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The map label for this gene is pleD [H]

Identifier: 218709190

GI number: 218709190

Start: 1269453

End: 1270748

Strand: Direct

Name: pleD [H]

Synonym: VS_1197

Alternate gene names: 218709190

Gene position: 1269453-1270748 (Clockwise)

Preceding gene: 218709188

Following gene: 218709191

Centisome position: 38.48

GC content: 44.06

Gene sequence:

>1296_bases
GTGAAGTGGATTCATAAAATTGTTATCTTTCTAGTTATCGCAACACTTGCCATTGTGCAGTATTACCAGGTAAGTGGAAA
TCGTGTGATAACAACAATTACTCCTGAACAATATGAATTTATCGCGACCAGCGATCAAGTTGATAGAGGAGTCAGTACCT
CCCAATTGTCCTATGAAAATGGGCAGTATATTCTCGATTGTGAGCTTAAAAAATCCGAATACCCTTGGCCTTATTGCGGG
CTATCGATTCGTATTAACCCAGATATAACCACTGGCCTTGACCTTTCGCAGTACCACACCTTTAGAGTCAATATTGATTA
CCATGCGAAGGCTGATTCTAGTGGTCGTCTGAGAACTTACCTACGCAACTATAATCCTGCTTATTCGGTGCCTGATGACG
AATATACACATAAGTATAACGGGATGGAGTTTTCTCCAGGAATCGATGGTGGCGTGATAGAAATTCCGATCAAAAACTTG
CAAGTCATGACGTGGTGGTTAGCGGATAACGAGATTGCGTTAGAGCATTCAGCCCCTGAGTTTTCAAATGTGAACATGGT
TGAGTTTGCTACTGCTTCTGGTGCTAAATTAGGTCACCATCGGATAATTATTCGAAGTATTGAGTTTGAGGGGTCGTATA
TTTCGGCTGAAAGCCTGTTCCTGATCTTGCTGTTTATCTGGGTGGGTACAGGCACTGTGTTCCTGCTTTCAGAATTACAC
CGCTCAAGAAAACGTATCGCGATCGCTGAAAAAAGACACCATCACTTAAAAAACGTCAATCGGGCATTGAGAGAGCAAAA
CTTCGAGTTTTCTGAAAAGGCTCATCGTGATGAATTGACTGGGGTACTTAACCGACACGCGATTCGTGATTGGTTGAAAA
TGCAGTCGCAACAGGTGAAGCAAGGCCACGGCAAGTTAAGTATGCTCTACCTGGACATTGACTACTTTAAAAGTGTGAAT
GACAAATACGGACACCTGATGGGTGATCATATCTTGCGTGAGTTCAGTATGGTGGTTGGCAGCTCTATCAGTGCGACGGA
CAAACTTGTACGTTGGGGTGGTGAAGAGTTTATTGTTTTTTGCCCTGAAACCGAAGCTGGCGAAGCGCAGAGAAAGGCAG
AGAAAATCCGACTTTTGGTTAGCCAACATCTTTGGATTCATGGGGATTCTCTCACTTGCAGTATCGGCATTGCCGAGATG
AAACAAGAGCGGGTGACAGAGACCATTGCCCGCGCTGATGAAGCCTTATATCAAGCGAAGCATTCAGGGCGAAATCAGGT
GATCTTGAGTCACTAA

Upstream 100 bases:

>100_bases
TGTGATATAACGTTTTTAAGTCAATTGTTAGTATGCAATCTAATGATTAATTTATATCGTCAAATCCCAACTATAGGGAA
CAAAAGCCGGAAGCTTAGAT

Downstream 100 bases:

>100_bases
TCTATAATTTGGTTTGATAACTTAATAAGCCACACGTGCATATGTGACGTGTGGCGTTTTTATGGGCGCTATATGAGTTT
ACTCTCTAGTGCCTTTGTTG

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKWIHKIVIFLVIATLAIVQYYQVSGNRVITTITPEQYEFIATSDQVDRGVSTSQLSYENGQYILDCELKKSEYPWPYCG
LSIRINPDITTGLDLSQYHTFRVNIDYHAKADSSGRLRTYLRNYNPAYSVPDDEYTHKYNGMEFSPGIDGGVIEIPIKNL
QVMTWWLADNEIALEHSAPEFSNVNMVEFATASGAKLGHHRIIIRSIEFEGSYISAESLFLILLFIWVGTGTVFLLSELH
RSRKRIAIAEKRHHHLKNVNRALREQNFEFSEKAHRDELTGVLNRHAIRDWLKMQSQQVKQGHGKLSMLYLDIDYFKSVN
DKYGHLMGDHILREFSMVVGSSISATDKLVRWGGEEFIVFCPETEAGEAQRKAEKIRLLVSQHLWIHGDSLTCSIGIAEM
KQERVTETIARADEALYQAKHSGRNQVILSH

Sequences:

>Translated_431_residues
MKWIHKIVIFLVIATLAIVQYYQVSGNRVITTITPEQYEFIATSDQVDRGVSTSQLSYENGQYILDCELKKSEYPWPYCG
LSIRINPDITTGLDLSQYHTFRVNIDYHAKADSSGRLRTYLRNYNPAYSVPDDEYTHKYNGMEFSPGIDGGVIEIPIKNL
QVMTWWLADNEIALEHSAPEFSNVNMVEFATASGAKLGHHRIIIRSIEFEGSYISAESLFLILLFIWVGTGTVFLLSELH
RSRKRIAIAEKRHHHLKNVNRALREQNFEFSEKAHRDELTGVLNRHAIRDWLKMQSQQVKQGHGKLSMLYLDIDYFKSVN
DKYGHLMGDHILREFSMVVGSSISATDKLVRWGGEEFIVFCPETEAGEAQRKAEKIRLLVSQHLWIHGDSLTCSIGIAEM
KQERVTETIARADEALYQAKHSGRNQVILSH
>Mature_431_residues
MKWIHKIVIFLVIATLAIVQYYQVSGNRVITTITPEQYEFIATSDQVDRGVSTSQLSYENGQYILDCELKKSEYPWPYCG
LSIRINPDITTGLDLSQYHTFRVNIDYHAKADSSGRLRTYLRNYNPAYSVPDDEYTHKYNGMEFSPGIDGGVIEIPIKNL
QVMTWWLADNEIALEHSAPEFSNVNMVEFATASGAKLGHHRIIIRSIEFEGSYISAESLFLILLFIWVGTGTVFLLSELH
RSRKRIAIAEKRHHHLKNVNRALREQNFEFSEKAHRDELTGVLNRHAIRDWLKMQSQQVKQGHGKLSMLYLDIDYFKSVN
DKYGHLMGDHILREFSMVVGSSISATDKLVRWGGEEFIVFCPETEAGEAQRKAEKIRLLVSQHLWIHGDSLTCSIGIAEM
KQERVTETIARADEALYQAKHSGRNQVILSH

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=38.1818181818182, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=37.125748502994, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787541, Length=157, Percent_Identity=35.6687898089172, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=36.7088607594937, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=32.9192546583851, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=30.1775147928994, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787802, Length=210, Percent_Identity=30.952380952381, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=32.7272727272727, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=28.9017341040462, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=174, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788085, Length=174, Percent_Identity=28.735632183908, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=27.7456647398844, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 49396; Mature: 49396

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWIHKIVIFLVIATLAIVQYYQVSGNRVITTITPEQYEFIATSDQVDRGVSTSQLSYEN
CHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCEEEEECCHHHHCCCCCCEEEECC
GQYILDCELKKSEYPWPYCGLSIRINPDITTGLDLSQYHTFRVNIDYHAKADSSGRLRTY
CCEEEEEEECCCCCCCCEECEEEEECCCCCCCCCHHHEEEEEEEEEEEECCCCCCHHHHH
LRNYNPAYSVPDDEYTHKYNGMEFSPGIDGGVIEIPIKNLQVMTWWLADNEIALEHSAPE
HHCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCEEEEEEEEECCEEEEECCCCC
FSNVNMVEFATASGAKLGHHRIIIRSIEFEGSYISAESLFLILLFIWVGTGTVFLLSELH
CCCCCEEEEECCCCCCCCCEEEEEEEEEECCCEECHHHHHHHHHHHHHCCCHHHHHHHHH
RSRKRIAIAEKRHHHLKNVNRALREQNFEFSEKAHRDELTGVLNRHAIRDWLKMQSQQVK
HHHHHEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGHGKLSMLYLDIDYFKSVNDKYGHLMGDHILREFSMVVGSSISATDKLVRWGGEEFIVF
CCCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEE
CPETEAGEAQRKAEKIRLLVSQHLWIHGDSLTCSIGIAEMKQERVTETIARADEALYQAK
CCCCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
HSGRNQVILSH
HCCCCCEEEEC
>Mature Secondary Structure
MKWIHKIVIFLVIATLAIVQYYQVSGNRVITTITPEQYEFIATSDQVDRGVSTSQLSYEN
CHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCEEEEECCHHHHCCCCCCEEEECC
GQYILDCELKKSEYPWPYCGLSIRINPDITTGLDLSQYHTFRVNIDYHAKADSSGRLRTY
CCEEEEEEECCCCCCCCEECEEEEECCCCCCCCCHHHEEEEEEEEEEEECCCCCCHHHHH
LRNYNPAYSVPDDEYTHKYNGMEFSPGIDGGVIEIPIKNLQVMTWWLADNEIALEHSAPE
HHCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCEEEEEEEEECCEEEEECCCCC
FSNVNMVEFATASGAKLGHHRIIIRSIEFEGSYISAESLFLILLFIWVGTGTVFLLSELH
CCCCCEEEEECCCCCCCCCEEEEEEEEEECCCEECHHHHHHHHHHHHHCCCHHHHHHHHH
RSRKRIAIAEKRHHHLKNVNRALREQNFEFSEKAHRDELTGVLNRHAIRDWLKMQSQQVK
HHHHHEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGHGKLSMLYLDIDYFKSVNDKYGHLMGDHILREFSMVVGSSISATDKLVRWGGEEFIVF
CCCCCEEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEE
CPETEAGEAQRKAEKIRLLVSQHLWIHGDSLTCSIGIAEMKQERVTETIARADEALYQAK
CCCCCCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
HSGRNQVILSH
HCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA