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The map label for this gene is thiI

Identifier: 218710364

GI number: 218710364

Start: 2570457

End: 2571905

Strand: Reverse

Name: thiI

Synonym: VS_2401

Alternate gene names: 218710364

Gene position: 2571905-2570457 (Counterclockwise)

Preceding gene: 218710365

Following gene: 218710363

Centisome position: 77.95

GC content: 44.03

Gene sequence:

>1449_bases
ATGAAATTTATTGTTAAGCCCCACCCGGAAATTTTTGTAAAAAGTGAATCGGTGCGTAAGCGCTTCACAAAGATTCTAGA
ACGTAATATTCGTACTATTCTTCAGCGTCGTACTGAGTCTGTGGCTGTCTTCAACCGCCGTGACCATATCGAAGTGACGT
CTGAGAGCGACAAATACTTTAAAGAAACACTTGAAGTATTGACGCAAACTCCGGGTATTCATCACTCCCTTGAAGTTCAG
CAATCAGAATTTAAAGACCTGCACAATATTTACGAGCAAGTTCTCGAGCGTAGCGGTGCTCTTATTGAAGGTAAGACTTT
CTCTGTACGTGCTAAGCGTCGTGGTAAGCATGATTTTACTTCTATCGAGCTAGAACGCTACGTAGGCGGCGGTCTAAATC
AAGCGGTTGAATCAGCAAAAGTAAAACTGAAAAATCCAGATATTACCGTTAAAGTTGAAGTCGAGAACGATAAGCTAAAC
CAAGTTATTGAACGTCATAAAGGCCTAGGTGGTTTCCCTCTTGGTACTCAAGAAGATCTGCTTAGCTTGATCTCTGGTGG
CTTTGATTCTGGTGTTTCAAGCTACTTACACATCAAACGTGGTTCTAAAGTGCATTACTGCTTCTTCAATCTTGGTGGCC
CTGCTCACGAGATTGGTGTTAAGCAGGTTTCTCATTATCTATGGGATAAATACGGTTCATCTGCAAAGGTGAAGTTCATC
TCTATTGATTTTGAACCTGTAGTTGCAGAGATCCTTGAGAACGTTGAAGACGGTCAAATGGGCGTTGTTCTTAAGCGTAT
GTTCATGCGTGCTGGTGGTATGGTTGCAGAGCGTTTTGGTATCGAAGCACTAGTAACGGGTGAAGCTCTTGGTCAGGTTT
CTAGCCAAACGCTAACTAACCTGCGTCATATCGACAACGTGACGGACACTTTGATCCTTCGTCCACTTATCAACTGGGAC
AAAGAAGACATCATCGACCTGTCTCGTAAGATTGGTACTGAAGACTTCGCTAAAGTAATGCCTGAGTATTGTGGTGTTAT
CTCTAAGAAGCCAACCGTGAAAGCGAAGAAAGGCAAACTAGAAGCGGAAGAAGCTAAGTTTAACTTCGAAGTGCTAGAGC
AAGTGATCGAGAATGCTCGTATTATGGATATCCGTGATATCGAGAAAGAGAGCCAAGAACAGGCACCAGAAGTGGAGCAA
GTTCAAGCCGTTGCTGAGCACGCTGTCGTTCTTGATATCCGTAGCCCAGACGAAGAAGATGAAAGCCCGCTAGAGATCGA
TGGTGTTGAAATCAAACACATCCCGTTCTTCAAGCTTTCAACTCAGTTTGGTGACCTAGACCAATCGAAAGAGTACTTGT
TGTACTGTGACCGTGGTGTCATGAGCCGTCTGCAAGCGCTTTACTTGCAAGAACAAGGCTTCCACAACGTTAAGGTTTAC
CGCCCATAG

Upstream 100 bases:

>100_bases
TTTAATATTGATCTATTATTGCTTCAATAACTATGTGAAATGACCTTTTACGTGGTGATGAACCTATTAAAATTGTGAAC
CTTATTGGCGTGAAAGTACT

Downstream 100 bases:

>100_bases
TGGTGTGTTGACCAAGCGTTAATAATTTTGCAAAGCCGCATACTGAAAAGTAAGCGGCTTTTTTATTGTCTATGATGAAG
GATTGACAGTAGACAGAGGC

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKFIVKPHPEIFVKSESVRKRFTKILERNIRTILQRRTESVAVFNRRDHIEVTSESDKYFKETLEVLTQTPGIHHSLEVQ
QSEFKDLHNIYEQVLERSGALIEGKTFSVRAKRRGKHDFTSIELERYVGGGLNQAVESAKVKLKNPDITVKVEVENDKLN
QVIERHKGLGGFPLGTQEDLLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVSHYLWDKYGSSAKVKFI
SIDFEPVVAEILENVEDGQMGVVLKRMFMRAGGMVAERFGIEALVTGEALGQVSSQTLTNLRHIDNVTDTLILRPLINWD
KEDIIDLSRKIGTEDFAKVMPEYCGVISKKPTVKAKKGKLEAEEAKFNFEVLEQVIENARIMDIRDIEKESQEQAPEVEQ
VQAVAEHAVVLDIRSPDEEDESPLEIDGVEIKHIPFFKLSTQFGDLDQSKEYLLYCDRGVMSRLQALYLQEQGFHNVKVY
RP

Sequences:

>Translated_482_residues
MKFIVKPHPEIFVKSESVRKRFTKILERNIRTILQRRTESVAVFNRRDHIEVTSESDKYFKETLEVLTQTPGIHHSLEVQ
QSEFKDLHNIYEQVLERSGALIEGKTFSVRAKRRGKHDFTSIELERYVGGGLNQAVESAKVKLKNPDITVKVEVENDKLN
QVIERHKGLGGFPLGTQEDLLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVSHYLWDKYGSSAKVKFI
SIDFEPVVAEILENVEDGQMGVVLKRMFMRAGGMVAERFGIEALVTGEALGQVSSQTLTNLRHIDNVTDTLILRPLINWD
KEDIIDLSRKIGTEDFAKVMPEYCGVISKKPTVKAKKGKLEAEEAKFNFEVLEQVIENARIMDIRDIEKESQEQAPEVEQ
VQAVAEHAVVLDIRSPDEEDESPLEIDGVEIKHIPFFKLSTQFGDLDQSKEYLLYCDRGVMSRLQALYLQEQGFHNVKVY
RP
>Mature_482_residues
MKFIVKPHPEIFVKSESVRKRFTKILERNIRTILQRRTESVAVFNRRDHIEVTSESDKYFKETLEVLTQTPGIHHSLEVQ
QSEFKDLHNIYEQVLERSGALIEGKTFSVRAKRRGKHDFTSIELERYVGGGLNQAVESAKVKLKNPDITVKVEVENDKLN
QVIERHKGLGGFPLGTQEDLLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVSHYLWDKYGSSAKVKFI
SIDFEPVVAEILENVEDGQMGVVLKRMFMRAGGMVAERFGIEALVTGEALGQVSSQTLTNLRHIDNVTDTLILRPLINWD
KEDIIDLSRKIGTEDFAKVMPEYCGVISKKPTVKAKKGKLEAEEAKFNFEVLEQVIENARIMDIRDIEKESQEQAPEVEQ
VQAVAEHAVVLDIRSPDEEDESPLEIDGVEIKHIPFFKLSTQFGDLDQSKEYLLYCDRGVMSRLQALYLQEQGFHNVKVY
RP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=482, Percent_Identity=62.8630705394191, Blast_Score=637, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_VIBSL (B7VJ96)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002417985.1
- ProteinModelPortal:   B7VJ96
- GeneID:   7162616
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_2401
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00450
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 54816; Mature: 54816

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 456-456 BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVKPHPEIFVKSESVRKRFTKILERNIRTILQRRTESVAVFNRRDHIEVTSESDKYF
CCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCHHHH
KETLEVLTQTPGIHHSLEVQQSEFKDLHNIYEQVLERSGALIEGKTFSVRAKRRGKHDFT
HHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCEEECCEEEEEHHHCCCCCCH
SIELERYVGGGLNQAVESAKVKLKNPDITVKVEVENDKLNQVIERHKGLGGFPLGTQEDL
HEEHHHHHCCCHHHHHHHCEEEECCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHH
LSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVSHYLWDKYGSSAKVKFI
HHHHHCCCCCCHHHEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
SIDFEPVVAEILENVEDGQMGVVLKRMFMRAGGMVAERFGIEALVTGEALGQVSSQTLTN
EECCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRHIDNVTDTLILRPLINWDKEDIIDLSRKIGTEDFAKVMPEYCGVISKKPTVKAKKGKL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EAEEAKFNFEVLEQVIENARIMDIRDIEKESQEQAPEVEQVQAVAEHAVVLDIRSPDEED
CHHHHHCCHHHHHHHHCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHCEEEEEECCCCCCC
ESPLEIDGVEIKHIPFFKLSTQFGDLDQSKEYLLYCDRGVMSRLQALYLQEQGFHNVKVY
CCCCEECCEEEEECCEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEE
RP
CC
>Mature Secondary Structure
MKFIVKPHPEIFVKSESVRKRFTKILERNIRTILQRRTESVAVFNRRDHIEVTSESDKYF
CCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCHHHH
KETLEVLTQTPGIHHSLEVQQSEFKDLHNIYEQVLERSGALIEGKTFSVRAKRRGKHDFT
HHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCEEECCEEEEEHHHCCCCCCH
SIELERYVGGGLNQAVESAKVKLKNPDITVKVEVENDKLNQVIERHKGLGGFPLGTQEDL
HEEHHHHHCCCHHHHHHHCEEEECCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCHHHH
LSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVSHYLWDKYGSSAKVKFI
HHHHHCCCCCCHHHEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
SIDFEPVVAEILENVEDGQMGVVLKRMFMRAGGMVAERFGIEALVTGEALGQVSSQTLTN
EECCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCEEEEEHHHHHHHHHHHHHH
LRHIDNVTDTLILRPLINWDKEDIIDLSRKIGTEDFAKVMPEYCGVISKKPTVKAKKGKL
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EAEEAKFNFEVLEQVIENARIMDIRDIEKESQEQAPEVEQVQAVAEHAVVLDIRSPDEED
CHHHHHCCHHHHHHHHCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHCEEEEEECCCCCCC
ESPLEIDGVEIKHIPFFKLSTQFGDLDQSKEYLLYCDRGVMSRLQALYLQEQGFHNVKVY
CCCCEECCEEEEECCEEEECCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEE
RP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA