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The map label for this gene is pitA [C]

Identifier: 218708448

GI number: 218708448

Start: 417027

End: 418289

Strand: Reverse

Name: pitA [C]

Synonym: VS_0411

Alternate gene names: 218708448

Gene position: 418289-417027 (Counterclockwise)

Preceding gene: 218708449

Following gene: 218708447

Centisome position: 12.68

GC content: 46.32

Gene sequence:

>1263_bases
ATGGATATCCTTGCTAACTACGGCACTGTCCTGATTATTGTTGCAGCAGCTTTCGGTTTCTTGATGGCTATTGGTATTGG
TGCAAATGACGTTGCGAACGCAATGGGCACTTCAGTAGGCTCTAAAGCTCTAACCGTTAAACAAGCGATCATTATCGCAA
TGATCTTTGAGTTCGCAGGTGCTTACCTTGCGGGCGGCGAAGTAACAGACACGATCCGTAAAGGTGTTATCGAAACATCT
CTGTTTGCAGATCAACCTGACGTACTTGTGTACGGTATGATGTCTGCACTACTAGCTGCTGGTACATGGCTACTTCTAGC
TTCATACATGGGCTGGCCAGTATCAACGACTCACTCAATCATCGGTGCAATCATCGGTTTTGCTTGTGTGTCTGTGGGTA
CTGAAGCTGTAGATTGGAACAGTGTTCAAGGCATTGTGGGAAGTTGGATTGTCACACCATTGATTTCCGGTATCTTCGCC
TATCTCATATTTGTCAGTGCCCAGCGACTGATATTCGATACAGAAAACCCGCTATTCAATGCGAAGCGTTTTGTACCAGT
ATACATGTTCATTACTACAATGGTCATTGCACTTGTAACGATCAAGAAAGGTCTTAAGCACGTAGGCCTTCATCTAACCG
GTACTGAAGCATGGTTATGGGCTGCAGGTGTTTCTGCTGTCGTCATGATTGGCGGTTACTTATACATTCAGAAGAAGTTT
GCTAACCGCGAAGAAGACCACGGTTTTACTGGCGTAGAAGGTATCTTCAGCGTACTAATGGTTATCACTGCTTGTGCAAT
GGCATTCGCACACGGTTCAAACGATGTTGCCAACGCGATTGGTCCACTATCAGCGGTTGTTTCAACGGTTGAGCACATGG
GCGAAATCTCAGGCAAGAGCACGATCGCATGGTGGATTCTTCCACTAGGTGGTATTGGTATCGTTGTTGGTCTTGCAACA
ATGGGTCATAAAGTAATGGCAACCGTTGGTACTGGTATTACAGAACTAACTCCTAGCCGTGGCTTTGCAGCTCAACTAGC
GACCGCGTGTACGGTTGTACTTGCTTCTGGTACTGGCCTTCCGATTTCAACCACGCAAACATTGGTTGGTGCGGTATTAG
GTGTAGGCTTTGCCCGTGGTATCGCAGCTCTAAACTTAGGCGTTGTGCGTAACATCGTAGCTTCTTGGATTGTGACGTTA
CCTGCAGGTGCTCTACTTGCTGTTGTATTCTTCTACGCAATTCAGGCAATGTTTACTATGTAA

Upstream 100 bases:

>100_bases
ATTTTTTGAACAAACCACTTATGCGCTGAGTATTTCTCTCGCTGAGCGGTTTTTTGCGTCTAAATTTGTTCCGCTTGTTA
TCAAACAACTAGGTATTACG

Downstream 100 bases:

>100_bases
TAGCCCTGACGCTAGTCAATATTAGCGTCAGAAAACGGTGAGCTTCAGTAAAGATATGTAAATACCATGTCATTATTGAC
TCAAACGCACAGAAAGGGAG

Product: putative phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MDILANYGTVLIIVAAAFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAGAYLAGGEVTDTIRKGVIETS
LFADQPDVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSIIGAIIGFACVSVGTEAVDWNSVQGIVGSWIVTPLISGIFA
YLIFVSAQRLIFDTENPLFNAKRFVPVYMFITTMVIALVTIKKGLKHVGLHLTGTEAWLWAAGVSAVVMIGGYLYIQKKF
ANREEDHGFTGVEGIFSVLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEISGKSTIAWWILPLGGIGIVVGLAT
MGHKVMATVGTGITELTPSRGFAAQLATACTVVLASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVVRNIVASWIVTL
PAGALLAVVFFYAIQAMFTM

Sequences:

>Translated_420_residues
MDILANYGTVLIIVAAAFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAGAYLAGGEVTDTIRKGVIETS
LFADQPDVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSIIGAIIGFACVSVGTEAVDWNSVQGIVGSWIVTPLISGIFA
YLIFVSAQRLIFDTENPLFNAKRFVPVYMFITTMVIALVTIKKGLKHVGLHLTGTEAWLWAAGVSAVVMIGGYLYIQKKF
ANREEDHGFTGVEGIFSVLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEISGKSTIAWWILPLGGIGIVVGLAT
MGHKVMATVGTGITELTPSRGFAAQLATACTVVLASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVVRNIVASWIVTL
PAGALLAVVFFYAIQAMFTM
>Mature_420_residues
MDILANYGTVLIIVAAAFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAGAYLAGGEVTDTIRKGVIETS
LFADQPDVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSIIGAIIGFACVSVGTEAVDWNSVQGIVGSWIVTPLISGIFA
YLIFVSAQRLIFDTENPLFNAKRFVPVYMFITTMVIALVTIKKGLKHVGLHLTGTEAWLWAAGVSAVVMIGGYLYIQKKF
ANREEDHGFTGVEGIFSVLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEISGKSTIAWWILPLGGIGIVVGLAT
MGHKVMATVGTGITELTPSRGFAAQLATACTVVLASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVVRNIVASWIVTL
PAGALLAVVFFYAIQAMFTM

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=191, Percent_Identity=40.3141361256544, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI31543630, Length=191, Percent_Identity=40.8376963350785, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71981576, Length=451, Percent_Identity=35.4767184035477, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17539280, Length=208, Percent_Identity=42.7884615384615, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25146401, Length=207, Percent_Identity=41.0628019323672, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17557328, Length=506, Percent_Identity=25.296442687747, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32566716, Length=211, Percent_Identity=38.3886255924171, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17536725, Length=158, Percent_Identity=37.9746835443038, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319773, Length=190, Percent_Identity=39.4736842105263, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356511, Length=209, Percent_Identity=38.2775119617225, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 43738; Mature: 43738

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILANYGTVLIIVAAAFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAG
CCCHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AYLAGGEVTDTIRKGVIETSLFADQPDVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSI
CHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IGAIIGFACVSVGTEAVDWNSVQGIVGSWIVTPLISGIFAYLIFVSAQRLIFDTENPLFN
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
AKRFVPVYMFITTMVIALVTIKKGLKHVGLHLTGTEAWLWAAGVSAVVMIGGYLYIQKKF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHEEEHHH
ANREEDHGFTGVEGIFSVLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEISGKS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TIAWWILPLGGIGIVVGLATMGHKVMATVGTGITELTPSRGFAAQLATACTVVLASGTGL
EEEEEEEHHCHHHHHHHHHHHCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC
PISTTQTLVGAVLGVGFARGIAALNLGVVRNIVASWIVTLPAGALLAVVFFYAIQAMFTM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDILANYGTVLIIVAAAFGFLMAIGIGANDVANAMGTSVGSKALTVKQAIIIAMIFEFAG
CCCHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
AYLAGGEVTDTIRKGVIETSLFADQPDVLVYGMMSALLAAGTWLLLASYMGWPVSTTHSI
CHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
IGAIIGFACVSVGTEAVDWNSVQGIVGSWIVTPLISGIFAYLIFVSAQRLIFDTENPLFN
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
AKRFVPVYMFITTMVIALVTIKKGLKHVGLHLTGTEAWLWAAGVSAVVMIGGYLYIQKKF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHEEEHHH
ANREEDHGFTGVEGIFSVLMVITACAMAFAHGSNDVANAIGPLSAVVSTVEHMGEISGKS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
TIAWWILPLGGIGIVVGLATMGHKVMATVGTGITELTPSRGFAAQLATACTVVLASGTGL
EEEEEEEHHCHHHHHHHHHHHCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCC
PISTTQTLVGAVLGVGFARGIAALNLGVVRNIVASWIVTLPAGALLAVVFFYAIQAMFTM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]