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The map label for this gene is gltB [H]

Identifier: 218708508

GI number: 218708508

Start: 490211

End: 490945

Strand: Reverse

Name: gltB [H]

Synonym: VS_0472

Alternate gene names: 218708508

Gene position: 490945-490211 (Counterclockwise)

Preceding gene: 218708509

Following gene: 218708507

Centisome position: 14.88

GC content: 47.07

Gene sequence:

>735_bases
ATGGCTCTATATGATCCTAGTCTTGAAAAAGACAACTGTGGATTTGGTTTAATAGCGCAAATGGAAGGCCAACCGAGTCA
TAAGTTGGTACGAACTGCTATTTCAGCCCTCGATCGCATGACACACCGTGGTGGTATCGCCGCGGATGGTAAAACCGGAG
ATGGCTGTGGCTTATTGCTACAGAAGCCCGACTCTTATCTCAGAATTATTGCTGAAGAGAATAACTTCAAGCTCGGCAAG
CAATACGCTGTCGGCATGATTTTTTTCAGCCAAGACCCAATCAAAGCGCAATCGGCGCAAGACATCGTCAATAAAGAGCT
TGCTCAAGAGACTTTAACCGTAGCTGGCTGGCGTGTCGTGCCGACCAACACCGATGTTTTAGGCCCAATCGCCAAAGATT
CTGTTCCCAATATTCAACAGGTGTTTATCTCAGCCCCTGCGGGTTGGCGTGAACGCGATATTGAACGACGCCTTTATATC
GCTCGTCGTCGAATTGAAAAGCAGATCACCGAAGACAAAGATTTTTATATTTGTAGCCTGTCGACTCAAGTCATGGTCTA
CAAAGGCTTGTGTATGCCCGCCGATCTTCCGCGATTTTACCTCGATCTTGCTGACTTACGTATGGAATCAACGAATAGAA
TCAGCAATATGTCTGTTCCACCAGCGCTTTTCAACCAACACACAACCGCGTTGGCCACTTGCTCAACCATTCCGTTATTT
GGCGCACAATGGTGA

Upstream 100 bases:

>100_bases
GCTAGCTAAAAACAAACTATAAGTTCATAAAAAATAACAAGTACATGAATAGTTAGTCATTTACTGTCTGGATGACAGCG
AAGCAAAAGGGAGAATTGCA

Downstream 100 bases:

>100_bases
AATCAATACCATCGAGGGTAACCGTCAATGGGCCAAGGCTCGTGCCTATAAATTCTCTTCGCCGCTGCTGCCCGATTTAC
AAACGGCCGCACCTTTTGTG

Product: putative glutamate synthase precursor

Products: NA

Alternate protein names: Glutamate synthase subunit alpha; GLTS alpha chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MALYDPSLEKDNCGFGLIAQMEGQPSHKLVRTAISALDRMTHRGGIAADGKTGDGCGLLLQKPDSYLRIIAEENNFKLGK
QYAVGMIFFSQDPIKAQSAQDIVNKELAQETLTVAGWRVVPTNTDVLGPIAKDSVPNIQQVFISAPAGWRERDIERRLYI
ARRRIEKQITEDKDFYICSLSTQVMVYKGLCMPADLPRFYLDLADLRMESTNRISNMSVPPALFNQHTTALATCSTIPLF
GAQW

Sequences:

>Translated_244_residues
MALYDPSLEKDNCGFGLIAQMEGQPSHKLVRTAISALDRMTHRGGIAADGKTGDGCGLLLQKPDSYLRIIAEENNFKLGK
QYAVGMIFFSQDPIKAQSAQDIVNKELAQETLTVAGWRVVPTNTDVLGPIAKDSVPNIQQVFISAPAGWRERDIERRLYI
ARRRIEKQITEDKDFYICSLSTQVMVYKGLCMPADLPRFYLDLADLRMESTNRISNMSVPPALFNQHTTALATCSTIPLF
GAQW
>Mature_243_residues
ALYDPSLEKDNCGFGLIAQMEGQPSHKLVRTAISALDRMTHRGGIAADGKTGDGCGLLLQKPDSYLRIIAEENNFKLGKQ
YAVGMIFFSQDPIKAQSAQDIVNKELAQETLTVAGWRVVPTNTDVLGPIAKDSVPNIQQVFISAPAGWRERDIERRLYIA
RRRIEKQITEDKDFYICSLSTQVMVYKGLCMPADLPRFYLDLADLRMESTNRISNMSVPPALFNQHTTALATCSTIPLFG
AQW

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0067

COG function: function code E; Glutamate synthase domain 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=223, Percent_Identity=65.9192825112108, Blast_Score=317, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17570289, Length=212, Percent_Identity=35.8490566037736, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6320030, Length=226, Percent_Identity=34.070796460177, Blast_Score=125, Evalue=5e-30,
Organism=Drosophila melanogaster, GI28574881, Length=214, Percent_Identity=37.8504672897196, Blast_Score=138, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24665539, Length=214, Percent_Identity=37.8504672897196, Blast_Score=138, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR002932
- InterPro:   IPR006982
- InterPro:   IPR002489 [H]

Pfam domain/function: PF00310 GATase_2; PF04898 Glu_syn_central; PF01645 Glu_synthase; PF01493 GXGXG [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 27187; Mature: 27055

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALYDPSLEKDNCGFGLIAQMEGQPSHKLVRTAISALDRMTHRGGIAADGKTGDGCGLLL
CCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEE
QKPDSYLRIIAEENNFKLGKQYAVGMIFFSQDPIKAQSAQDIVNKELAQETLTVAGWRVV
ECCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
PTNTDVLGPIAKDSVPNIQQVFISAPAGWRERDIERRLYIARRRIEKQITEDKDFYICSL
CCCCCCCCCCCCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
STQVMVYKGLCMPADLPRFYLDLADLRMESTNRISNMSVPPALFNQHTTALATCSTIPLF
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHCCCCCC
GAQW
CCCC
>Mature Secondary Structure 
ALYDPSLEKDNCGFGLIAQMEGQPSHKLVRTAISALDRMTHRGGIAADGKTGDGCGLLL
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEE
QKPDSYLRIIAEENNFKLGKQYAVGMIFFSQDPIKAQSAQDIVNKELAQETLTVAGWRVV
ECCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
PTNTDVLGPIAKDSVPNIQQVFISAPAGWRERDIERRLYIARRRIEKQITEDKDFYICSL
CCCCCCCCCCCCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
STQVMVYKGLCMPADLPRFYLDLADLRMESTNRISNMSVPPALFNQHTTALATCSTIPLF
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHCCCCCC
GAQW
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 1673677; 2643092 [H]