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The map label for this gene is rluF [H]

Identifier: 218708762

GI number: 218708762

Start: 780300

End: 781439

Strand: Reverse

Name: rluF [H]

Synonym: VS_0759

Alternate gene names: 218708762

Gene position: 781439-780300 (Counterclockwise)

Preceding gene: 218708763

Following gene: 218708753

Centisome position: 23.68

GC content: 45.09

Gene sequence:

>1140_bases
ATGTCACAAGAATCCCAAGCTAAACGCCTTAATAAATACATCAGTGAAACTGGTTTTTGCTCACGCCGCGAAGCCGACAA
ACTCATTGATGCTGGCCGAGTTACTATTAACGGTAAGATCCCTGAAATGGGCACTAAAGTCTTGCCTGGTGATGATGTTG
AGATCGACAATAAACCTGTTCGTTCAAGAGAAAAGCCGATCTACATTGCTCTTAATAAACCAACAGGTATCACCTGTACT
ACTGAGCGTGATATTCCAGGCAACATCGTCGACTTTATAGGTCACCACAAACGTATATTCCCTATTGGTCGTCTAGATAA
GCCTTCTGATGGTCTTATCTTCTTGACCAACGACGGCGATATCGTAAACAAAATCCTACGTGCAGGTAACAACCACGAAA
AAGAATACGTGGTTCGTGTAGATAAACCTATTACGACTGAGTTCTTGAAGCAAATGGGCGCTGGCGTTCATATTCTCGAC
ACTGTTACCTTACCATGTAAGGTAGAGAAAGAGACCAAGTTCTCGTTTCGCATCACATTAACGCAAGGCCTTAACCGTCA
GATTCGCCGTATGTGTGAAGCTCTCGGATATGAAGTATTTAAGCTTCGCCGTGTTCGTATTATGAACATCTCATTAGACG
GCATTCCTAACGGAAAATGGCGCTACCTGAGCGACGAAGAGATTACTGAAATCTTGGCAATGTGTGAAGGCTCAGTAAGT
ACTGAAGATGCGTCAAAAATGAACGCGAAAGGTCAACGCATTCGTAAAGCGACTGACGCGAAACTTTTTGATAGCCGTGA
AGAGAACCAAACTTCAACTGCGCGCCGTAATCAAAACGAGAACCGTGCTCGCACTTACCGCGGTAACAATGCGGATGAAT
TCCGTCATGCGCCTAACTCAAAGCGTGGCCGTAACTCATCGAACGGTGAAAGCAGTGGCGGTGGCAATACCGAGAACTGG
AAATCAAACTCTCGTTCAGAGCGTTCTAATTCAGATAAAAACCGTTCGGATCGCAATCGCACAGATCGCGACAATAACGA
GCGTAGAAGCGGTAAACCGACAAACCGCAGTCAAGATTCAAACAGACCGAACAAGTCTGCACCGAAACGTGTTGGTGGTA
CTTTAGGTTTGAAAAAGTAG

Upstream 100 bases:

>100_bases
CTAAAATTGTCCTCAGTTAACGAATCCCAACTCAAAGCAACTATTCAGAGGTTGCTTTTTGTTTATCTATATTTCCGTTG
TGAAACGCTAAGAGAATATC

Downstream 100 bases:

>100_bases
TCAGACCCCTTTCAAACGGGACTGTCGATAAAGAAACGCCCGCTAGATTAGTCTAGCGGGCGTTTTATTTATGATCATGT
ACGTTTATCGTTATGGAAAG

Product: ribosomal large subunit pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSQESQAKRLNKYISETGFCSRREADKLIDAGRVTINGKIPEMGTKVLPGDDVEIDNKPVRSREKPIYIALNKPTGITCT
TERDIPGNIVDFIGHHKRIFPIGRLDKPSDGLIFLTNDGDIVNKILRAGNNHEKEYVVRVDKPITTEFLKQMGAGVHILD
TVTLPCKVEKETKFSFRITLTQGLNRQIRRMCEALGYEVFKLRRVRIMNISLDGIPNGKWRYLSDEEITEILAMCEGSVS
TEDASKMNAKGQRIRKATDAKLFDSREENQTSTARRNQNENRARTYRGNNADEFRHAPNSKRGRNSSNGESSGGGNTENW
KSNSRSERSNSDKNRSDRNRTDRDNNERRSGKPTNRSQDSNRPNKSAPKRVGGTLGLKK

Sequences:

>Translated_379_residues
MSQESQAKRLNKYISETGFCSRREADKLIDAGRVTINGKIPEMGTKVLPGDDVEIDNKPVRSREKPIYIALNKPTGITCT
TERDIPGNIVDFIGHHKRIFPIGRLDKPSDGLIFLTNDGDIVNKILRAGNNHEKEYVVRVDKPITTEFLKQMGAGVHILD
TVTLPCKVEKETKFSFRITLTQGLNRQIRRMCEALGYEVFKLRRVRIMNISLDGIPNGKWRYLSDEEITEILAMCEGSVS
TEDASKMNAKGQRIRKATDAKLFDSREENQTSTARRNQNENRARTYRGNNADEFRHAPNSKRGRNSSNGESSGGGNTENW
KSNSRSERSNSDKNRSDRNRTDRDNNERRSGKPTNRSQDSNRPNKSAPKRVGGTLGLKK
>Mature_378_residues
SQESQAKRLNKYISETGFCSRREADKLIDAGRVTINGKIPEMGTKVLPGDDVEIDNKPVRSREKPIYIALNKPTGITCTT
ERDIPGNIVDFIGHHKRIFPIGRLDKPSDGLIFLTNDGDIVNKILRAGNNHEKEYVVRVDKPITTEFLKQMGAGVHILDT
VTLPCKVEKETKFSFRITLTQGLNRQIRRMCEALGYEVFKLRRVRIMNISLDGIPNGKWRYLSDEEITEILAMCEGSVST
EDASKMNAKGQRIRKATDAKLFDSREENQTSTARRNQNENRARTYRGNNADEFRHAPNSKRGRNSSNGESSGGGNTENWK
SNSRSERSNSDKNRSDRNRTDRDNNERRSGKPTNRSQDSNRPNKSAPKRVGGTLGLKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=234, Percent_Identity=62.8205128205128, Blast_Score=277, Evalue=6e-76,
Organism=Escherichia coli, GI1787524, Length=295, Percent_Identity=31.864406779661, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=32.1739130434783, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI87081838, Length=146, Percent_Identity=32.8767123287671, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 42840; Mature: 42708

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQESQAKRLNKYISETGFCSRREADKLIDAGRVTINGKIPEMGTKVLPGDDVEIDNKPV
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
RSREKPIYIALNKPTGITCTTERDIPGNIVDFIGHHKRIFPIGRLDKPSDGLIFLTNDGD
CCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCH
IVNKILRAGNNHEKEYVVRVDKPITTEFLKQMGAGVHILDTVTLPCKVEKETKFSFRITL
HHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEEECCEEECCCCCEEEEEEE
TQGLNRQIRRMCEALGYEVFKLRRVRIMNISLDGIPNGKWRYLSDEEITEILAMCEGSVS
HHHHHHHHHHHHHHHCHHHHHHEEEEEEEEEECCCCCCCEEEECHHHHHHHHHHHCCCCC
TEDASKMNAKGQRIRKATDAKLFDSREENQTSTARRNQNENRARTYRGNNADEFRHAPNS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHCCCCCHHHHHCCCCC
KRGRNSSNGESSGGGNTENWKSNSRSERSNSDKNRSDRNRTDRDNNERRSGKPTNRSQDS
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
NRPNKSAPKRVGGTLGLKK
CCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
SQESQAKRLNKYISETGFCSRREADKLIDAGRVTINGKIPEMGTKVLPGDDVEIDNKPV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
RSREKPIYIALNKPTGITCTTERDIPGNIVDFIGHHKRIFPIGRLDKPSDGLIFLTNDGD
CCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCH
IVNKILRAGNNHEKEYVVRVDKPITTEFLKQMGAGVHILDTVTLPCKVEKETKFSFRITL
HHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEEECCEEECCCCCEEEEEEE
TQGLNRQIRRMCEALGYEVFKLRRVRIMNISLDGIPNGKWRYLSDEEITEILAMCEGSVS
HHHHHHHHHHHHHHHCHHHHHHEEEEEEEEEECCCCCCCEEEECHHHHHHHHHHHCCCCC
TEDASKMNAKGQRIRKATDAKLFDSREENQTSTARRNQNENRARTYRGNNADEFRHAPNS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHCCCCCHHHHHCCCCC
KRGRNSSNGESSGGGNTENWKSNSRSERSNSDKNRSDRNRTDRDNNERRSGKPTNRSQDS
CCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
NRPNKSAPKRVGGTLGLKK
CCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]