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The map label for this gene is pykF [H]

Identifier: 218708430

GI number: 218708430

Start: 396428

End: 397840

Strand: Reverse

Name: pykF [H]

Synonym: VS_0392

Alternate gene names: 218708430

Gene position: 397840-396428 (Counterclockwise)

Preceding gene: 218708433

Following gene: 218708429

Centisome position: 12.06

GC content: 44.09

Gene sequence:

>1413_bases
ATGAAAAAGACCAAAATCGTATGTACGATTGGCCCTAAAACTGAATCTGTAGAGAAGCTAACTGAACTAGTAGATGCTGG
CATGAACGTTATGCGTCTTAACTTCTCTCACGGTGATTTCGCAGAGCACGGCACTCGTATCGCGAACTTCCGTAAAGTAA
TGGAAAATAATGGTGAGCAACTTGCGATCCTTCTAGATACTAAAGGTCCAGAAATCCGTACTATCAAACTTGAAGATGGT
AACGACGTAGATCTAGTAGCTGGTCAAGAGTTCACTTTCACAACTGACGCAACAGTTGTTGGTAACAAAGACGTAGTAGC
AGTAACTTACCTAGGTTTCGCTAAGGACCTAACAGCAGGTAACACTATCCTTGTTGATGATGGCCTAATCGAAATGGAAG
TTATCGCAACAACAGAAACTGAAGTTAAGTGTAAAGTTCTTAACAACGGTGCTCTAGGCGAAAACAAAGGTGTTAACCTG
CCTGGCGTTTCTGTTCAACTTCCAGCTCTTTCTGAGAAAGACAAAGCTGACCTTAAGTTTGGTTGCGAGCAAGGCGTTGA
TTTCGTAGCTGCTTCTTTCATTCGTAAAGAAGAAGACGTTAAAGAAATCCGTGAGCTTCTAAACGCTAACGGCGGCGAGA
ACATCCACATCATTTCTAAGATTGAAAACCAAGAAGGTGTAGATAACTTCGATTCAATCCTTGAAGCTTCTGACGGCATC
ATGGTTGCTCGTGGTGACCTAGGTGTTGAAATCCCAGCTGAAGAAGTAATCTTCGCTCAGAAGATGATGATCGAGAAGTG
TAACCGCGCACGTAAGATGGTTATCACTGCAACTCAAATGCTTGACTCTATGATCAGCAACCCACGTCCAACTCGTGCAG
AAGCGGGTGACGTTGCGAACGCAATCATGGATGGTACTGATGCAGTAATGCTTTCTGGTGAAACTGCTAAAGGTAAGTAC
CCAGTTGAAGCTGTTACTATCATGGCTCAAATCGCGAACCGTACTGATTCAGCTCTTAAAGCTGAGCTAGGTTCTCGTCT
AGACAGCCCACGTCTACGCATCACTGAAGCAGTATGTAAAGGCGCTGTAGACACAGCAGAGAAGCTAGCTGCTCCTCTAA
TCGTTGTTGCAACTGAAGGCGGTAAGTCTGCACGTTCAGTACGTAAGTACTTCCCAACTGCAAACATCCTTGCTTTAACA
ACTAACGAAAAGACAGCTGCACAGCTAGTTCTTACTAAAGGTGTTAAGCCAGTTCTTGTTGACTCTATCGAGAACACAGA
CGCGTTCTACATCAACGGTAAAGAAATCGCTCTACAATCTGGCCTAGGTAACAAAGGCGACATCGTAGTTATGGTTTCTG
GTGCTCTAGTCGCTTCTGGTACTACAAACACAGCATCTGTTCACGTTCTATAA

Upstream 100 bases:

>100_bases
CGCTTTTACACAACAGGCACAAAATGTTGATGTAGCGGACCAATCTTCAGAACTTAAATAAATTTCAAATTGTAACAATA
CTTACCGGAGAGTACCTTCC

Downstream 100 bases:

>100_bases
GAATGAACCGATTCGCATAAAATATAAAAGAGGGCTTCGGCCCTCTTTTTTTATGAAACAAAATCTTGCCTAACTTTTCA
GTACGCTGTATTATCAATCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKTKIVCTIGPKTESVEKLTELVDAGMNVMRLNFSHGDFAEHGTRIANFRKVMENNGEQLAILLDTKGPEIRTIKLEDG
NDVDLVAGQEFTFTTDATVVGNKDVVAVTYLGFAKDLTAGNTILVDDGLIEMEVIATTETEVKCKVLNNGALGENKGVNL
PGVSVQLPALSEKDKADLKFGCEQGVDFVAASFIRKEEDVKEIRELLNANGGENIHIISKIENQEGVDNFDSILEASDGI
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKMVITATQMLDSMISNPRPTRAEAGDVANAIMDGTDAVMLSGETAKGKY
PVEAVTIMAQIANRTDSALKAELGSRLDSPRLRITEAVCKGAVDTAEKLAAPLIVVATEGGKSARSVRKYFPTANILALT
TNEKTAAQLVLTKGVKPVLVDSIENTDAFYINGKEIALQSGLGNKGDIVVMVSGALVASGTTNTASVHVL

Sequences:

>Translated_470_residues
MKKTKIVCTIGPKTESVEKLTELVDAGMNVMRLNFSHGDFAEHGTRIANFRKVMENNGEQLAILLDTKGPEIRTIKLEDG
NDVDLVAGQEFTFTTDATVVGNKDVVAVTYLGFAKDLTAGNTILVDDGLIEMEVIATTETEVKCKVLNNGALGENKGVNL
PGVSVQLPALSEKDKADLKFGCEQGVDFVAASFIRKEEDVKEIRELLNANGGENIHIISKIENQEGVDNFDSILEASDGI
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKMVITATQMLDSMISNPRPTRAEAGDVANAIMDGTDAVMLSGETAKGKY
PVEAVTIMAQIANRTDSALKAELGSRLDSPRLRITEAVCKGAVDTAEKLAAPLIVVATEGGKSARSVRKYFPTANILALT
TNEKTAAQLVLTKGVKPVLVDSIENTDAFYINGKEIALQSGLGNKGDIVVMVSGALVASGTTNTASVHVL
>Mature_470_residues
MKKTKIVCTIGPKTESVEKLTELVDAGMNVMRLNFSHGDFAEHGTRIANFRKVMENNGEQLAILLDTKGPEIRTIKLEDG
NDVDLVAGQEFTFTTDATVVGNKDVVAVTYLGFAKDLTAGNTILVDDGLIEMEVIATTETEVKCKVLNNGALGENKGVNL
PGVSVQLPALSEKDKADLKFGCEQGVDFVAASFIRKEEDVKEIRELLNANGGENIHIISKIENQEGVDNFDSILEASDGI
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKMVITATQMLDSMISNPRPTRAEAGDVANAIMDGTDAVMLSGETAKGKY
PVEAVTIMAQIANRTDSALKAELGSRLDSPRLRITEAVCKGAVDTAEKLAAPLIVVATEGGKSARSVRKYFPTANILALT
TNEKTAAQLVLTKGVKPVLVDSIENTDAFYINGKEIALQSGLGNKGDIVVMVSGALVASGTTNTASVHVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=48.3673469387755, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=48.3673469387755, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=48.2617586912065, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=46.9387755102041, Blast_Score=394, Evalue=1e-109,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=46.9387755102041, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=52.0833333333333, Blast_Score=262, Evalue=6e-70,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=52.0833333333333, Blast_Score=262, Evalue=6e-70,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=47.0338983050847, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=47.0338983050847, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=46.046511627907, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=77.8723404255319, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=476, Percent_Identity=38.0252100840336, Blast_Score=283, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=43.75, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=45.5670103092784, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=45.5670103092784, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=45.5670103092784, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=45.5670103092784, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6324923, Length=482, Percent_Identity=43.3609958506224, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=45.5301455301455, Blast_Score=357, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=45.6032719836401, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI28571814, Length=481, Percent_Identity=46.7775467775468, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24648966, Length=443, Percent_Identity=47.6297968397291, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=34.375, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24646914, Length=292, Percent_Identity=46.5753424657534, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50172; Mature: 50172

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPKTESVEKLTELVDAGMNVMRLNFSHGDFAEHGTRIANFRKVMENNGEQ
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCHHHHHHHHHHHCCCCE
LAILLDTKGPEIRTIKLEDGNDVDLVAGQEFTFTTDATVVGNKDVVAVTYLGFAKDLTAG
EEEEEECCCCEEEEEEECCCCCEEEEECCEEEEECCEEEECCCCEEEEEEECHHHHCCCC
NTILVDDGLIEMEVIATTETEVKCKVLNNGALGENKGVNLPGVSVQLPALSEKDKADLKF
CEEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHC
GCEQGVDFVAASFIRKEEDVKEIRELLNANGGENIHIISKIENQEGVDNFDSILEASDGI
CHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKMVITATQMLDSMISNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHCCCCCCCCCHHHHHH
AIMDGTDAVMLSGETAKGKYPVEAVTIMAQIANRTDSALKAELGSRLDSPRLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHC
GAVDTAEKLAAPLIVVATEGGKSARSVRKYFPTANILALTTNEKTAAQLVLTKGVKPVLV
CCHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEE
DSIENTDAFYINGKEIALQSGLGNKGDIVVMVSGALVASGTTNTASVHVL
ECCCCCCEEEECCCEEEEECCCCCCCCEEEEEECEEEECCCCCCEEEEEC
>Mature Secondary Structure
MKKTKIVCTIGPKTESVEKLTELVDAGMNVMRLNFSHGDFAEHGTRIANFRKVMENNGEQ
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCHHHHHHHHHHHCCCCE
LAILLDTKGPEIRTIKLEDGNDVDLVAGQEFTFTTDATVVGNKDVVAVTYLGFAKDLTAG
EEEEEECCCCEEEEEEECCCCCEEEEECCEEEEECCEEEECCCCEEEEEEECHHHHCCCC
NTILVDDGLIEMEVIATTETEVKCKVLNNGALGENKGVNLPGVSVQLPALSEKDKADLKF
CEEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHC
GCEQGVDFVAASFIRKEEDVKEIRELLNANGGENIHIISKIENQEGVDNFDSILEASDGI
CHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKMVITATQMLDSMISNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHCCCCCCCCCHHHHHH
AIMDGTDAVMLSGETAKGKYPVEAVTIMAQIANRTDSALKAELGSRLDSPRLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHC
GAVDTAEKLAAPLIVVATEGGKSARSVRKYFPTANILALTTNEKTAAQLVLTKGVKPVLV
CCHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEE
DSIENTDAFYINGKEIALQSGLGNKGDIVVMVSGALVASGTTNTASVHVL
ECCCCCCEEEECCCEEEEECCCCCCCCEEEEEECEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]