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The map label for this gene is mcrB [H]

Identifier: 218709372

GI number: 218709372

Start: 1493937

End: 1496678

Strand: Direct

Name: mcrB [H]

Synonym: VS_1381

Alternate gene names: 218709372

Gene position: 1493937-1496678 (Clockwise)

Preceding gene: 218709371

Following gene: 218709373

Centisome position: 45.28

GC content: 40.08

Gene sequence:

>2742_bases
ATGGAAGTCGTAGAACAACAGTTGTGGAATGATTTTTTAACACGCTGGCCCCGTGAAAAGTTAAGTGATTTAACGTTGGA
GCAATATGTATCAGTTAACGATGACGACACATTCACCTATTGGTTAGAAACGAAAACCAGAGAGCTCGGATCTATTCAAG
GTAATACTTCTGCGAAATTTGGAATTTATAAACGTAATAGCGAAGGGAAAAAGCAGAGCGGTATTGACCATGGTGAGGTT
TATACGTGGCGGAATCGTTATGGTAATAACGAATCAATGGTTTTTGACTACGTTAAAAATGTCTTAATCCAGGTTGCACA
AGCGGCTTACGAAGGCGACTTGGACAAAATAGATCTTATAGACTTTGCTCCCTTAGTTAAATGGAAGATTGCTTTCCTAT
ATCAAGATCAAGATAAGCCTGTATTAATCAATACTTTTTCAAAGCCTATGTTAGAGGTGTTGACCAGCTCTAACAACAAG
GTGTCGTTTCCTCAAATGTATCAGCAGCTTATTCTCGAAAAAGGTGAACAAGATTTGTTGGAGTTTGGTGATGCTTGTTG
GAAGAAAGCAGAGCATAAACGTAAAGAAATTCTTCAGCGGACTATTTTTGACCAATTCTCTCATATTGAATTGTTTAATC
GCGGCTTGGAACAGTGGCCGTTGGATACTATTGATGCATTCTGCTCGTTAATTACAGAAGCGAATAGCCACAAATTAGAT
GTCTTCACTACATCTATGTCAACAGGTGCAATGATTCGAATCGGTCGAAAAGAACTTGGAGCAGCTAAAGCAGACGAAGT
TTTCGCTACATTTGAACCGACACTGAAAAAAATCAACTTTGAGCTTCGCTATGAGCATCGAGATGGTTACGAGTGCGCTG
AGGTTTCTGAGGAGTTATATCAACGTACACGCAAATCAAAAAAATTGGCGCAATTCGCAAAACAATATTCAATTAAACGT
AAGGCGCATTGGCCTAAGAGTTACCTGAATGATAACGCAAGCGATGTTGAATATGACGAGTATCAGATAGCTGAAGCAAG
AGAGAAATACGTAATGCAGAACCAGTCACCACTAAATCAAATTCTTTATGGCCCACCGGGAACCGGAAAAACCTACCACA
CTATTGAAGCGGCCGTTAAAGCCGCTGAACCTGAGTTTTCTTGGGAAACACGCGGTGAGCTAAAAGCCAAATACGATGAA
CTGGTTACGGCTAAACGAATTCGTTTTGTTACGTTCCACCAGAGCTACGGTTATGAAGAGTTTGTTGAGGGGTTAAAAGC
GAAAACAACGGAAGAAAAGTCCATTGAATATGTTGTTGAAAATGGTGTTTTTAAGGAAATAGTTGAAGATGCAAAAAGCA
GTGATTTGCGAAAAGCGTTTGAAGTTAATGCGGATGCAACAATATGGAAGATTTCAATCGATGGTACTGGTCAAAGTGTG
GTGTCAAACTATTGTTTAGCGAATGGTCTTGCAGCAATCGGTTGGGGAGAGGCTGGAGAGCTCCTATCTGAAGATTTAGA
TAACAATGAGTACTATCAAAAGCTTGGTCCTCAAGTGAAAAGCTCTCTATCCGAGTTCTCTCAACGTTCGTCGGCTGGAG
ACCTTATTCTGTGTATTGGTAGCCAACGCACTGTTCAGGCTGTAGGGGTGATTACTGGAGATTACCATTTTGAACAAGCA
GGTACCTCAGAGTACGATCATTACTGTAATCAGTTACCAGTGAATTGGTTGGTAACCGATATTGATGTCGATTTTCATGA
GTTGAATGGTGGTGTGAACTTTACACAAAAAACATTCTATGAACTTTGGCGATTCTCCGTGTCTGATGTGTTCGACTTAT
TGAAGAAGCAAGGGATTGATATAAACCCTGTGAAAGCAGAACGAGAAGTAGAAAATTATGTTTTAGTTATCGACGAAATC
AACCGCGGCAATATTTCTAAAATATTTGGTGAATTGATAACCCTAATCGAACCAAGTAAGCGAATGCCAGCGGAAAGAAT
ATCGACTGACGAATGCTTGGAAATTAGCCTGCCGCATAGTATTCCGCTGTTTTCTGTGCCAGATAACTTGTACCTAATCG
GCACTATGAATACGGCTGACCGCTCGTTAGCTATGATGGATACGGCACTTCGCCGTCGTTTTGATTTCGTCGAAATGATG
CCTAAGCCAGAGTTGTTTGGAAATAAAACAGTGAAGGGAATTGATCTTACCCAGTTACTTGAAACCTTAAATGAGCGCAT
TGAAATTTTATACGACCGAGAACACACACTTGGCCATGCGTTTTTGTTTCCAGCTTTTAACGCCGAAACAGAAGATGAGG
CATTTAAAGAACTACAGTTGGCGTTTAAGAACAAGATCATTCCACTTCTAGAAGAATACTTCTTTGAAGACTGGGATAAA
ATCCGTTTGGTATTAGCGGACAACCAAAAATTGGAAGGTAAGAAACCAGAAAGTAAGGCAGAGCTCGAATTAGCAAAGCT
ACAGTTTGTTGTTGAGAAAGATCTTAGCCCGAAGGAGCTTACTAACCTATTTGGCACTAAACATAACCTAAATCAGTACG
ATGAAGTAAAACAATATTTCTTAAAAGACAAAGCAGATGATGTTTGGAAAGAACCTAATGCTTATAAAGCTATATACGCG
CTTGATGAAGTGAAACGTGCCTTGTTGAAGGCTAATAATGACAATCAAGAACAAAGCGATAATACCGAAAATCAGCTGAG
TTCTGAGGGCGTGGTTGATTAA

Upstream 100 bases:

>100_bases
GTGCTAAGTCAAAGACCTTATGGTTATGAAATGATAGGCTTATTTTATTCTTATTCATCACTATTGAATTATATAAAAAT
TATAAATAACGGTAGAAATT

Downstream 100 bases:

>100_bases
CATGACCGTTCCCTTTACTGTTTTTGAATTTGGCTACTTAACTTGCGACGAACACGCTGCGCAAGCCAATGGTTACACGC
TTATTTCAGCCAATGCGTTT

Product: hypothetical protein

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 913; Mature: 913

Protein sequence:

>913_residues
MEVVEQQLWNDFLTRWPREKLSDLTLEQYVSVNDDDTFTYWLETKTRELGSIQGNTSAKFGIYKRNSEGKKQSGIDHGEV
YTWRNRYGNNESMVFDYVKNVLIQVAQAAYEGDLDKIDLIDFAPLVKWKIAFLYQDQDKPVLINTFSKPMLEVLTSSNNK
VSFPQMYQQLILEKGEQDLLEFGDACWKKAEHKRKEILQRTIFDQFSHIELFNRGLEQWPLDTIDAFCSLITEANSHKLD
VFTTSMSTGAMIRIGRKELGAAKADEVFATFEPTLKKINFELRYEHRDGYECAEVSEELYQRTRKSKKLAQFAKQYSIKR
KAHWPKSYLNDNASDVEYDEYQIAEAREKYVMQNQSPLNQILYGPPGTGKTYHTIEAAVKAAEPEFSWETRGELKAKYDE
LVTAKRIRFVTFHQSYGYEEFVEGLKAKTTEEKSIEYVVENGVFKEIVEDAKSSDLRKAFEVNADATIWKISIDGTGQSV
VSNYCLANGLAAIGWGEAGELLSEDLDNNEYYQKLGPQVKSSLSEFSQRSSAGDLILCIGSQRTVQAVGVITGDYHFEQA
GTSEYDHYCNQLPVNWLVTDIDVDFHELNGGVNFTQKTFYELWRFSVSDVFDLLKKQGIDINPVKAEREVENYVLVIDEI
NRGNISKIFGELITLIEPSKRMPAERISTDECLEISLPHSIPLFSVPDNLYLIGTMNTADRSLAMMDTALRRRFDFVEMM
PKPELFGNKTVKGIDLTQLLETLNERIEILYDREHTLGHAFLFPAFNAETEDEAFKELQLAFKNKIIPLLEEYFFEDWDK
IRLVLADNQKLEGKKPESKAELELAKLQFVVEKDLSPKELTNLFGTKHNLNQYDEVKQYFLKDKADDVWKEPNAYKAIYA
LDEVKRALLKANNDNQEQSDNTENQLSSEGVVD

Sequences:

>Translated_913_residues
MEVVEQQLWNDFLTRWPREKLSDLTLEQYVSVNDDDTFTYWLETKTRELGSIQGNTSAKFGIYKRNSEGKKQSGIDHGEV
YTWRNRYGNNESMVFDYVKNVLIQVAQAAYEGDLDKIDLIDFAPLVKWKIAFLYQDQDKPVLINTFSKPMLEVLTSSNNK
VSFPQMYQQLILEKGEQDLLEFGDACWKKAEHKRKEILQRTIFDQFSHIELFNRGLEQWPLDTIDAFCSLITEANSHKLD
VFTTSMSTGAMIRIGRKELGAAKADEVFATFEPTLKKINFELRYEHRDGYECAEVSEELYQRTRKSKKLAQFAKQYSIKR
KAHWPKSYLNDNASDVEYDEYQIAEAREKYVMQNQSPLNQILYGPPGTGKTYHTIEAAVKAAEPEFSWETRGELKAKYDE
LVTAKRIRFVTFHQSYGYEEFVEGLKAKTTEEKSIEYVVENGVFKEIVEDAKSSDLRKAFEVNADATIWKISIDGTGQSV
VSNYCLANGLAAIGWGEAGELLSEDLDNNEYYQKLGPQVKSSLSEFSQRSSAGDLILCIGSQRTVQAVGVITGDYHFEQA
GTSEYDHYCNQLPVNWLVTDIDVDFHELNGGVNFTQKTFYELWRFSVSDVFDLLKKQGIDINPVKAEREVENYVLVIDEI
NRGNISKIFGELITLIEPSKRMPAERISTDECLEISLPHSIPLFSVPDNLYLIGTMNTADRSLAMMDTALRRRFDFVEMM
PKPELFGNKTVKGIDLTQLLETLNERIEILYDREHTLGHAFLFPAFNAETEDEAFKELQLAFKNKIIPLLEEYFFEDWDK
IRLVLADNQKLEGKKPESKAELELAKLQFVVEKDLSPKELTNLFGTKHNLNQYDEVKQYFLKDKADDVWKEPNAYKAIYA
LDEVKRALLKANNDNQEQSDNTENQLSSEGVVD
>Mature_913_residues
MEVVEQQLWNDFLTRWPREKLSDLTLEQYVSVNDDDTFTYWLETKTRELGSIQGNTSAKFGIYKRNSEGKKQSGIDHGEV
YTWRNRYGNNESMVFDYVKNVLIQVAQAAYEGDLDKIDLIDFAPLVKWKIAFLYQDQDKPVLINTFSKPMLEVLTSSNNK
VSFPQMYQQLILEKGEQDLLEFGDACWKKAEHKRKEILQRTIFDQFSHIELFNRGLEQWPLDTIDAFCSLITEANSHKLD
VFTTSMSTGAMIRIGRKELGAAKADEVFATFEPTLKKINFELRYEHRDGYECAEVSEELYQRTRKSKKLAQFAKQYSIKR
KAHWPKSYLNDNASDVEYDEYQIAEAREKYVMQNQSPLNQILYGPPGTGKTYHTIEAAVKAAEPEFSWETRGELKAKYDE
LVTAKRIRFVTFHQSYGYEEFVEGLKAKTTEEKSIEYVVENGVFKEIVEDAKSSDLRKAFEVNADATIWKISIDGTGQSV
VSNYCLANGLAAIGWGEAGELLSEDLDNNEYYQKLGPQVKSSLSEFSQRSSAGDLILCIGSQRTVQAVGVITGDYHFEQA
GTSEYDHYCNQLPVNWLVTDIDVDFHELNGGVNFTQKTFYELWRFSVSDVFDLLKKQGIDINPVKAEREVENYVLVIDEI
NRGNISKIFGELITLIEPSKRMPAERISTDECLEISLPHSIPLFSVPDNLYLIGTMNTADRSLAMMDTALRRRFDFVEMM
PKPELFGNKTVKGIDLTQLLETLNERIEILYDREHTLGHAFLFPAFNAETEDEAFKELQLAFKNKIIPLLEEYFFEDWDK
IRLVLADNQKLEGKKPESKAELELAKLQFVVEKDLSPKELTNLFGTKHNLNQYDEVKQYFLKDKADDVWKEPNAYKAIYA
LDEVKRALLKANNDNQEQSDNTENQLSSEGVVD

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=202, Percent_Identity=35.6435643564356, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 105107; Mature: 105107

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVVEQQLWNDFLTRWPREKLSDLTLEQYVSVNDDDTFTYWLETKTRELGSIQGNTSAKF
CCHHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCEEEEEECHHHHHCCCCCCCCCEE
GIYKRNSEGKKQSGIDHGEVYTWRNRYGNNESMVFDYVKNVLIQVAQAAYEGDLDKIDLI
EEEECCCCCCHHCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
DFAPLVKWKIAFLYQDQDKPVLINTFSKPMLEVLTSSNNKVSFPQMYQQLILEKGEQDLL
HHHHHHHEEEEEEEECCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHH
EFGDACWKKAEHKRKEILQRTIFDQFSHIELFNRGLEQWPLDTIDAFCSLITEANSHKLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCEEE
VFTTSMSTGAMIRIGRKELGAAKADEVFATFEPTLKKINFELRYEHRDGYECAEVSEELY
EEEECCCCCCEEEECHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHH
QRTRKSKKLAQFAKQYSIKRKAHWPKSYLNDNASDVEYDEYQIAEAREKYVMQNQSPLNQ
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
ILYGPPGTGKTYHTIEAAVKAAEPEFSWETRGELKAKYDELVTAKRIRFVTFHQSYGYEE
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCHHH
FVEGLKAKTTEEKSIEYVVENGVFKEIVEDAKSSDLRKAFEVNADATIWKISIDGTGQSV
HHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHH
VSNYCLANGLAAIGWGEAGELLSEDLDNNEYYQKLGPQVKSSLSEFSQRSSAGDLILCIG
HHHHHHHCCCCEECCCCHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEC
SQRTVQAVGVITGDYHFEQAGTSEYDHYCNQLPVNWLVTDIDVDFHELNGGVNFTQKTFY
CCCEEEEEEEEECCCEECCCCCCHHHHHHHCCCCCEEEEEECCCHHHCCCCCCHHHHHHH
ELWRFSVSDVFDLLKKQGIDINPVKAEREVENYVLVIDEINRGNISKIFGELITLIEPSK
HHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
RMPAERISTDECLEISLPHSIPLFSVPDNLYLIGTMNTADRSLAMMDTALRRRFDFVEMM
CCCHHHCCCCCEEEEECCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
PKPELFGNKTVKGIDLTQLLETLNERIEILYDREHTLGHAFLFPAFNAETEDEAFKELQL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
AFKNKIIPLLEEYFFEDWDKIRLVLADNQKLEGKKPESKAELELAKLQFVVEKDLSPKEL
HHHHCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
TNLFGTKHNLNQYDEVKQYFLKDKADDVWKEPNAYKAIYALDEVKRALLKANNDNQEQSD
HHHHCCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
NTENQLSSEGVVD
CCHHHHCCCCCCC
>Mature Secondary Structure
MEVVEQQLWNDFLTRWPREKLSDLTLEQYVSVNDDDTFTYWLETKTRELGSIQGNTSAKF
CCHHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCCEEEEEECHHHHHCCCCCCCCCEE
GIYKRNSEGKKQSGIDHGEVYTWRNRYGNNESMVFDYVKNVLIQVAQAAYEGDLDKIDLI
EEEECCCCCCHHCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHH
DFAPLVKWKIAFLYQDQDKPVLINTFSKPMLEVLTSSNNKVSFPQMYQQLILEKGEQDLL
HHHHHHHEEEEEEEECCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHH
EFGDACWKKAEHKRKEILQRTIFDQFSHIELFNRGLEQWPLDTIDAFCSLITEANSHKLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCEEE
VFTTSMSTGAMIRIGRKELGAAKADEVFATFEPTLKKINFELRYEHRDGYECAEVSEELY
EEEECCCCCCEEEECHHHHCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHH
QRTRKSKKLAQFAKQYSIKRKAHWPKSYLNDNASDVEYDEYQIAEAREKYVMQNQSPLNQ
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
ILYGPPGTGKTYHTIEAAVKAAEPEFSWETRGELKAKYDELVTAKRIRFVTFHQSYGYEE
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEEECCCCHHH
FVEGLKAKTTEEKSIEYVVENGVFKEIVEDAKSSDLRKAFEVNADATIWKISIDGTGQSV
HHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHH
VSNYCLANGLAAIGWGEAGELLSEDLDNNEYYQKLGPQVKSSLSEFSQRSSAGDLILCIG
HHHHHHHCCCCEECCCCHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEC
SQRTVQAVGVITGDYHFEQAGTSEYDHYCNQLPVNWLVTDIDVDFHELNGGVNFTQKTFY
CCCEEEEEEEEECCCEECCCCCCHHHHHHHCCCCCEEEEEECCCHHHCCCCCCHHHHHHH
ELWRFSVSDVFDLLKKQGIDINPVKAEREVENYVLVIDEINRGNISKIFGELITLIEPSK
HHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
RMPAERISTDECLEISLPHSIPLFSVPDNLYLIGTMNTADRSLAMMDTALRRRFDFVEMM
CCCHHHCCCCCEEEEECCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
PKPELFGNKTVKGIDLTQLLETLNERIEILYDREHTLGHAFLFPAFNAETEDEAFKELQL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
AFKNKIIPLLEEYFFEDWDKIRLVLADNQKLEGKKPESKAELELAKLQFVVEKDLSPKEL
HHHHCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
TNLFGTKHNLNQYDEVKQYFLKDKADDVWKEPNAYKAIYALDEVKRALLKANNDNQEQSD
HHHHCCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
NTENQLSSEGVVD
CCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]