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The map label for this gene is pleD [H]

Identifier: 218708722

GI number: 218708722

Start: 734547

End: 735785

Strand: Reverse

Name: pleD [H]

Synonym: VS_0701

Alternate gene names: 218708722

Gene position: 735785-734547 (Counterclockwise)

Preceding gene: 218708724

Following gene: 218708712

Centisome position: 22.3

GC content: 43.5

Gene sequence:

>1239_bases
TTGAGTGATAAAATACTAGTAGTCGAAGATAGTCGTGCATTCAGAAACTACCTGTACCAGCAATTTAAAAATGATGGCTA
TGAGGTTGCGCTGGCTGAATCAGTGGCTGAAGCTAAAGCTATCTTAGAACAAGATACGGATTTCTTGTGCGCGGTGCTCG
ATTACTGCTTACCCGATGGTCAAGATGGCGAGATCATTGATTTGGTACTCGGCTATCAACAAAAAATCATAGTCCTAACC
GGCATGTTCAACAACACACTTCGAGAACAAGTACTCGCGAAGGGTGTGATTGATTACATCCTCAAAGACAGCATGTCATC
AGTCAGTTATTTGCTTCCTCTGGTTAATCGAATCTCAAATAACCGATATCATAAAGCCTTGGTTGTTGATGATTCTGCTG
TGGTTCGTCGATATGTAGTGCAACTTCTTGAGCACCAATATATTCAAACGATTCAAGCGGAAGATGGCGAACAAGCACTA
GAGCTTCTCCAAAACGACCCCGACATCACTTTCGTCGTTACCGATCATGACATGCCAAACAAAGATGGCATTTCGATGAC
CCGTGACATTCGAGTACATCATGATCGCAATCAGCTCGCTATTCTTGGGCTATCTGGCAGTGACGACCGAACCATGACAG
CTCGTTTCCTTAAGGCTGGTGCCAACGACTTCCTTTACAAGCCATTTAACCAAGAAGAGTTTTTCTGCCGTATTCATCAG
CTACTCGATATGAAAGAGGCCACCAATGAACTATTTCGTCATGCAAATGAAGATGCTCTGACCGGGCTTTGGAACCGTCG
TTATCTATTCAACCAAACATGTAAAGGCTGCGAACATCGCAGCATCGCCATGATGGATATCGACTTCTTTAAAAAGGTGA
ATGATACCTACGGTCATGATGGTGGCGATGCAGTACTCATTGAAGTCGGAAAGATCATCAAAAATCATTTTAAAGATGAT
GTCGCCGTTCGTTTCGGCGGTGAAGAGTTCTGCATTCAATCATGCCGACCATTTGAAGAATTTGTCGACACGCTAGAGTC
AATGAGGATTGCGATACAAAACCAAGAAGTTATTCATGACTCACAACGGATTAAAGTCAGCATGAGTATCGGGTTAAGCG
ACTTAGTTGGCAGTTTGGATGAGCAAATAAAAGCCGCGGACGAACTACTTTATACTGCAAAAGAACAAGGTAGGAACCAA
CTGGTCTTTGCGCGCAATAACATGCAAATCGACCACTAG

Upstream 100 bases:

>100_bases
AAATGATCTGGAGCCCAGCTAAATAGCTTTAACTTCATTAGCCACTTACTATTCTGATAGTATTGTTAGAGAGTATTTAA
CCTATAAGTAGGGATAACTT

Downstream 100 bases:

>100_bases
AAATTCGTTTTTGTTTCCGTTTTTAGCATTCCAAAATAGACAGTTCACTCAAACAAAAAAAGCTCAAGACGATAATCTTG
AGCTTCTTATTCTAAGGGCA

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSDKILVVEDSRAFRNYLYQQFKNDGYEVALAESVAEAKAILEQDTDFLCAVLDYCLPDGQDGEIIDLVLGYQQKIIVLT
GMFNNTLREQVLAKGVIDYILKDSMSSVSYLLPLVNRISNNRYHKALVVDDSAVVRRYVVQLLEHQYIQTIQAEDGEQAL
ELLQNDPDITFVVTDHDMPNKDGISMTRDIRVHHDRNQLAILGLSGSDDRTMTARFLKAGANDFLYKPFNQEEFFCRIHQ
LLDMKEATNELFRHANEDALTGLWNRRYLFNQTCKGCEHRSIAMMDIDFFKKVNDTYGHDGGDAVLIEVGKIIKNHFKDD
VAVRFGGEEFCIQSCRPFEEFVDTLESMRIAIQNQEVIHDSQRIKVSMSIGLSDLVGSLDEQIKAADELLYTAKEQGRNQ
LVFARNNMQIDH

Sequences:

>Translated_412_residues
MSDKILVVEDSRAFRNYLYQQFKNDGYEVALAESVAEAKAILEQDTDFLCAVLDYCLPDGQDGEIIDLVLGYQQKIIVLT
GMFNNTLREQVLAKGVIDYILKDSMSSVSYLLPLVNRISNNRYHKALVVDDSAVVRRYVVQLLEHQYIQTIQAEDGEQAL
ELLQNDPDITFVVTDHDMPNKDGISMTRDIRVHHDRNQLAILGLSGSDDRTMTARFLKAGANDFLYKPFNQEEFFCRIHQ
LLDMKEATNELFRHANEDALTGLWNRRYLFNQTCKGCEHRSIAMMDIDFFKKVNDTYGHDGGDAVLIEVGKIIKNHFKDD
VAVRFGGEEFCIQSCRPFEEFVDTLESMRIAIQNQEVIHDSQRIKVSMSIGLSDLVGSLDEQIKAADELLYTAKEQGRNQ
LVFARNNMQIDH
>Mature_411_residues
SDKILVVEDSRAFRNYLYQQFKNDGYEVALAESVAEAKAILEQDTDFLCAVLDYCLPDGQDGEIIDLVLGYQQKIIVLTG
MFNNTLREQVLAKGVIDYILKDSMSSVSYLLPLVNRISNNRYHKALVVDDSAVVRRYVVQLLEHQYIQTIQAEDGEQALE
LLQNDPDITFVVTDHDMPNKDGISMTRDIRVHHDRNQLAILGLSGSDDRTMTARFLKAGANDFLYKPFNQEEFFCRIHQL
LDMKEATNELFRHANEDALTGLWNRRYLFNQTCKGCEHRSIAMMDIDFFKKVNDTYGHDGGDAVLIEVGKIIKNHFKDDV
AVRFGGEEFCIQSCRPFEEFVDTLESMRIAIQNQEVIHDSQRIKVSMSIGLSDLVGSLDEQIKAADELLYTAKEQGRNQL
VFARNNMQIDH

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=182, Percent_Identity=33.5164835164835, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87082007, Length=167, Percent_Identity=38.3233532934132, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI145693134, Length=171, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=29.746835443038, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787262, Length=189, Percent_Identity=31.7460317460317, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787816, Length=167, Percent_Identity=33.5329341317365, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787802, Length=173, Percent_Identity=30.0578034682081, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1787541, Length=195, Percent_Identity=30.2564102564103, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=32.5, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=33.7662337662338, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790863, Length=116, Percent_Identity=30.1724137931034, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI87081977, Length=120, Percent_Identity=35.8333333333333, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 47129; Mature: 46998

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKILVVEDSRAFRNYLYQQFKNDGYEVALAESVAEAKAILEQDTDFLCAVLDYCLPDG
CCCCEEEEECCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
QDGEIIDLVLGYQQKIIVLTGMFNNTLREQVLAKGVIDYILKDSMSSVSYLLPLVNRISN
CCCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRYHKALVVDDSAVVRRYVVQLLEHQYIQTIQAEDGEQALELLQNDPDITFVVTDHDMPN
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCC
KDGISMTRDIRVHHDRNQLAILGLSGSDDRTMTARFLKAGANDFLYKPFNQEEFFCRIHQ
CCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LLDMKEATNELFRHANEDALTGLWNRRYLFNQTCKGCEHRSIAMMDIDFFKKVNDTYGHD
HHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCC
GGDAVLIEVGKIIKNHFKDDVAVRFGGEEFCIQSCRPFEEFVDTLESMRIAIQNQEVIHD
CCCEEEHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCC
SQRIKVSMSIGLSDLVGSLDEQIKAADELLYTAKEQGRNQLVFARNNMQIDH
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
SDKILVVEDSRAFRNYLYQQFKNDGYEVALAESVAEAKAILEQDTDFLCAVLDYCLPDG
CCCEEEEECCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
QDGEIIDLVLGYQQKIIVLTGMFNNTLREQVLAKGVIDYILKDSMSSVSYLLPLVNRISN
CCCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NRYHKALVVDDSAVVRRYVVQLLEHQYIQTIQAEDGEQALELLQNDPDITFVVTDHDMPN
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCC
KDGISMTRDIRVHHDRNQLAILGLSGSDDRTMTARFLKAGANDFLYKPFNQEEFFCRIHQ
CCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
LLDMKEATNELFRHANEDALTGLWNRRYLFNQTCKGCEHRSIAMMDIDFFKKVNDTYGHD
HHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCCC
GGDAVLIEVGKIIKNHFKDDVAVRFGGEEFCIQSCRPFEEFVDTLESMRIAIQNQEVIHD
CCCEEEHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHCC
SQRIKVSMSIGLSDLVGSLDEQIKAADELLYTAKEQGRNQLVFARNNMQIDH
CCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA