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The map label for this gene is gcvA [H]

Identifier: 218710363

GI number: 218710363

Start: 2568878

End: 2569786

Strand: Reverse

Name: gcvA [H]

Synonym: VS_2399

Alternate gene names: 218710363

Gene position: 2569786-2568878 (Counterclockwise)

Preceding gene: 218710364

Following gene: 218710362

Centisome position: 77.89

GC content: 46.64

Gene sequence:

>909_bases
ATGTCTCGTCGATTACCCCCATTAAACTCGCTAAGAGTGTTTGAAGCCGCGGCTCGACACTTGAGTTTTACGCGTGCCGC
AGAAGAGTTGTTTGTTACTCAAGCCGCGGTCAGTCATCAGATCAAAGCGCTTGAAGAATTCTTATCTTTGAAGCTGTTTC
GTCGAAGAAACCGCTCTTTGCTGCTAACTGAAGAAGGTCAAAGCTACTTCTTAGATATCAAAGATATTTTCACATCACTG
GCAGAAGCGACAGACAAAGTGCTTGAGCGAAGTGAGAAGGGCGCATTGACCATCAGTTTACCACCGAGTTTTGCTATTCA
ATGGTTGGTACCAAGGCTCGCTGACTTTAATCAGCAAGAACCTGATATTGATGTAAGAATCAAAGCCGTTGATATGGATG
AAGGCTCGCTGACCGATGATGTAGACGTGGCTATTTACTATGGCCGAGGTAATTGGTCGGGGCTCAGAGCCGATAAGCTT
TACCAAGAATACTTGATCCCTCTTTGCTCACCTTCTGTGCTGCTAGGAGTCAAACCATTAGAATCTCTCAGTGATTTGGC
ATGTCATACGCTATTGCATGATACCTCTCGAAAGGATTGGAAACAGTTCGCTAAGCAAAATGGCATCGACGGAGTAAACG
TTAATCACGGTCCTATCTTCAGTCACTCTACCATGGTGCTGCAGGCAGCGGCTCACGGCCAGGGCATTGCTCTAGGTAAC
AACGTGTTGGCGCAGCCTGAAATCGAAGCGGGTCGTTTGATTGCACCTTTTGATGAAGTGTTGGTGAGTAAGAACGCCTT
CTATGTGGTTTGTCATGAGAAACAAGCCGACATGGGTCGTATTGCTACCTTCCGCGACTGGATGCTAGCGAAAGCGCAAA
GTGAACAAGAGGACTTACTCGATGAATAA

Upstream 100 bases:

>100_bases
GCTGCTGCGGGATGAAATATAGAATTTCTCTGGCCGCTAGGCAATGGACAAATTATAATGTTTCAGATAAAAAAAACTAA
TGCTTATTTAGAGAGTAACT

Downstream 100 bases:

>100_bases
CGTCATCATTGATGGTGAAGACAATCCGATCACCTTTATCTTTGCCCATGGTGCTGGCGCAGGCATGGATCATGAGTTCA
TGCAATCGGTAGCCAAAGGG

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSLLLTEEGQSYFLDIKDIFTSL
AEATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNQQEPDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWSGLRADKL
YQEYLIPLCSPSVLLGVKPLESLSDLACHTLLHDTSRKDWKQFAKQNGIDGVNVNHGPIFSHSTMVLQAAAHGQGIALGN
NVLAQPEIEAGRLIAPFDEVLVSKNAFYVVCHEKQADMGRIATFRDWMLAKAQSEQEDLLDE

Sequences:

>Translated_302_residues
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSLLLTEEGQSYFLDIKDIFTSL
AEATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNQQEPDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWSGLRADKL
YQEYLIPLCSPSVLLGVKPLESLSDLACHTLLHDTSRKDWKQFAKQNGIDGVNVNHGPIFSHSTMVLQAAAHGQGIALGN
NVLAQPEIEAGRLIAPFDEVLVSKNAFYVVCHEKQADMGRIATFRDWMLAKAQSEQEDLLDE
>Mature_301_residues
SRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSLLLTEEGQSYFLDIKDIFTSLA
EATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNQQEPDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWSGLRADKLY
QEYLIPLCSPSVLLGVKPLESLSDLACHTLLHDTSRKDWKQFAKQNGIDGVNVNHGPIFSHSTMVLQAAAHGQGIALGNN
VLAQPEIEAGRLIAPFDEVLVSKNAFYVVCHEKQADMGRIATFRDWMLAKAQSEQEDLLDE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=299, Percent_Identity=72.2408026755853, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=34.6153846153846, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=29.5918367346939, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=29.2253521126761, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI145693193, Length=288, Percent_Identity=26.7361111111111, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081978, Length=271, Percent_Identity=29.8892988929889, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI157672245, Length=157, Percent_Identity=31.2101910828025, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=33.0769230769231, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=25.2525252525253, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87082132, Length=159, Percent_Identity=27.0440251572327, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787601, Length=190, Percent_Identity=26.8421052631579, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789440, Length=277, Percent_Identity=25.9927797833935, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693105, Length=190, Percent_Identity=25.2631578947368, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=210, Percent_Identity=25.7142857142857, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33838; Mature: 33707

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKDIFTSLAEATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNQQE
EEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCC
PDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWSGLRADKLYQEYLIPLCSPSVLLGVKPL
CCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCH
ESLSDLACHTLLHDTSRKDWKQFAKQNGIDGVNVNHGPIFSHSTMVLQAAAHGQGIALGN
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEECCCCCCEEECC
NVLAQPEIEAGRLIAPFDEVLVSKNAFYVVCHEKQADMGRIATFRDWMLAKAQSEQEDLL
CCCCCCCCCCCCEECCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHC
DE
CC
>Mature Secondary Structure 
SRRLPPLNSLRVFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEFLSLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKDIFTSLAEATDKVLERSEKGALTISLPPSFAIQWLVPRLADFNQQE
EEEECCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCC
PDIDVRIKAVDMDEGSLTDDVDVAIYYGRGNWSGLRADKLYQEYLIPLCSPSVLLGVKPL
CCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCH
ESLSDLACHTLLHDTSRKDWKQFAKQNGIDGVNVNHGPIFSHSTMVLQAAAHGQGIALGN
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEEECCCCCCEEECC
NVLAQPEIEAGRLIAPFDEVLVSKNAFYVVCHEKQADMGRIATFRDWMLAKAQSEQEDLL
CCCCCCCCCCCCEECCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHC
DE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]