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The map label for this gene is mltF [H]

Identifier: 218708684

GI number: 218708684

Start: 688370

End: 689941

Strand: Reverse

Name: mltF [H]

Synonym: VS_0663

Alternate gene names: 218708684

Gene position: 689941-688370 (Counterclockwise)

Preceding gene: 218708687

Following gene: 218708683

Centisome position: 20.91

GC content: 46.25

Gene sequence:

>1572_bases
TTGATGCCTAAATTTACGCTCATCTTTTCGTCTAAGTTCCTTCTGCTTGCCATTGCCCTAGTTGGGTTGGCTGGATGCCA
GATTGAGTCAGAACCTAAAAGTGTCCTTGAGCAGATACGAGAGCGAGGCGTTCTTCGCGTCGGCACCCTAAATAACCAAC
TCTCTTATTACATTGGTCCCGATGGCCCTGCTGGTTTGGATTACGAGTTAGCTCGTGAATTTGCGCAGGAGCTTGGTGTA
CAGCTTGAGATTAAACCCGCTTACCGTTTATCCGGCCTATTTCCAGCCTTAAAGAAAGGCGAGATCGATCTTATCGCGAC
AGGATTAACGCAAAACAATAACCTGACACGCGCTTATCGCGCTGCGCCCGCCTATTACTATGTAAGCCAGCAAGTGGTGT
ATAAAAAAGGCCAGTGGCGCCCAAGAAACCTTCAACAACTGATCAAGTTCGAGAATAAGCGTCAAGCTGATTTCGTAAAA
GCACAAACTGAATCAGAAGAGCCGGCATCTACCGAAACATTGACGCCATCTTTGGTGGTAGTGAAGGACTCACATTTTGA
GCCAACGCTAAAACAGATCCAGAACAAGCACGATGACTTTATCTACGATGCAATAGGTAATGCTGACATCAACGACCTGC
TAAAAGAAGTCTCTACAGGTGAGCGCTTGTTTACTGTTGCAGACTCGATTGAGCTGTCACTCGCACAACGTTTATACCCA
GACGTAGCCTTAGCTTTTGAACTGACGGAAGATCAACCTATCTCTTGGTATTTACAGAAGTCTGAAGATGAAAGCCTTTA
TGCATTACTGATTGAGTTCTTTGGTAACCTAAAACAGTCTGGTGAACTGGCTTCACTGGAAGAGAAGTACATCGGTCATA
TCGGCACCTTTGATTACGTTGATACTCGTGCATTTATCCGCGCATTGGATAGCAAGCTGCCAAAATGGTCGCCGCTATTT
CAGAAATATTCTCAAGAATTTGACTGGCGCTTGATTGCCGCTTTAGCATACCAAGAGTCACACTGGAATCCTGTCGCACG
CTCTCCAACAGGTGTTCGAGGCATGATGATGCTGACACTGCCTACTGCGAAGAGCGTTGGTGTAACCAATCGTCTCGACC
CGAAACAGTCGGTACAAGGCGGTGTTGAATATTTACGTCGTATCGTGAATCGAGTGCCCGACTCAATCACTCAGCATGAA
AAGATCTGGTTTGCGCTTGCCTCATATAACGTAGGTTACGGGCACATGATGGATGCTCGTCGCTTAACCAAAGCACAAGG
CGGTGATCCTGATGCTTGGGGTGATGTGAAAGACAGGCTTCCTCTGCTAAGACAGAAGAAGTACTACAGCAAAACTCGTT
ACGGGTATGCGCGTGGTGATGAAGCAAGGAATTACGTTGAGAACATTCGCCGTTATTATCAGAGCATTATTGGCCATGTA
AATAAGCGTAATAAAGAGGAAGAAGCGAGCCTTGAAGGTTTGGTCGTTATCCCACCTCTAGAGGTATCAGATGCTGAATC
AACAATCAGTGAAACTGAATCCGCAGTGAACGACGCTGAGTCTTCCAAATAA

Upstream 100 bases:

>100_bases
TGCTGCATTTATCAGCACTTTTACAACTGTTTAAATAAACCTGCTTTGCCAGCCTTGCGGGGTTTGGTATAAAGGGGCTT
CCACTTTTCGAGATTTCATT

Downstream 100 bases:

>100_bases
AAGAAAAAGAGCAATGCCATTTAGCATTGCTCTTTTTTATTGCATCACGATAAATGACCAAAACAAACAAAACGCCTGCG
ATATCCAGTGTGTCATATTG

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MMPKFTLIFSSKFLLLAIALVGLAGCQIESEPKSVLEQIRERGVLRVGTLNNQLSYYIGPDGPAGLDYELAREFAQELGV
QLEIKPAYRLSGLFPALKKGEIDLIATGLTQNNNLTRAYRAAPAYYYVSQQVVYKKGQWRPRNLQQLIKFENKRQADFVK
AQTESEEPASTETLTPSLVVVKDSHFEPTLKQIQNKHDDFIYDAIGNADINDLLKEVSTGERLFTVADSIELSLAQRLYP
DVALAFELTEDQPISWYLQKSEDESLYALLIEFFGNLKQSGELASLEEKYIGHIGTFDYVDTRAFIRALDSKLPKWSPLF
QKYSQEFDWRLIAALAYQESHWNPVARSPTGVRGMMMLTLPTAKSVGVTNRLDPKQSVQGGVEYLRRIVNRVPDSITQHE
KIWFALASYNVGYGHMMDARRLTKAQGGDPDAWGDVKDRLPLLRQKKYYSKTRYGYARGDEARNYVENIRRYYQSIIGHV
NKRNKEEEASLEGLVVIPPLEVSDAESTISETESAVNDAESSK

Sequences:

>Translated_523_residues
MMPKFTLIFSSKFLLLAIALVGLAGCQIESEPKSVLEQIRERGVLRVGTLNNQLSYYIGPDGPAGLDYELAREFAQELGV
QLEIKPAYRLSGLFPALKKGEIDLIATGLTQNNNLTRAYRAAPAYYYVSQQVVYKKGQWRPRNLQQLIKFENKRQADFVK
AQTESEEPASTETLTPSLVVVKDSHFEPTLKQIQNKHDDFIYDAIGNADINDLLKEVSTGERLFTVADSIELSLAQRLYP
DVALAFELTEDQPISWYLQKSEDESLYALLIEFFGNLKQSGELASLEEKYIGHIGTFDYVDTRAFIRALDSKLPKWSPLF
QKYSQEFDWRLIAALAYQESHWNPVARSPTGVRGMMMLTLPTAKSVGVTNRLDPKQSVQGGVEYLRRIVNRVPDSITQHE
KIWFALASYNVGYGHMMDARRLTKAQGGDPDAWGDVKDRLPLLRQKKYYSKTRYGYARGDEARNYVENIRRYYQSIIGHV
NKRNKEEEASLEGLVVIPPLEVSDAESTISETESAVNDAESSK
>Mature_523_residues
MMPKFTLIFSSKFLLLAIALVGLAGCQIESEPKSVLEQIRERGVLRVGTLNNQLSYYIGPDGPAGLDYELAREFAQELGV
QLEIKPAYRLSGLFPALKKGEIDLIATGLTQNNNLTRAYRAAPAYYYVSQQVVYKKGQWRPRNLQQLIKFENKRQADFVK
AQTESEEPASTETLTPSLVVVKDSHFEPTLKQIQNKHDDFIYDAIGNADINDLLKEVSTGERLFTVADSIELSLAQRLYP
DVALAFELTEDQPISWYLQKSEDESLYALLIEFFGNLKQSGELASLEEKYIGHIGTFDYVDTRAFIRALDSKLPKWSPLF
QKYSQEFDWRLIAALAYQESHWNPVARSPTGVRGMMMLTLPTAKSVGVTNRLDPKQSVQGGVEYLRRIVNRVPDSITQHE
KIWFALASYNVGYGHMMDARRLTKAQGGDPDAWGDVKDRLPLLRQKKYYSKTRYGYARGDEARNYVENIRRYYQSIIGHV
NKRNKEEEASLEGLVVIPPLEVSDAESTISETESAVNDAESSK

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=456, Percent_Identity=47.3684210526316, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 59269; Mature: 59269

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPKFTLIFSSKFLLLAIALVGLAGCQIESEPKSVLEQIRERGVLRVGTLNNQLSYYIGP
CCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECC
DGPAGLDYELAREFAQELGVQLEIKPAYRLSGLFPALKKGEIDLIATGLTQNNNLTRAYR
CCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHCHHHCCCCEEEEEECCCCCCCCCHHHH
AAPAYYYVSQQVVYKKGQWRPRNLQQLIKFENKRQADFVKAQTESEEPASTETLTPSLVV
CCCHHEEHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCEEEE
VKDSHFEPTLKQIQNKHDDFIYDAIGNADINDLLKEVSTGERLFTVADSIELSLAQRLYP
EECCCCCHHHHHHCCCCCCEEHHCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCC
DVALAFELTEDQPISWYLQKSEDESLYALLIEFFGNLKQSGELASLEEKYIGHIGTFDYV
CEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHH
DTRAFIRALDSKLPKWSPLFQKYSQEFDWRLIAALAYQESHWNPVARSPTGVRGMMMLTL
HHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
PTAKSVGVTNRLDPKQSVQGGVEYLRRIVNRVPDSITQHEKIWFALASYNVGYGHMMDAR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCCCHHHHHH
RLTKAQGGDPDAWGDVKDRLPLLRQKKYYSKTRYGYARGDEARNYVENIRRYYQSIIGHV
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
NKRNKEEEASLEGLVVIPPLEVSDAESTISETESAVNDAESSK
HCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMPKFTLIFSSKFLLLAIALVGLAGCQIESEPKSVLEQIRERGVLRVGTLNNQLSYYIGP
CCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEEEECC
DGPAGLDYELAREFAQELGVQLEIKPAYRLSGLFPALKKGEIDLIATGLTQNNNLTRAYR
CCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHCHHHCCCCEEEEEECCCCCCCCCHHHH
AAPAYYYVSQQVVYKKGQWRPRNLQQLIKFENKRQADFVKAQTESEEPASTETLTPSLVV
CCCHHEEHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCEEEE
VKDSHFEPTLKQIQNKHDDFIYDAIGNADINDLLKEVSTGERLFTVADSIELSLAQRLYP
EECCCCCHHHHHHCCCCCCEEHHCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCC
DVALAFELTEDQPISWYLQKSEDESLYALLIEFFGNLKQSGELASLEEKYIGHIGTFDYV
CEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHH
DTRAFIRALDSKLPKWSPLFQKYSQEFDWRLIAALAYQESHWNPVARSPTGVRGMMMLTL
HHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
PTAKSVGVTNRLDPKQSVQGGVEYLRRIVNRVPDSITQHEKIWFALASYNVGYGHMMDAR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCCCHHHHHH
RLTKAQGGDPDAWGDVKDRLPLLRQKKYYSKTRYGYARGDEARNYVENIRRYYQSIIGHV
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
NKRNKEEEASLEGLVVIPPLEVSDAESTISETESAVNDAESSK
HCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA