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The map label for this gene is gltD [H]

Identifier: 218708509

GI number: 218708509

Start: 491376

End: 492845

Strand: Reverse

Name: gltD [H]

Synonym: VS_0473

Alternate gene names: 218708509

Gene position: 492845-491376 (Counterclockwise)

Preceding gene: 218708510

Following gene: 218708508

Centisome position: 14.94

GC content: 46.39

Gene sequence:

>1470_bases
ATGGGTAAGCCTACTGGATTTTTAGAACACGGTCGTGAGCTTCCAAAAAAGCTCGATCCGTCAGTTCGAATTGAAGACAA
CAAAGAGTTCGTACTTAACGAAGAGTTTGGTGAAAAGATCAATACTCAAGCCTCTCGTTGTATGGACTGTGGCGTACCTT
TCTGTCACAGCGGTTGTCCGATTGGTAACATCATCCCAGAATTCAATGATGCGGTTTACCGTGACAGCTGGGAAGAAGCT
TGGAACATTCTAAGCTCTACCAATAACTTCCCTGAATTTACAGGTCGTGTGTGCCCTGCTCCTTGTGAAAGCGCTTGTGT
TCTTGGTATCAACCAAGACCCAATCACTATCTGTAATATCGAGAAAACAATTGTAGAAACTGCGTACCGTGAAGGGTACG
CAAAACCTAAAATACCTCGTTCACGTACGGGTAAAACAGTTGCGGTTATCGGTTCAGGTCCTGCAGGCTTAGCCGCAGCT
GAGCAGCTAAACAGTGCCGGTCACTCTGTGACGGTATTTGAACGTGACGAGAAAGTCGGTGGCCTACTGCGTTTCGGTAT
CCCAGATTTCAAACTGGGTATGGATGTGATTGATCGTAAGATCAACCTAATGGCTGAAGCTGGCGTTGAGTTTAAAGTGA
ACCAACACATCGGTGTTGATGTTAATGCTCAACAGCTACGCCAAGAGTTTGATGTGGTATTGCTAACAGGTGGTTCTACG
GTTCCACGTGACCTGCCAATCCCAGGTCGTGAGCTGAAAGGCGTTCACTTTGCCATGGAATTCCTTGGTCAAAATAACCG
CCGTGCCAACGACTTAGATCTAAAAACAGAAGAGCTTCACGCTAAAGATAAGCACATTGTGGTTATCGGTGGTGGTGATA
CGGGGTCTGACTGTGTGGGCACATCAAACCGTCATAAAGCAGCAAGCATTACTCAGGTTGAGATCATGCCGATCCCACCA
GAGAAGCGCCCTGCTAATATGCCTTGGCCTCAATACCCAATGATCATGAAGACCACCACTTCTCACGAAGAAGGTTGTGA
ACGTCATTGGAACATCCTGACTAAAGAGTTCATCTCTGACGATAACGGTAATGTAACCGGACTTCGCATTGCTGACATCG
TTTGGCAAGATGCGAAACCAGGTGAACGTCCAGGTTTTGATGAAGTAGCAAACTCTGAACGTGTTATCCCTTGTGATATG
GCATTTCTAGCGATGGGCTTCTTACACCCAGAACCAACAGGTGTACTAGCACAGCTAGACATTAAACTGGACGAGCGCGG
TAACGTTGCTTCTGAAGGCTTTGCGACCAACCAGAAAGGCGTTTTCGCTGCTGGTGATATGCGTACTGGTCAGTCTCTGG
TTGTACGTTGTATTAATGAAGGTCGTGAATGTGCAATTGCCATTGATGACTTCTTAATGGGTAACTCAAACTTAGAAGCG
AAAGCTGATTCACTCATGCTTTCCGCATAA

Upstream 100 bases:

>100_bases
CATCACTAGTTAAAGTAATGCCACGCGACTACAAAGCAGTTCTTGAGAAGCGTAAAGCTGAAGCACAACAGGCACAAACG
GAAGAAGTGGAGGCAGTATA

Downstream 100 bases:

>100_bases
TATTTCTCGGGCTAATGTCGTTGACGTTAGCCTAGGGAAAAAGCAACACCCTTCCTAACTTTTATATTTGGCTAGCACTC
GATGCTAGCCTTTTTTCTAT

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MGKPTGFLEHGRELPKKLDPSVRIEDNKEFVLNEEFGEKINTQASRCMDCGVPFCHSGCPIGNIIPEFNDAVYRDSWEEA
WNILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNIEKTIVETAYREGYAKPKIPRSRTGKTVAVIGSGPAGLAAA
EQLNSAGHSVTVFERDEKVGGLLRFGIPDFKLGMDVIDRKINLMAEAGVEFKVNQHIGVDVNAQQLRQEFDVVLLTGGST
VPRDLPIPGRELKGVHFAMEFLGQNNRRANDLDLKTEELHAKDKHIVVIGGGDTGSDCVGTSNRHKAASITQVEIMPIPP
EKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISDDNGNVTGLRIADIVWQDAKPGERPGFDEVANSERVIPCDM
AFLAMGFLHPEPTGVLAQLDIKLDERGNVASEGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAIAIDDFLMGNSNLEA
KADSLMLSA

Sequences:

>Translated_489_residues
MGKPTGFLEHGRELPKKLDPSVRIEDNKEFVLNEEFGEKINTQASRCMDCGVPFCHSGCPIGNIIPEFNDAVYRDSWEEA
WNILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNIEKTIVETAYREGYAKPKIPRSRTGKTVAVIGSGPAGLAAA
EQLNSAGHSVTVFERDEKVGGLLRFGIPDFKLGMDVIDRKINLMAEAGVEFKVNQHIGVDVNAQQLRQEFDVVLLTGGST
VPRDLPIPGRELKGVHFAMEFLGQNNRRANDLDLKTEELHAKDKHIVVIGGGDTGSDCVGTSNRHKAASITQVEIMPIPP
EKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISDDNGNVTGLRIADIVWQDAKPGERPGFDEVANSERVIPCDM
AFLAMGFLHPEPTGVLAQLDIKLDERGNVASEGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAIAIDDFLMGNSNLEA
KADSLMLSA
>Mature_488_residues
GKPTGFLEHGRELPKKLDPSVRIEDNKEFVLNEEFGEKINTQASRCMDCGVPFCHSGCPIGNIIPEFNDAVYRDSWEEAW
NILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNIEKTIVETAYREGYAKPKIPRSRTGKTVAVIGSGPAGLAAAE
QLNSAGHSVTVFERDEKVGGLLRFGIPDFKLGMDVIDRKINLMAEAGVEFKVNQHIGVDVNAQQLRQEFDVVLLTGGSTV
PRDLPIPGRELKGVHFAMEFLGQNNRRANDLDLKTEELHAKDKHIVVIGGGDTGSDCVGTSNRHKAASITQVEIMPIPPE
KRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISDDNGNVTGLRIADIVWQDAKPGERPGFDEVANSERVIPCDMA
FLAMGFLHPEPTGVLAQLDIKLDERGNVASEGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAIAIDDFLMGNSNLEAK
ADSLMLSA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=491, Percent_Identity=23.4215885947047, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI111118983, Length=153, Percent_Identity=31.3725490196078, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI111118981, Length=153, Percent_Identity=31.3725490196078, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1789606, Length=455, Percent_Identity=34.7252747252747, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1788811, Length=448, Percent_Identity=32.5892857142857, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI87082180, Length=443, Percent_Identity=33.1828442437923, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788468, Length=435, Percent_Identity=27.816091954023, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789243, Length=428, Percent_Identity=28.9719626168224, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17570289, Length=493, Percent_Identity=44.8275862068966, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71984108, Length=484, Percent_Identity=23.9669421487603, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543860, Length=167, Percent_Identity=34.7305389221557, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320030, Length=482, Percent_Identity=44.8132780082988, Blast_Score=435, Evalue=1e-123,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=49.6907216494845, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=49.6907216494845, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=49.6907216494845, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=49.6907216494845, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24640763, Length=285, Percent_Identity=25.9649122807018, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18858217, Length=285, Percent_Identity=25.9649122807018, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53532; Mature: 53401

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPTGFLEHGRELPKKLDPSVRIEDNKEFVLNEEFGEKINTQASRCMDCGVPFCHSGCP
CCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECHHHHHHHHHHHHHHHHCCCCHHCCCCC
IGNIIPEFNDAVYRDSWEEAWNILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNI
CCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEECCCCCEEEECC
EKTIVETAYREGYAKPKIPRSRTGKTVAVIGSGPAGLAAAEQLNSAGHSVTVFERDEKVG
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCC
GLLRFGIPDFKLGMDVIDRKINLMAEAGVEFKVNQHIGVDVNAQQLRQEFDVVLLTGGST
CEEEECCCCHHHCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCEEEEECCCC
VPRDLPIPGRELKGVHFAMEFLGQNNRRANDLDLKTEELHAKDKHIVVIGGGDTGSDCVG
CCCCCCCCCHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCC
TSNRHKAASITQVEIMPIPPEKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISD
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
DNGNVTGLRIADIVWQDAKPGERPGFDEVANSERVIPCDMAFLAMGFLHPEPTGVLAQLD
CCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCCCEEEHHHHHHHHHCCCCCCCCEEEEEE
IKLDERGNVASEGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAIAIDDFLMGNSNLEA
EEECCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEEEEEHEEECCCCCCC
KADSLMLSA
CCCCEEECC
>Mature Secondary Structure 
GKPTGFLEHGRELPKKLDPSVRIEDNKEFVLNEEFGEKINTQASRCMDCGVPFCHSGCP
CCCCCHHHHHHHHHHHCCCCEEEECCCEEEECHHHHHHHHHHHHHHHHCCCCHHCCCCC
IGNIIPEFNDAVYRDSWEEAWNILSSTNNFPEFTGRVCPAPCESACVLGINQDPITICNI
CCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEEECCCCCEEEECC
EKTIVETAYREGYAKPKIPRSRTGKTVAVIGSGPAGLAAAEQLNSAGHSVTVFERDEKVG
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCC
GLLRFGIPDFKLGMDVIDRKINLMAEAGVEFKVNQHIGVDVNAQQLRQEFDVVLLTGGST
CEEEECCCCHHHCHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCEEEEECCCC
VPRDLPIPGRELKGVHFAMEFLGQNNRRANDLDLKTEELHAKDKHIVVIGGGDTGSDCVG
CCCCCCCCCHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCC
TSNRHKAASITQVEIMPIPPEKRPANMPWPQYPMIMKTTTSHEEGCERHWNILTKEFISD
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
DNGNVTGLRIADIVWQDAKPGERPGFDEVANSERVIPCDMAFLAMGFLHPEPTGVLAQLD
CCCCEEEEEEEEEEECCCCCCCCCCHHHHCCCCCEEEHHHHHHHHHCCCCCCCCEEEEEE
IKLDERGNVASEGFATNQKGVFAAGDMRTGQSLVVRCINEGRECAIAIDDFLMGNSNLEA
EEECCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCEEEEEEEHEEECCCCCCC
KADSLMLSA
CCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]