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The map label for this gene is ruvA

Identifier: 218709101

GI number: 218709101

Start: 1171704

End: 1172315

Strand: Direct

Name: ruvA

Synonym: VS_1107

Alternate gene names: 218709101

Gene position: 1171704-1172315 (Clockwise)

Preceding gene: 218709100

Following gene: 218709102

Centisome position: 35.51

GC content: 46.57

Gene sequence:

>612_bases
GTGATCGGACGTCTCCGCGGTACATTAATAGAAAAACAGCCACCAGAGTTATTGATTGAAGTCAGTGGTGTTGGTTATGA
AGTTCAAATGCCAATGAGCTGTTTTTATGAGTTACCAAACGTAGGCGAAGAGGCAATTATCTACACTCATTTTGTGGTAC
GTGAAGATGCTCAGCTACTTTATGGTTTTAACACGGTTAAAGAGCGTGCCTTGTTCCGTGAAGTGATTAAAGCTAATGGT
GTCGGCCCTAAACTTGGTCTTGGGATTCTTTCAGGCATGACCGCTAGCCAATTCGTTCAGAGCGTTGAACGAGAAGATAT
CTCTACGCTGGTTAAACTGCCGGGTGTTGGTAAGAAAACGGCAGAACGCCTTGTTGTTGAAATGAAAGACCGCCTGAAAG
GGTGGGGCGCAGGTGATCTGTTTACACCTGCAACAGATGCAGCGCCAATGGACTCTATGCCAACGGTGCACGACGCGGAA
GAAGAAGCGGTAAGTGCGCTACTTGCATTAGGCTACAAGCCGACTCAAGCTTCTAAGGTGGTTTCTCAAGTAGCTAAAGA
TGGCATGACCAGCGAACAGTTGATTCGCGAAGCTCTGAAATCGATGGTTTAA

Upstream 100 bases:

>100_bases
AAGCCTAAATTATTCACTTCAAAAAACACAAAGAACTGGATAAGTATCCAGTTCTTTTATATTCTGTCCCTCAAATTAAA
TTCCATCAAGAGAGTGAATT

Downstream 100 bases:

>100_bases
TACCCGATATACTTGTTCTACAAAATAGACTCATTCACTTTGCCTTTACACAGAATCAAGAAGCGCAGAGTCAACAAGAG
CATAGTTAGGAATAGCAGAA

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MIGRLRGTLIEKQPPELLIEVSGVGYEVQMPMSCFYELPNVGEEAIIYTHFVVREDAQLLYGFNTVKERALFREVIKANG
VGPKLGLGILSGMTASQFVQSVEREDISTLVKLPGVGKKTAERLVVEMKDRLKGWGAGDLFTPATDAAPMDSMPTVHDAE
EEAVSALLALGYKPTQASKVVSQVAKDGMTSEQLIREALKSMV

Sequences:

>Translated_203_residues
MIGRLRGTLIEKQPPELLIEVSGVGYEVQMPMSCFYELPNVGEEAIIYTHFVVREDAQLLYGFNTVKERALFREVIKANG
VGPKLGLGILSGMTASQFVQSVEREDISTLVKLPGVGKKTAERLVVEMKDRLKGWGAGDLFTPATDAAPMDSMPTVHDAE
EEAVSALLALGYKPTQASKVVSQVAKDGMTSEQLIREALKSMV
>Mature_203_residues
MIGRLRGTLIEKQPPELLIEVSGVGYEVQMPMSCFYELPNVGEEAIIYTHFVVREDAQLLYGFNTVKERALFREVIKANG
VGPKLGLGILSGMTASQFVQSVEREDISTLVKLPGVGKKTAERLVVEMKDRLKGWGAGDLFTPATDAAPMDSMPTVHDAE
EEAVSALLALGYKPTQASKVVSQVAKDGMTSEQLIREALKSMV

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=202, Percent_Identity=68.3168316831683, Blast_Score=286, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_VIBSL (B7VMI3)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416722.1
- ProteinModelPortal:   B7VMI3
- SMR:   B7VMI3
- GeneID:   7160699
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_1107
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22115; Mature: 22115

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRLRGTLIEKQPPELLIEVSGVGYEVQMPMSCFYELPNVGEEAIIYTHFVVREDAQLL
CCCCCCCHHHCCCCCCEEEEECCCCEEEECCHHHHHHCCCCCCCCEEHHHHHHHCCHHHH
YGFNTVKERALFREVIKANGVGPKLGLGILSGMTASQFVQSVEREDISTLVKLPGVGKKT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
AERLVVEMKDRLKGWGAGDLFTPATDAAPMDSMPTVHDAEEEAVSALLALGYKPTQASKV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
VSQVAKDGMTSEQLIREALKSMV
HHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIGRLRGTLIEKQPPELLIEVSGVGYEVQMPMSCFYELPNVGEEAIIYTHFVVREDAQLL
CCCCCCCHHHCCCCCCEEEEECCCCEEEECCHHHHHHCCCCCCCCEEHHHHHHHCCHHHH
YGFNTVKERALFREVIKANGVGPKLGLGILSGMTASQFVQSVEREDISTLVKLPGVGKKT
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
AERLVVEMKDRLKGWGAGDLFTPATDAAPMDSMPTVHDAEEEAVSALLALGYKPTQASKV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
VSQVAKDGMTSEQLIREALKSMV
HHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA