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The map label for this gene is phaD [H]

Identifier: 218532955

GI number: 218532955

Start: 5435710

End: 5437365

Strand: Reverse

Name: phaD [H]

Synonym: Mchl_5079

Alternate gene names: 218532955

Gene position: 5437365-5435710 (Counterclockwise)

Preceding gene: 218532956

Following gene: 218532954

Centisome position: 94.11

GC content: 70.71

Gene sequence:

>1656_bases
ATGAACGCGCCCCTCCTGAGCGGCCCCGGCGCGGTCCTGTGGTCCGATCATCTCGTCGTCCTGCCGATCGTGCTGCCGAT
CGCCGCGGCCGGTGTGATGTTTCTGATGGACGAGCGGCGCCACCGCCTGAAGTCGGCGATCAGTCTCGCCACCGTGCTGG
TGCTGCTCGGCATCGCCCTCGCCCTCGTCGGCCGGGCGGCCGAGGCGCCTGAGGTCTACCGCCTCGGCAACTGGGCCGCG
CCTTACGGCATCGTGCTGGTGGCCGACCGGCTCTCGGCGGTGATGCTGACCCTGGCCGGCGTGCTGGGACTTGCCGCCCT
CGTCTTCTCCTTCGCCCGCTGGGGCCGGGCCGGCCCGCGCTTCCACGGCCTGTTCCTGCTGCTGCTGATGGGGGTGAACG
GCGCCTTCCTCACCGGTGACGTGTTCAACCTGTTCGTGTTCTTCGAGGTGATGCTGGCGGCCTCTTACGGCCTCGTCCTG
CATGGCTCGGGCGAGACCCGCATCCGGGCAGGCCTCGGCTACATCGCCGTCAACCTCGTCGCTTCGCTGTTCTTCCTCAT
CGGTGTCAGCCTGATCTACGGCACGATGGGCACGCTCAACATGGCCGACCTCGCCCGGCGGCTCGCGAGCCCGCCCGCTG
AGGCCGCCCTGTTCGAGGTCGGCTGCGCCGTGCTCGGCATCGCCTTCCTCATCAAGTGCAGCGCCTGGCCCCTCGGCTTC
TGGCTGCCGACCACCTATTCCGCCGCCACCGCCCCCGCGGCGGCGATCCTGGCGATCCTCAGCAAAGTCGGCGTCTACGT
CGTGATCCGCCTCAGCCTGCTCCTGTTCGGGGACGGCAGCTCGGCAGGGTTCGGCCAGAGCTGGATCCTCGTCACCGGCC
TCGCGACCATCGCCTTCGGCACCTTCGGCATCCTGGCCGCGCGCCATCTGACGCGGGCAGCGGGCTACGCGGTGATGATC
TCGTCGGGCACGGTGCTCGCGGCGCTCGGCACCGGCAGCGCGGCGGCGCTCTCCGGCGCGCTCTACTACCTCGTCGGCTC
GACCTACGCGGCCGGCGCCCTGTTCCTGCTGGCCGAGATCCTGCAGCGTGCCCGCGACCCGGCGGATGCGGCCCAACCCG
TCTTCGCCGACGAGTACCGCGATCCCTTCGACGATGCCGACGCCGTCGAGGTCGGCCGGGCGATCCCCGCGGCGGTGGCG
ATCCTCAGCAGCGGCTTCCTGCTCTGCACGCTGATGCTCGCCGGAATCCCGCCGCTCTCGGGCTTCGTCGGCAAGCTGGC
GATGTTCTCGGGGCTGGCCTCGAACACCATGACCGCCTCGGCCTGGTGGCTGATCGCCGCGCTCACCCTCTCCAGCCTCG
GCACCCTGATTCCCCTTGTGCGGCTCGGCATCTTCAGCCTGTGGGTGCCCCATGACGACCCGCCGCCGACGATGCGGACG
CCGGAACTCGCAAGCCTCAGCGTCCTGCTCGCGGCCTGCATCGCGCTCGCGCTGTGGGGCGGGCCGGCGCTCGCCTACAG
CGAGGCCACCGTGCGCTGGCTGTCGCAGCCGCAGGACTACGTCCGCGCCGTCCTCGATTCGGGCAAAGGTGAAGCGGGCA
AGAGCAGCGCAGGCAAAAGCGGCGCGGGCGAGGGTGCAGTGGGGGCAAGGCCATGA

Upstream 100 bases:

>100_bases
TCCTCGTGGTGCTGCTCGCAGCCCGCGGCATCACCGGCAGCGATCACGTCGACGGCCGGGAGCCGCATCACGCCGAACCC
ACGCCGGAGAACCCGCATCC

Downstream 100 bases:

>100_bases
ACGCGCTTCTGCCCCACCCCTTCCTCTCCGCCGGCCTCGTCGTGATGTGGCTGCTGCTGAACGCGCCGCCGACGCCCGGC
AACGTTCTGCTCGCGCTCCT

Product: monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MNAPLLSGPGAVLWSDHLVVLPIVLPIAAAGVMFLMDERRHRLKSAISLATVLVLLGIALALVGRAAEAPEVYRLGNWAA
PYGIVLVADRLSAVMLTLAGVLGLAALVFSFARWGRAGPRFHGLFLLLLMGVNGAFLTGDVFNLFVFFEVMLAASYGLVL
HGSGETRIRAGLGYIAVNLVASLFFLIGVSLIYGTMGTLNMADLARRLASPPAEAALFEVGCAVLGIAFLIKCSAWPLGF
WLPTTYSAATAPAAAILAILSKVGVYVVIRLSLLLFGDGSSAGFGQSWILVTGLATIAFGTFGILAARHLTRAAGYAVMI
SSGTVLAALGTGSAAALSGALYYLVGSTYAAGALFLLAEILQRARDPADAAQPVFADEYRDPFDDADAVEVGRAIPAAVA
ILSSGFLLCTLMLAGIPPLSGFVGKLAMFSGLASNTMTASAWWLIAALTLSSLGTLIPLVRLGIFSLWVPHDDPPPTMRT
PELASLSVLLAACIALALWGGPALAYSEATVRWLSQPQDYVRAVLDSGKGEAGKSSAGKSGAGEGAVGARP

Sequences:

>Translated_551_residues
MNAPLLSGPGAVLWSDHLVVLPIVLPIAAAGVMFLMDERRHRLKSAISLATVLVLLGIALALVGRAAEAPEVYRLGNWAA
PYGIVLVADRLSAVMLTLAGVLGLAALVFSFARWGRAGPRFHGLFLLLLMGVNGAFLTGDVFNLFVFFEVMLAASYGLVL
HGSGETRIRAGLGYIAVNLVASLFFLIGVSLIYGTMGTLNMADLARRLASPPAEAALFEVGCAVLGIAFLIKCSAWPLGF
WLPTTYSAATAPAAAILAILSKVGVYVVIRLSLLLFGDGSSAGFGQSWILVTGLATIAFGTFGILAARHLTRAAGYAVMI
SSGTVLAALGTGSAAALSGALYYLVGSTYAAGALFLLAEILQRARDPADAAQPVFADEYRDPFDDADAVEVGRAIPAAVA
ILSSGFLLCTLMLAGIPPLSGFVGKLAMFSGLASNTMTASAWWLIAALTLSSLGTLIPLVRLGIFSLWVPHDDPPPTMRT
PELASLSVLLAACIALALWGGPALAYSEATVRWLSQPQDYVRAVLDSGKGEAGKSSAGKSGAGEGAVGARP
>Mature_551_residues
MNAPLLSGPGAVLWSDHLVVLPIVLPIAAAGVMFLMDERRHRLKSAISLATVLVLLGIALALVGRAAEAPEVYRLGNWAA
PYGIVLVADRLSAVMLTLAGVLGLAALVFSFARWGRAGPRFHGLFLLLLMGVNGAFLTGDVFNLFVFFEVMLAASYGLVL
HGSGETRIRAGLGYIAVNLVASLFFLIGVSLIYGTMGTLNMADLARRLASPPAEAALFEVGCAVLGIAFLIKCSAWPLGF
WLPTTYSAATAPAAAILAILSKVGVYVVIRLSLLLFGDGSSAGFGQSWILVTGLATIAFGTFGILAARHLTRAAGYAVMI
SSGTVLAALGTGSAAALSGALYYLVGSTYAAGALFLLAEILQRARDPADAAQPVFADEYRDPFDDADAVEVGRAIPAAVA
ILSSGFLLCTLMLAGIPPLSGFVGKLAMFSGLASNTMTASAWWLIAALTLSSLGTLIPLVRLGIFSLWVPHDDPPPTMRT
PELASLSVLLAACIALALWGGPALAYSEATVRWLSQPQDYVRAVLDSGKGEAGKSSAGKSGAGEGAVGARP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=310, Percent_Identity=32.9032258064516, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693160, Length=483, Percent_Identity=26.2939958592132, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788831, Length=339, Percent_Identity=25.6637168141593, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788829, Length=391, Percent_Identity=23.5294117647059, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 57008; Mature: 57008

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPLLSGPGAVLWSDHLVVLPIVLPIAAAGVMFLMDERRHRLKSAISLATVLVLLGIAL
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVGRAAEAPEVYRLGNWAAPYGIVLVADRLSAVMLTLAGVLGLAALVFSFARWGRAGPR
HHHHCCCCCCCHHCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
FHGLFLLLLMGVNGAFLTGDVFNLFVFFEVMLAASYGLVLHGSGETRIRAGLGYIAVNLV
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHH
ASLFFLIGVSLIYGTMGTLNMADLARRLASPPAEAALFEVGCAVLGIAFLIKCSAWPLGF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WLPTTYSAATAPAAAILAILSKVGVYVVIRLSLLLFGDGSSAGFGQSWILVTGLATIAFG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
TFGILAARHLTRAAGYAVMISSGTVLAALGTGSAAALSGALYYLVGSTYAAGALFLLAEI
HHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
LQRARDPADAAQPVFADEYRDPFDDADAVEVGRAIPAAVAILSSGFLLCTLMLAGIPPLS
HHHCCCCCHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHH
GFVGKLAMFSGLASNTMTASAWWLIAALTLSSLGTLIPLVRLGIFSLWVPHDDPPPTMRT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCC
PELASLSVLLAACIALALWGGPALAYSEATVRWLSQPQDYVRAVLDSGKGEAGKSSAGKS
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC
GAGEGAVGARP
CCCCCCCCCCC
>Mature Secondary Structure
MNAPLLSGPGAVLWSDHLVVLPIVLPIAAAGVMFLMDERRHRLKSAISLATVLVLLGIAL
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVGRAAEAPEVYRLGNWAAPYGIVLVADRLSAVMLTLAGVLGLAALVFSFARWGRAGPR
HHHHCCCCCCCHHCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
FHGLFLLLLMGVNGAFLTGDVFNLFVFFEVMLAASYGLVLHGSGETRIRAGLGYIAVNLV
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHH
ASLFFLIGVSLIYGTMGTLNMADLARRLASPPAEAALFEVGCAVLGIAFLIKCSAWPLGF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
WLPTTYSAATAPAAAILAILSKVGVYVVIRLSLLLFGDGSSAGFGQSWILVTGLATIAFG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
TFGILAARHLTRAAGYAVMISSGTVLAALGTGSAAALSGALYYLVGSTYAAGALFLLAEI
HHHHHHHHHHHHHCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
LQRARDPADAAQPVFADEYRDPFDDADAVEVGRAIPAAVAILSSGFLLCTLMLAGIPPLS
HHHCCCCCHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHH
GFVGKLAMFSGLASNTMTASAWWLIAALTLSSLGTLIPLVRLGIFSLWVPHDDPPPTMRT
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCC
PELASLSVLLAACIALALWGGPALAYSEATVRWLSQPQDYVRAVLDSGKGEAGKSSAGKS
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCC
GAGEGAVGARP
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]