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The map label for this gene is prpB [H]

Identifier: 218709334

GI number: 218709334

Start: 1452477

End: 1453373

Strand: Direct

Name: prpB [H]

Synonym: VS_1343

Alternate gene names: 218709334

Gene position: 1452477-1453373 (Clockwise)

Preceding gene: 218709333

Following gene: 218709335

Centisome position: 44.02

GC content: 48.05

Gene sequence:

>897_bases
ATGAAGTTATCAGCAGGAGCTAAGTTCCGACAAGCTGTGCAAGACAACGACCCATTGCAGATCGTCGGTACGGTGAATCC
GTATTGCGCGATGATGGCAAAGAACTTGGGGCATCAAGCGATCTATTTGTCTGGCGGAGGCATCGCCAATGCATCTTACG
GTTTGCCTGATTTGGGTATCACTACATTGAACGATGTGTTGGTGGATGTTGAACGTATTGCCAATGCGTGTGATCTGCCA
TTGCTGGTGGATATCGATACGGGATTTGGTGGCGCATTTAATATTGCACGTACCATTCGAGCTATGGAAAAAGCGGGCGC
AGCTGCAATTCACATGGAAGACCAAGTCGCCCAGAAACGCTGTGGTCATCGACCAAATAAAGCGATTGTAAGCCAGCAAG
AGATGGTCGATAGAGTCAAAGCGGCAACCGATGCTAGAACCGATGAGAGCTTTGTGATTATGGCGCGTACCGATGCATTA
GCGGTTGAAGGCATTGATAGTGCGATTGAAAGGGCGATTGCTTGTGTTGAAGCGGGCGCTGACATGATTTTCCCTGAGGC
GATGAATCAACTCGATCAATACGTGAAGTTCTCGGCAGCGCTGAAATCAGCAACGGGCAAGCATGTACCTATTCTCGCGA
ACATCACTGAGTTTGGCCAAACGCCGCTCTATAACTGTAACGAGTTGGCCCAATCAAGTGTCGACATGGTGCTTTATCCA
TTGAGTGCGTTCCGTGCGATGAACAAAGCGGCGGAGAATGTTTACAAGCACTTGTTAGTTGAAGGCAATCAAGAAGCTCT
GTTGGATTCAATGCAAACCCGTAAAGAGCTTTACGAACACCTGAATTACCACGACTACGAGAACAAGCTTGATCAGTTGT
TTTCAAGCGAAGGGTAA

Upstream 100 bases:

>100_bases
TTAACTGGCGACTGGTATTTGCCAGCGGTTCTTCGCTGGCGAGAGCTAAACATCAGTAGTCATAAAGATTAGAAATTAAC
AATTAACAGGGAGCGTCATT

Downstream 100 bases:

>100_bases
ACCAGTTTCAATTAATCAAAAATCAACTTAATCCAATAACGTGAAATGGTTAGTCGCCATATCCAGTACCGATGATAAAG
ATCATCGGACATATAAACAG

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MKLSAGAKFRQAVQDNDPLQIVGTVNPYCAMMAKNLGHQAIYLSGGGIANASYGLPDLGITTLNDVLVDVERIANACDLP
LLVDIDTGFGGAFNIARTIRAMEKAGAAAIHMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAATDARTDESFVIMARTDAL
AVEGIDSAIERAIACVEAGADMIFPEAMNQLDQYVKFSAALKSATGKHVPILANITEFGQTPLYNCNELAQSSVDMVLYP
LSAFRAMNKAAENVYKHLLVEGNQEALLDSMQTRKELYEHLNYHDYENKLDQLFSSEG

Sequences:

>Translated_298_residues
MKLSAGAKFRQAVQDNDPLQIVGTVNPYCAMMAKNLGHQAIYLSGGGIANASYGLPDLGITTLNDVLVDVERIANACDLP
LLVDIDTGFGGAFNIARTIRAMEKAGAAAIHMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAATDARTDESFVIMARTDAL
AVEGIDSAIERAIACVEAGADMIFPEAMNQLDQYVKFSAALKSATGKHVPILANITEFGQTPLYNCNELAQSSVDMVLYP
LSAFRAMNKAAENVYKHLLVEGNQEALLDSMQTRKELYEHLNYHDYENKLDQLFSSEG
>Mature_298_residues
MKLSAGAKFRQAVQDNDPLQIVGTVNPYCAMMAKNLGHQAIYLSGGGIANASYGLPDLGITTLNDVLVDVERIANACDLP
LLVDIDTGFGGAFNIARTIRAMEKAGAAAIHMEDQVAQKRCGHRPNKAIVSQQEMVDRVKAATDARTDESFVIMARTDAL
AVEGIDSAIERAIACVEAGADMIFPEAMNQLDQYVKFSAALKSATGKHVPILANITEFGQTPLYNCNELAQSSVDMVLYP
LSAFRAMNKAAENVYKHLLVEGNQEALLDSMQTRKELYEHLNYHDYENKLDQLFSSEG

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=293, Percent_Identity=63.1399317406143, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1790445, Length=167, Percent_Identity=34.7305389221557, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32447; Mature: 32447

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSAGAKFRQAVQDNDPLQIVGTVNPYCAMMAKNLGHQAIYLSGGGIANASYGLPDLGI
CCCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCH
TTLNDVLVDVERIANACDLPLLVDIDTGFGGAFNIARTIRAMEKAGAAAIHMEDQVAQKR
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHH
CGHRPNKAIVSQQEMVDRVKAATDARTDESFVIMARTDALAVEGIDSAIERAIACVEAGA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC
DMIFPEAMNQLDQYVKFSAALKSATGKHVPILANITEFGQTPLYNCNELAQSSVDMVLYP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHCCCCCCCHHHHHHCCHHHEEHH
LSAFRAMNKAAENVYKHLLVEGNQEALLDSMQTRKELYEHLNYHDYENKLDQLFSSEG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKLSAGAKFRQAVQDNDPLQIVGTVNPYCAMMAKNLGHQAIYLSGGGIANASYGLPDLGI
CCCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCH
TTLNDVLVDVERIANACDLPLLVDIDTGFGGAFNIARTIRAMEKAGAAAIHMEDQVAQKR
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHH
CGHRPNKAIVSQQEMVDRVKAATDARTDESFVIMARTDALAVEGIDSAIERAIACVEAGA
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCC
DMIFPEAMNQLDQYVKFSAALKSATGKHVPILANITEFGQTPLYNCNELAQSSVDMVLYP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHCCCCCCCHHHHHHCCHHHEEHH
LSAFRAMNKAAENVYKHLLVEGNQEALLDSMQTRKELYEHLNYHDYENKLDQLFSSEG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]