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The map label for this gene is ipk

Identifier: 218708752

GI number: 218708752

Start: 770156

End: 771046

Strand: Reverse

Name: ipk

Synonym: VS_0749

Alternate gene names: 218708752

Gene position: 771046-770156 (Counterclockwise)

Preceding gene: 218708753

Following gene: 218708751

Centisome position: 23.37

GC content: 46.13

Gene sequence:

>891_bases
GTGGACACTGAAAAAATGATAACAACGCCAACTCACTGGCCTTCGCCAGCTAAGCTGAATTTATTTCTCTACATCACTGG
CCGACGCGACAATGGCTATCACGAACTTCAGACCCTATTTCAGTTTGTCGACTTTGGCGATGAACTTACGGTTACCGCAA
ACCGAGAAACAAGCTCGATAACAATCACACCTGAAATTCCAGGTGTTGCGACAGAAGATAACCTGATTTGGAAAGCCGCA
ACTGCCCTTCAACAACATACATCGACGACTTTCGGTGCGGATATTCAGTTAAAGAAAGTGCTTCCTATGGGAGGTGGCAT
TGGCGGAGGTTCTTCAAATGCCGCAACCGTGTTAGTCGCTCTCAACCATTTATGGCAACTCAACCTGTCTGATGACCAGT
TAGCAGAGATTGGCTTAAAGCTTGGTGCAGACGTTCCGGTCTTCGTTAGAGGCCACGCCGCCTTTGCTGAAGGTGTTGGT
GAACAACTGCAACCCGCTAATCCGGATGAAAAATGGTATCTCGTTGTTAAACCTCAAGTGAGCATAGCAACTGTAGACAT
ATTCACACATTCAGAATTAACTCGAAACACGCCGAAGCGAGCACTATCAACGCTTCTAGAGCAAGAATACGTAAACGATT
GCGAAAAAATTGTGCGAATGCTGTACCCAGAGGTTGATAAGCAACTTTCATGGCTGCTACAATACGCGCCGTCGAGATTG
ACTGGTACAGGTTCGTGCGTTTTTGCTGAATTTAGCAGCAAAAAAGAAGCCGAACTGGTGCTAGAGCAACTACCTGACAC
AGTTTCCGCGTTTGTCGCGAAAGGACGAAACATTTCGCCTTTGAAAGAAACTTTGGCTGAATACCAATCAGCCCACCCAC
AATCTATTTAA

Upstream 100 bases:

>100_bases
AACTATCGGATAAAGACAACGTAAAGGTAGAGACAATCCCCCTCCCTACTCGATTGTCATTCAAGCAAGACGAGAACAAA
ATCAACATTGTAGTTTCGAA

Downstream 100 bases:

>100_bases
AACTGGACGCAACCCGAGGTTTCCACCGTGCCTGATATGAAGCTATTTGCTGGTAACGCAACACCTGAACTAGCCCAACG
TATTGCTGATCGTCTATACA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDTEKMITTPTHWPSPAKLNLFLYITGRRDNGYHELQTLFQFVDFGDELTVTANRETSSITITPEIPGVATEDNLIWKAA
TALQQHTSTTFGADIQLKKVLPMGGGIGGGSSNAATVLVALNHLWQLNLSDDQLAEIGLKLGADVPVFVRGHAAFAEGVG
EQLQPANPDEKWYLVVKPQVSIATVDIFTHSELTRNTPKRALSTLLEQEYVNDCEKIVRMLYPEVDKQLSWLLQYAPSRL
TGTGSCVFAEFSSKKEAELVLEQLPDTVSAFVAKGRNISPLKETLAEYQSAHPQSI

Sequences:

>Translated_296_residues
MDTEKMITTPTHWPSPAKLNLFLYITGRRDNGYHELQTLFQFVDFGDELTVTANRETSSITITPEIPGVATEDNLIWKAA
TALQQHTSTTFGADIQLKKVLPMGGGIGGGSSNAATVLVALNHLWQLNLSDDQLAEIGLKLGADVPVFVRGHAAFAEGVG
EQLQPANPDEKWYLVVKPQVSIATVDIFTHSELTRNTPKRALSTLLEQEYVNDCEKIVRMLYPEVDKQLSWLLQYAPSRL
TGTGSCVFAEFSSKKEAELVLEQLPDTVSAFVAKGRNISPLKETLAEYQSAHPQSI
>Mature_296_residues
MDTEKMITTPTHWPSPAKLNLFLYITGRRDNGYHELQTLFQFVDFGDELTVTANRETSSITITPEIPGVATEDNLIWKAA
TALQQHTSTTFGADIQLKKVLPMGGGIGGGSSNAATVLVALNHLWQLNLSDDQLAEIGLKLGADVPVFVRGHAAFAEGVG
EQLQPANPDEKWYLVVKPQVSIATVDIFTHSELTRNTPKRALSTLLEQEYVNDCEKIVRMLYPEVDKQLSWLLQYAPSRL
TGTGSCVFAEFSSKKEAELVLEQLPDTVSAFVAKGRNISPLKETLAEYQSAHPQSI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=281, Percent_Identity=62.2775800711744, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_VIBSL (B7VKH0)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416373.1
- ProteinModelPortal:   B7VKH0
- GeneID:   7160350
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0749
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- ProtClustDB:   PRK00343
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32569; Mature: 32569

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: ACT_SITE 18-18 ACT_SITE 144-144

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTEKMITTPTHWPSPAKLNLFLYITGRRDNGYHELQTLFQFVDFGDELTVTANRETSSI
CCCCCEEECCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEE
TITPEIPGVATEDNLIWKAATALQQHTSTTFGADIQLKKVLPMGGGIGGGSSNAATVLVA
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHEE
LNHLWQLNLSDDQLAEIGLKLGADVPVFVRGHAAFAEGVGEQLQPANPDEKWYLVVKPQV
EHHHEEECCCHHHHHHHHHHCCCCCCEEEECCHHHHHHCCHHCCCCCCCCCEEEEECCCC
SIATVDIFTHSELTRNTPKRALSTLLEQEYVNDCEKIVRMLYPEVDKQLSWLLQYAPSRL
EEEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TGTGSCVFAEFSSKKEAELVLEQLPDTVSAFVAKGRNISPLKETLAEYQSAHPQSI
CCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDTEKMITTPTHWPSPAKLNLFLYITGRRDNGYHELQTLFQFVDFGDELTVTANRETSSI
CCCCCEEECCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEE
TITPEIPGVATEDNLIWKAATALQQHTSTTFGADIQLKKVLPMGGGIGGGSSNAATVLVA
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHEE
LNHLWQLNLSDDQLAEIGLKLGADVPVFVRGHAAFAEGVGEQLQPANPDEKWYLVVKPQV
EHHHEEECCCHHHHHHHHHHCCCCCCEEEECCHHHHHHCCHHCCCCCCCCCEEEEECCCC
SIATVDIFTHSELTRNTPKRALSTLLEQEYVNDCEKIVRMLYPEVDKQLSWLLQYAPSRL
EEEEEEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TGTGSCVFAEFSSKKEAELVLEQLPDTVSAFVAKGRNISPLKETLAEYQSAHPQSI
CCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA