Click here to switch to the map view.

The map label for this gene is ruvB

Identifier: 218709102

GI number: 218709102

Start: 1172419

End: 1173435

Strand: Direct

Name: ruvB

Synonym: VS_1108

Alternate gene names: 218709102

Gene position: 1172419-1173435 (Clockwise)

Preceding gene: 218709101

Following gene: 218709103

Centisome position: 35.54

GC content: 46.31

Gene sequence:

>1017_bases
ATGATTGAAGCCGATCGCCTTATTGCACCGGACAACCCAGTATTCAAAGATGAAGATGTCATTGATCGTGCGATACGCCC
TAAAGCACTGGCTGACTATCAAGGTCAGGATCACGTCCGTGGTCAGATGGAAATTTTCATCAAAGCAGCACAAATGCGTA
ATGAGGCGCTCGATCATTTGTTGATATTTGGTCCTCCAGGCTTGGGCAAAACCACATTGGCAAACATTGTTGCTAATGAA
ATGGATGTGAACATTCGTACCACTTCAGGACCTGTATTAGAAAAAGCAGGTGACTTGGCGGCATTGCTGACCAACCTTGA
AGAAAATGATGTTCTATTCATTGATGAGATCCACCGCCTTAGTCCTGTGGTTGAAGAGGTGTTGTACCCGGCAATGGAAG
ATTACCAATTAGATATCATGATTGGTGAAGGCCCAGCGGCACGTTCTATCAAGATCGACCTCCCTCCTTTCACTTTGATT
GGCGCAACCACTCGTGCTGGTTCACTGACATCGCCGTTGCGTGACCGTTTCGGTATTACTCAGCGTCTTGAGTACTATAA
AGTTGAAGATTTACAAAACATCGTTCAACGAAGTGCTGATTGTCTTGGTCTTTCTATGGAATCGCAAGGGGCGTTAGAAG
TTGCTCGTCGAGCTCGTGGTACGCCACGTATTGCTAACCGTTTGCTGCGCCGTGTTCGTGACTATGCGGAAGTGAAAGGC
GATGGACATATTTGCCCAGAGGTTGCTGATAAAGCGCTAAACATGTTGGATGTCGATGCTAAAGGTTTCGACTACATGGA
TAGGAAGCTTTTGCTTGCGATTATGGAGAAGTTTGGTGGTGGCCCGGTTGGTATAGACAACATGGCTGCGGCGATTGGTG
AAGAGAGAGATACCATAGAAGATGTGTTAGAACCTTACTTGATTCAGCAAGGTTATCTACAAAGAACGCCAAGAGGTCGA
ATCGCGACCGACAGGGCGTATTTACACTTCGGCATCGATAAACCATCTAATCGTTAA

Upstream 100 bases:

>100_bases
CCGATATACTTGTTCTACAAAATAGACTCATTCACTTTGCCTTTACACAGAATCAAGAAGCGCAGAGTCAACAAGAGCAT
AGTTAGGAATAGCAGAATTT

Downstream 100 bases:

>100_bases
TTTATTCATTTTTAAAGATCCCAGTTGCATAAGCGCTGGGATTTTTTGCTTTCTATTGAATAACAACTCTTTGAGCGAGT
CGATAAAAAAGCTGAATAGC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MIEADRLIAPDNPVFKDEDVIDRAIRPKALADYQGQDHVRGQMEIFIKAAQMRNEALDHLLIFGPPGLGKTTLANIVANE
MDVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGITQRLEYYKVEDLQNIVQRSADCLGLSMESQGALEVARRARGTPRIANRLLRRVRDYAEVKG
DGHICPEVADKALNMLDVDAKGFDYMDRKLLLAIMEKFGGGPVGIDNMAAAIGEERDTIEDVLEPYLIQQGYLQRTPRGR
IATDRAYLHFGIDKPSNR

Sequences:

>Translated_338_residues
MIEADRLIAPDNPVFKDEDVIDRAIRPKALADYQGQDHVRGQMEIFIKAAQMRNEALDHLLIFGPPGLGKTTLANIVANE
MDVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGITQRLEYYKVEDLQNIVQRSADCLGLSMESQGALEVARRARGTPRIANRLLRRVRDYAEVKG
DGHICPEVADKALNMLDVDAKGFDYMDRKLLLAIMEKFGGGPVGIDNMAAAIGEERDTIEDVLEPYLIQQGYLQRTPRGR
IATDRAYLHFGIDKPSNR
>Mature_338_residues
MIEADRLIAPDNPVFKDEDVIDRAIRPKALADYQGQDHVRGQMEIFIKAAQMRNEALDHLLIFGPPGLGKTTLANIVANE
MDVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLI
GATTRAGSLTSPLRDRFGITQRLEYYKVEDLQNIVQRSADCLGLSMESQGALEVARRARGTPRIANRLLRRVRDYAEVKG
DGHICPEVADKALNMLDVDAKGFDYMDRKLLLAIMEKFGGGPVGIDNMAAAIGEERDTIEDVLEPYLIQQGYLQRTPRGR
IATDRAYLHFGIDKPSNR

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=335, Percent_Identity=80.5970149253731, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1787119, Length=181, Percent_Identity=27.6243093922652, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_VIBSL (B7VMI4)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416723.1
- ProteinModelPortal:   B7VMI4
- SMR:   B7VMI4
- GeneID:   7160700
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_1108
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37597; Mature: 37597

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEADRLIAPDNPVFKDEDVIDRAIRPKALADYQGQDHVRGQMEIFIKAAQMRNEALDHL
CCCCCCEECCCCCCCCCHHHHHHHCCCCHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHEE
LIFGPPGLGKTTLANIVANEMDVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
SPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIT
HHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHCHH
QRLEYYKVEDLQNIVQRSADCLGLSMESQGALEVARRARGTPRIANRLLRRVRDYAEVKG
HHHHHHHHHHHHHHHHHCHHHEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DGHICPEVADKALNMLDVDAKGFDYMDRKLLLAIMEKFGGGPVGIDNMAAAIGEERDTIE
CCCCCHHHHHHHHHEHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
DVLEPYLIQQGYLQRTPRGRIATDRAYLHFGIDKPSNR
HHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCCCC
>Mature Secondary Structure
MIEADRLIAPDNPVFKDEDVIDRAIRPKALADYQGQDHVRGQMEIFIKAAQMRNEALDHL
CCCCCCEECCCCCCCCCHHHHHHHCCCCHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHEE
LIFGPPGLGKTTLANIVANEMDVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRL
EEECCCCCCHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHH
SPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIT
HHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEECCCEEEEECCCCCCCCCHHHHHHHCHH
QRLEYYKVEDLQNIVQRSADCLGLSMESQGALEVARRARGTPRIANRLLRRVRDYAEVKG
HHHHHHHHHHHHHHHHHCHHHEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DGHICPEVADKALNMLDVDAKGFDYMDRKLLLAIMEKFGGGPVGIDNMAAAIGEERDTIE
CCCCCHHHHHHHHHEHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHH
DVLEPYLIQQGYLQRTPRGRIATDRAYLHFGIDKPSNR
HHHHHHHHHHHHHHCCCCCCEEECCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA