Click here to switch to the map view.

The map label for this gene is trxB [H]

Identifier: 218532718

GI number: 218532718

Start: 5149736

End: 5151352

Strand: Reverse

Name: trxB [H]

Synonym: Mchl_4840

Alternate gene names: 218532718

Gene position: 5151352-5149736 (Counterclockwise)

Preceding gene: 218532719

Following gene: 218532717

Centisome position: 89.16

GC content: 66.48

Gene sequence:

>1617_bases
ATGGAAACGATCGGCCGTGATCTACGCGAGATGCAGCGCATCCCCTTGGCCGAATCGCATGTCGCCGCGCTTCGGGCGAT
CGGAGCCGAGAAGCACTACCCGGCCGGTACCTTTCTCGTCCATGCCGGTGATCCAGCCGACCGCTTCGTCTATGTCGAGG
CCGGCGAGATCGAGGTGGTCAACCCTTTCACAAATGAGCGCCATCTGCCGTCTACCCTCGGACCGACTCAGTTCATGGGC
GAGATCTCGTTCCTGAACGGCGGGGTCTGGTCGATGCCCATGCGGGCGAGTGCGGATACCATCGTCACCGAAGTGCCACG
CCCGGAGATGCTGCGACTGATGTCCGAGGTCCCGGAGATGTCGGACATCATCATCACGGTCCTGGCCGCACGGCGGCGGC
GGCAGCTCGACTCGCACGACGGCACGCTGGTGCTCCTCGGCGAGGACGACGACCGGAGCGTCCGGCGGATTGCCGAGTTC
GCGAGCCGGAACCGCCTTCCCTACAGGTCCTATCCGCTGGGCAGCCCGGAGGCGGCGAGCGTCGCCAGGAGTTGCTCGAT
CTCGTCCGAACGGCCGGCTGTGATCTTCGGCCGGGATATCGTCGTATCCGAGCCGACACCCGCCAAGGTCGCGCGGCTGC
TCGGCCTCGACTACAACCTCGTCGACGACGAGGCCTTCGACGTCCTTATCGTGGGCGGCGGCCCGGCCGGGGTGGCAGCG
GGCGTCTATGCGGGCGCGGAGGGGCTTCGCGCGCTGGTCATCGAGGATGTGGCGATCGGCGGTCAGGCCGGCACATCGAG
CCGGATCGAGAACTATATGGGCTTTCCGACCGGGATCTCGGGCGCCGACCTCGTCTGGCGCGGCGAGGTCCAGGCGATGA
AGTTCGGCACGCGGTTCGCGATGCCGCGGCGCGTGGCGAAGCTGGAACGGCTAGAGGACGGATCGTTCTGTGCAACGTTC
GACAACGGCCGGCGCGTTCGGGGACAGGCCGTCGTTGTCGCGACCGGCGTCCAGTACCGAAGATTGCCGATCGAACGCCT
TGAGGCGTTCGAGGGTGCAGGCATCTACTACGCTGCGACGGGGATCGAGGCACGCTACTGCAGGAATGCCGAGACGATCA
TCATCGGCGGCGGCAACTCAGCCGGCCAGGCAGCGATGTATCTCAGCCGCTCGGCCCGACACGTTCGCCTTCTCGTGCGC
GGGCCATCGCTTGCGACGTCCATGTCGAACTACCTGTCGAGCCGGCTGGAGGCCGACCCGGGGATCACGATCGAGTACGA
GGCCGAAATCACCGCCCTTCACGGCGACGATCACCTCGATGCGGTGACGATCCGCAACGTCGCCGACGGCACGATGCGCA
CGATCTCGACCTGTGCCGTTTTCGTCATGGTGGGCGCCGCCCCCAACACCGGCTGGCTCTCGGGGCTCGTCGAACTCGAT
GGCCACGGCTTCGTCCTAACCGGGGACGCGATCGGCGCGCGTTCACCCTATGCGACGTCGCATCCGGGAATCTTCGCGGT
CGGCGACGTGCGAGCCAACTCCGTGAAGCGTGTCGCAGCCTCGGTCGGCGAGGGTTCGGTCGTCATCTCCAAGGTGTGGG
AGCACGTTCGGACGTAG

Upstream 100 bases:

>100_bases
CTGCAGCTTCGACCATGCATACGCTCGCGCGCAGGACTTGCCTACCGAAGCTCAGCCACTGATGCTGCGCCGAAGCGGAT
GACCTGCGAGGGGGCGACCC

Downstream 100 bases:

>100_bases
ATCACGCCTCCCCTGCCGCGTCCGACCGGGGCGCTGGATCTATCGACGACCGGGCCCGCGCTCCTGCCGTTTCAGCCGGC
GCTGCGACGGCTTGGGCGAT

Product: cyclic nucleotide-regulated FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: TRXR [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
METIGRDLREMQRIPLAESHVAALRAIGAEKHYPAGTFLVHAGDPADRFVYVEAGEIEVVNPFTNERHLPSTLGPTQFMG
EISFLNGGVWSMPMRASADTIVTEVPRPEMLRLMSEVPEMSDIIITVLAARRRRQLDSHDGTLVLLGEDDDRSVRRIAEF
ASRNRLPYRSYPLGSPEAASVARSCSISSERPAVIFGRDIVVSEPTPAKVARLLGLDYNLVDDEAFDVLIVGGGPAGVAA
GVYAGAEGLRALVIEDVAIGGQAGTSSRIENYMGFPTGISGADLVWRGEVQAMKFGTRFAMPRRVAKLERLEDGSFCATF
DNGRRVRGQAVVVATGVQYRRLPIERLEAFEGAGIYYAATGIEARYCRNAETIIIGGGNSAGQAAMYLSRSARHVRLLVR
GPSLATSMSNYLSSRLEADPGITIEYEAEITALHGDDHLDAVTIRNVADGTMRTISTCAVFVMVGAAPNTGWLSGLVELD
GHGFVLTGDAIGARSPYATSHPGIFAVGDVRANSVKRVAASVGEGSVVISKVWEHVRT

Sequences:

>Translated_538_residues
METIGRDLREMQRIPLAESHVAALRAIGAEKHYPAGTFLVHAGDPADRFVYVEAGEIEVVNPFTNERHLPSTLGPTQFMG
EISFLNGGVWSMPMRASADTIVTEVPRPEMLRLMSEVPEMSDIIITVLAARRRRQLDSHDGTLVLLGEDDDRSVRRIAEF
ASRNRLPYRSYPLGSPEAASVARSCSISSERPAVIFGRDIVVSEPTPAKVARLLGLDYNLVDDEAFDVLIVGGGPAGVAA
GVYAGAEGLRALVIEDVAIGGQAGTSSRIENYMGFPTGISGADLVWRGEVQAMKFGTRFAMPRRVAKLERLEDGSFCATF
DNGRRVRGQAVVVATGVQYRRLPIERLEAFEGAGIYYAATGIEARYCRNAETIIIGGGNSAGQAAMYLSRSARHVRLLVR
GPSLATSMSNYLSSRLEADPGITIEYEAEITALHGDDHLDAVTIRNVADGTMRTISTCAVFVMVGAAPNTGWLSGLVELD
GHGFVLTGDAIGARSPYATSHPGIFAVGDVRANSVKRVAASVGEGSVVISKVWEHVRT
>Mature_538_residues
METIGRDLREMQRIPLAESHVAALRAIGAEKHYPAGTFLVHAGDPADRFVYVEAGEIEVVNPFTNERHLPSTLGPTQFMG
EISFLNGGVWSMPMRASADTIVTEVPRPEMLRLMSEVPEMSDIIITVLAARRRRQLDSHDGTLVLLGEDDDRSVRRIAEF
ASRNRLPYRSYPLGSPEAASVARSCSISSERPAVIFGRDIVVSEPTPAKVARLLGLDYNLVDDEAFDVLIVGGGPAGVAA
GVYAGAEGLRALVIEDVAIGGQAGTSSRIENYMGFPTGISGADLVWRGEVQAMKFGTRFAMPRRVAKLERLEDGSFCATF
DNGRRVRGQAVVVATGVQYRRLPIERLEAFEGAGIYYAATGIEARYCRNAETIIIGGGNSAGQAAMYLSRSARHVRLLVR
GPSLATSMSNYLSSRLEADPGITIEYEAEITALHGDDHLDAVTIRNVADGTMRTISTCAVFVMVGAAPNTGWLSGLVELD
GHGFVLTGDAIGARSPYATSHPGIFAVGDVRANSVKRVAASVGEGSVVISKVWEHVRT

Specific function: Component of the thioredoxin-thioredoxin reductase system which may be involved in biosynthesis of penicillins and cephalosporins and may be important in determining the thiol- disulfide redox balance [H]

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=304, Percent_Identity=30.9210526315789, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081763, Length=306, Percent_Identity=28.4313725490196, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320560, Length=312, Percent_Identity=29.8076923076923, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321898, Length=312, Percent_Identity=28.8461538461538, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR005982 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.8.1.9 [H]

Molecular weight: Translated: 57804; Mature: 57804

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00888 CNMP_BINDING_1 ; PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIGRDLREMQRIPLAESHVAALRAIGAEKHYPAGTFLVHAGDPADRFVYVEAGEIEVV
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCCEEEE
NPFTNERHLPSTLGPTQFMGEISFLNGGVWSMPMRASADTIVTEVPRPEMLRLMSEVPEM
CCCCCCCCCCCCCCCHHHHCEEEEECCCEEECCCCCCCCHHEECCCCHHHHHHHHHCCCH
SDIIITVLAARRRRQLDSHDGTLVLLGEDDDRSVRRIAEFASRNRLPYRSYPLGSPEAAS
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VARSCSISSERPAVIFGRDIVVSEPTPAKVARLLGLDYNLVDDEAFDVLIVGGGPAGVAA
HHHHCCCCCCCCEEEEECEEEECCCCHHHHHHHHCCCCEEECCCCEEEEEECCCCHHHHH
GVYAGAEGLRALVIEDVAIGGQAGTSSRIENYMGFPTGISGADLVWRGEVQAMKFGTRFA
HHHHCCCCCEEEEEEHEEECCCCCCHHHHHHHCCCCCCCCCCCEEEECCEEEEECCHHHC
MPRRVAKLERLEDGSFCATFDNGRRVRGQAVVVATGVQYRRLPIERLEAFEGAGIYYAAT
CHHHHHHHHHCCCCCEEEEECCCCEECCCEEEEEECCCEECCCHHHHHHHCCCCEEEEEC
GIEARYCRNAETIIIGGGNSAGQAAMYLSRSARHVRLLVRGPSLATSMSNYLSSRLEADP
CCCHHHCCCCCEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCC
GITIEYEAEITALHGDDHLDAVTIRNVADGTMRTISTCAVFVMVGAAPNTGWLSGLVELD
CEEEEEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHEEEEEEECCCCCCHHHEEEEEEC
GHGFVLTGDAIGARSPYATSHPGIFAVGDVRANSVKRVAASVGEGSVVISKVWEHVRT
CCEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
METIGRDLREMQRIPLAESHVAALRAIGAEKHYPAGTFLVHAGDPADRFVYVEAGEIEVV
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCCEEEE
NPFTNERHLPSTLGPTQFMGEISFLNGGVWSMPMRASADTIVTEVPRPEMLRLMSEVPEM
CCCCCCCCCCCCCCCHHHHCEEEEECCCEEECCCCCCCCHHEECCCCHHHHHHHHHCCCH
SDIIITVLAARRRRQLDSHDGTLVLLGEDDDRSVRRIAEFASRNRLPYRSYPLGSPEAAS
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VARSCSISSERPAVIFGRDIVVSEPTPAKVARLLGLDYNLVDDEAFDVLIVGGGPAGVAA
HHHHCCCCCCCCEEEEECEEEECCCCHHHHHHHHCCCCEEECCCCEEEEEECCCCHHHHH
GVYAGAEGLRALVIEDVAIGGQAGTSSRIENYMGFPTGISGADLVWRGEVQAMKFGTRFA
HHHHCCCCCEEEEEEHEEECCCCCCHHHHHHHCCCCCCCCCCCEEEECCEEEEECCHHHC
MPRRVAKLERLEDGSFCATFDNGRRVRGQAVVVATGVQYRRLPIERLEAFEGAGIYYAAT
CHHHHHHHHHCCCCCEEEEECCCCEECCCEEEEEECCCEECCCHHHHHHHCCCCEEEEEC
GIEARYCRNAETIIIGGGNSAGQAAMYLSRSARHVRLLVRGPSLATSMSNYLSSRLEADP
CCCHHHCCCCCEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCC
GITIEYEAEITALHGDDHLDAVTIRNVADGTMRTISTCAVFVMVGAAPNTGWLSGLVELD
CEEEEEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHEEEEEEECCCCCCHHHEEEEEEC
GHGFVLTGDAIGARSPYATSHPGIFAVGDVRANSVKRVAASVGEGSVVISKVWEHVRT
CCEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8349555; 8423136 [H]