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The map label for this gene is citS [H]

Identifier: 218709293

GI number: 218709293

Start: 1399928

End: 1401583

Strand: Direct

Name: citS [H]

Synonym: VS_1302

Alternate gene names: 218709293

Gene position: 1399928-1401583 (Clockwise)

Preceding gene: 218709286

Following gene: 218709294

Centisome position: 42.43

GC content: 44.44

Gene sequence:

>1656_bases
ATGAAATGGAGCAGTATCAGTTTCCGTAAAAGGTTACTGATTATCATGACGGTCACTGGCCTTGTTGAGCTTTTGATACT
CTCAGGTGCTGGTTTTTACTACATCAAACAATCTCAAGAGCAAGAGATGGGTTTGAAAGCGTTAGGTGTTGCTGAATTTC
TATCTGAATCGCCACTTGTCACTAGCATTATTCAAGAAAATGGAACGTTGGATCCTAACGGCGTTCCCTACGTTCTGTCT
GAAGAAACCCAAGTTAAGTTTCGTAACCTTACCCAACTCATTGGTGCGGCGTTTATCGTTGTGGGAGATGACAAAGGTAT
CCGCCTGATTCACCCTTATGATGACAGGCTCGGTAAACCAATGCGTGGTGGCGATAACCAAAAAGCGTTGGTATTGGGCG
AGTCTTATGTGTCGACGGCTAAAGGTTCACTTGGCTTCTCTGTCCGTGGGAAATCCGCGGTTAAAGACCCACAAGGCACT
GTCATTGGCGTGGTGTCGGTTGGCTACCTCTTAGATTCTTTACAAGATCGAATTGAACCTTACTTAGCCTTTTTGATTGT
GATGGCCTTGCTGGTGGTTGCGGTTAATGCTGTGATTTCAAGCTATGTATCTCGTCGTTTTCAACGCGCTATTTTGGGTT
TTGAGCCTGAAGAGATTGGCCGTTTATACGTAGAACTTGATGTGACCATGAGTACTGTGAAAGAGGGCATTTTAAGTATC
GATAAGAGCGGTATTTTGCGTTCAATTAATAAGAGTGCCTGCGATATTTTATCGATAGACCGAGATAAAGCACTTAATCA
ACAGCGCCTTTCTGATGTATTGCCAGGCAGTGATTTAGAGCAGCTGCTATCAACGGGTGACACTGATCATGATGTTGAGC
TGTACCTGAACAATAAGCGAATCATTGCTAATCGCAGCCCAATTATAGTGGCTGGTGAGGTGGTAGGGGCGGTATCCAGC
TTCCGATTGCGAGATGAAATCAACGATTTAACAGACCAGCTCTCACAAACCAAAGAGTATGCTGACCTACTGCGTTCGCA
AACCCATGAGCATCAAAATAAGCTAAACACCATTAGTGGCTTGATTCAAATGGGCGAGCTGGACTCTGTTCAACAATTGA
TTGGTCAAGAAACCGCTCATTACCAGAGCTTAATTGAGTTCTTAAGAGAGACGGTGAAAGATCCATTGATTGCAGGCATG
CTTTTAGGCAAAACAGAGCGAGCTCGAGAGATTGGTTTGGAGCTTAAAGTTGAAGAGGGGTCAAGGCTTGAGGCATTACC
CGCTTGGTTAAACGCAGAGGATGTCGTGACGATTCTTGGCAACCTGATTGATAACGCGTTTGATGCAACCATGATGGCAA
TTAAACAAGAAGGGCCAATAGCACCAGCTCGTCGCGTAATCGATGTGTCGATCAGTGATTTTGGCAATGAAATTATCGTA
GAAGTAGAAGACAAGGGCTGTGGTTTGCCGAAAGACTTGGAGACCAAAGCACTCACCGAAAAAGGCGTATCGAGCAAAGC
GAAGCAGAACAGAGGTGTTGGTTTGTATCTCATTAAGCAACTTGCAGATCGTTATCAAGGGCAATTAGAAATGGTTGATA
ACCCTGATTTTGGGTCACGCATGACGGTGTATTTACCGAAAGAAGAACAACAATAA

Upstream 100 bases:

>100_bases
ATTATTTAGTTGAGTAGCAGTCACAAATTCACGTACCTAGAAAGGTCATGCGATTAACTTGGTGTTAACATCATTCGTCT
AACACTAGGGACGGTGGAAT

Downstream 100 bases:

>100_bases
TAATTGAGCACGGAGCGATCGAGTAAAGATCGTAGTGACGGAAAAAGCATGGTTAAAGGAAGAACATAAATGAACGCAAT
CACGAGAGTCATGGTCATTG

Product: Signal transduction histidine kinase regulating citrate/malate metabolism

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MKWSSISFRKRLLIIMTVTGLVELLILSGAGFYYIKQSQEQEMGLKALGVAEFLSESPLVTSIIQENGTLDPNGVPYVLS
EETQVKFRNLTQLIGAAFIVVGDDKGIRLIHPYDDRLGKPMRGGDNQKALVLGESYVSTAKGSLGFSVRGKSAVKDPQGT
VIGVVSVGYLLDSLQDRIEPYLAFLIVMALLVVAVNAVISSYVSRRFQRAILGFEPEEIGRLYVELDVTMSTVKEGILSI
DKSGILRSINKSACDILSIDRDKALNQQRLSDVLPGSDLEQLLSTGDTDHDVELYLNNKRIIANRSPIIVAGEVVGAVSS
FRLRDEINDLTDQLSQTKEYADLLRSQTHEHQNKLNTISGLIQMGELDSVQQLIGQETAHYQSLIEFLRETVKDPLIAGM
LLGKTERAREIGLELKVEEGSRLEALPAWLNAEDVVTILGNLIDNAFDATMMAIKQEGPIAPARRVIDVSISDFGNEIIV
EVEDKGCGLPKDLETKALTEKGVSSKAKQNRGVGLYLIKQLADRYQGQLEMVDNPDFGSRMTVYLPKEEQQ

Sequences:

>Translated_551_residues
MKWSSISFRKRLLIIMTVTGLVELLILSGAGFYYIKQSQEQEMGLKALGVAEFLSESPLVTSIIQENGTLDPNGVPYVLS
EETQVKFRNLTQLIGAAFIVVGDDKGIRLIHPYDDRLGKPMRGGDNQKALVLGESYVSTAKGSLGFSVRGKSAVKDPQGT
VIGVVSVGYLLDSLQDRIEPYLAFLIVMALLVVAVNAVISSYVSRRFQRAILGFEPEEIGRLYVELDVTMSTVKEGILSI
DKSGILRSINKSACDILSIDRDKALNQQRLSDVLPGSDLEQLLSTGDTDHDVELYLNNKRIIANRSPIIVAGEVVGAVSS
FRLRDEINDLTDQLSQTKEYADLLRSQTHEHQNKLNTISGLIQMGELDSVQQLIGQETAHYQSLIEFLRETVKDPLIAGM
LLGKTERAREIGLELKVEEGSRLEALPAWLNAEDVVTILGNLIDNAFDATMMAIKQEGPIAPARRVIDVSISDFGNEIIV
EVEDKGCGLPKDLETKALTEKGVSSKAKQNRGVGLYLIKQLADRYQGQLEMVDNPDFGSRMTVYLPKEEQQ
>Mature_551_residues
MKWSSISFRKRLLIIMTVTGLVELLILSGAGFYYIKQSQEQEMGLKALGVAEFLSESPLVTSIIQENGTLDPNGVPYVLS
EETQVKFRNLTQLIGAAFIVVGDDKGIRLIHPYDDRLGKPMRGGDNQKALVLGESYVSTAKGSLGFSVRGKSAVKDPQGT
VIGVVSVGYLLDSLQDRIEPYLAFLIVMALLVVAVNAVISSYVSRRFQRAILGFEPEEIGRLYVELDVTMSTVKEGILSI
DKSGILRSINKSACDILSIDRDKALNQQRLSDVLPGSDLEQLLSTGDTDHDVELYLNNKRIIANRSPIIVAGEVVGAVSS
FRLRDEINDLTDQLSQTKEYADLLRSQTHEHQNKLNTISGLIQMGELDSVQQLIGQETAHYQSLIEFLRETVKDPLIAGM
LLGKTERAREIGLELKVEEGSRLEALPAWLNAEDVVTILGNLIDNAFDATMMAIKQEGPIAPARRVIDVSISDFGNEIIV
EVEDKGCGLPKDLETKALTEKGVSSKAKQNRGVGLYLIKQLADRYQGQLEMVDNPDFGSRMTVYLPKEEQQ

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=458, Percent_Identity=36.0262008733624, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1790567, Length=550, Percent_Identity=28.7272727272727, Blast_Score=191, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60729; Mature: 60729

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWSSISFRKRLLIIMTVTGLVELLILSGAGFYYIKQSQEQEMGLKALGVAEFLSESPLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCHHHHHHHHHHCCCCHH
TSIIQENGTLDPNGVPYVLSEETQVKFRNLTQLIGAAFIVVGDDKGIRLIHPYDDRLGKP
HHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHCEEEEEEECCCCEEEECCCHHHCCCC
MRGGDNQKALVLGESYVSTAKGSLGFSVRGKSAVKDPQGTVIGVVSVGYLLDSLQDRIEP
CCCCCCCEEEEEECHHHHHCCCCCCEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
YLAFLIVMALLVVAVNAVISSYVSRRFQRAILGFEPEEIGRLYVELDVTMSTVKEGILSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEEEEECHHHHHHHHHHHC
DKSGILRSINKSACDILSIDRDKALNQQRLSDVLPGSDLEQLLSTGDTDHDVELYLNNKR
CHHHHHHHHCHHHHHHEECCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECCCE
IIANRSPIIVAGEVVGAVSSFRLRDEINDLTDQLSQTKEYADLLRSQTHEHQNKLNTISG
EEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIQMGELDSVQQLIGQETAHYQSLIEFLRETVKDPLIAGMLLGKTERAREIGLELKVEEG
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHCCCEEEECCC
SRLEALPAWLNAEDVVTILGNLIDNAFDATMMAIKQEGPIAPARRVIDVSISDFGNEIIV
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEHHHCCCEEEE
EVEDKGCGLPKDLETKALTEKGVSSKAKQNRGVGLYLIKQLADRYQGQLEMVDNPDFGSR
EECCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCE
MTVYLPKEEQQ
EEEEECCCCCC
>Mature Secondary Structure
MKWSSISFRKRLLIIMTVTGLVELLILSGAGFYYIKQSQEQEMGLKALGVAEFLSESPLV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCHHHHHHHHHHCCCCHH
TSIIQENGTLDPNGVPYVLSEETQVKFRNLTQLIGAAFIVVGDDKGIRLIHPYDDRLGKP
HHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHCEEEEEEECCCCEEEECCCHHHCCCC
MRGGDNQKALVLGESYVSTAKGSLGFSVRGKSAVKDPQGTVIGVVSVGYLLDSLQDRIEP
CCCCCCCEEEEEECHHHHHCCCCCCEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
YLAFLIVMALLVVAVNAVISSYVSRRFQRAILGFEPEEIGRLYVELDVTMSTVKEGILSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCEEEEEEECHHHHHHHHHHHC
DKSGILRSINKSACDILSIDRDKALNQQRLSDVLPGSDLEQLLSTGDTDHDVELYLNNKR
CHHHHHHHHCHHHHHHEECCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECCCE
IIANRSPIIVAGEVVGAVSSFRLRDEINDLTDQLSQTKEYADLLRSQTHEHQNKLNTISG
EEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIQMGELDSVQQLIGQETAHYQSLIEFLRETVKDPLIAGMLLGKTERAREIGLELKVEEG
HHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHCCCEEEECCC
SRLEALPAWLNAEDVVTILGNLIDNAFDATMMAIKQEGPIAPARRVIDVSISDFGNEIIV
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEHHHCCCEEEE
EVEDKGCGLPKDLETKALTEKGVSSKAKQNRGVGLYLIKQLADRYQGQLEMVDNPDFGSR
EECCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCE
MTVYLPKEEQQ
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]