Click here to switch to the map view.

The map label for this gene is pncB [H]

Identifier: 218532899

GI number: 218532899

Start: 5368925

End: 5370223

Strand: Reverse

Name: pncB [H]

Synonym: Mchl_5022

Alternate gene names: 218532899

Gene position: 5370223-5368925 (Counterclockwise)

Preceding gene: 218532900

Following gene: 218532898

Centisome position: 92.94

GC content: 67.44

Gene sequence:

>1299_bases
ATGATCGACCTCGCGAAGCGGGTCTACGACCACAACTTCCGCATCGACCCGATGGTGCGCTCGCTGCTCGATACGGATTT
CTACAAGCTCCTGATGGCGCAGATGATCTTCCGCCGTCACCGCGACCTGCGCGTCAGCTTCGGAATCCAGAACCGCACCC
GGAGCGTGCGGGTCGCCGACGAGATCGACCTCGGGGAATTGCGGGCGCAGCTCGACCACGCCCGCACGATCCGCCTGAGC
CGAGGTGAATCCACTTGGCTTCGCGGCAACACTTTCTACGGCAAGCGTCAGATCCTCTCCTCCGAGTTCATGGCCTGGCT
CGAAGCGTTTCGCCTGCCGGAATACGAGTTGGAGGTGCGCGACGGACAGGTGGCGCTGACCTTCCATGGCCCCTGGGTCG
AGACCACGATGTGGGAGGTGCCGGCTCTCGCAATCCTCAACGAGATGCGCTCCCGCGCCGTGCTGCGGGGCCTCGGCAAG
TTCGAGCTGCAGGTGCTCTACGCCCGCGCCATGACCCGCGTCTGGGAGAAGATCGAGCGCCTGAAGCGCCTGCGCGACCT
CTCCATCGCCGATTTCGGCACGCGCCGCCGCCACGGCTTCCTGTGGCAGGATTGGTGCGTGCAGGCGATGAGCGAGGGCC
TGGGCGAACGCGCCTTCCTCGGCACCTCGAACTGCCTGATCGCCATGCGCCGCGAGGTCGAGGCGGTGGGCACCAACGCC
CACGAACTGCCGATGGTCTACGCCGCGCTCGCCGGAGACGACGATGAGGCGTTGGCGGCCGCGCCCTACGCGGTGCTGGC
CGACTGGCAGCAGGACTATGCCGGCAACCTGCTGGTGGCCTTGCCCGACACCTACGGCACTACCGGCTTCCTCGCCAAGG
CGCCGGACTGGCTCACGGCCTGGACCGGCATCCGCATCGATTCGAAGGAGCCGATCGCGGGCGGCGAGGAGGCGCTCGCC
TTCTGGGAGTCGCGAGGCTGCGATCCGCGCGAAAAGCTGGCGATCTTCTCCGACGGGCTCGATATCGACGACATCGAGGC
GATCCACGCCCATTTCCACGGCCGCATGCGCATCGGCTACGGCTGGGGCACGCGGCTGACCAACGATTTTCGCGGCCTCG
TGCCGGAGGGGCGGCTCGATCCGATCTCGGTCGTCTGCAAAGTGACGGAGGCCGAGGGCCGCCCGACGGTGAAGATCTCC
GACAATCCGAGCAAGGCGCAGGGGCCGGCGGCGGAGGTGGAGCGCTACAAGCGGGTGTTCGGGGTCGAGGAGCAGGCCGC
GATGGCGGTGGCGGTGTGA

Upstream 100 bases:

>100_bases
GGGGGCGCCTTTTTTGTCATGACGCCCCGCGCCCGCCGGCGTTGATCGCCGGGGCGATCCGCCCCAAGCTCGGGCGACAC
TCCCCGAACACGGACGGCCC

Downstream 100 bases:

>100_bases
GAGCAGAAGCATGAGCGCCGCCCGCATCGATCTCAACGCCGATCTCGGCGAGGGTTTCGGGCCCTGGCGGATGGGCAACG
ACGCCGCGCTCCTCGATATC

Product: nicotinate phosphoribosyltransferase

Products: NA

Alternate protein names: NAPRTase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIDLAKRVYDHNFRIDPMVRSLLDTDFYKLLMAQMIFRRHRDLRVSFGIQNRTRSVRVADEIDLGELRAQLDHARTIRLS
RGESTWLRGNTFYGKRQILSSEFMAWLEAFRLPEYELEVRDGQVALTFHGPWVETTMWEVPALAILNEMRSRAVLRGLGK
FELQVLYARAMTRVWEKIERLKRLRDLSIADFGTRRRHGFLWQDWCVQAMSEGLGERAFLGTSNCLIAMRREVEAVGTNA
HELPMVYAALAGDDDEALAAAPYAVLADWQQDYAGNLLVALPDTYGTTGFLAKAPDWLTAWTGIRIDSKEPIAGGEEALA
FWESRGCDPREKLAIFSDGLDIDDIEAIHAHFHGRMRIGYGWGTRLTNDFRGLVPEGRLDPISVVCKVTEAEGRPTVKIS
DNPSKAQGPAAEVERYKRVFGVEEQAAMAVAV

Sequences:

>Translated_432_residues
MIDLAKRVYDHNFRIDPMVRSLLDTDFYKLLMAQMIFRRHRDLRVSFGIQNRTRSVRVADEIDLGELRAQLDHARTIRLS
RGESTWLRGNTFYGKRQILSSEFMAWLEAFRLPEYELEVRDGQVALTFHGPWVETTMWEVPALAILNEMRSRAVLRGLGK
FELQVLYARAMTRVWEKIERLKRLRDLSIADFGTRRRHGFLWQDWCVQAMSEGLGERAFLGTSNCLIAMRREVEAVGTNA
HELPMVYAALAGDDDEALAAAPYAVLADWQQDYAGNLLVALPDTYGTTGFLAKAPDWLTAWTGIRIDSKEPIAGGEEALA
FWESRGCDPREKLAIFSDGLDIDDIEAIHAHFHGRMRIGYGWGTRLTNDFRGLVPEGRLDPISVVCKVTEAEGRPTVKIS
DNPSKAQGPAAEVERYKRVFGVEEQAAMAVAV
>Mature_432_residues
MIDLAKRVYDHNFRIDPMVRSLLDTDFYKLLMAQMIFRRHRDLRVSFGIQNRTRSVRVADEIDLGELRAQLDHARTIRLS
RGESTWLRGNTFYGKRQILSSEFMAWLEAFRLPEYELEVRDGQVALTFHGPWVETTMWEVPALAILNEMRSRAVLRGLGK
FELQVLYARAMTRVWEKIERLKRLRDLSIADFGTRRRHGFLWQDWCVQAMSEGLGERAFLGTSNCLIAMRREVEAVGTNA
HELPMVYAALAGDDDEALAAAPYAVLADWQQDYAGNLLVALPDTYGTTGFLAKAPDWLTAWTGIRIDSKEPIAGGEEALA
FWESRGCDPREKLAIFSDGLDIDDIEAIHAHFHGRMRIGYGWGTRLTNDFRGLVPEGRLDPISVVCKVTEAEGRPTVKIS
DNPSKAQGPAAEVERYKRVFGVEEQAAMAVAV

Specific function: NAD biosynthesis; nicotinamide to NaMN; second step. [C]

COG id: COG1488

COG function: function code H; Nicotinic acid phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAPRTase family [H]

Homologues:

Organism=Escherichia coli, GI1787162, Length=401, Percent_Identity=31.9201995012469, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324783, Length=429, Percent_Identity=28.4382284382284, Blast_Score=156, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006406
- InterPro:   IPR015977
- InterPro:   IPR007229
- InterPro:   IPR002638 [H]

Pfam domain/function: PF04095 NAPRTase [H]

EC number: =2.4.2.11 [H]

Molecular weight: Translated: 49010; Mature: 49010

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLAKRVYDHNFRIDPMVRSLLDTDFYKLLMAQMIFRRHRDLRVSFGIQNRTRSVRVAD
CCCHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEC
EIDLGELRAQLDHARTIRLSRGESTWLRGNTFYGKRQILSSEFMAWLEAFRLPEYELEVR
CCCHHHHHHHHHHHHEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
DGQVALTFHGPWVETTMWEVPALAILNEMRSRAVLRGLGKFELQVLYARAMTRVWEKIER
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LKRLRDLSIADFGTRRRHGFLWQDWCVQAMSEGLGERAFLGTSNCLIAMRREVEAVGTNA
HHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCC
HELPMVYAALAGDDDEALAAAPYAVLADWQQDYAGNLLVALPDTYGTTGFLAKAPDWLTA
HHHHEEEEEECCCCCCHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCHHHH
WTGIRIDSKEPIAGGEEALAFWESRGCDPREKLAIFSDGLDIDDIEAIHAHFHGRMRIGY
EECEEECCCCCCCCCHHHHHHHHHCCCCHHHHEEEEECCCCCHHHHHHHHHHCCEEEEEE
GWGTRLTNDFRGLVPEGRLDPISVVCKVTEAEGRPTVKISDNPSKAQGPAAEVERYKRVF
CCCCCCCCHHHCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
GVEEQAAMAVAV
CCCHHHHEEECC
>Mature Secondary Structure
MIDLAKRVYDHNFRIDPMVRSLLDTDFYKLLMAQMIFRRHRDLRVSFGIQNRTRSVRVAD
CCCHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEC
EIDLGELRAQLDHARTIRLSRGESTWLRGNTFYGKRQILSSEFMAWLEAFRLPEYELEVR
CCCHHHHHHHHHHHHEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
DGQVALTFHGPWVETTMWEVPALAILNEMRSRAVLRGLGKFELQVLYARAMTRVWEKIER
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LKRLRDLSIADFGTRRRHGFLWQDWCVQAMSEGLGERAFLGTSNCLIAMRREVEAVGTNA
HHHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCC
HELPMVYAALAGDDDEALAAAPYAVLADWQQDYAGNLLVALPDTYGTTGFLAKAPDWLTA
HHHHEEEEEECCCCCCHHHHCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCHHHH
WTGIRIDSKEPIAGGEEALAFWESRGCDPREKLAIFSDGLDIDDIEAIHAHFHGRMRIGY
EECEEECCCCCCCCCHHHHHHHHHCCCCHHHHEEEEECCCCCHHHHHHHHHHCCEEEEEE
GWGTRLTNDFRGLVPEGRLDPISVVCKVTEAEGRPTVKISDNPSKAQGPAAEVERYKRVF
CCCCCCCCHHHCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
GVEEQAAMAVAV
CCCHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA