Click here to switch to the map view.

The map label for this gene is rlmG

Identifier: 218708712

GI number: 218708712

Start: 724970

End: 726121

Strand: Reverse

Name: rlmG

Synonym: VS_0691

Alternate gene names: 218708712

Gene position: 726121-724970 (Counterclockwise)

Preceding gene: 218708722

Following gene: 218708711

Centisome position: 22.01

GC content: 42.36

Gene sequence:

>1152_bases
ATGAAAACCGAACTTACTCTTCTCGATAGAACTTTGACCTTACATCGTTTCCCCAACCGTTCAAATGAAACCCTTCAAGC
TTGGGATGCGGGCGACGAATACATTATTAGTCACGTTGAAGAGATGAATCTTGAACCTGGCAAGCACATCCTGATCATGA
ACGATAGCTTTGGTGCCCTGTCAGCATGGTTCTCTAAAGATCATGATGTCACCATGATGAGCGACTCGTTTATCTCTCAT
CGCGGTGCGCTAAAAAACTTGCAGCGCAATCAAAGTAACCGTGTGAACTTCCTGAATACGATGGATGATATCCCACACGG
TATCGATCTTGTGATCATGCAACTACCGAAAACAAACCGTCATCTAGTATGGCAATTGAGCCAACTACGCCAAGCGTTGC
CAGAAGGCTGCCAAGTCATCGGTGTAAACAAAGTAAAAGATATCCACACATCTACACTTAATATTTTCGAAAAGTACCTA
GGTGAAACCAAAACTTCTCTAGCGAAGAAGAAACATCGCTTGGTTTTCTCTTCACCAAATTGTCAGCCGATTCAAACGGT
CGAACCTTTTGTTGAATGGGATGTAGACGGTGAAGATATTCGCCTGAAAAATTTACCGAACGTTTACTCAGGCGAGGCAC
TCGATCAAGGCGCTCGCTATATGCTAGAGCACATCCCTCAAGATCCTGAACTGCGTCATATTATCGACCTAGGTTGTGGC
AACGGTGTATTGAGTGTAAAAGCTGGGCAATTAAACCCACAAGCGCGTATCACCTGTGTTGATGAAAGCTTTATGGCAGT
GGAATCAGCGCGCCAAAATATTAAAGATAACCTTGGCGAAGAAGGTAATTTCCAGTTCATCGCCAATAACTGTTTAGATG
GTTTTAAGAAAAACAGCACTTACTTAGTTATGTGTAACCCTCCGTTCCACCAACAACAAGCAATTACAGATCACATCGCA
TGGCAAATGTTCTGTGATGCAAAGCATGTTCTTAGCAACGGAGGCAAATTAATTGTTATTGGTAACCGACACCTCGGATA
CGATGTCAAACTAGCAAGATTATTTGGTGAAGCAAACGTTGAAACGCTTGAACTGAACCAGAAATTTGAGATATTACAAG
CAACAAGAGAACCTGCGAATTTTAATAAATAA

Upstream 100 bases:

>100_bases
TTCATAACGAACGACTGAGGTTTGGTTGAGTTTTTTCTATCTTGTTCAGGCTGCATTTGAGACAATATCTTTGTTATTTC
TTACATAAGTATCTCAAGCT

Downstream 100 bases:

>100_bases
GCAGAAACGACGTCACAAGAATTTAAGATACCGAGCTCCTGGTGTGAATTTAGAAAGGATAAAGGAATGAAAAAACTGAT
TTTGGCTGCTTCTATTTTGG

Product: putative ribosomal RNA small subunit methyltransferase D

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKTELTLLDRTLTLHRFPNRSNETLQAWDAGDEYIISHVEEMNLEPGKHILIMNDSFGALSAWFSKDHDVTMMSDSFISH
RGALKNLQRNQSNRVNFLNTMDDIPHGIDLVIMQLPKTNRHLVWQLSQLRQALPEGCQVIGVNKVKDIHTSTLNIFEKYL
GETKTSLAKKKHRLVFSSPNCQPIQTVEPFVEWDVDGEDIRLKNLPNVYSGEALDQGARYMLEHIPQDPELRHIIDLGCG
NGVLSVKAGQLNPQARITCVDESFMAVESARQNIKDNLGEEGNFQFIANNCLDGFKKNSTYLVMCNPPFHQQQAITDHIA
WQMFCDAKHVLSNGGKLIVIGNRHLGYDVKLARLFGEANVETLELNQKFEILQATREPANFNK

Sequences:

>Translated_383_residues
MKTELTLLDRTLTLHRFPNRSNETLQAWDAGDEYIISHVEEMNLEPGKHILIMNDSFGALSAWFSKDHDVTMMSDSFISH
RGALKNLQRNQSNRVNFLNTMDDIPHGIDLVIMQLPKTNRHLVWQLSQLRQALPEGCQVIGVNKVKDIHTSTLNIFEKYL
GETKTSLAKKKHRLVFSSPNCQPIQTVEPFVEWDVDGEDIRLKNLPNVYSGEALDQGARYMLEHIPQDPELRHIIDLGCG
NGVLSVKAGQLNPQARITCVDESFMAVESARQNIKDNLGEEGNFQFIANNCLDGFKKNSTYLVMCNPPFHQQQAITDHIA
WQMFCDAKHVLSNGGKLIVIGNRHLGYDVKLARLFGEANVETLELNQKFEILQATREPANFNK
>Mature_383_residues
MKTELTLLDRTLTLHRFPNRSNETLQAWDAGDEYIISHVEEMNLEPGKHILIMNDSFGALSAWFSKDHDVTMMSDSFISH
RGALKNLQRNQSNRVNFLNTMDDIPHGIDLVIMQLPKTNRHLVWQLSQLRQALPEGCQVIGVNKVKDIHTSTLNIFEKYL
GETKTSLAKKKHRLVFSSPNCQPIQTVEPFVEWDVDGEDIRLKNLPNVYSGEALDQGARYMLEHIPQDPELRHIIDLGCG
NGVLSVKAGQLNPQARITCVDESFMAVESARQNIKDNLGEEGNFQFIANNCLDGFKKNSTYLVMCNPPFHQQQAITDHIA
WQMFCDAKHVLSNGGKLIVIGNRHLGYDVKLARLFGEANVETLELNQKFEILQATREPANFNK

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family

Homologues:

Organism=Escherichia coli, GI87082206, Length=371, Percent_Identity=40.7008086253369, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1790830, Length=288, Percent_Identity=29.1666666666667, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMG_VIBSL (B7VKD0)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416333.1
- ProteinModelPortal:   B7VKD0
- GeneID:   7160310
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0691
- HOGENOM:   HBG321931
- OMA:   NRHLGYH
- ProtClustDB:   CLSK874746
- GO:   GO:0005737
- HAMAP:   MF_01859
- InterPro:   IPR017237
- InterPro:   IPR007848
- PIRSF:   PIRSF037565

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.174

Molecular weight: Translated: 43475; Mature: 43475

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTELTLLDRTLTLHRFPNRSNETLQAWDAGDEYIISHVEEMNLEPGKHILIMNDSFGAL
CCCCEEEHHHEEEEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHH
SAWFSKDHDVTMMSDSFISHRGALKNLQRNQSNRVNFLNTMDDIPHGIDLVIMQLPKTNR
HHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEEECCCCCC
HLVWQLSQLRQALPEGCQVIGVNKVKDIHTSTLNIFEKYLGETKTSLAKKKHRLVFSSPN
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
CQPIQTVEPFVEWDVDGEDIRLKNLPNVYSGEALDQGARYMLEHIPQDPELRHIIDLGCG
CCCCHHCCHHHEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEEEECCC
NGVLSVKAGQLNPQARITCVDESFMAVESARQNIKDNLGEEGNFQFIANNCLDGFKKNST
CCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCE
YLVMCNPPFHQQQAITDHIAWQMFCDAKHVLSNGGKLIVIGNRHLGYDVKLARLFGEANV
EEEEECCCCCHHHHHHHHHHHHHHHCHHHHHCCCCEEEEECCCCCCCCEEEHHHHCCCCC
ETLELNQKFEILQATREPANFNK
EEEECCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTELTLLDRTLTLHRFPNRSNETLQAWDAGDEYIISHVEEMNLEPGKHILIMNDSFGAL
CCCCEEEHHHEEEEEECCCCCCCCEEECCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHH
SAWFSKDHDVTMMSDSFISHRGALKNLQRNQSNRVNFLNTMDDIPHGIDLVIMQLPKTNR
HHHHCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEEECCCCCC
HLVWQLSQLRQALPEGCQVIGVNKVKDIHTSTLNIFEKYLGETKTSLAKKKHRLVFSSPN
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCC
CQPIQTVEPFVEWDVDGEDIRLKNLPNVYSGEALDQGARYMLEHIPQDPELRHIIDLGCG
CCCCHHCCHHHEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHEEEECCC
NGVLSVKAGQLNPQARITCVDESFMAVESARQNIKDNLGEEGNFQFIANNCLDGFKKNST
CCEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCE
YLVMCNPPFHQQQAITDHIAWQMFCDAKHVLSNGGKLIVIGNRHLGYDVKLARLFGEANV
EEEEECCCCCHHHHHHHHHHHHHHHCHHHHHCCCCEEEEECCCCCCCCEEEHHHHCCCCC
ETLELNQKFEILQATREPANFNK
EEEECCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA