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The map label for this gene is phaE [H]

Identifier: 218532954

GI number: 218532954

Start: 5435225

End: 5435713

Strand: Reverse

Name: phaE [H]

Synonym: Mchl_5078

Alternate gene names: 218532954

Gene position: 5435713-5435225 (Counterclockwise)

Preceding gene: 218532955

Following gene: 218532953

Centisome position: 94.08

GC content: 69.53

Gene sequence:

>489_bases
ATGAACGCGCTTCTGCCCCACCCCTTCCTCTCCGCCGGCCTCGTCGTGATGTGGCTGCTGCTGAACGCGCCGCCGACGCC
CGGCAACGTTCTGCTCGCGCTCCTCGTCGGGCTGACCGTGCCGGCGGTGATGCGGGCGCTGCGCCCGAGGCCGGTGCGGG
TCCGCCGCCCCGGTCTCGCCGTGCGGCTGCTGTTCACCGTCCTCTACGACATGCTGCGCTCCAACATCGACGTGGCCAAG
ATCATCCTCGGCCTGCATCGCGGCGAGCGGCGCACCGGCTTCGTCTCGGTCCCCCTCGACATGACGACGCCGTTCGGCCT
GTCGGTGCTGTCGATCATCCTGACCGCCACGCCCGGCACCCTCTGGGTCCAGTACGATTCCCATACCGGGCGCCTGCTGA
TGCACCTTCTCGACGACACCGACAGCGAGGGCTGGGTCCGCCGCGTCAAGGACCGCTACGAGCGCCCCCTGATGGAGATC
TTCGCGTGA

Upstream 100 bases:

>100_bases
AGGACTACGTCCGCGCCGTCCTCGATTCGGGCAAAGGTGAAGCGGGCAAGAGCAGCGCAGGCAAAAGCGGCGCGGGCGAG
GGTGCAGTGGGGGCAAGGCC

Downstream 100 bases:

>100_bases
GCGCCGCCACCGTCCTCGATTGGGGCCTCGGCCTCGCCCAAGGCATGCTGGTGCTGGCGATGGCGCTGGCCGCGTGGCGG
ATGCTGCGCGGCCCCCGCGC

Product: cation antiporter

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein E; Pha system subunit E [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MNALLPHPFLSAGLVVMWLLLNAPPTPGNVLLALLVGLTVPAVMRALRPRPVRVRRPGLAVRLLFTVLYDMLRSNIDVAK
IILGLHRGERRTGFVSVPLDMTTPFGLSVLSIILTATPGTLWVQYDSHTGRLLMHLLDDTDSEGWVRRVKDRYERPLMEI
FA

Sequences:

>Translated_162_residues
MNALLPHPFLSAGLVVMWLLLNAPPTPGNVLLALLVGLTVPAVMRALRPRPVRVRRPGLAVRLLFTVLYDMLRSNIDVAK
IILGLHRGERRTGFVSVPLDMTTPFGLSVLSIILTATPGTLWVQYDSHTGRLLMHLLDDTDSEGWVRRVKDRYERPLMEI
FA
>Mature_162_residues
MNALLPHPFLSAGLVVMWLLLNAPPTPGNVLLALLVGLTVPAVMRALRPRPVRVRRPGLAVRLLFTVLYDMLRSNIDVAK
IILGLHRGERRTGFVSVPLDMTTPFGLSVLSIILTATPGTLWVQYDSHTGRLLMHLLDDTDSEGWVRRVKDRYERPLMEI
FA

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG1863

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhE subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004847
- InterPro:   IPR002758 [H]

Pfam domain/function: PF01899 MNHE [H]

EC number: NA

Molecular weight: Translated: 18128; Mature: 18128

Theoretical pI: Translated: 11.21; Mature: 11.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALLPHPFLSAGLVVMWLLLNAPPTPGNVLLALLVGLTVPAVMRALRPRPVRVRRPGLA
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHH
VRLLFTVLYDMLRSNIDVAKIILGLHRGERRTGFVSVPLDMTTPFGLSVLSIILTATPGT
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCE
LWVQYDSHTGRLLMHLLDDTDSEGWVRRVKDRYERPLMEIFA
EEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNALLPHPFLSAGLVVMWLLLNAPPTPGNVLLALLVGLTVPAVMRALRPRPVRVRRPGLA
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHH
VRLLFTVLYDMLRSNIDVAKIILGLHRGERRTGFVSVPLDMTTPFGLSVLSIILTATPGT
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCE
LWVQYDSHTGRLLMHLLDDTDSEGWVRRVKDRYERPLMEIFA
EEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]