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The map label for this gene is cca [H]

Identifier: 218708444

GI number: 218708444

Start: 412115

End: 413431

Strand: Reverse

Name: cca [H]

Synonym: VS_0407

Alternate gene names: 218708444

Gene position: 413431-412115 (Counterclockwise)

Preceding gene: 218708447

Following gene: 218708440

Centisome position: 12.53

GC content: 46.92

Gene sequence:

>1317_bases
TTGCTCGCAGTAAAGTCCAGCAGTGATAAAACATTTCGAGGTGATAAGTTGCAAATATACGATAGCCTACCAAAAGACAA
TGGGCTACAGCGCTATCTAGTCGGTGGAGCGGTACGCGATAAGCTGCTCAATATTGATAGTTATGATAGAGATTGGGTCG
TGGTAGGAAGTACCCCTCAAGAGATGGAAAGCCTTGGCTTTACTGCTGTCGGGAAAGATTTCCCTGTATTCTTACATCCA
AAAACCAAAGAAGAGCACGCACTGGCTCGCACTGAGAAAAAATCCGGTTCGGGTTATACCGGTTTCGAATGTTACTTTGC
ACCAGATGTGAGTTTGGAAGAAGATCTCATGCGTCGAGATCTAACGATCAACGCCATCGCTCAAGATGACAAAGGCCAAC
TTTACGATCCTTACCATGGCCAAAAAGATCTCTCTGATCGTATTCTAAGGCACGTCTCGGATGCCTTTGTTGAAGATCCT
CTTCGTGTGCTGCGTGTGGCTCGCTTCGCCGCCAAACTCCATTATCTCAACTTTACGGTTGCATCTGAAACCATGGTGAT
GATGAGTGAGATAGTGCAATCCGGTGAACTGGCACACCTCACTGCCGAACGAGTTTGGCAGGAGTGGCATAAATCTCTTA
GTACTCCCCACCCTGAAGTGTTCCTTTCTATACTGAAAGAGTGTGGCGCATTAGCTGTTGTGTTGCCTGAAATTGACGCT
CTATTTGGTGTGCCTCAACCTGAAAAATGGCACCCTGAGATCGATACGGGCATTCACACTCTGATGGTGGCTCAACAAGC
AGCACTCTTAAGCCAGTCATTGCCAGTACGCTTTGCCGCTCAAGTACATGATTTAGGTAAAGGCGTCACGCCAGAAAGCG
AGTGGCCAAGCCACAAAATGCATTGCCATACAGGCTTAAACATCATTAAGAAGCTGTGTGAAAGAGTCAGAGTGCCCAAT
GAATTCAGAGACCTCGCGTTGTTAGTGTGCGAGCAGCATTCCAATATCCATCGTGCAGGCGAGCTAAAGCCAACCACCTT
CCTCAAGGTTCTCAACAAGTTCGATGTTTGGCGTAAGCCAGATAGACTCGACGATATACTGCTTTGCTGCCAGGCCGATC
ACGCAGGCCGAAAAGGATTAGAAAATCAGCCGTATCCTCAAAAAGCGCGTTTTGAAGTGGCTTACCAAGCAGCACTTCAA
GTCGAAGTTAAAGCGATTATTGCCGATGGTTTTCAAGGTAAAGACATTCGAGAAGAACAAGAAAAACGCAGAGCTGCTGC
GATTGAGCGCGCGTTATCAGAACTCTCTAATAGTTAA

Upstream 100 bases:

>100_bases
AATAGCGAGAATTTGGTACAATCGAAAATGGCATCTCAACGAAGCCAAAATATTCCTTATACATGTGCTGCCCTATAATT
ACGATTAATGGAAAGTACCA

Downstream 100 bases:

>100_bases
TCTTCATTACACTAGTAAACACACATTAAAAAACCCGCGATTAAGCGGGTTTTTCGTTTCAACTTGTAGGGTGCTGCATA
AGCGCTTTTTACTGCATCAT

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MLAVKSSSDKTFRGDKLQIYDSLPKDNGLQRYLVGGAVRDKLLNIDSYDRDWVVVGSTPQEMESLGFTAVGKDFPVFLHP
KTKEEHALARTEKKSGSGYTGFECYFAPDVSLEEDLMRRDLTINAIAQDDKGQLYDPYHGQKDLSDRILRHVSDAFVEDP
LRVLRVARFAAKLHYLNFTVASETMVMMSEIVQSGELAHLTAERVWQEWHKSLSTPHPEVFLSILKECGALAVVLPEIDA
LFGVPQPEKWHPEIDTGIHTLMVAQQAALLSQSLPVRFAAQVHDLGKGVTPESEWPSHKMHCHTGLNIIKKLCERVRVPN
EFRDLALLVCEQHSNIHRAGELKPTTFLKVLNKFDVWRKPDRLDDILLCCQADHAGRKGLENQPYPQKARFEVAYQAALQ
VEVKAIIADGFQGKDIREEQEKRRAAAIERALSELSNS

Sequences:

>Translated_438_residues
MLAVKSSSDKTFRGDKLQIYDSLPKDNGLQRYLVGGAVRDKLLNIDSYDRDWVVVGSTPQEMESLGFTAVGKDFPVFLHP
KTKEEHALARTEKKSGSGYTGFECYFAPDVSLEEDLMRRDLTINAIAQDDKGQLYDPYHGQKDLSDRILRHVSDAFVEDP
LRVLRVARFAAKLHYLNFTVASETMVMMSEIVQSGELAHLTAERVWQEWHKSLSTPHPEVFLSILKECGALAVVLPEIDA
LFGVPQPEKWHPEIDTGIHTLMVAQQAALLSQSLPVRFAAQVHDLGKGVTPESEWPSHKMHCHTGLNIIKKLCERVRVPN
EFRDLALLVCEQHSNIHRAGELKPTTFLKVLNKFDVWRKPDRLDDILLCCQADHAGRKGLENQPYPQKARFEVAYQAALQ
VEVKAIIADGFQGKDIREEQEKRRAAAIERALSELSNS
>Mature_438_residues
MLAVKSSSDKTFRGDKLQIYDSLPKDNGLQRYLVGGAVRDKLLNIDSYDRDWVVVGSTPQEMESLGFTAVGKDFPVFLHP
KTKEEHALARTEKKSGSGYTGFECYFAPDVSLEEDLMRRDLTINAIAQDDKGQLYDPYHGQKDLSDRILRHVSDAFVEDP
LRVLRVARFAAKLHYLNFTVASETMVMMSEIVQSGELAHLTAERVWQEWHKSLSTPHPEVFLSILKECGALAVVLPEIDA
LFGVPQPEKWHPEIDTGIHTLMVAQQAALLSQSLPVRFAAQVHDLGKGVTPESEWPSHKMHCHTGLNIIKKLCERVRVPN
EFRDLALLVCEQHSNIHRAGELKPTTFLKVLNKFDVWRKPDRLDDILLCCQADHAGRKGLENQPYPQKARFEVAYQAALQ
VEVKAIIADGFQGKDIREEQEKRRAAAIERALSELSNS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=400, Percent_Identity=58.5, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI87081691, Length=322, Percent_Identity=25.4658385093168, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321016, Length=243, Percent_Identity=28.3950617283951, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281360112, Length=350, Percent_Identity=25.7142857142857, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357337, Length=350, Percent_Identity=25.7142857142857, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 49457; Mature: 49457

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAVKSSSDKTFRGDKLQIYDSLPKDNGLQRYLVGGAVRDKLLNIDSYDRDWVVVGSTPQ
CCEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCHH
EMESLGFTAVGKDFPVFLHPKTKEEHALARTEKKSGSGYTGFECYFAPDVSLEEDLMRRD
HHHHCCCEEECCCCCEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHCC
LTINAIAQDDKGQLYDPYHGQKDLSDRILRHVSDAFVEDPLRVLRVARFAAKLHYLNFTV
CEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEH
ASETMVMMSEIVQSGELAHLTAERVWQEWHKSLSTPHPEVFLSILKECGALAVVLPEIDA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEHHHHHH
LFGVPQPEKWHPEIDTGIHTLMVAQQAALLSQSLPVRFAAQVHDLGKGVTPESEWPSHKM
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCE
HCHTGLNIIKKLCERVRVPNEFRDLALLVCEQHSNIHRAGELKPTTFLKVLNKFDVWRKP
EHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCC
DRLDDILLCCQADHAGRKGLENQPYPQKARFEVAYQAALQVEVKAIIADGFQGKDIREEQ
CCHHHHHHEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EKRRAAAIERALSELSNS
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLAVKSSSDKTFRGDKLQIYDSLPKDNGLQRYLVGGAVRDKLLNIDSYDRDWVVVGSTPQ
CCEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCHH
EMESLGFTAVGKDFPVFLHPKTKEEHALARTEKKSGSGYTGFECYFAPDVSLEEDLMRRD
HHHHCCCEEECCCCCEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHCC
LTINAIAQDDKGQLYDPYHGQKDLSDRILRHVSDAFVEDPLRVLRVARFAAKLHYLNFTV
CEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEH
ASETMVMMSEIVQSGELAHLTAERVWQEWHKSLSTPHPEVFLSILKECGALAVVLPEIDA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEHHHHHH
LFGVPQPEKWHPEIDTGIHTLMVAQQAALLSQSLPVRFAAQVHDLGKGVTPESEWPSHKM
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCE
HCHTGLNIIKKLCERVRVPNEFRDLALLVCEQHSNIHRAGELKPTTFLKVLNKFDVWRKP
EHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCC
DRLDDILLCCQADHAGRKGLENQPYPQKARFEVAYQAALQVEVKAIIADGFQGKDIREEQ
CCHHHHHHEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
EKRRAAAIERALSELSNS
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]