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The map label for this gene is dpiA [H]

Identifier: 218709294

GI number: 218709294

Start: 1401653

End: 1402348

Strand: Direct

Name: dpiA [H]

Synonym: VS_1303

Alternate gene names: 218709294

Gene position: 1401653-1402348 (Clockwise)

Preceding gene: 218709293

Following gene: 218709295

Centisome position: 42.48

GC content: 46.12

Gene sequence:

>696_bases
ATGAACGCAATCACGAGAGTCATGGTCATTGAAGATGATATTGCGATTGCAGAGCTTCACCATCGTTATTTAGAACAGAT
GGGAGGCTTTGATGTGGTCGGGATTGCGACCACACAGTCTGAAGCATTAATGCAATTGGACATCTTAAAGCCTGATTTGG
TTTTATTGGATGTGTATCTACCGGACGGATGCGGACTTGATATTTTGAACCACGTTCGTGGTAGTAATCAGGGATGTGAT
GTGATTTTGATTACTGCTGCCCGAGATGTCGACACCTTGCAACAGGCGATGCGTGGTGGAGTCGTCGACTATTTACTGAA
ACCTGTGATGTTTCCTCGTTTGGAAGCGGCACTGAAAAAGTATCAGTCACAGCAGCAAGAGTTTGAAAGTGTGTCTGACT
TAAACCAAGGCTTGGTCGACAAGATGCTCCAAGCCAATGCCAAGGCAGATTCTCGAAAGGTTTCTACGTTACCTAAAGGG
ATCGATGGTGTAACGCTTGATAAAATTAGAGCTATTTTCCAACAAGCCGACATCGCTGATATTACTGCGGATGAGGCAGG
CGAACGCATTGGAGCAAGCCGAACGACAGCTCGACGCTATTTGGAATTCCTTATCACGACTGGCGAGTTAGTGGCTGACT
TAAATTACGGGTCAGTAGGTCGCCCAGAACGCTGCTACAACAAAGCTAGAAGATAG

Upstream 100 bases:

>100_bases
GGTGTATTTACCGAAAGAAGAACAACAATAATAATTGAGCACGGAGCGATCGAGTAAAGATCGTAGTGACGGAAAAAGCA
TGGTTAAAGGAAGAACATAA

Downstream 100 bases:

>100_bases
ATTTAGCGACAACAAGTCACAAAGATAAACGCGATGCTATCAAATAAATATAAAGCCAGTTGAGTAAGCTGGTTTTGGTT
TTTTCTCCCTTTCTTATTGC

Product: response regulator of citrate/malate metabolism

Products: NA

Alternate protein names: Destabilizer of plasmid inheritance [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MNAITRVMVIEDDIAIAELHHRYLEQMGGFDVVGIATTQSEALMQLDILKPDLVLLDVYLPDGCGLDILNHVRGSNQGCD
VILITAARDVDTLQQAMRGGVVDYLLKPVMFPRLEAALKKYQSQQQEFESVSDLNQGLVDKMLQANAKADSRKVSTLPKG
IDGVTLDKIRAIFQQADIADITADEAGERIGASRTTARRYLEFLITTGELVADLNYGSVGRPERCYNKARR

Sequences:

>Translated_231_residues
MNAITRVMVIEDDIAIAELHHRYLEQMGGFDVVGIATTQSEALMQLDILKPDLVLLDVYLPDGCGLDILNHVRGSNQGCD
VILITAARDVDTLQQAMRGGVVDYLLKPVMFPRLEAALKKYQSQQQEFESVSDLNQGLVDKMLQANAKADSRKVSTLPKG
IDGVTLDKIRAIFQQADIADITADEAGERIGASRTTARRYLEFLITTGELVADLNYGSVGRPERCYNKARR
>Mature_231_residues
MNAITRVMVIEDDIAIAELHHRYLEQMGGFDVVGIATTQSEALMQLDILKPDLVLLDVYLPDGCGLDILNHVRGSNQGCD
VILITAARDVDTLQQAMRGGVVDYLLKPVMFPRLEAALKKYQSQQQEFESVSDLNQGLVDKMLQANAKADSRKVSTLPKG
IDGVTLDKIRAIFQQADIADITADEAGERIGASRTTARRYLEFLITTGELVADLNYGSVGRPERCYNKARR

Specific function: Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox c

COG id: COG4565

COG function: function code KT; Response regulator of citrate/malate metabolism

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786838, Length=227, Percent_Identity=35.6828193832599, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1790566, Length=221, Percent_Identity=32.1266968325792, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25526; Mature: 25526

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAITRVMVIEDDIAIAELHHRYLEQMGGFDVVGIATTQSEALMQLDILKPDLVLLDVYL
CCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEC
PDGCGLDILNHVRGSNQGCDVILITAARDVDTLQQAMRGGVVDYLLKPVMFPRLEAALKK
CCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YQSQQQEFESVSDLNQGLVDKMLQANAKADSRKVSTLPKGIDGVTLDKIRAIFQQADIAD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCHHH
ITADEAGERIGASRTTARRYLEFLITTGELVADLNYGSVGRPERCYNKARR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MNAITRVMVIEDDIAIAELHHRYLEQMGGFDVVGIATTQSEALMQLDILKPDLVLLDVYL
CCCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCCEEEEEEEC
PDGCGLDILNHVRGSNQGCDVILITAARDVDTLQQAMRGGVVDYLLKPVMFPRLEAALKK
CCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YQSQQQEFESVSDLNQGLVDKMLQANAKADSRKVSTLPKGIDGVTLDKIRAIFQQADIAD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCHHH
ITADEAGERIGASRTTARRYLEFLITTGELVADLNYGSVGRPERCYNKARR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]