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The map label for this gene is argT [H]

Identifier: 218709146

GI number: 218709146

Start: 1220659

End: 1221432

Strand: Direct

Name: argT [H]

Synonym: VS_1153

Alternate gene names: 218709146

Gene position: 1220659-1221432 (Clockwise)

Preceding gene: 218709145

Following gene: 218709148

Centisome position: 37.0

GC content: 44.7

Gene sequence:

>774_bases
ATGAAAAAGATAATACTAGGACTCGCTTGTGCGGCCGCTTTACTTGGTACAACGGTACAGGCGAAAGAAATCCGTTTGGC
GTCAGACTTTACGTATCCGCCATTCAACTATAAAAACAGTGACGGTGTGCCTGTCGGGTTTGATATTGAGATAGCTGACG
CTTTGTGTGAGCAAGCTAAACTCGATTGCACGTGGGTATCGCAAAGCTGGGATTCGTTGATTCCAAGTCTATTGGCGAGA
AAATCAGATGTGATCATGGCTTCGATGCGTATTACCGAAGAGCGTAAGCGCAAAATCTTGTTCACCGATAAGTACTACCA
AACACCAGCTGTGTTTGTTGCTGCTAAGAGCGCCGAGTTCAGCATTGATGAGGCCGGCTTAGCGGGTAAAACCATCGGTG
TTCAGCAAGGTACGATTCACGATCGCTACGTAACCGACAAGTTTGGTGATGTGGCAAACATTAAGCGTTATACCGGCCAA
GATGAAGTCTATCTGGATATGCAGAATGGGCGTCTAGATTTAACGTTTGGTAACTCTGATCAACTTTCATTGGCTTTCTT
AGATAAGAAAGAAGGTGAGGGCTTTGAATTCAAGGGTAAAGCGGTAACAGACAAAGCCTACATTGGTGAAGGTACGGCTT
TAGCACTAAGAAAGCAGGATTCAAAACTGGCGAAACAATTCAATGCCGCGATTGAAGAGATCAGAGCAAATGGCACCTAT
GATAAGATCGCAGCGAAGTACTTCACATTTGATATCTATGGCGCTGATTTGTAA

Upstream 100 bases:

>100_bases
CGCTACCAAACCGAATTAGAAAAGAATCAACAAGGCAAACAATCTCAGCATAAAGAAACTCAATCTCAACTCGATAAAGA
TCATATCTAGGATGGCAATG

Downstream 100 bases:

>100_bases
TTCGCCTAGCATCTGAAAGTAGAACACCAAAAACATCCGAAGGCTGTGAGGCTCTCGGATTTTTATTGGCTCCAAAAGAC
CATTGTTTAAGTGTTTAAGT

Product: lysine-arginine-ornithine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MKKIILGLACAAALLGTTVQAKEIRLASDFTYPPFNYKNSDGVPVGFDIEIADALCEQAKLDCTWVSQSWDSLIPSLLAR
KSDVIMASMRITEERKRKILFTDKYYQTPAVFVAAKSAEFSIDEAGLAGKTIGVQQGTIHDRYVTDKFGDVANIKRYTGQ
DEVYLDMQNGRLDLTFGNSDQLSLAFLDKKEGEGFEFKGKAVTDKAYIGEGTALALRKQDSKLAKQFNAAIEEIRANGTY
DKIAAKYFTFDIYGADL

Sequences:

>Translated_257_residues
MKKIILGLACAAALLGTTVQAKEIRLASDFTYPPFNYKNSDGVPVGFDIEIADALCEQAKLDCTWVSQSWDSLIPSLLAR
KSDVIMASMRITEERKRKILFTDKYYQTPAVFVAAKSAEFSIDEAGLAGKTIGVQQGTIHDRYVTDKFGDVANIKRYTGQ
DEVYLDMQNGRLDLTFGNSDQLSLAFLDKKEGEGFEFKGKAVTDKAYIGEGTALALRKQDSKLAKQFNAAIEEIRANGTY
DKIAAKYFTFDIYGADL
>Mature_257_residues
MKKIILGLACAAALLGTTVQAKEIRLASDFTYPPFNYKNSDGVPVGFDIEIADALCEQAKLDCTWVSQSWDSLIPSLLAR
KSDVIMASMRITEERKRKILFTDKYYQTPAVFVAAKSAEFSIDEAGLAGKTIGVQQGTIHDRYVTDKFGDVANIKRYTGQ
DEVYLDMQNGRLDLTFGNSDQLSLAFLDKKEGEGFEFKGKAVTDKAYIGEGTALALRKQDSKLAKQFNAAIEEIRANGTY
DKIAAKYFTFDIYGADL

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=262, Percent_Identity=39.3129770992366, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=42.1276595744681, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=37.0967741935484, Blast_Score=177, Evalue=4e-46,
Organism=Escherichia coli, GI1787085, Length=248, Percent_Identity=35.4838709677419, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=30.0448430493274, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=27.3809523809524, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28443; Mature: 28443

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIILGLACAAALLGTTVQAKEIRLASDFTYPPFNYKNSDGVPVGFDIEIADALCEQAK
CCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHC
LDCTWVSQSWDSLIPSLLARKSDVIMASMRITEERKRKILFTDKYYQTPAVFVAAKSAEF
CCEEEEHHHHHHHHHHHHHCCCCEEEEEEHHCHHHCCEEEEECCCCCCCEEEEEECCCCC
SIDEAGLAGKTIGVQQGTIHDRYVTDKFGDVANIKRYTGQDEVYLDMQNGRLDLTFGNSD
CCCCCCCCCCEECCCCCCCCCCEECCCCCCHHHHHHHCCCCEEEEEECCCEEEEEECCCC
QLSLAFLDKKEGEGFEFKGKAVTDKAYIGEGTALALRKQDSKLAKQFNAAIEEIRANGTY
CEEEEEEECCCCCCEEEECCEECCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCH
DKIAAKYFTFDIYGADL
HHHEEEEEEEEEEECCC
>Mature Secondary Structure
MKKIILGLACAAALLGTTVQAKEIRLASDFTYPPFNYKNSDGVPVGFDIEIADALCEQAK
CCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHC
LDCTWVSQSWDSLIPSLLARKSDVIMASMRITEERKRKILFTDKYYQTPAVFVAAKSAEF
CCEEEEHHHHHHHHHHHHHCCCCEEEEEEHHCHHHCCEEEEECCCCCCCEEEEEECCCCC
SIDEAGLAGKTIGVQQGTIHDRYVTDKFGDVANIKRYTGQDEVYLDMQNGRLDLTFGNSD
CCCCCCCCCCEECCCCCCCCCCEECCCCCCHHHHHHHCCCCEEEEEECCCEEEEEECCCC
QLSLAFLDKKEGEGFEFKGKAVTDKAYIGEGTALALRKQDSKLAKQFNAAIEEIRANGTY
CEEEEEEECCCCCCEEEECCEECCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHCCCCH
DKIAAKYFTFDIYGADL
HHHEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]