Click here to switch to the map view.

The map label for this gene is prpC [H]

Identifier: 218709335

GI number: 218709335

Start: 1453520

End: 1454722

Strand: Direct

Name: prpC [H]

Synonym: VS_1344

Alternate gene names: 218709335

Gene position: 1453520-1454722 (Clockwise)

Preceding gene: 218709334

Following gene: 218709336

Centisome position: 44.06

GC content: 47.96

Gene sequence:

>1203_bases
ATGTCTGTATCTTTGAGTGATAAAGCAACTGTCGAAAAGGCTTCTACAGAAAACCAACAACAAAGAGAAAAAACAGCCAG
CACACCTGCAATCGGTGGTGCTGGTCTACGTGGCCAGAGTGCGGGAACTACGGCGCTATGTACGGTAGGCCAGTCAGGAA
CGGGTTTGACCTACCGTGGCTATGATATAACCGACCTTGCTAATCATGCTCAGTTCGAAGAAGTGGCGCATCTGCTACTA
AGAGGTCATTTACCCAGTGAAAAAGAGCTTGATGATTACAAAACATTGTTGGTTGGTCTGCGCGGCTTGCCTCAACCTTT
GAAAGTAGCATTAGAGCTTATCCCTGCTGACGCTCATCCAATGGATGTGATGAGAACGGGCTGTTCAATGTTGGGTAACT
TAGAGCAAGAATCGGACTTCTCTGAGCAACTATCAGCAACAGAACGTATGCTTGCACTTTTTCCTGCGATTATTTGTTAC
TGGTATCGCTTTAGCCATGATGGCGTGCGCATTGATACGGAAGATCAAAGCGAAGCCTGTTTGGGTGGCTATTTCTTGAA
AATGCTAACGGATAAAGCGCCGAGTGAACTTCATAAGAAGGTGATGCACTGCTCGCTAACCCTTTACGCGGAACATGAGT
TTAATGCTTCGACTTTTGCAGCCCGTGTGTGTGCATCAACGTTGTCGGATATTCACTCGTGTGTCACAGCAGCGATTGGT
ACGTTGCGAGGCCCTTTACATGGCGGTGCAAACGAAGCTGCGATGGAAATGATCCAAGATTGGCAAACCGCCGATGAAGC
GGAAGCGAACATCATGAATATGCTCGCCAACAAAGACAAAATCATGGGCTTCGGGCATGCCATTTATCGTGAGAGCGACC
CACGAAACGCCTTAATCAAACGCTGGTCGAAAGAGCTAGCTCAAGAGGTGGGTGATGAACAGCTTTATGCCGTTTCAGAG
CGTGTTGAAGCGGTTATGAAGCGAGAGAAAGGCTTGTTCTGTAATGCTGACTTCTTCCATGCGTCGGCTTATCACTTCAT
GGACATCCCAACCAAACTGTTTACGCCAATCTTTGTGATGAGCCGCCTCACAGGTTGGACGGCACACGTGTTCGAACAAA
GAGAAAACAATCGCATCATTCGTCCAAGTGCAGACTACACCGGGCCAGAGCACCAAGACTGGCTACCTATCCATTTACGT
TAG

Upstream 100 bases:

>100_bases
TGGTTAGTCGCCATATCCAGTACCGATGATAAAGATCATCGGACATATAAACAGGCAGCACCAAAGAGTGCAGGCGGCTA
ACACAGAAAAGGAGTTCAAC

Downstream 100 bases:

>100_bases
TCCTCTATATCGAGCCGACTTAAATCTCGAGGTGACTGTTATCTTCATGCGATAAGGCCTCGGGAACCAACGAATAGAAT
TTAACAGATAATGGTGGATG

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSVSLSDKATVEKASTENQQQREKTASTPAIGGAGLRGQSAGTTALCTVGQSGTGLTYRGYDITDLANHAQFEEVAHLLL
RGHLPSEKELDDYKTLLVGLRGLPQPLKVALELIPADAHPMDVMRTGCSMLGNLEQESDFSEQLSATERMLALFPAIICY
WYRFSHDGVRIDTEDQSEACLGGYFLKMLTDKAPSELHKKVMHCSLTLYAEHEFNASTFAARVCASTLSDIHSCVTAAIG
TLRGPLHGGANEAAMEMIQDWQTADEAEANIMNMLANKDKIMGFGHAIYRESDPRNALIKRWSKELAQEVGDEQLYAVSE
RVEAVMKREKGLFCNADFFHASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRENNRIIRPSADYTGPEHQDWLPIHLR

Sequences:

>Translated_400_residues
MSVSLSDKATVEKASTENQQQREKTASTPAIGGAGLRGQSAGTTALCTVGQSGTGLTYRGYDITDLANHAQFEEVAHLLL
RGHLPSEKELDDYKTLLVGLRGLPQPLKVALELIPADAHPMDVMRTGCSMLGNLEQESDFSEQLSATERMLALFPAIICY
WYRFSHDGVRIDTEDQSEACLGGYFLKMLTDKAPSELHKKVMHCSLTLYAEHEFNASTFAARVCASTLSDIHSCVTAAIG
TLRGPLHGGANEAAMEMIQDWQTADEAEANIMNMLANKDKIMGFGHAIYRESDPRNALIKRWSKELAQEVGDEQLYAVSE
RVEAVMKREKGLFCNADFFHASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRENNRIIRPSADYTGPEHQDWLPIHLR
>Mature_399_residues
SVSLSDKATVEKASTENQQQREKTASTPAIGGAGLRGQSAGTTALCTVGQSGTGLTYRGYDITDLANHAQFEEVAHLLLR
GHLPSEKELDDYKTLLVGLRGLPQPLKVALELIPADAHPMDVMRTGCSMLGNLEQESDFSEQLSATERMLALFPAIICYW
YRFSHDGVRIDTEDQSEACLGGYFLKMLTDKAPSELHKKVMHCSLTLYAEHEFNASTFAARVCASTLSDIHSCVTAAIGT
LRGPLHGGANEAAMEMIQDWQTADEAEANIMNMLANKDKIMGFGHAIYRESDPRNALIKRWSKELAQEVGDEQLYAVSER
VEAVMKREKGLFCNADFFHASAYHFMDIPTKLFTPIFVMSRLTGWTAHVFEQRENNRIIRPSADYTGPEHQDWLPIHLR

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=413, Percent_Identity=25.4237288135593, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=51.0869565217391, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI1786939, Length=362, Percent_Identity=30.939226519337, Blast_Score=163, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6319850, Length=398, Percent_Identity=22.3618090452261, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324328, Length=299, Percent_Identity=24.4147157190635, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24640124, Length=295, Percent_Identity=24.406779661017, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24640126, Length=295, Percent_Identity=24.406779661017, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356863, Length=358, Percent_Identity=22.9050279329609, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 44476; Mature: 44345

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSLSDKATVEKASTENQQQREKTASTPAIGGAGLRGQSAGTTALCTVGQSGTGLTYRG
CCCCCCCCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECC
YDITDLANHAQFEEVAHLLLRGHLPSEKELDDYKTLLVGLRGLPQPLKVALELIPADAHP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
MDVMRTGCSMLGNLEQESDFSEQLSATERMLALFPAIICYWYRFSHDGVRIDTEDQSEAC
HHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHH
LGGYFLKMLTDKAPSELHKKVMHCSLTLYAEHEFNASTFAARVCASTLSDIHSCVTAAIG
HHHHHHHHHHCCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TLRGPLHGGANEAAMEMIQDWQTADEAEANIMNMLANKDKIMGFGHAIYRESDPRNALIK
HHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCHHHHCCCCCHHHHHH
RWSKELAQEVGDEQLYAVSERVEAVMKREKGLFCNADFFHASAYHFMDIPTKLFTPIFVM
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCHHHHCCCHHHHCCCHHHHHHHHHH
SRLTGWTAHVFEQRENNRIIRPSADYTGPEHQDWLPIHLR
HHHCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
SVSLSDKATVEKASTENQQQREKTASTPAIGGAGLRGQSAGTTALCTVGQSGTGLTYRG
CCCCCCCHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEECC
YDITDLANHAQFEEVAHLLLRGHLPSEKELDDYKTLLVGLRGLPQPLKVALELIPADAHP
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
MDVMRTGCSMLGNLEQESDFSEQLSATERMLALFPAIICYWYRFSHDGVRIDTEDQSEAC
HHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHH
LGGYFLKMLTDKAPSELHKKVMHCSLTLYAEHEFNASTFAARVCASTLSDIHSCVTAAIG
HHHHHHHHHHCCCHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TLRGPLHGGANEAAMEMIQDWQTADEAEANIMNMLANKDKIMGFGHAIYRESDPRNALIK
HHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHCCCHHHHCCCCCHHHHHH
RWSKELAQEVGDEQLYAVSERVEAVMKREKGLFCNADFFHASAYHFMDIPTKLFTPIFVM
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCHHHHCCCHHHHCCCHHHHHHHHHH
SRLTGWTAHVFEQRENNRIIRPSADYTGPEHQDWLPIHLR
HHHCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]