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The map label for this gene is tsf [H]

Identifier: 218710317

GI number: 218710317

Start: 2520766

End: 2521611

Strand: Reverse

Name: tsf [H]

Synonym: VS_2352

Alternate gene names: 218710317

Gene position: 2521611-2520766 (Counterclockwise)

Preceding gene: 218710318

Following gene: 218710316

Centisome position: 76.43

GC content: 45.39

Gene sequence:

>846_bases
ATGGCAACTGTAACTGCAGCTCTAGTTAAAGAACTTCGTGAACGCACAGCTGCGGGCATGATGGAATGTAAAAAAGCGCT
TGTTGCTGCTGAAGGCGACATCGAGCTAGCAATTGAAAACATGCGTAAATCTGGCGCAGCGAAAGCAGCTAAAAAAGCTG
GTAACGTTGCTGCTGAAGGCGCAATCATCATTAAAGAAGAAGCTGGCGTTGCTGCTCTTCTTGAAGTGAACTGCCAAACT
GATTTCGTAGCTAAAGATGCAGGTTTCCTTGCATTCGCTAACGAAGTTGCTGAAGTTGCTCTAGCTGAACGTCTAGACAT
CGTTGCACTTCAAGCTAAGTTTGAAGACGCACGTATCGCTCTAGTAACTAAGATCGGTGAGAACATCAGCATCCGTCGTG
TTGAGCTTGTTGAAGGTGTTGCACTAGCTTCTTACCGTCACGGCGAGAAAATCGGTGTTGTTGTTGCTGGTGAAGGCGAA
GCTGAAACGCTTAAGCACATCGCTATGCACGTTGCTGCTTCTAAGCCTGAGTACGTTAACCCATCTGACGTACCTGCTGA
CGTAGTAGAAAAAGAAAAAGCTGTTCAAGTTGAAATCGCTATGAACGAAGGCAAACCACAAGAGATCGCTGAGAAAATGG
TTATCGGCCGTATGAAGAAATTCACGGGCGAAGTATCTCTTACTGGTCAAGCTTTCATCATGGAACCTAAGAAAACTGTT
GCTGACATTCTTAAAGAGAAAGGCGCATCAGTTACTACCTTCGTTCGTTTAGAAGTTGGTGAAGGTATCGAGAAAGCAGC
TGAAATGAGCTTCGCAGACGAAGTTGCAGCGGTACAAAAAGGTTAA

Upstream 100 bases:

>100_bases
TCATAATCGATGGCTTAAGAACTGAATACAGTCAATATCAGGGGCCAATTAATTAGGCCCCTGTTTTTTATCTTTTTTAG
AATTTACTGAGGAATAGAGA

Downstream 100 bases:

>100_bases
TCCTTAGCGTATTGTTTGAAAAGAGACCGTGGCAAATGCTGCGGTCTTTTTATGAATAATGAATTATTTCCGGCTTTTAG
CTGTTATGAATGCAAACTGA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MATVTAALVKELRERTAAGMMECKKALVAAEGDIELAIENMRKSGAAKAAKKAGNVAAEGAIIIKEEAGVAALLEVNCQT
DFVAKDAGFLAFANEVAEVALAERLDIVALQAKFEDARIALVTKIGENISIRRVELVEGVALASYRHGEKIGVVVAGEGE
AETLKHIAMHVAASKPEYVNPSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTV
ADILKEKGASVTTFVRLEVGEGIEKAAEMSFADEVAAVQKG

Sequences:

>Translated_281_residues
MATVTAALVKELRERTAAGMMECKKALVAAEGDIELAIENMRKSGAAKAAKKAGNVAAEGAIIIKEEAGVAALLEVNCQT
DFVAKDAGFLAFANEVAEVALAERLDIVALQAKFEDARIALVTKIGENISIRRVELVEGVALASYRHGEKIGVVVAGEGE
AETLKHIAMHVAASKPEYVNPSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTV
ADILKEKGASVTTFVRLEVGEGIEKAAEMSFADEVAAVQKG
>Mature_280_residues
ATVTAALVKELRERTAAGMMECKKALVAAEGDIELAIENMRKSGAAKAAKKAGNVAAEGAIIIKEEAGVAALLEVNCQTD
FVAKDAGFLAFANEVAEVALAERLDIVALQAKFEDARIALVTKIGENISIRRVELVEGVALASYRHGEKIGVVVAGEGEA
ETLKHIAMHVAASKPEYVNPSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTVA
DILKEKGASVTTFVRLEVGEGIEKAAEMSFADEVAAVQKG

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Escherichia coli, GI1786366, Length=282, Percent_Identity=67.3758865248227, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17561440, Length=292, Percent_Identity=24.3150684931507, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921466, Length=284, Percent_Identity=26.7605633802817, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 29777; Mature: 29646

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVTAALVKELRERTAAGMMECKKALVAAEGDIELAIENMRKSGAAKAAKKAGNVAAEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCEECCC
AIIIKEEAGVAALLEVNCQTDFVAKDAGFLAFANEVAEVALAERLDIVALQAKFEDARIA
EEEEEECCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEE
LVTKIGENISIRRVELVEGVALASYRHGEKIGVVVAGEGEAETLKHIAMHVAASKPEYVN
EEEECCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
PSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTV
CCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEECCHHHH
ADILKEKGASVTTFVRLEVGEGIEKAAEMSFADEVAAVQKG
HHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATVTAALVKELRERTAAGMMECKKALVAAEGDIELAIENMRKSGAAKAAKKAGNVAAEG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCEECCC
AIIIKEEAGVAALLEVNCQTDFVAKDAGFLAFANEVAEVALAERLDIVALQAKFEDARIA
EEEEEECCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEE
LVTKIGENISIRRVELVEGVALASYRHGEKIGVVVAGEGEAETLKHIAMHVAASKPEYVN
EEEECCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
PSDVPADVVEKEKAVQVEIAMNEGKPQEIAEKMVIGRMKKFTGEVSLTGQAFIMEPKKTV
CCCCCHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEECCHHHH
ADILKEKGASVTTFVRLEVGEGIEKAAEMSFADEVAAVQKG
HHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA