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The map label for this gene is alkR [H]

Identifier: 218532719

GI number: 218532719

Start: 5151649

End: 5152554

Strand: Reverse

Name: alkR [H]

Synonym: Mchl_4841

Alternate gene names: 218532719

Gene position: 5152554-5151649 (Counterclockwise)

Preceding gene: 218532721

Following gene: 218532718

Centisome position: 89.18

GC content: 67.0

Gene sequence:

>906_bases
ATGGCATCCATGACCGGATCACTGGAAAACTTGACCGTTCTTCCGGACGCCGGACCGGGCGTCTCCGGCGACCTGCTCTC
GCACGTACTGGCGCAGATCCGGCTCACTGGGGACCATGTCCGGTCACACGAAGTCGCGTGGTCGAACGTCTTGGAGCTGG
AAGCCGATACCGCCCATGTCCTGGTGGTCGCCGACGGCTCGCTCGACGTCGAGGACGATGGCGGGGAGCGCGTCGTGATC
GACACGGGCGATCTCGTGCTTCTGCCCCGCGGGCTCGGAGGGTCTAGGCTGGTGGCGTCCGCCTCGCACGCATCGGTGTT
CGTCTGCCGGTTCTCGTTCGATCCCCACAGCCTTCGCGGCATGATCTCGGCCCTGCCCGGGCGCATCCACATCCGGCGCG
CCGAAGGAGCCGGGTGGCTTGACGGCATCGTACCCTTCCTGATGGTCGAGGTGACTGACGACGAGCCCGGCGCGGCCCTG
ATGATCTCACGGATCATCGACGTCATCGTCATCCGCACCTTGCGGACCTGGGTGCAGCGCGGGCACACCACCGGATGGCT
GGGAGGGTTGTCCGACGCCCGCATCGCCCGAGCGTTGAAGGTCATACACGAGCGACCACTGCAGCGCTGGAGCGTCGACA
CCCTAGCCGACGCGGCCGGCATGTCCCGGTCGAGCTTCTGCGAACGTTTCGCCGCGCTCGTCGGGCGCTCGCCACTGCTC
TACCAGAACGAGTGGCGGCTCACGCTCGCCCGGAACATGCTCGTCGCCCGCGAGGCACGTGTCGGCGAGGTCGCACTACG
GGTCGGCTATGAGTCCGAAGCATCGTTCAGCCGTGCTTACAAGGCGATGTTCGGCCATCCACCGAGGGACGAGCCGGCAC
TGCCAAGCGGACGTCTCGGCCGGTAG

Upstream 100 bases:

>100_bases
CGCGGCAGCTTCGGCGACCGAAACGCCAGGCTTCGTGAAAGCGGCGTCTTTTCAGAAAGATACGCGTCGCTTCGGAAGGT
ACGTTTACAATCGGACGCCG

Downstream 100 bases:

>100_bases
GCTTGAAGGTCTGCAAGGGGTCGGGAGTTCGCACGCCGATGGTTCTGCTGTGCGCCACACCCGGACCTCGGCCTTCGGTC
CAGAAGCGGACCTTCGGCTT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MASMTGSLENLTVLPDAGPGVSGDLLSHVLAQIRLTGDHVRSHEVAWSNVLELEADTAHVLVVADGSLDVEDDGGERVVI
DTGDLVLLPRGLGGSRLVASASHASVFVCRFSFDPHSLRGMISALPGRIHIRRAEGAGWLDGIVPFLMVEVTDDEPGAAL
MISRIIDVIVIRTLRTWVQRGHTTGWLGGLSDARIARALKVIHERPLQRWSVDTLADAAGMSRSSFCERFAALVGRSPLL
YQNEWRLTLARNMLVAREARVGEVALRVGYESEASFSRAYKAMFGHPPRDEPALPSGRLGR

Sequences:

>Translated_301_residues
MASMTGSLENLTVLPDAGPGVSGDLLSHVLAQIRLTGDHVRSHEVAWSNVLELEADTAHVLVVADGSLDVEDDGGERVVI
DTGDLVLLPRGLGGSRLVASASHASVFVCRFSFDPHSLRGMISALPGRIHIRRAEGAGWLDGIVPFLMVEVTDDEPGAAL
MISRIIDVIVIRTLRTWVQRGHTTGWLGGLSDARIARALKVIHERPLQRWSVDTLADAAGMSRSSFCERFAALVGRSPLL
YQNEWRLTLARNMLVAREARVGEVALRVGYESEASFSRAYKAMFGHPPRDEPALPSGRLGR
>Mature_300_residues
ASMTGSLENLTVLPDAGPGVSGDLLSHVLAQIRLTGDHVRSHEVAWSNVLELEADTAHVLVVADGSLDVEDDGGERVVID
TGDLVLLPRGLGGSRLVASASHASVFVCRFSFDPHSLRGMISALPGRIHIRRAEGAGWLDGIVPFLMVEVTDDEPGAALM
ISRIIDVIVIRTLRTWVQRGHTTGWLGGLSDARIARALKVIHERPLQRWSVDTLADAAGMSRSSFCERFAALVGRSPLLY
QNEWRLTLARNMLVAREARVGEVALRVGYESEASFSRAYKAMFGHPPRDEPALPSGRLGR

Specific function: This protein activates the expression of the alkane 1- monooxygenase AlkM [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786496, Length=230, Percent_Identity=29.1304347826087, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082197, Length=136, Percent_Identity=35.2941176470588, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32653; Mature: 32522

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASMTGSLENLTVLPDAGPGVSGDLLSHVLAQIRLTGDHVRSHEVAWSNVLELEADTAHV
CCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCHHHHHHEEEEECCCEEE
LVVADGSLDVEDDGGERVVIDTGDLVLLPRGLGGSRLVASASHASVFVCRFSFDPHSLRG
EEEECCCEEECCCCCCEEEEECCCEEEEECCCCCCCEEEECCCCEEEEEEECCCHHHHHH
MISALPGRIHIRRAEGAGWLDGIVPFLMVEVTDDEPGAALMISRIIDVIVIRTLRTWVQR
HHHHCCCEEEEEECCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GHTTGWLGGLSDARIARALKVIHERPLQRWSVDTLADAAGMSRSSFCERFAALVGRSPLL
CCCCEECCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCE
YQNEWRLTLARNMLVAREARVGEVALRVGYESEASFSRAYKAMFGHPPRDEPALPSGRLG
ECCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
R
C
>Mature Secondary Structure 
ASMTGSLENLTVLPDAGPGVSGDLLSHVLAQIRLTGDHVRSHEVAWSNVLELEADTAHV
CCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCHHHHHHEEEEECCCEEE
LVVADGSLDVEDDGGERVVIDTGDLVLLPRGLGGSRLVASASHASVFVCRFSFDPHSLRG
EEEECCCEEECCCCCCEEEEECCCEEEEECCCCCCCEEEECCCCEEEEEEECCCHHHHHH
MISALPGRIHIRRAEGAGWLDGIVPFLMVEVTDDEPGAALMISRIIDVIVIRTLRTWVQR
HHHHCCCEEEEEECCCCCCHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GHTTGWLGGLSDARIARALKVIHERPLQRWSVDTLADAAGMSRSSFCERFAALVGRSPLL
CCCCEECCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCE
YQNEWRLTLARNMLVAREARVGEVALRVGYESEASFSRAYKAMFGHPPRDEPALPSGRLG
ECCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]