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The map label for this gene is dnaG [H]

Identifier: 218708435

GI number: 218708435

Start: 404771

End: 406522

Strand: Reverse

Name: dnaG [H]

Synonym: VS_0398

Alternate gene names: 218708435

Gene position: 406522-404771 (Counterclockwise)

Preceding gene: 218708436

Following gene: 218708434

Centisome position: 12.32

GC content: 46.46

Gene sequence:

>1752_bases
ATGGCAGGACACATCCCGCGTAGTTTCATCGATGATCTCCTAGCGCGTCTCGACATTGTCGATATTGTGGACGCACGCGT
GAAACTTAAGAAAAAAGGCAAAAACTATGGTGCTTGTTGCCCATTTCACAACGAAAAGACCCCTTCTTTCAGCGTAAGCC
AAGAAAAACAGTTTTATCACTGCTTTGGTTGTGGCGTACACGGCAATGCTATCGACTTCATTATGGAGTTCGAACGTCTC
GATTTTGTTGAAGCGATTGAAGAGCTGGCCTCATTTTTAGGACTTGATGTTCCTAGAGAACAGCGCAGCGGTGAAATATC
TACGGCGCCAAGAGCCAACAGCGAACAAAAACGTAACCTCTACGATTTGATGGGCGGCATCAGTAATTTTTACCGCTCTC
AGCTGAAAATTTCAGCTAACAAACCTGCGATTGATTACCTAAAAAATCGCGGTCTTTCTGGTGAAATCGTACAGAAGTTT
GGCATTGGCTACGTCGCTGATGAATGGGACTTGGTTCGTAAGAACTTTGGCCAACAAAAAGAAGCCCAAGACATGCTCGT
TACTGGCGGCATGTTGATAGAAAACGATAAAGGCAACCGATACGATCGATTCCGTGGACGCGTGATGTTCCCGATTCGTG
ACCGTCGAGGCCGAGTGATCGGGTTTGGTGGTCGTGTCTTAGAAGACGGCACACCGAAATACCTGAACTCACCAGAAACG
CCTATCTTCCATAAAGGCAAAGAGCTTTACGGCCTTTATGAAGTGCTGCAAGCCTACCGTGAACCGCCGCAAATACTTGT
GGTTGAAGGTTACATGGATGTCGTGGCATTAGCGCAATATGGTGTTGATTACTCAGTGGCATCACTGGGCACCTCGACAA
CCGGTGACCACGTTCAAATGTTGTTCCGCCAAACCAGCACAGTGGTTTGTTGTTACGACGGTGACCGAGCAGGTAAAGAA
GCGGCGTGGCGCGCACTCGAAAATGCGCTTGAATACCTGAAAACAGGTAACACGCTCAAGTTTCTGTTCTTGCCAGACGG
TGAAGACCCAGACAGCTATATTAGAGAACACGGTCAGGCTGCTTTCGAACAGCTCGTCCACAATGCGACACCGCTTTCTA
CTTACTTGTTCGATAATCTTATTGAGATACACAAGTTGAACTTGGGAACAACGGAAGGGAAGTCGGCACTGCGAGCACAC
GCAAGCGCCTTGATTAACAAAATCCCTGACAGTTATTTCCAAGAACTGCTCGAGAAATTATTGGATGAGCGTACTGGTTT
TGATAACCAACTGAGACGAGCTCGTGTTCATACTAAGAACCCGACACCTCAACCGCATAAAGAACTGAAACGCACTCCAA
TGCGAGAAGTTATCGCTTTGCTTATCCAAAATCCGAGCTATGCTGATATGGTACCGGATTTATCAAGTGTCAAAGGCTTA
CAGTTGCCTGGACTAAGTTTATTCGTCGAAGTACTTGATAAATGCCACGCGCATCCCCATATCAACACAGGCCAATTATT
AGAGCATTGGCGACACAATAAACATGAGGCGCTTCTGTCTCGTCTCGCGAGCTGGGAAATCCCCCTCGACGAAGACAATC
AAGAAGACATATTTTTAGACTCATTGGACAATATCCTTGCCCAGTGCGTTGAAAAACAAATTGAAAACCTGCAGGCCAAA
GCAAGAAGTGTCGGTTTATCAGCCGAAGAAAAAAGGGAGCTACTAGCTTTAATGCTAGATCTAAAAGCGTAA

Upstream 100 bases:

>100_bases
AAGCCGTGCAATCCTTCGGAACGCACGGCTTGTTTGTATCTGTAACCTATTCAAACTATTATTATCCACACTCTCAGTTA
GTGCATTTTTCTAAGGTTTT

Downstream 100 bases:

>100_bases
CCCTGTTTGATTAGTCAGCATTTAATAAATTTGTTAACATAATTGGTTTGCATTTGAGAATGCAACGTCCTTCACCAGAC
CTGAAGTTGGATATCATCTA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MAGHIPRSFIDDLLARLDIVDIVDARVKLKKKGKNYGACCPFHNEKTPSFSVSQEKQFYHCFGCGVHGNAIDFIMEFERL
DFVEAIEELASFLGLDVPREQRSGEISTAPRANSEQKRNLYDLMGGISNFYRSQLKISANKPAIDYLKNRGLSGEIVQKF
GIGYVADEWDLVRKNFGQQKEAQDMLVTGGMLIENDKGNRYDRFRGRVMFPIRDRRGRVIGFGGRVLEDGTPKYLNSPET
PIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDHVQMLFRQTSTVVCCYDGDRAGKE
AAWRALENALEYLKTGNTLKFLFLPDGEDPDSYIREHGQAAFEQLVHNATPLSTYLFDNLIEIHKLNLGTTEGKSALRAH
ASALINKIPDSYFQELLEKLLDERTGFDNQLRRARVHTKNPTPQPHKELKRTPMREVIALLIQNPSYADMVPDLSSVKGL
QLPGLSLFVEVLDKCHAHPHINTGQLLEHWRHNKHEALLSRLASWEIPLDEDNQEDIFLDSLDNILAQCVEKQIENLQAK
ARSVGLSAEEKRELLALMLDLKA

Sequences:

>Translated_583_residues
MAGHIPRSFIDDLLARLDIVDIVDARVKLKKKGKNYGACCPFHNEKTPSFSVSQEKQFYHCFGCGVHGNAIDFIMEFERL
DFVEAIEELASFLGLDVPREQRSGEISTAPRANSEQKRNLYDLMGGISNFYRSQLKISANKPAIDYLKNRGLSGEIVQKF
GIGYVADEWDLVRKNFGQQKEAQDMLVTGGMLIENDKGNRYDRFRGRVMFPIRDRRGRVIGFGGRVLEDGTPKYLNSPET
PIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDHVQMLFRQTSTVVCCYDGDRAGKE
AAWRALENALEYLKTGNTLKFLFLPDGEDPDSYIREHGQAAFEQLVHNATPLSTYLFDNLIEIHKLNLGTTEGKSALRAH
ASALINKIPDSYFQELLEKLLDERTGFDNQLRRARVHTKNPTPQPHKELKRTPMREVIALLIQNPSYADMVPDLSSVKGL
QLPGLSLFVEVLDKCHAHPHINTGQLLEHWRHNKHEALLSRLASWEIPLDEDNQEDIFLDSLDNILAQCVEKQIENLQAK
ARSVGLSAEEKRELLALMLDLKA
>Mature_582_residues
AGHIPRSFIDDLLARLDIVDIVDARVKLKKKGKNYGACCPFHNEKTPSFSVSQEKQFYHCFGCGVHGNAIDFIMEFERLD
FVEAIEELASFLGLDVPREQRSGEISTAPRANSEQKRNLYDLMGGISNFYRSQLKISANKPAIDYLKNRGLSGEIVQKFG
IGYVADEWDLVRKNFGQQKEAQDMLVTGGMLIENDKGNRYDRFRGRVMFPIRDRRGRVIGFGGRVLEDGTPKYLNSPETP
IFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDHVQMLFRQTSTVVCCYDGDRAGKEA
AWRALENALEYLKTGNTLKFLFLPDGEDPDSYIREHGQAAFEQLVHNATPLSTYLFDNLIEIHKLNLGTTEGKSALRAHA
SALINKIPDSYFQELLEKLLDERTGFDNQLRRARVHTKNPTPQPHKELKRTPMREVIALLIQNPSYADMVPDLSSVKGLQ
LPGLSLFVEVLDKCHAHPHINTGQLLEHWRHNKHEALLSRLASWEIPLDEDNQEDIFLDSLDNILAQCVEKQIENLQAKA
RSVGLSAEEKRELLALMLDLKA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=588, Percent_Identity=52.891156462585, Blast_Score=626, Evalue=1e-180,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65940; Mature: 65809

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGHIPRSFIDDLLARLDIVDIVDARVKLKKKGKNYGACCPFHNEKTPSFSVSQEKQFYH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCEEE
CFGCGVHGNAIDFIMEFERLDFVEAIEELASFLGLDVPREQRSGEISTAPRANSEQKRNL
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHH
YDLMGGISNFYRSQLKISANKPAIDYLKNRGLSGEIVQKFGIGYVADEWDLVRKNFGQQK
HHHHHHHHHHHHHHEEECCCCCHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCH
EAQDMLVTGGMLIENDKGNRYDRFRGRVMFPIRDRRGRVIGFGGRVLEDGTPKYLNSPET
HHHHHHHCCCEEEECCCCCCHHHHCCEEEEEEECCCCCEEEECCEEECCCCCHHCCCCCC
PIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDHVQM
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCEEHHHCCCCCCHHHHHH
LFRQTSTVVCCYDGDRAGKEAAWRALENALEYLKTGNTLKFLFLPDGEDPDSYIREHGQA
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
AFEQLVHNATPLSTYLFDNLIEIHKLNLGTTEGKSALRAHASALINKIPDSYFQELLEKL
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LDERTGFDNQLRRARVHTKNPTPQPHKELKRTPMREVIALLIQNPSYADMVPDLSSVKGL
HHHHCCCHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHCCCHHHCCCC
QLPGLSLFVEVLDKCHAHPHINTGQLLEHWRHNKHEALLSRLASWEIPLDEDNQEDIFLD
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
SLDNILAQCVEKQIENLQAKARSVGLSAEEKRELLALMLDLKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGHIPRSFIDDLLARLDIVDIVDARVKLKKKGKNYGACCPFHNEKTPSFSVSQEKQFYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCEEE
CFGCGVHGNAIDFIMEFERLDFVEAIEELASFLGLDVPREQRSGEISTAPRANSEQKRNL
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCHHHHHH
YDLMGGISNFYRSQLKISANKPAIDYLKNRGLSGEIVQKFGIGYVADEWDLVRKNFGQQK
HHHHHHHHHHHHHHEEECCCCCHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCH
EAQDMLVTGGMLIENDKGNRYDRFRGRVMFPIRDRRGRVIGFGGRVLEDGTPKYLNSPET
HHHHHHHCCCEEEECCCCCCHHHHCCEEEEEEECCCCCEEEECCEEECCCCCHHCCCCCC
PIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDHVQM
CCCCCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCEEHHHCCCCCCHHHHHH
LFRQTSTVVCCYDGDRAGKEAAWRALENALEYLKTGNTLKFLFLPDGEDPDSYIREHGQA
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHH
AFEQLVHNATPLSTYLFDNLIEIHKLNLGTTEGKSALRAHASALINKIPDSYFQELLEKL
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
LDERTGFDNQLRRARVHTKNPTPQPHKELKRTPMREVIALLIQNPSYADMVPDLSSVKGL
HHHHCCCHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHCCCHHHCCCC
QLPGLSLFVEVLDKCHAHPHINTGQLLEHWRHNKHEALLSRLASWEIPLDEDNQEDIFLD
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
SLDNILAQCVEKQIENLQAKARSVGLSAEEKRELLALMLDLKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3005129; 11677609 [H]