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The map label for this gene is prfC [H]

Identifier: 218532729

GI number: 218532729

Start: 5160573

End: 5162192

Strand: Reverse

Name: prfC [H]

Synonym: Mchl_4851

Alternate gene names: 218532729

Gene position: 5162192-5160573 (Counterclockwise)

Preceding gene: 218532730

Following gene: 218532723

Centisome position: 89.34

GC content: 66.79

Gene sequence:

>1620_bases
ATGCTGATGCAAACAGACGCCAAATCCGCTGACGCGAAGACCGATCCGGTTGCGCGGCGGCGCACCTTCGCCATCATCTC
GCACCCGGATGCGGGCAAAACCACGCTCACGGAAAAGCTGCTGCTGTTCGGCGGCGCGATCCAGCTCGCGGGCGAGGTCA
AGGCCAAGCGCAACCGCGTCTCCACCCGGTCCGACTGGATGGGCATCGAGAAGGAGCGCGGCATCTCGGTCGTCACCTCG
GTGATGACCTTCGAATACGGCGACTGCGTCTTCAACCTGCTCGACACGCCGGGCCACGAGGACTTCTCGGAGGACACCTA
CCGCACCCTGACGGCCGTCGACTCGGCCGTGATGGTCATCGACGCCGCCAAGGGCATCGAGGCCAGAACGCGAAAGCTGT
TCGAGGTGTGCCGGCTGCGGGACATCCCGATCGTCACCTTCGTGAACAAGCTCGACCGAGAGGCGCGCGACCCGTTCGAG
ACGCTCGACGAAATCGAGAAGACGCTCGCCCTCGACGTGGCGCCCGTGACTTGGCCGATCGGGCTCGGGCGCAACTTCGC
CGGCACCTACGAACTCGGCGGCAACCGCGTGCGCCGGCTCGACGCGGCGGACGATGCCGGCACGATCCCGGTCTCGGGCT
TTGGCGACCCGCTCTTCGACACGCTGCTGACGGAGAACGGCGCGGCCGATGCGTGGCGCGAGGAGGTGGAGCTCGCCGAG
GGCGGTCTCAAGACCTTCGATCTCGAAGCTTTTCGCGAGGGACACCTGACCCCGGTTTTCTTCGGTTCGGCCTTGCGCAA
TTTCGGCGTGCGCGACCTCATCGACGGGCTCGCCCGCTTCGCGCCGCCCCCGCGCGGCCAGGAGGCGGACAAGCGCGCCG
TGCAGCCGTCCGAGCCGAAGATGACCGGCTTCGTGTTCAAGATCCAGGCGAACATGGATCCGAACCACCGCGACCGCATC
GCCTTCATGCGGGTCTGTTCGGGCCAGCTCCGGCGCGGGATGAAGGCCAAGCTGGTGCGCACGGGCAAGCCGATGTCGCT
CTCGGCGCCGCAATTCTTCTTCGCCCAGGACCGCGCCATCGCCGACGAGGCCTATGCCGGAGATGTGGTCGGCATTCCCA
ACCACGGGACGCTTCGCATCGGCGACACGCTGACCGAGGGCGAGGAGATCGTCTTCCGCGGGGTGCCGAGCTTCGCCCCC
GAAATCATGCGGCGGATCAAGCTCACCGACGCGATGAAGGCCAAGAAGCTCCGGGAAGCGCTCCAGCAGATGGGCGAGGA
GGGCGTCGTGCAGGTCTTCATCCCGCAGGACGGCTCGCCCGCCATCGTCGGCGTGGTCGGCGCGCTGCAGCTCGACGTGC
TGAAGGAGCGGCTCCAGGCGGAATACGGCCTGCCGATCGACTACGAGACGACGCGATTCTCGGTCTGCCGCTGGATCGAG
GCGGAATCGGAGGTCGAGATCGATCGGTTCGTGAACGCCCACGGCTCGGCCATGGCGAGCGACCTCGACGGCGCGCCGGT
CTTCATGGCCACCACCGCCTTCTCACTCCGCTACGAGGAGGAGCGCTGGGAGAAGATCCGGTTCACGGACGTGAAGGATT
ATCAGAAGCGGGCGGCGTAA

Upstream 100 bases:

>100_bases
CGGCTTTGCGTAGGCGGCGCGGAACGCGTAGAGAGCGGAACACCGCCCGGTCCCCCTTCGAGACGAGCGGCCGACCGCCC
CGCGGCGGCCGGTGCCCGCC

Downstream 100 bases:

>100_bases
GCGGCGTCTCCCGACCCCGCCATCCCACTCTTTCCCCTATCCCGAGGTGCCGCGGAGCGGCCTCGAAGGAGGGGTCCGGA
CGTCGCGCGATCCCTGGAGC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLMQTDAKSADAKTDPVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRVSTRSDWMGIEKERGISVVTS
VMTFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIVTFVNKLDREARDPFE
TLDEIEKTLALDVAPVTWPIGLGRNFAGTYELGGNRVRRLDAADDAGTIPVSGFGDPLFDTLLTENGAADAWREEVELAE
GGLKTFDLEAFREGHLTPVFFGSALRNFGVRDLIDGLARFAPPPRGQEADKRAVQPSEPKMTGFVFKIQANMDPNHRDRI
AFMRVCSGQLRRGMKAKLVRTGKPMSLSAPQFFFAQDRAIADEAYAGDVVGIPNHGTLRIGDTLTEGEEIVFRGVPSFAP
EIMRRIKLTDAMKAKKLREALQQMGEEGVVQVFIPQDGSPAIVGVVGALQLDVLKERLQAEYGLPIDYETTRFSVCRWIE
AESEVEIDRFVNAHGSAMASDLDGAPVFMATTAFSLRYEEERWEKIRFTDVKDYQKRAA

Sequences:

>Translated_539_residues
MLMQTDAKSADAKTDPVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRVSTRSDWMGIEKERGISVVTS
VMTFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIVTFVNKLDREARDPFE
TLDEIEKTLALDVAPVTWPIGLGRNFAGTYELGGNRVRRLDAADDAGTIPVSGFGDPLFDTLLTENGAADAWREEVELAE
GGLKTFDLEAFREGHLTPVFFGSALRNFGVRDLIDGLARFAPPPRGQEADKRAVQPSEPKMTGFVFKIQANMDPNHRDRI
AFMRVCSGQLRRGMKAKLVRTGKPMSLSAPQFFFAQDRAIADEAYAGDVVGIPNHGTLRIGDTLTEGEEIVFRGVPSFAP
EIMRRIKLTDAMKAKKLREALQQMGEEGVVQVFIPQDGSPAIVGVVGALQLDVLKERLQAEYGLPIDYETTRFSVCRWIE
AESEVEIDRFVNAHGSAMASDLDGAPVFMATTAFSLRYEEERWEKIRFTDVKDYQKRAA
>Mature_539_residues
MLMQTDAKSADAKTDPVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRVSTRSDWMGIEKERGISVVTS
VMTFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIVTFVNKLDREARDPFE
TLDEIEKTLALDVAPVTWPIGLGRNFAGTYELGGNRVRRLDAADDAGTIPVSGFGDPLFDTLLTENGAADAWREEVELAE
GGLKTFDLEAFREGHLTPVFFGSALRNFGVRDLIDGLARFAPPPRGQEADKRAVQPSEPKMTGFVFKIQANMDPNHRDRI
AFMRVCSGQLRRGMKAKLVRTGKPMSLSAPQFFFAQDRAIADEAYAGDVVGIPNHGTLRIGDTLTEGEEIVFRGVPSFAP
EIMRRIKLTDAMKAKKLREALQQMGEEGVVQVFIPQDGSPAIVGVVGALQLDVLKERLQAEYGLPIDYETTRFSVCRWIE
AESEVEIDRFVNAHGSAMASDLDGAPVFMATTAFSLRYEEERWEKIRFTDVKDYQKRAA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=482, Percent_Identity=27.3858921161826, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI19923640, Length=518, Percent_Identity=25.6756756756757, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI25306283, Length=412, Percent_Identity=27.4271844660194, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI25306287, Length=490, Percent_Identity=24.8979591836735, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI157426893, Length=159, Percent_Identity=35.2201257861635, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI94966754, Length=142, Percent_Identity=31.6901408450704, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4503483, Length=159, Percent_Identity=31.4465408805031, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=52.2988505747126, Blast_Score=547, Evalue=1e-157,
Organism=Escherichia coli, GI1789738, Length=495, Percent_Identity=27.4747474747475, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788922, Length=157, Percent_Identity=34.3949044585987, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=33.5570469798658, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=494, Percent_Identity=27.3279352226721, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=169, Percent_Identity=33.7278106508876, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=32.8467153284672, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988811, Length=151, Percent_Identity=29.1390728476821, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=151, Percent_Identity=29.1390728476821, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17506493, Length=189, Percent_Identity=26.4550264550265, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=28.1837160751566, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6322359, Length=537, Percent_Identity=23.0912476722533, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=177, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=180, Percent_Identity=30, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320593, Length=180, Percent_Identity=30, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24582462, Length=490, Percent_Identity=25.9183673469388, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI221458488, Length=521, Percent_Identity=24.9520153550864, Blast_Score=138, Evalue=1e-32,
Organism=Drosophila melanogaster, GI78706572, Length=175, Percent_Identity=33.7142857142857, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585709, Length=181, Percent_Identity=28.1767955801105, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585711, Length=181, Percent_Identity=28.1767955801105, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585713, Length=181, Percent_Identity=28.1767955801105, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28574573, Length=144, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59816; Mature: 59816

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMQTDAKSADAKTDPVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRV
CCCCCCCCCCCCCCCCHHHCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHC
STRSDWMGIEKERGISVVTSVMTFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVI
CCHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEE
DAAKGIEARTRKLFEVCRLRDIPIVTFVNKLDREARDPFETLDEIEKTLALDVAPVTWPI
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEC
GLGRNFAGTYELGGNRVRRLDAADDAGTIPVSGFGDPLFDTLLTENGAADAWREEVELAE
CCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHH
GGLKTFDLEAFREGHLTPVFFGSALRNFGVRDLIDGLARFAPPPRGQEADKRAVQPSEPK
CCCCEEEHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCC
MTGFVFKIQANMDPNHRDRIAFMRVCSGQLRRGMKAKLVRTGKPMSLSAPQFFFAQDRAI
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEECCCHHH
ADEAYAGDVVGIPNHGTLRIGDTLTEGEEIVFRGVPSFAPEIMRRIKLTDAMKAKKLREA
HHHHCCCCEEECCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LQQMGEEGVVQVFIPQDGSPAIVGVVGALQLDVLKERLQAEYGLPIDYETTRFSVCRWIE
HHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
AESEVEIDRFVNAHGSAMASDLDGAPVFMATTAFSLRYEEERWEKIRFTDVKDYQKRAA
CCCCCCHHHHHCCCCCHHHCCCCCCCEEEEEEHHHEEECHHHHHEEECCCHHHHHHHCC
>Mature Secondary Structure
MLMQTDAKSADAKTDPVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGEVKAKRNRV
CCCCCCCCCCCCCCCCHHHCEEEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHC
STRSDWMGIEKERGISVVTSVMTFEYGDCVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVI
CCHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEEEE
DAAKGIEARTRKLFEVCRLRDIPIVTFVNKLDREARDPFETLDEIEKTLALDVAPVTWPI
ECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEC
GLGRNFAGTYELGGNRVRRLDAADDAGTIPVSGFGDPLFDTLLTENGAADAWREEVELAE
CCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHH
GGLKTFDLEAFREGHLTPVFFGSALRNFGVRDLIDGLARFAPPPRGQEADKRAVQPSEPK
CCCCEEEHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCC
MTGFVFKIQANMDPNHRDRIAFMRVCSGQLRRGMKAKLVRTGKPMSLSAPQFFFAQDRAI
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEECCCHHH
ADEAYAGDVVGIPNHGTLRIGDTLTEGEEIVFRGVPSFAPEIMRRIKLTDAMKAKKLREA
HHHHCCCCEEECCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
LQQMGEEGVVQVFIPQDGSPAIVGVVGALQLDVLKERLQAEYGLPIDYETTRFSVCRWIE
HHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
AESEVEIDRFVNAHGSAMASDLDGAPVFMATTAFSLRYEEERWEKIRFTDVKDYQKRAA
CCCCCCHHHHHCCCCCHHHCCCCCCCEEEEEEHHHEEECHHHHHEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA