Click here to switch to the map view.

The map label for this gene is mdeA [H]

Identifier: 218708433

GI number: 218708433

Start: 401120

End: 402388

Strand: Reverse

Name: mdeA [H]

Synonym: VS_0395

Alternate gene names: 218708433

Gene position: 402388-401120 (Counterclockwise)

Preceding gene: 218708434

Following gene: 218708430

Centisome position: 12.2

GC content: 48.31

Gene sequence:

>1269_bases
ATGAAAGACGAAACGCTCTCGATTCACTTCGGCTACGACACTGATCCAACAACCAAATCGGTTGCGACCCCTATTTACCA
AACCGTTGCTTACGAATTCGATGACGCACAACACGGCGCCGACCTCTTCAATCTTGCGGTACCCGGTAATATCTACACTC
GCATAATGAACCCAACCAACGATGTGTTGGAAAAACGCATGGCGGCCTTAGAGGGTGGTATTGCAGGCTTAGTGGTGAGT
GCGGGCAGCGCGGCGATCAACTACGCCATTCAAACGTTGGCACAAATCGGTGATAACATCGTTTCAACGCCTCAGCTTTA
CGGCGGTACTTACACTCTATTTGCTCACATGCTGCCAAACCAAGGGATAGAAGTTCGCTTTGCCAAAGACGACAAACCAG
AAAGCTTAGCGGCACTGATCGATGACAAAACCAAAGCGGTTTACTGCGAAAGTATCGGTAACCCCGCTGGTAATATCATC
GACTTAGAACGTGTGGCTGAGCTTGCTCACGCACAAGGTGTACCTGTGATTGTCGATAACACGGTGGCGACACCTGTGTT
GTGTAAACCTATCGATTTTGGTGCTGATATTGTGGTGCACTCACTTACCAAATACGTTGGTGGTCACGGAACAACATTGG
GTGGCGTGATTGTCGACTCAGGCAAATTCCCATGGGCAGAGCACAAAGATCGCTTCCCAGTATTTAATCAGCCAGAACCT
TCTTACCATGGTGTAGTGTATACCGAAGCCTTTGGCGAAGCCGCGTTTATTGGCCGCGCTCGAACGGTTCCACTACGTAA
TACAGGTGCAGCATTGTCACCAATGAATGCCTTCATGCTAATGCAAGGTCTAGAAACGTTGTCGTTACGCATGGAGCGAC
ACACAGAGAACGCACTAAAAGTAGCAGAGTACCTTCAGCAACATGAAAAAGTGAGTTGGGTGAGCTATGCTGGTTTACCA
ACGTCTGAGTTCTATCCGTTGGCTGAGAAATACATGCAAGGTAAGCCGTCTGCCATTTTATCTTTTGGCTTGAAAGATGG
TTATGAAGCAGGTGTTCGTTTCTATGATGCGCTACAAATCTTCAAGCGCTTGGTGAACATTGGCGATGCAAAATCTCTCG
CTTGTCACCCTGCTTCTACAACACACCGTCAATTAAGCGAAGCGGAACAAAAGCAAGCAGGTGTGTCACCAGAGATGATC
CGCCTCTCTGTCGGCATTGAGCATATTGACGATATCTTGGCTGACCTAGAGCAAGCACTGAGCGCTTAA

Upstream 100 bases:

>100_bases
ACAATTCCAATTCCCTCTCTGTGACTAAATATACTTTTCTTTTATAAAAAAATCGGCAATATGAGAGTACATAGATAATT
CAATCGTTCAGGGAAGAAAC

Downstream 100 bases:

>100_bases
TGCTTAATGCTTAATGCTTAATGCTTAATGCTTAATGACGCTACAGCTAGCTCTAACACGAATCGTTAACTCACAGACAC
AAAAAAGCCCATCACAGGAT

Product: putative homocysteine synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKDETLSIHFGYDTDPTTKSVATPIYQTVAYEFDDAQHGADLFNLAVPGNIYTRIMNPTNDVLEKRMAALEGGIAGLVVS
AGSAAINYAIQTLAQIGDNIVSTPQLYGGTYTLFAHMLPNQGIEVRFAKDDKPESLAALIDDKTKAVYCESIGNPAGNII
DLERVAELAHAQGVPVIVDNTVATPVLCKPIDFGADIVVHSLTKYVGGHGTTLGGVIVDSGKFPWAEHKDRFPVFNQPEP
SYHGVVYTEAFGEAAFIGRARTVPLRNTGAALSPMNAFMLMQGLETLSLRMERHTENALKVAEYLQQHEKVSWVSYAGLP
TSEFYPLAEKYMQGKPSAILSFGLKDGYEAGVRFYDALQIFKRLVNIGDAKSLACHPASTTHRQLSEAEQKQAGVSPEMI
RLSVGIEHIDDILADLEQALSA

Sequences:

>Translated_422_residues
MKDETLSIHFGYDTDPTTKSVATPIYQTVAYEFDDAQHGADLFNLAVPGNIYTRIMNPTNDVLEKRMAALEGGIAGLVVS
AGSAAINYAIQTLAQIGDNIVSTPQLYGGTYTLFAHMLPNQGIEVRFAKDDKPESLAALIDDKTKAVYCESIGNPAGNII
DLERVAELAHAQGVPVIVDNTVATPVLCKPIDFGADIVVHSLTKYVGGHGTTLGGVIVDSGKFPWAEHKDRFPVFNQPEP
SYHGVVYTEAFGEAAFIGRARTVPLRNTGAALSPMNAFMLMQGLETLSLRMERHTENALKVAEYLQQHEKVSWVSYAGLP
TSEFYPLAEKYMQGKPSAILSFGLKDGYEAGVRFYDALQIFKRLVNIGDAKSLACHPASTTHRQLSEAEQKQAGVSPEMI
RLSVGIEHIDDILADLEQALSA
>Mature_422_residues
MKDETLSIHFGYDTDPTTKSVATPIYQTVAYEFDDAQHGADLFNLAVPGNIYTRIMNPTNDVLEKRMAALEGGIAGLVVS
AGSAAINYAIQTLAQIGDNIVSTPQLYGGTYTLFAHMLPNQGIEVRFAKDDKPESLAALIDDKTKAVYCESIGNPAGNII
DLERVAELAHAQGVPVIVDNTVATPVLCKPIDFGADIVVHSLTKYVGGHGTTLGGVIVDSGKFPWAEHKDRFPVFNQPEP
SYHGVVYTEAFGEAAFIGRARTVPLRNTGAALSPMNAFMLMQGLETLSLRMERHTENALKVAEYLQQHEKVSWVSYAGLP
TSEFYPLAEKYMQGKPSAILSFGLKDGYEAGVRFYDALQIFKRLVNIGDAKSLACHPASTTHRQLSEAEQKQAGVSPEMI
RLSVGIEHIDDILADLEQALSA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=31.2796208530806, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=28.6729857819905, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=27.790973871734, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1790375, Length=421, Percent_Identity=30.6413301662708, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789383, Length=426, Percent_Identity=24.6478873239437, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17538055, Length=421, Percent_Identity=34.2042755344418, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71980708, Length=410, Percent_Identity=29.0243902439024, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=373, Percent_Identity=34.0482573726542, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323333, Length=430, Percent_Identity=49.7674418604651, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319307, Length=375, Percent_Identity=33.8666666666667, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=26.2921348314607, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=433, Percent_Identity=27.0207852193995, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=320, Percent_Identity=24.6875, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=32.3040380047506, Blast_Score=187, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45805; Mature: 45805

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDETLSIHFGYDTDPTTKSVATPIYQTVAYEFDDAQHGADLFNLAVPGNIYTRIMNPTN
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCHH
DVLEKRMAALEGGIAGLVVSAGSAAINYAIQTLAQIGDNIVSTPQLYGGTYTLFAHMLPN
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHCCC
QGIEVRFAKDDKPESLAALIDDKTKAVYCESIGNPAGNIIDLERVAELAHAQGVPVIVDN
CCEEEEECCCCCCHHHHHHHCCCCCEEEEHHCCCCCCCEEEHHHHHHHHHHCCCCEEECC
TVATPVLCKPIDFGADIVVHSLTKYVGGHGTTLGGVIVDSGKFPWAEHKDRFPVFNQPEP
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCCCCCCCCCCCCC
SYHGVVYTEAFGEAAFIGRARTVPLRNTGAALSPMNAFMLMQGLETLSLRMERHTENALK
CCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEYLQQHEKVSWVSYAGLPTSEFYPLAEKYMQGKPSAILSFGLKDGYEAGVRFYDALQI
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHHHHH
FKRLVNIGDAKSLACHPASTTHRQLSEAEQKQAGVSPEMIRLSVGIEHIDDILADLEQAL
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCHHEEEEECCHHHHHHHHHHHHHHH
SA
CC
>Mature Secondary Structure
MKDETLSIHFGYDTDPTTKSVATPIYQTVAYEFDDAQHGADLFNLAVPGNIYTRIMNPTN
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCHH
DVLEKRMAALEGGIAGLVVSAGSAAINYAIQTLAQIGDNIVSTPQLYGGTYTLFAHMLPN
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHCCC
QGIEVRFAKDDKPESLAALIDDKTKAVYCESIGNPAGNIIDLERVAELAHAQGVPVIVDN
CCEEEEECCCCCCHHHHHHHCCCCCEEEEHHCCCCCCCEEEHHHHHHHHHHCCCCEEECC
TVATPVLCKPIDFGADIVVHSLTKYVGGHGTTLGGVIVDSGKFPWAEHKDRFPVFNQPEP
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCEECCEEEECCCCCCCCCCCCCCCCCCCCC
SYHGVVYTEAFGEAAFIGRARTVPLRNTGAALSPMNAFMLMQGLETLSLRMERHTENALK
CCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEYLQQHEKVSWVSYAGLPTSEFYPLAEKYMQGKPSAILSFGLKDGYEAGVRFYDALQI
HHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHHHHHH
FKRLVNIGDAKSLACHPASTTHRQLSEAEQKQAGVSPEMIRLSVGIEHIDDILADLEQAL
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCHHEEEEECCHHHHHHHHHHHHHHH
SA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]