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The map label for this gene is lolB

Identifier: 218708753

GI number: 218708753

Start: 771028

End: 771675

Strand: Reverse

Name: lolB

Synonym: VS_0750

Alternate gene names: 218708753

Gene position: 771675-771028 (Counterclockwise)

Preceding gene: 218708762

Following gene: 218708752

Centisome position: 23.39

GC content: 43.06

Gene sequence:

>648_bases
ATGAGCAAGCTTCGAAAAATCACGTCTCTTATTTTTTTGACCATCATTATGGTGGGTTGCTCGTCTATCCCAGAACAACC
GACCAGCGTTGAGTGGCAAAGTCACCAAAACAGGCTTTTACAGATAGAAAACTATCAAGCTTCAGGCAAGCTCGCTTACA
TTTCTCCAGAGCAACGCCAAAGCCTAAACTTCATATGGAAGCACTCCCCAAATCAAAGCCAATTAAGACTGACCACTTTC
CTTGGTCAAACCGCCTTGAACCTTACAATTGACAGTTTGGGTGCAAAAGTCGTGACTTACGATGATCAAGTATTCACCCA
TGCAAGTGCATCTGTATTAGTAGAGCAACTTACTGGATTGCAGATCCCCATTGATCACCTACCACAATGGTTCCTTGGCA
TTCCCGACCAAGCAGATAGCTACCAACTCAACAGCACCAACACACTTGAATCTCTGTCTAAACAAGTCAGCAGTCAATTG
TGGACCCTGAATTTTGCTAACTATCGCAATACAGAGATGCAGAGTAAGCAACTATCGGATAAAGACAACGTAAAGGTAGA
GACAATCCCCCTCCCTACTCGATTGTCATTCAAGCAAGACGAGAACAAAATCAACATTGTAGTTTCGAAGTGGACACTGA
AAAAATGA

Upstream 100 bases:

>100_bases
TCTATTTAAAGAGCAAGCGGGATTTGTTTTCCTGAGTTCGTGAATTCAATGCTATAGTTGAGACGTTTTTTAGATAAATT
TGAACAAGTTGTGAGCAAAT

Downstream 100 bases:

>100_bases
TAACAACGCCAACTCACTGGCCTTCGCCAGCTAAGCTGAATTTATTTCTCTACATCACTGGCCGACGCGACAATGGCTAT
CACGAACTTCAGACCCTATT

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSKLRKITSLIFLTIIMVGCSSIPEQPTSVEWQSHQNRLLQIENYQASGKLAYISPEQRQSLNFIWKHSPNQSQLRLTTF
LGQTALNLTIDSLGAKVVTYDDQVFTHASASVLVEQLTGLQIPIDHLPQWFLGIPDQADSYQLNSTNTLESLSKQVSSQL
WTLNFANYRNTEMQSKQLSDKDNVKVETIPLPTRLSFKQDENKINIVVSKWTLKK

Sequences:

>Translated_215_residues
MSKLRKITSLIFLTIIMVGCSSIPEQPTSVEWQSHQNRLLQIENYQASGKLAYISPEQRQSLNFIWKHSPNQSQLRLTTF
LGQTALNLTIDSLGAKVVTYDDQVFTHASASVLVEQLTGLQIPIDHLPQWFLGIPDQADSYQLNSTNTLESLSKQVSSQL
WTLNFANYRNTEMQSKQLSDKDNVKVETIPLPTRLSFKQDENKINIVVSKWTLKK
>Mature_214_residues
SKLRKITSLIFLTIIMVGCSSIPEQPTSVEWQSHQNRLLQIENYQASGKLAYISPEQRQSLNFIWKHSPNQSQLRLTTFL
GQTALNLTIDSLGAKVVTYDDQVFTHASASVLVEQLTGLQIPIDHLPQWFLGIPDQADSYQLNSTNTLESLSKQVSSQLW
TLNFANYRNTEMQSKQLSDKDNVKVETIPLPTRLSFKQDENKINIVVSKWTLKK

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family

Homologues:

Organism=Escherichia coli, GI1787460, Length=220, Percent_Identity=25.4545454545455, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LOLB_VIBSL (B7VKH1)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416374.1
- GeneID:   7160351
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0750
- HOGENOM:   HBG644390
- OMA:   FSSRFEW
- ProtClustDB:   PRK00022
- HAMAP:   MF_00233
- InterPro:   IPR004565
- TIGRFAMs:   TIGR00548

Pfam domain/function: PF03550 LolB

EC number: NA

Molecular weight: Translated: 24480; Mature: 24349

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLRKITSLIFLTIIMVGCSSIPEQPTSVEWQSHQNRLLQIENYQASGKLAYISPEQRQ
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCEEEEECCCCCCEEEEECCHHHC
SLNFIWKHSPNQSQLRLTTFLGQTALNLTIDSLGAKVVTYDDQVFTHASASVLVEQLTGL
CCEEEEECCCCCCCEEEEEHHCCHHEEEEEHHCCCEEEEECCHHHHHHHHHHHHHHHCCC
QIPIDHLPQWFLGIPDQADSYQLNSTNTLESLSKQVSSQLWTLNFANYRNTEMQSKQLSD
CCCHHHCCHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHCCC
KDNVKVETIPLPTRLSFKQDENKINIVVSKWTLKK
CCCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCC
>Mature Secondary Structure 
SKLRKITSLIFLTIIMVGCSSIPEQPTSVEWQSHQNRLLQIENYQASGKLAYISPEQRQ
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCEEEEECCCCCCEEEEECCHHHC
SLNFIWKHSPNQSQLRLTTFLGQTALNLTIDSLGAKVVTYDDQVFTHASASVLVEQLTGL
CCEEEEECCCCCCCEEEEEHHCCHHEEEEEHHCCCEEEEECCHHHHHHHHHHHHHHHCCC
QIPIDHLPQWFLGIPDQADSYQLNSTNTLESLSKQVSSQLWTLNFANYRNTEMQSKQLSD
CCCHHHCCHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHCCC
KDNVKVETIPLPTRLSFKQDENKINIVVSKWTLKK
CCCCEEEEECCCCCCCCCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA