLSSAEIAAGLKSFPGLAHRMEPL
RRIGPTLFVNDSKATNADSTEKALLAFRDIHWIVGGKAKEGGIEPLAPLFDRVAHAYLIGASSEAFAATLEGRVAFTRCG
TLEAATEAAARAARASGTEAPVVLLSPACASYDQFPNFEVRGDRFRTLVAALPNA
>Mature_454_residues
TPATTFAGQKLALFGLGGSGLVTVRSLLAGGADVLAWDDNAGSRDRAREQGITVTDLNEADWSGFSALVLAPGVPLTHPE
PHWTVGLARQAGIPIIGDIEIFCRERAALAPDAPFVAITGTNGKSTTTALTAHLLRHAGRDVQMGGNIGTAILSLEPPAA
TRVHVVELSSFQIDLTPSLKPGVGILLNLTPDHLDRHGTMENYAAIKERLVAGADLAVIGVDDPYCAAIAERRTGERIRL
HIEGHGDAAGDYVGANGTVRGPDGAVIAEVSGIQALLGDHNLQNAAAAVAAAIRLGLSSAEIAAGLKSFPGLAHRMEPLR
RIGPTLFVNDSKATNADSTEKALLAFRDIHWIVGGKAKEGGIEPLAPLFDRVAHAYLIGASSEAFAATLEGRVAFTRCGT
LEAATEAAARAARASGTEAPVVLLSPACASYDQFPNFEVRGDRFRTLVAALPNA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=455, Percent_Identity=36.2637362637363, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47299; Mature: 47168

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPATTFAGQKLALFGLGGSGLVTVRSLLAGGADVLAWDDNAGSRDRAREQGITVTDLNE
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCEEEECCC
ADWSGFSALVLAPGVPLTHPEPHWTVGLARQAGIPIIGDIEIFCRERAALAPDAPFVAIT
CCCCCCEEEEEECCCCCCCCCCCEEEEEHHHCCCCEEECHHHEECCCCCCCCCCCEEEEE
GTNGKSTTTALTAHLLRHAGRDVQMGGNIGTAILSLEPPAATRVHVVELSSFQIDLTPSL
CCCCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCEEEEEEEEEEEEEECCCC
KPGVGILLNLTPDHLDRHGTMENYAAIKERLVAGADLAVIGVDDPYCAAIAERRTGERIR
CCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEE
LHIEGHGDAAGDYVGANGTVRGPDGAVIAEVSGIQALLGDHNLQNAAAAVAAAIRLGLSS
EEEECCCCCCCCEECCCCEEECCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
AEIAAGLKSFPGLAHRMEPLRRIGPTLFVNDSKATNADSTEKALLAFRDIHWIVGGKAKE
HHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEECCCCCC
GGIEPLAPLFDRVAHAYLIGASSEAFAATLEGRVAFTRCGTLEAATEAAARAARASGTEA
CCCCHHHHHHHHHHHEEEECCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCC
PVVLLSPACASYDQFPNFEVRGDRFRTLVAALPNA
CEEEECCCCCCCCCCCCEEECCHHHHHHHHHCCCC
>Mature Secondary Structure 
TPATTFAGQKLALFGLGGSGLVTVRSLLAGGADVLAWDDNAGSRDRAREQGITVTDLNE
CCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHCCCEEEECCC
ADWSGFSALVLAPGVPLTHPEPHWTVGLARQAGIPIIGDIEIFCRERAALAPDAPFVAIT
CCCCCCEEEEEECCCCCCCCCCCEEEEEHHHCCCCEEECHHHEECCCCCCCCCCCEEEEE
GTNGKSTTTALTAHLLRHAGRDVQMGGNIGTAILSLEPPAATRVHVVELSSFQIDLTPSL
CCCCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCCCEEEEEEEEEEEEEECCCC
KPGVGILLNLTPDHLDRHGTMENYAAIKERLVAGADLAVIGVDDPYCAAIAERRTGERIR
CCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCEEE
LHIEGHGDAAGDYVGANGTVRGPDGAVIAEVSGIQALLGDHNLQNAAAAVAAAIRLGLSS
EEEECCCCCCCCEECCCCEEECCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCH
AEIAAGLKSFPGLAHRMEPLRRIGPTLFVNDSKATNADSTEKALLAFRDIHWIVGGKAKE
HHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEECCCCCC
GGIEPLAPLFDRVAHAYLIGASSEAFAATLEGRVAFTRCGTLEAATEAAARAARASGTEA
CCCCHHHHHHHHHHHEEEECCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHHCCCCCC
PVVLLSPACASYDQFPNFEVRGDRFRTLVAALPNA
CEEEECCCCCCCCCCCCEEECCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA