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The map label for this gene is ribE [H]

Identifier: 218710375

GI number: 218710375

Start: 2581464

End: 2582120

Strand: Reverse

Name: ribE [H]

Synonym: VS_2412

Alternate gene names: 218710375

Gene position: 2582120-2581464 (Counterclockwise)

Preceding gene: 218710376

Following gene: 218710374

Centisome position: 78.26

GC content: 45.05

Gene sequence:

>657_bases
ATGTTTACAGGAATTGTAGAAGCCGTAGGTACATTGAGTGCAATCACTCCCCGCGGAGAAGACATCACCGTAACGGTTAA
TGTTGGTAATCTTGATATGGCTGACGTTAAGTTAGGCGACAGTATCGCGACCAATGGTGTTTGTTTGACGGTTGTTGAGT
ATAACGATCACAGCTACAGTGCAGACCTTTCGCTTGAGACCCTGAAAAAAACGGGTTTTGTCGATTACCAAGCTGGCGAC
AAGGTAAACCTAGAAAAAGCAATGTTACCGACCACGCGTTTCGGTGGTCATATCGTATCGGGTCACGTTGATGGCGTGGG
TGAGATTGTTGAGCGTAACCAAGTCGGTCGTGCGATTGAGTTCTGGGTTGAAATGCCAGCAGAGATCTCAAAGTACGTAG
CTCAAAAAGGTTCAGTCACCGTTGATGGTATTAGCTTGACGGTGAATGATTTACGCAAGAATGCCTTTAAGCTGACTATC
GTTCCTCATACCTCTTCAGAAACGACCATAGATCAATTCAATGTTGGTCGCAAAGTGAATCTAGAAGTCGATGTATTAGC
GCGCTATATGGAGCGTTTACTGCAAGGCCAGCAGCAAGAGTCTGAGCCTGAATCTCGATTAACAATGGAATTCTTACAGC
AGAATGGTTTTGCTTAA

Upstream 100 bases:

>100_bases
GTCGCGAAACCTATTATCGCGAAACAAATAATAACGAAACCATAGTGGAAATAGTTCTGCTAACCAAATACATGTCGTTG
ACAACAAAAAGAGTTTTAAA

Downstream 100 bases:

>100_bases
CCGTTTGTACAAAGCTTGCTAAAGAGTACTTTGTAAAAGCGCAGCAATATCATCAGGTTTAAATAGTGTCGGTTCTAGGA
AGCAGAACCATTTCAAAGGA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MFTGIVEAVGTLSAITPRGEDITVTVNVGNLDMADVKLGDSIATNGVCLTVVEYNDHSYSADLSLETLKKTGFVDYQAGD
KVNLEKAMLPTTRFGGHIVSGHVDGVGEIVERNQVGRAIEFWVEMPAEISKYVAQKGSVTVDGISLTVNDLRKNAFKLTI
VPHTSSETTIDQFNVGRKVNLEVDVLARYMERLLQGQQQESEPESRLTMEFLQQNGFA

Sequences:

>Translated_218_residues
MFTGIVEAVGTLSAITPRGEDITVTVNVGNLDMADVKLGDSIATNGVCLTVVEYNDHSYSADLSLETLKKTGFVDYQAGD
KVNLEKAMLPTTRFGGHIVSGHVDGVGEIVERNQVGRAIEFWVEMPAEISKYVAQKGSVTVDGISLTVNDLRKNAFKLTI
VPHTSSETTIDQFNVGRKVNLEVDVLARYMERLLQGQQQESEPESRLTMEFLQQNGFA
>Mature_218_residues
MFTGIVEAVGTLSAITPRGEDITVTVNVGNLDMADVKLGDSIATNGVCLTVVEYNDHSYSADLSLETLKKTGFVDYQAGD
KVNLEKAMLPTTRFGGHIVSGHVDGVGEIVERNQVGRAIEFWVEMPAEISKYVAQKGSVTVDGISLTVNDLRKNAFKLTI
VPHTSSETTIDQFNVGRKVNLEVDVLARYMERLLQGQQQESEPESRLTMEFLQQNGFA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=204, Percent_Identity=34.3137254901961, Blast_Score=114, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319733, Length=221, Percent_Identity=38.0090497737557, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23856; Mature: 23856

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEAVGTLSAITPRGEDITVTVNVGNLDMADVKLGDSIATNGVCLTVVEYNDHSYS
CCCHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCEEEEEEEECCCCEE
ADLSLETLKKTGFVDYQAGDKVNLEKAMLPTTRFGGHIVSGHVDGVGEIVERNQVGRAIE
CCCCHHHHHHCCCEEECCCCCCCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCHHHH
FWVEMPAEISKYVAQKGSVTVDGISLTVNDLRKNAFKLTIVPHTSSETTIDQFNVGRKVN
HHHHCCHHHHHHHHCCCCEEEEEEEEEHHHHCCCEEEEEEEECCCCCCCHHHHCCCCEEE
LEVDVLARYMERLLQGQQQESEPESRLTMEFLQQNGFA
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFTGIVEAVGTLSAITPRGEDITVTVNVGNLDMADVKLGDSIATNGVCLTVVEYNDHSYS
CCCHHHHHHHHHHHCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCEEEEEEEECCCCEE
ADLSLETLKKTGFVDYQAGDKVNLEKAMLPTTRFGGHIVSGHVDGVGEIVERNQVGRAIE
CCCCHHHHHHCCCEEECCCCCCCHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCHHHH
FWVEMPAEISKYVAQKGSVTVDGISLTVNDLRKNAFKLTIVPHTSSETTIDQFNVGRKVN
HHHHCCHHHHHHHHCCCCEEEEEEEEEHHHHCCCEEEEEEEECCCCCCCHHHHCCCCEEE
LEVDVLARYMERLLQGQQQESEPESRLTMEFLQQNGFA
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]